Integrated assessment with biomarker responses and metal concentrations on some fish species from İzmir Bay: A preliminary investigation

Mustafa Bilgin; Esin Uluturhan Suzer; Enis Darılmaz

doi:10.12714/egejfas.39.4.03

TR EN

İzmir Körfezi’ndeki bazı balık türlerinde biyoişaretçi yanıtlarının metal konsantrasyonları ile birlikte değerlendirilmesi: Bir ön araştırma

Öz

İzmir Körfezi’ndeki balık türlerinin (Sparus aurata, Chelon labrosus, Diplodus vulgaris) organlarında metal (Hg, Cd, Pb, Cr, Cu, Zn ve Mn) biyobirikimini değerlendirmek için antioksidanla kökenli biyoişaretçiler (süperoksit dismutaz, katalaz, malondialdehit) incelenmiştir. Balıklar Kasım 2019'da İç ve Dış Körfez’den toplanmıştır. Metal ve biyoişaretçi analizleri sırasıyla Atomik Absorpsiyon Spektrometresi ve mikroplaka okuyucu ile yapılmıştır. Organlarda bulunan metal değerlerine göre karaciğer dokularında daha yüksek metal biyobirikimleri bulunmuştur. S. aurata'da daha yüksek Hg ve Zn konsantrasyonları, C. labrosus'ta daha yüksek Cd, Cr, Cu ve Mn konsantrasyonları ve D. vulgaris'te ise daha yüksek Pb konsantrasyonları tespit edilmiştir. Biyoişaretçi sonuçlarına göre, solungaçlarda SOD ve CAT aktiviteleri genel olarak daha yüksek, ancak MDA içerikleri karaciğerde daha yüksek bulunmuştur. En yüksek biyoişaretçi bulguları ise C. labrosus'ta tespit edilmiştir. İstatistiksel hesaplamalar, özellikle MDA içeriğinin, metal biyobirikimleri için kayda değer cevaplar verdiğini göstermiştir. Ayrıca Mn seviyeleri, beklendiği gibi tüm biyoişaretçiler ile anlamlı korelasyonlar göstermiştir. Bu çalışma, biyoişaretçilerin ve metal konsantrasyonlarının birlikte kullanımının deniz ekosistemlerinin sağlık durumunu araştırmak için önemli bir belirteç olabileceğini ortaya koymuştur.

Anahtar Kelimeler

Integrated assessment with biomarker responses and metal concentrations on some fish species from İzmir Bay: A preliminary investigation

Abstract

Antioxidant related biomarkers (superoxide dismutase, catalase, malondialdehyde) were investigated to evaluate metal (Hg, Cd, Pb, Cr, Cu, Zn, and Mn) bioaccumulation in some organs of fish species (Sparus aurata, Chelon labrosus, Diplodus vulgaris) from the İzmir Bay. Samples were collected at November 2019 from Inner and Outer Bays. Metal and biomarker analyses were carried out by Atomic Absorption Spectrometer and microplate reader, respectively. For metal analyses in organs, higher metal bioaccumulations were found at liver tissues. Higher Hg and Zn concentrations were found in S. aurata, higher Cd, Cr, Cu and, Mn concentrations were detected in C. labrosus and higher Pb concentrations were determined in D. vulgaris. In biomarker results, superoxide dismutase (SOD) and catalase (CAT) activities were generally higher in gills, however, MDA contents were higher at liver. The highest biomarker results were detected at C. labrosus. Statistical analyses were demonstrated that especially MDA content were expressed strong responses for the metal bioaccumulations. Also, Mn levels were considerably correlated with all biomarkers as expected. This study revealed that combined utilization of biomarkers and metal concentrations could be a vital indicator to investigate health status of the marine ecosystems.

Keywords

Supporting Institution

Dokuz Eylül University

Project Number

2019.KB.FEN.040

Thanks

This study is represented a part of the PhD thesis of Mustafa BİLGİN. The study was supported by Dokuz Eylül University, Department of Scientific Research Projects (Project Number: 2019.KB.FEN.040). Authors would like to thank to their colleagues for the contributions at the laboratory and the field

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Details

Primary Language

English

Subjects

Maritime Engineering (Other)

Journal Section

Research Article

Authors

Mustafa Bilgin
0000-0003-4680-5931
Türkiye

Esin Uluturhan Suzer ^*
0000-0002-7886-922X
Türkiye

Enis Darılmaz
0000-0002-9090-9319
Türkiye

Publication Date

December 15, 2022

Submission Date

June 27, 2022

Acceptance Date

September 6, 2022

Published in Issue

Year 2022 Volume: 39 Number: 4

DOI

https://doi.org/10.12714/egejfas.39.4.03

IZ

https://izlik.org/JA46XJ92KT

Cite

RIS / Bibtex

APA

Bilgin, M., Uluturhan Suzer, E., & Darılmaz, E. (2022). Integrated assessment with biomarker responses and metal concentrations on some fish species from İzmir Bay: A preliminary investigation. Ege Journal of Fisheries and Aquatic Sciences, 39(4), 284-292. https://doi.org/10.12714/egejfas.39.4.03