Farklı su sıcaklıklarındaki pullu sazan (Cyprinus carpio Linnaeus, 1758)’ da polenin antioksidan etkisinin araştırılması

Merve Taşkan; M. Enis Yonar

doi:10.12714/egejfas.40.1.10

EN TR

Investigation of antioxidant effect of pollen in scaly carp (Cyprinus carpio Linnaeus, 1758) in different water temperature

Abstract

In this study, it was aimed to investigate changes in some immunological and antioxidant parameters in scaly carp (Cyprinus carpio) added pollen to their feed in different water temperatures. Fish were stocked to glass aquariums adjusted to 18 °C, 23 °C and 28 °C water temperature. Fish were given diets containing % 2,5 pollen for 14 days. Blood and tissue samples were analysed to determine the immunological parameters and oxidant/antioxidant status. When compared to the control group (23 ºC), a statistically significant increase in the NBT activity of the groups at 18 ºC was observed. Decrease in the NBT activity of the group at 28 º C was statistically insignificant. The total protein and total immunoglobulin levels of the group at 18 º C were decreased, while increase in both parameters at 28 º C was not significant. When compared to the control group (23 ºC), the NBT activity, the total protein and total immunoglobulin levels in the groups that maintained at the same temperature with the control group (23 º C) and applied pollen did not show any statistically significant difference. The tissue MDA levels were increased in the groups at 18 °C and 28 °C when compared to the control group (23 º C). The tissue MDA levels of the groups treated pollen simultaneously with the change in temperature were lower than the groups at 18 °C and 28 °C. The tissue GSH levels and GST activities were decreased in the groups at 18 °C and 28 °C when compared to the control group (23 º C). The tissue GSH levels and GST activities of the groups treated pollen simultaneously with the change in temperature were higher than the groups at 18 °C and 28 °C. In conclusion, stress caused by temperature differences in fish may be prevented by pollen.

Keywords

Farklı su sıcaklıklarındaki pullu sazan (Cyprinus carpio Linnaeus, 1758)’ da polenin antioksidan etkisinin araştırılması

Öz

Bu çalışmada farklı su sıcaklıklarında yemlerine polen ilave edilmiş pullu sazanda bazı immunolojik ve antioksidan parametrelerdeki değişimlerin araştırılması amaçlandı. Balıklar su sıcaklığı 18 oC, 23 oC ve 28 oC’ ye ayarlanmış akvaryumlara stoklandı. Balıklara % 2,5 oranında polen içeren yemler 14 gün süreyle verildi. Balıklardan alınan kan ve doku örneklerinde immunolojik ve oksidan/antioksidan parametreler analiz edildi. Kontrol grubu (23 ºC) ile kıyaslandığında, 18 ºC' deki grubun NBT aktivitesinde istatistiksel olarak önemli bir artış belirlendi. 28 ºC' deki grubun NBT aktivitesinde belirlenen azalma istatistiksel olarak önemsiz bulundu. 18 ºC' deki grubun total protein ve total immunoglobulin düzeyleri azalırken, 28 ºC' deki grupta her iki parametre için belirlenen artış önemsiz bulundu. Kontrol grubu (23 ºC)' na kıyasla 18 ºC ve 28 ºC sıcaklıkta tutulan ve polen uygulanan grupların NBT aktivitesi ile total protein ve total immunoglobulin düzeylerinin kontrol grubundan istatistiksel olarak önemli herhangi bir farklılık göstermediği belirlendi. Kontrol grubu (23 ºC)' na kıyasla, 18 ºC ve 28 ºC' deki grupların doku MDA düzeyleri arttı. Sıcaklıktaki değişimle eşzamanlı olarak polen uygulanan grupların MDA düzeyleri 18 ºC ve 28 ºC' deki gruplardan daha düşüktü. Kontrol grubu (23 ºC) ile kıyaslandığında, 18 ºC ve 28 ºC' deki grupların doku GSH düzeyleri ve GST aktiviteleri azaldı. Sıcaklıktaki değişimle eşzamanlı olarak polen uygulanan gruplarda GSH düzeyleri ve GST aktiviteleri 18 ºC ve 28 ºC' deki gruplardan daha yüksekti. Sonuç olarak balıklarda sıcaklık farklılıklarından kaynaklanan stres polenle önlenebilir.

Anahtar Kelimeler

Supporting Institution

Fırat Üniversitesi Bilimsel Araştırma Projeleri (FÜBAP) Yönetim Birimi

Project Number

SÜF.16.07

Thanks

BBu çalışma; Yüksek Mühendis Merve TAŞKAN' ın yüksek lisans tezinden özetlenmiş ve Fırat Üniversitesi Bilimsel Araştırma Projeleri (FÜBAP) Yönetim Birimi tarafından SÜF.16.07. nolu proje olarak desteklenmiştir. Polen örneklerinin palinolojik olarak identifikasyonu için Erciyes Üniversitesi Seyrani Ziraat Fakültesi' nden Prof. Dr. Sibel SİLİCİ’ ye teşekkür ederiz.

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Details

Primary Language

Turkish

Subjects

Zoology

Journal Section

Research Article

Authors

Merve Taşkan
0000-0002-5105-7010
Türkiye

M. Enis Yonar ^*
0000-0001-9519-4247
Türkiye

Publication Date

March 15, 2023

Submission Date

May 16, 2022

Acceptance Date

July 18, 2022

Published in Issue

Year 2023 Volume: 40 Number: 1

DOI

https://doi.org/10.12714/egejfas.40.1.10

IZ

https://izlik.org/JA74PN93HR

Cite

RIS / Bibtex

APA

Taşkan, M., & Yonar, M. E. (2023). Farklı su sıcaklıklarındaki pullu sazan (Cyprinus carpio Linnaeus, 1758)’ da polenin antioksidan etkisinin araştırılması. Ege Journal of Fisheries and Aquatic Sciences, 40(1), 69-76. https://doi.org/10.12714/egejfas.40.1.10