Diagnosis of bacterial fish diseases and classification of serotypes with slide agglutination method

Kaan Kumaş; Prof.dr. T. Tansel Tanrıkul

doi:10.12714/egejfas.39.2.02

TR EN

Lam aglütinasyon metodu ile bakteriyel balık hastalıklarının teşhisi ve serotiplerin sınıflandırılması

Öz

Bakteriyel balık patojenleri gökkuşağı alabalığı yetiştiriciliği yapan balık çiftliklerinde ciddi kayıplara sebep olmaktadır. Su ürünleri yetiştiriciliğinde hastalık etkeni bakteriyel patojenler, oluşan hastalıkların teşhisi için hızlı teşhis yöntemlerinin gelişmesine yol açmıştır. Hastalıklar yetiştiricilik tesislerinde görüldükten sonra balık patojenlerinin hızlı teşhis edilmesi, hastalıkların tedavi edilerek oluşacak ekonomik kayıpların önüne geçilmesi için önemlidir. Balık hastalıklarının hızlı teşhisinde az miktarda antiserum ve örnek ile uygulanabilen serolojik teknikler tercih edilmektedir. Bu çalışmada Lactococcus garvieae, Vibrio (Listonella) anguillarum ve Yersinia ruckeri’nin referans suşlarından antijenler kullanılmak üzere formalin ile inaktive edilerek, Yeni Zelanda tavşanlarına ardışık dozlarda intravenöz enjeksiyonlar gerçekleştirilmiştir. Son enjeksiyon yapıldıktan bir hafta sonra antiserum elde edilmiştir. 18 Y. ruckeri, 14 V. anguillarum ve 7 L. garvieae ve referans (ATCC 43305, ATCC 29473, ATCC 49156) suşlar dahil olmak üzere toplamda 42 suş ile çalışma yapılmıştır. Lam aglütinasyon metodu ile incelenen V. anguillarum suşlarında baskın olarak (%86) serotip O1, Y. ruckeri suşlarında (%84) serotip O1 tespit edilmiştir. L. garvieae suşlarının Japon KG- antiserumu ile aglütinasyon vermediği, ancak Türk KG- antiserumu ile pozitif reaksiyon oluşturduğu tespit edilmiştir.

Anahtar Kelimeler

Diagnosis of bacterial fish diseases and classification of serotypes with slide agglutination method

Abstract

Bacterial fish pathogens cause significant losses in rainbow trout farms. In fish farms, bacterial pathogens cause threatening diseases which have made necessary to develop rapid methods for disease diagnosis. Serological techniques which are applied with a small amount of antiserum and sample, are preferred for the rapid diagnosis of fish diseases. In this study, formalin-killed antigens prepared from reference strains of Lactococcus garvieae, Yersinia ruckeri, and Vibrio (Listonella) anguillarum were injected intravenously in consecutive doses to New Zealand rabbits. One week after the last injection, the sera separated to use in the slide agglutination tests. A total of 42 strains were studied, including Y. ruckeri (18 isolates), V. anguillarum (14 isolates), and L. garvieae (7 isolates) and 3 reference (ATCC 43305, ATCC 29473, ATCC 49156) strains. Serotype O1 determined predominant serotype (86%) in V. anguillarum and Y. ruckeri (84%) strains examined by slide agglutination method. L. garvieae strains did not react against Japanese antisera but positively reacted against Turkish L. garvieae antisera.

Keywords

Supporting Institution

Izmir Katip Celebi University Scientific Research Projects Coordinatorship

Project Number

2021-TYL-FEBE-0012

Thanks

This study presents a part of Kaan KUMAŞ’s master thesis titled “Diagnosis of bacterial fish diseases with using slide agglutination method” (with English abstract) and was supported by İzmir Katip Çelebi University Scientific Research Projects Coordinatorship with the project number 2021-TYL-FEBE-0012.

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Kaan Kumaş ^*
0000-0003-1583-8482
Türkiye

Prof.dr. T. Tansel Tanrıkul
0000-0002-1684-7965
Türkiye

Publication Date

June 15, 2022

Submission Date

January 21, 2022

Acceptance Date

March 21, 2022

Published in Issue

Year 2022 Volume: 39 Number: 2

DOI

https://doi.org/10.12714/egejfas.39.2.02

IZ

https://izlik.org/JA82AA42EK

Cite

RIS / Bibtex

APA

Kumaş, K., & Tanrıkul, P. T. T. (2022). Diagnosis of bacterial fish diseases and classification of serotypes with slide agglutination method. Ege Journal of Fisheries and Aquatic Sciences, 39(2), 97-103. https://doi.org/10.12714/egejfas.39.2.02