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Hematology of the Uludağ Frog, Rana macrocnemis Boulenger, 1885 in Uludağ National Park (Bursa, Turkey)

Year 2008, Volume: 25 Issue: 1, 39 - 46, 01.03.2008

Suha Kenan Arserim Ahmet Mermer

Abstract

Seasonal change of some hematological parameters and cytomorphometry of blood cells (erythrocyte, leukocyte and thrombocyte) were studied statistically and histologically in a total of 30 (15 ♂♂, 15 ♀♀) adult individuals of Rana macrocnemis from Uludağ National Park. In 1 mm3 of blood, erythrocyte count was estimated as 514,839 (280,000 – 940,000), leukocyte count as 3,527 (2,600 – 5,200), thrombocyte count as 31,069 (20,000 – 50,000), hemoglobin amount (Hb) as 8.10 (5.60 – 12.10) g/dl, hematocrit value (HCT) as 0.34 (0.16 – 0.46) L/l, mean cell volume (MCV) as 694.54 (420.45 – 1105.26) , mean cell hemoglobin (MCHb) as 167.88 (87.78 – 257.89) pg and mean cell hemoglobin concentration (MCHbC) as 251.43 (160.87 – 387.50) g/l. Seasonal changes were detected in erythrocyte count and related parameters (Hb, HCT, MCV, MCHb, and MCHbC). There are small differences between sexes in terms of these values although they are statistically insignificant. Nevertheless, neither seasonal nor sex-based variations were observed in leukocyte and thrombocyte count values. Small seasonal differences are observed in terms of cell sizes although they are concluded to be individual variations. 

Keywords

Uludağ National Park Turkey Rana macrocnemis hematology blood cells

References

  • Alder, A., E. Huber. 1923. Unstersucheng über blutcellen bei Amphibien und Reptilien. Folia Heamatol. 29:1-22.
  • Arıkan, H. 1989. Investigation on Rana ridibunda (Anura, Ranidae) populations aspect of blood cells, (in Turkish). Turk. J. Zool. 13: 54-59.
  • Arıkan H., İ.E. Çevik, U. Kaya, and A. Mermer. 2001. Erythrocytes measurements on Anatolian mountain frogs, (in Turkish). Anadolu Ünv. Bilim ve Teknoloji Dergisi 2: 387-391.
  • Arvy, L. 1947. Le dimorphisme sexuel sanguin chez Rana temporaria L. et Bufo vulgaris. Laur. Compt. Rend. Soc. Biol. 141: 457-459.
  • Atatür, M.K., H. Arıkan, and İ.E. Çevik. 1999. Erythrocyte sizes of some Anurans from Turkey. Turk. J. Zool. 23: 111-114.
  • Banerjee, V. 1988. Erythrocyte related blood parameters in Bufo melanostictus with reference to sex and body weight. Environ. Ecol. Kalyani. 6: 802-806.
  • Barni, S., G. Bernocchi. 1991. Internalization of erythrocytes into liver parenchymal cells in naturally hibernating frogs (Rana esculenta L.). J. Exp. Zool. 258: 143-150.
  • Barni, S., R. Nano, V. Bertone, and E. Prosperi. 1992. Ultrastructure and cytochemistry of circulating erythrocytes during the annual cycle of Rana esculenta L. Comp. Haematol. Int. 2: 166-169.
  • Başoğlu, M., N. Özeti. 1973. The Amphibians of Turkey, (in Turkish). Ege Üniversitesi Fen Fakültesi Kitapları Serisi, No. 151, Bornova-İzmir. 155 pp.
  • Blaxhall, P.C., K.W. Daisley. 1973. Routine haematological methods for use fish with blood. J. Fish Biol. 5: 771-781.
  • Choubey, B.G., A. Shankar, and B.J. Choubey. 1986. Haematological investigation of Himalayan toad Bufo melanostictus Schneider in relation to sex and size. Biol. Bull. India. 8: 106-114.
  • Conroy, D.A. 1972. Studies on the haematology of the Atlantic Aalmon (Salmo salar L.). Symp. Zool. Soc. Lond. 30: 101-127.
  • Evans, G. 1939. Factors in fluencing the oxygen consumption of several species of plethodontid salamanders in aerial aquatic media. Ecology 20: 74-95.
  • Foxon, G.E.H. 1964. Blood and respiration, pp. 151-209. In J.A. Moore [ed.], Physiology of the Amphibia. Academic Press.
  • Glomski, C.A., J. Tamburlin, R. Hard, and M. Chainami. 1997. The phylogenetic odyssey of the erythrocyte. IV. the Amphibians. Histol. Histopathol. 12: 147-170.
  • Goniakowska, L. 1970. The respiration of erythrocytes of some amphibians in vitro. Bull. Acad. Polon. Sci., Sér. Sci. Biol. 12: 793-797.
  • Goniakowska, L. 1973. Metabolism, resistance to hypotonic solutions, and ultrastructure of erythrocytes of five amphibian species. Acta. Biol. Crac., Ser. Zool. 13: 225-236.
  • Hutchison, V.H., H. Szarski. 1965. Number of erythrocytes in some amphibians and reptiles. Copeia 3: 373-375.
  • Ishchenko, V. 1978. Dinamicheskij polimorfism burikh lyagushek fauni SSSR [Dynamic polymorphism of brown frogs of fauna of USSR], Nauka Publ. House.
  • Jones, B.J., W.D. Pearson. 1976. Variations in haematocrit values of successive blood samples from Bluegill. Trans. Am. Fish. Soc. 2: 291- 293.
  • Kaloustian, K.V., R.W. Dulac. 1982. Relationship between red blood cell indices and the effect of thyroxine in three species of amphibians. Comp. Biochem. Physiol. 73A: 427-430
  • Kaplan, H.M. 1951. A study of frog blood in red leg disease. Trans. III. State Acad. Sci 44: 209-215.
  • Kaplan, H.M. 1952. Variations in white blood cells between normal and red leg frogs. Ibid. 45: 170-176.
  • Klieneberger, C. 1927. Die Blutmorphologie der laboratoriumstiere, Barth, Liepzig.
  • Kocabatmaz, M., G. Ekingen. 1984. Taking blood sample in different fish species and standardization of hematological methods, (in Turkish). Doğa Bilim Dergisi 8: 149-159.
  • Kuramoto, M. 1981. Relationships between number, size and shape of red blood cells in amphibians. Comp. Physiol. 69: 771-775.
  • Nano, R., P. Griffini, and S. Barni. 1991. Morphohistochemical changes of the blood cells in the hibernating frog (Rana esculenta L.). Comp. Haematol. Int. 1: 220-223.
  • Pai, R.M., S.V. Shanbhag. 1988. On erythrocytes of certain Indian amphibians. Comp. Physiol. Ecol. 13: 103-110.
  • Prosser, C.L., J.F. Weinstein. 1950. Comparison of blood volume in animals with open or closed circulatory systems. Physiol. Zool. 23: 113-124.
  • Reddy, P., M. Bashamohideen. 1989. Fenvalarate and cypermethrin induced changes in the haematological parameters of Cyprinus carpio. Acta. Hydrochim. Hydrobiol. 17: 101-107.
  • Rouf, M.A. 1969. Hematology of the Leopard frog, Rana pipiens. Copeia 4: 682-687.
  • Ruiz, G., M. Rosenmann, and A. Veloso. 1983. Respiratory and hematological adaptations to high altitude in Telmatobius frogs from the Chilean Andes. Comp. Biochem. Physiol. 76A: 109-113.
  • Ruiz, G., M. Rosenmann, and A. Veloso. 1989. Altitudinal distribution and blood values in the toad, Bufo spinulosus Wiegmann. Comp. Biochem. Physiol. 94A: 643-646.
  • Samantaray, K. 1985. Studies on hematology of Indian skipper frog, Rana cyanophlyctis Schneider. Comp. Physiol. Ecol. 10: 71-74.
  • Satake, T., A. Nuti-Sobrinho, O.V. Paula-Lopes, R.A. Lopes, and H.S. Leme Dos Santos. 1986. Haematological study of Brazilian fish. III. Blood parameters in Armored Catfish Hypostomus paulinus I. Ars Veterinaria, Faculdade de Ciências Agrárias e Veterinárias “Campus” de Jaboticabal Unesp 2: 179-183.
  • Schermer, S. 1954. Die Blutmorphologie der laboratorium stiere, Barth.
  • Seiverd, C.E. 1964. Hematology for medical technologists, Lea and Febiger.
  • Sinha, R.C. 1983. Haematological studies on the prewintering and wintering frog, Rana esculenta. Comp. Biochem. Physiol. 74A: 311-314.
  • Snieszko, S.F. 1960. Microhaematocrit as a tool in fishery research and management. U. S. Wildl. Serv. Sci. Rep. Fish. 341: 1-5.
  • Stephan, F. 1954. Morphologie générale du systéme circulatoire, pp. 854– 973. In P.P. Grasse (ed.), Traitéde zoologie, Masson.
  • Szarski, H., G. Czopek, 1966. Erythrocyte diameter in some Amphibians and Reptiles. Bull. Acad. Pol. Sci. Cl. 2 Ser. Sci. Biol. 14: 433-437.
  • Tarkhnishvili, D.N., R.K. Gokhelashvili. 1999. The Amphibians of the Caucasus, Pensoft Publications.
  • Wintrobe, M.M. 1933. Variations in the size and hemoglobin content of erythrocytes in the blood of various vertebrates. Folia Haematologie 51: 32-49.
  • Wojtaszek, J., A. Adamowicz. 2003. Haematology of the fire-bellied toad, Bombina bombina L. Comp. Clin. Path. 12: 129-139.
  • Wojtaszek, J., M. Baranowska, M. Glubiak, and A. Dżugaj. 1997. Circulating blood parameters of the water frog, Rana esculenta L. at pre-wintering stage. Zool. Pol. 1–4: 117-126.
  • Zhukova, T.I., B.S. Kubantsev. 1979. Changes in the blood composition of salientians during hibernation. Sov. J. Ecol. 9: 379-382.

Uludağ Milli Parkı’nda uludağ kurbağası, Rana macrocnemis Boulenger, 1885’in hematolojisi (Bursa, Türkiye)

Year 2008, Volume: 25 Issue: 1, 39 - 46, 01.03.2008

Suha Kenan Arserim Ahmet Mermer

Abstract

Uludağ Milli Parkı’nda uludağ kurbağası, Rana macrocnemis Boulenger, 1885’in hematolojisi (Bursa, Türkiye). Uludağ Milli Parkı’ından, 30 (15 ♂♂, 15 ♀♀) ergin Rana macrocnemis örneğinde bazı hematolojik parametreler ve periferal kan hücrelerinin (eritrosit, lökosit, trombosit) morfolojisindeki mevsimsel değişimler istatistiksel ve histolojik yöntemlerle incelenmiştir. 1 mm3 kandaki eritrosit sayısı, 514,839 (280,000 – 940,000); lökosit sayısı, 3,527 (2,600 – 5,200); trombosit sayısı, 31,069 (20,000 – 50,000); hemoglobin miktarı (Hb), 8.10 (5.60 – 12.10) g/dl; hematokrit değeri (HCT), 0.34 (0.16 – 0.46) L/l; ortalama hücre hacmi (MCV), 694.54 (420.45 – 1105.26) fl; ortalama hücre hemoglobini (MCHb), 167.88 (87.78 – 257.89) pg; ortalama hücre hemoglobin konsantrasyonu (MCHbC), 251.43 (160.87 – 387.50) g/l olarak hesaplanmıştır. Eritrosit sayısı ile ilgili parametreler açısından (Hb, HCT, MCV, MCHb ve MCHbC) mevsimsel değişimler saptanmıştır. Bu değerler açısından cinsiyetler arasında da küçük farklılıklar olsa da istatistiki açından önemsizdir. Buna karşın, lökösit ve trombosit sayısı değerleri açısından gerek mevsimsel ve gerekse cinsiyete bağlı varyasyonlar gözlenmemiştir. Hücre büyüklükleri açısından küçük mevsimsel farklılıklar tespit edilmiştir. Fakat bunların bireysel varyasyonlar olduğu sonucuna varılmıştır

Keywords

Uludağ Milli Parkı Türkiye Rana macrocnemis Hematoloji Kan Hücreleri

References

  • Alder, A., E. Huber. 1923. Unstersucheng über blutcellen bei Amphibien und Reptilien. Folia Heamatol. 29:1-22.
  • Arıkan, H. 1989. Investigation on Rana ridibunda (Anura, Ranidae) populations aspect of blood cells, (in Turkish). Turk. J. Zool. 13: 54-59.
  • Arıkan H., İ.E. Çevik, U. Kaya, and A. Mermer. 2001. Erythrocytes measurements on Anatolian mountain frogs, (in Turkish). Anadolu Ünv. Bilim ve Teknoloji Dergisi 2: 387-391.
  • Arvy, L. 1947. Le dimorphisme sexuel sanguin chez Rana temporaria L. et Bufo vulgaris. Laur. Compt. Rend. Soc. Biol. 141: 457-459.
  • Atatür, M.K., H. Arıkan, and İ.E. Çevik. 1999. Erythrocyte sizes of some Anurans from Turkey. Turk. J. Zool. 23: 111-114.
  • Banerjee, V. 1988. Erythrocyte related blood parameters in Bufo melanostictus with reference to sex and body weight. Environ. Ecol. Kalyani. 6: 802-806.
  • Barni, S., G. Bernocchi. 1991. Internalization of erythrocytes into liver parenchymal cells in naturally hibernating frogs (Rana esculenta L.). J. Exp. Zool. 258: 143-150.
  • Barni, S., R. Nano, V. Bertone, and E. Prosperi. 1992. Ultrastructure and cytochemistry of circulating erythrocytes during the annual cycle of Rana esculenta L. Comp. Haematol. Int. 2: 166-169.
  • Başoğlu, M., N. Özeti. 1973. The Amphibians of Turkey, (in Turkish). Ege Üniversitesi Fen Fakültesi Kitapları Serisi, No. 151, Bornova-İzmir. 155 pp.
  • Blaxhall, P.C., K.W. Daisley. 1973. Routine haematological methods for use fish with blood. J. Fish Biol. 5: 771-781.
  • Choubey, B.G., A. Shankar, and B.J. Choubey. 1986. Haematological investigation of Himalayan toad Bufo melanostictus Schneider in relation to sex and size. Biol. Bull. India. 8: 106-114.
  • Conroy, D.A. 1972. Studies on the haematology of the Atlantic Aalmon (Salmo salar L.). Symp. Zool. Soc. Lond. 30: 101-127.
  • Evans, G. 1939. Factors in fluencing the oxygen consumption of several species of plethodontid salamanders in aerial aquatic media. Ecology 20: 74-95.
  • Foxon, G.E.H. 1964. Blood and respiration, pp. 151-209. In J.A. Moore [ed.], Physiology of the Amphibia. Academic Press.
  • Glomski, C.A., J. Tamburlin, R. Hard, and M. Chainami. 1997. The phylogenetic odyssey of the erythrocyte. IV. the Amphibians. Histol. Histopathol. 12: 147-170.
  • Goniakowska, L. 1970. The respiration of erythrocytes of some amphibians in vitro. Bull. Acad. Polon. Sci., Sér. Sci. Biol. 12: 793-797.
  • Goniakowska, L. 1973. Metabolism, resistance to hypotonic solutions, and ultrastructure of erythrocytes of five amphibian species. Acta. Biol. Crac., Ser. Zool. 13: 225-236.
  • Hutchison, V.H., H. Szarski. 1965. Number of erythrocytes in some amphibians and reptiles. Copeia 3: 373-375.
  • Ishchenko, V. 1978. Dinamicheskij polimorfism burikh lyagushek fauni SSSR [Dynamic polymorphism of brown frogs of fauna of USSR], Nauka Publ. House.
  • Jones, B.J., W.D. Pearson. 1976. Variations in haematocrit values of successive blood samples from Bluegill. Trans. Am. Fish. Soc. 2: 291- 293.
  • Kaloustian, K.V., R.W. Dulac. 1982. Relationship between red blood cell indices and the effect of thyroxine in three species of amphibians. Comp. Biochem. Physiol. 73A: 427-430
  • Kaplan, H.M. 1951. A study of frog blood in red leg disease. Trans. III. State Acad. Sci 44: 209-215.
  • Kaplan, H.M. 1952. Variations in white blood cells between normal and red leg frogs. Ibid. 45: 170-176.
  • Klieneberger, C. 1927. Die Blutmorphologie der laboratoriumstiere, Barth, Liepzig.
  • Kocabatmaz, M., G. Ekingen. 1984. Taking blood sample in different fish species and standardization of hematological methods, (in Turkish). Doğa Bilim Dergisi 8: 149-159.
  • Kuramoto, M. 1981. Relationships between number, size and shape of red blood cells in amphibians. Comp. Physiol. 69: 771-775.
  • Nano, R., P. Griffini, and S. Barni. 1991. Morphohistochemical changes of the blood cells in the hibernating frog (Rana esculenta L.). Comp. Haematol. Int. 1: 220-223.
  • Pai, R.M., S.V. Shanbhag. 1988. On erythrocytes of certain Indian amphibians. Comp. Physiol. Ecol. 13: 103-110.
  • Prosser, C.L., J.F. Weinstein. 1950. Comparison of blood volume in animals with open or closed circulatory systems. Physiol. Zool. 23: 113-124.
  • Reddy, P., M. Bashamohideen. 1989. Fenvalarate and cypermethrin induced changes in the haematological parameters of Cyprinus carpio. Acta. Hydrochim. Hydrobiol. 17: 101-107.
  • Rouf, M.A. 1969. Hematology of the Leopard frog, Rana pipiens. Copeia 4: 682-687.
  • Ruiz, G., M. Rosenmann, and A. Veloso. 1983. Respiratory and hematological adaptations to high altitude in Telmatobius frogs from the Chilean Andes. Comp. Biochem. Physiol. 76A: 109-113.
  • Ruiz, G., M. Rosenmann, and A. Veloso. 1989. Altitudinal distribution and blood values in the toad, Bufo spinulosus Wiegmann. Comp. Biochem. Physiol. 94A: 643-646.
  • Samantaray, K. 1985. Studies on hematology of Indian skipper frog, Rana cyanophlyctis Schneider. Comp. Physiol. Ecol. 10: 71-74.
  • Satake, T., A. Nuti-Sobrinho, O.V. Paula-Lopes, R.A. Lopes, and H.S. Leme Dos Santos. 1986. Haematological study of Brazilian fish. III. Blood parameters in Armored Catfish Hypostomus paulinus I. Ars Veterinaria, Faculdade de Ciências Agrárias e Veterinárias “Campus” de Jaboticabal Unesp 2: 179-183.
  • Schermer, S. 1954. Die Blutmorphologie der laboratorium stiere, Barth.
  • Seiverd, C.E. 1964. Hematology for medical technologists, Lea and Febiger.
  • Sinha, R.C. 1983. Haematological studies on the prewintering and wintering frog, Rana esculenta. Comp. Biochem. Physiol. 74A: 311-314.
  • Snieszko, S.F. 1960. Microhaematocrit as a tool in fishery research and management. U. S. Wildl. Serv. Sci. Rep. Fish. 341: 1-5.
  • Stephan, F. 1954. Morphologie générale du systéme circulatoire, pp. 854– 973. In P.P. Grasse (ed.), Traitéde zoologie, Masson.
  • Szarski, H., G. Czopek, 1966. Erythrocyte diameter in some Amphibians and Reptiles. Bull. Acad. Pol. Sci. Cl. 2 Ser. Sci. Biol. 14: 433-437.
  • Tarkhnishvili, D.N., R.K. Gokhelashvili. 1999. The Amphibians of the Caucasus, Pensoft Publications.
  • Wintrobe, M.M. 1933. Variations in the size and hemoglobin content of erythrocytes in the blood of various vertebrates. Folia Haematologie 51: 32-49.
  • Wojtaszek, J., A. Adamowicz. 2003. Haematology of the fire-bellied toad, Bombina bombina L. Comp. Clin. Path. 12: 129-139.
  • Wojtaszek, J., M. Baranowska, M. Glubiak, and A. Dżugaj. 1997. Circulating blood parameters of the water frog, Rana esculenta L. at pre-wintering stage. Zool. Pol. 1–4: 117-126.
  • Zhukova, T.I., B.S. Kubantsev. 1979. Changes in the blood composition of salientians during hibernation. Sov. J. Ecol. 9: 379-382.
There are 46 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Suha Kenan Arserim

Ahmet Mermer

Publication Date March 1, 2008
Submission Date December 7, 2015
Published in Issue Year 2008 Volume: 25 Issue: 1

Cite

APA Arserim, S. K., & Mermer, A. (2008). Hematology of the Uludağ Frog, Rana macrocnemis Boulenger, 1885 in Uludağ National Park (Bursa, Turkey). Ege Journal of Fisheries and Aquatic Sciences, 25(1), 39-46.