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Effect of natural adsorbent Clinoptilolite on some hematological parameters of Cyprinus carpio

Year 2018, Volume: 35 Issue: 4, 455 - 462, 15.12.2018

Okay Oğuztürk Nuray Çiftçi , Bedii Cicik

https://doi.org/10.12714/egejfas.2018.35.4.11

Abstract



Effect of natural chelating agent clinoptilolite on hematocrit level, erythrocyte numbers, mean corpuscular volume (MCV), erythrocyte and erythrocyte nucleus area of Cyprinus carpio were determined after exposing the animals to 2, 4, 8, 16, 32 gL-1 clinoptilolite (0.40 µ) (3, 7, 21 and 30 days). Hematocrit levels were determined using microhematocrit methods and erythrocyte numbers, erythrocyte and its nucleus areas and MCV were determined using microscopic methods.



Erythrocyte number and hematocrit levels decreased at 16 and 32 g L-1 of clinoptilolite with compared to control group in except 3 and 7 days of exposure (p<0.05). Clinoptilolite increased MCV, erythrocyte and its nucleus areas compared with the control. Erythrocyte numbers, erythrocyte and its nucleus area increased at 2, 4 and 8 g L-1 concentrations of clinoptilolite during 7, 21 and 30 days exposure compared to 3 days exposure while hematocrit and MCV decreased. Erythrocyte numbers, erythrocyte and its nucleus area decreased at 32 g L-1 concentrations of clinoptilolite during 7, 21 and 30 days exposure compared to 3 days exposure while hematocrit and MCV increased (p<0.05).



Changes observed in hematocrit level and erythrocyte morphology might be due to the effect of studied concentrations of clinoptilolite on both gill and blood cell permeabilities.



Keywords

Cyprinus carpio clinoptilolite adsorbent blood

References

  • Agarwal, S., K. (2009). Heavy Metal Pollution. 270, New Delhi: APH Publishing.
  • Akkaya, C., Efeoğlu, A. & Yeşil, N. (2006). Avrupa Birliği su çerçeve direktifi ve Türkiye'de uygulanabilirliği. TMMOB Su Politikaları Kongresi Bildiri Kitabı 1. Cilt, 195-204.
  • Asgharimoghadam, A., Gharedaashi, E., Montajami, S., Nekoubin, H., Salamroudi, M. & Jafarian, H. (2012). Effect of clinoptilolite zeolite to prevent mortality of Beluga (Husohuso) by total ammonia concentration. Global Veterinaria, 9(1), 80-84. ISSN: 1992-6197
  • Bash, J., Berman, C. & Bolton, S. (2001). Effects of turbidity and suspended solids on salmonids. Center for Streamside Studies, University of Washington, Seattle, WA.
  • Becke, C., Steinhagen, D., Schumann, M. & Brinker, A. (2017). Physiological consequences for Rainbow Trout (Oncorhynchus mykiss) of Short-term exposure to increased suspended solid load. Aquacultural Engineering, 78(A), 63-74. DOI: 10.1016/j.aquaculture.2017.11.030
  • Çiftçi, N., Karayakar, F., Ay, Ö., Cicik, B. & Erdem, C. (2015). Effects of copper and lead on some hematological parameters of Oreochromis niloticus. Fresenius Environmental Bulletin, 24(9), 2771-2775.
  • Çiftçi, N., Özbay, T., Karayakar, F., Ay, Ö., Karaytuğ, S. & Cicik, B. (2013). Effects of Cadmium and Cadmium-Chitosan mixture on some blood parameters of Oreochromis niloticus. Journal of Biodiversity and Environmental Sciences, 7(21), 169-175.
  • Farhangi, M. & Rostami-Charati, F. (2012). Increasing of survival rate to Acipenser persicus by added clinoptilolite zeolite in acute toxicity test of ammonia. Aquaculture, Aquarium, Conservation& Legislation International Journal of the Bioflux Society, 5(1), 18-22.
  • Ghiasi, F. & Jasour, M.S. (2012). The effects of natural zeolite (Clinoptilolite) on water quality, growth performance and nutritional parameters of fresh water aquarium fish, Angel (Pterophyllum scalare). International Journal of Research in Fisheries and Aquaculture, 2(3), 22-25. ISSN: 2277-7729
  • Ghiasi, F. & Mirzargar, S.S. (2015). Pathological effects of cadmium and efficiency of natural zeolite, clinoptilolite, to reduce the cadmium toxicity in common carp and its ability to remove cadmium from contaminated water. Research Journal for Veterinary Practitioners, 3(2), 36-40. DOI: 10.14737/journal.rjvp/2015/3.2.36.40
  • Gisi, De, S., Lofrano, G., Grassi, M. & Notarnicola, M. (2016). Characteristics and adsorption capacities of low-cost sorbents for wastewater treatment: A review. Sustainable Materials and Technologies, 9, 10-40. DOI: 10.1016/j.susmat.2016.06.002
  • Gregory, T.R. (16 Ekim 2003). Fish erythrocyte sizes. (02 Şubat 2009) http://www.genomesize.com/cellsize/fish.html
  • Grimstone, A. V. & Skaer, R. J. (1972). A Quide book to microscopical methods. Cambridge University Press, 138.
  • Gülen, J., Zoray, F. & Arslan, S. (2012). Zeolitler ve kullanım alanları. Karaelmas Fen ve Mühendislik Dergisi, 2(1), 63-68.
  • Gürgün, V. & Halkman, A. K. (1988). Mikrobiyolojide sayım yöntemleri. 7, 146, Ankara: Gıda Teknolojisi Derneği. Heath, A. G. (1995). Water pollution and fish physiology. 4, 67-76, Virginia: Department of Biology Virginia Polytechnic Institute and State University Blacksburg.
  • Islam, Md., S. & Tanaka, M. (2004). Impacts of pollution on coastal and marine ecosystem, including coastal and marine fisheries and approach for management: A review and synthesis. Marine Pollution Bulletin, 48, 624-649. DOI: 10.1016/j.marpolbul.2003.12.004
  • James, R. & Sampath, K. (1999). Effect of zeolite on the reduction of cadmium toxicity in water and a freshwater fish, Oreochromis mossambicus. Bulletin of Environmental Contamination and Toxicology, 62, 222-229.
  • James, R., Sampath, K. & Selvamani, P. (1998). Effect of EDTA on reduction of copper toxicity in Oreochromis mossambicus (Peters). Bulletin of Environmental Contamination and Toxicology, 60(3), 487-93.
  • Konuk, T. (1981). Pratik Fizyoloji I. A.Ü. 181, Ankara Üniversitesi Basımevi, Ankara: Veteriner Fakültesi Yayınları. Marani, D., Macci, G. & Pagano, M. (1995). Lead precipitation in the presence of sulphate and carbonate: Testing of thermodynamic predictions. Water Research, 29(4), 1085-1092. DOI: 10.1016/0043-1354(94)00232-V
  • Mishra, M. & Jain, S.K. (2009). Effect of natural ion exchanger chabazite for remediation of lead toxicity: An experimental study in Teleost Fish Heteropneustes fossilis. Asian Journal of Experimental Sciences, 23(1), 39-44.
  • Papini, M.P., Kahie, Y.D., Troia, B. & Majone, M. (1999). Adsorption of lead at variable pH onto a natural porous medium: Modeling of batch and column experiments. Environmental Science and Technology, 33, 3357-4464. DOI: 10.1021/es990325j
  • Patterson, J. W. (1985). Industrial wastewater treatment technology, (2nd ed). Stoneham, M. A. Utterworths Publishers.
  • Priya, K. K., Ramesh, M., Saravanan, M. & Ponpandian, N. (2015). Ecological risk assessment of silicon dioxide nanoparticles in a freshwater fish Labeo rohita: hematology, ionoregulation and gill Na+/K+ATPase activity. Ecotoxicology and Environmental Safety, 120, 295–302. DOI: 10.1016/j.ecoenv.2015.05.032
  • Rubec, P. J. & Cruz, F. P.(2005). Monitoring the chain of custody to reduce delayed mortality of net-caught fish in the aquarium trade. SPC Live Reef Fish Information Bulletin, 13, 13-23.
  • Smith, S.R. (1996). Agricultural recycling of sewage sludge and the environment. CAB International,Wallingford, UK, 119-151.
  • Tepe, Y., Akyurt, I., Ciminli, C., Mutlu, E. & Çalışkan, M.(2004). Protective effect of clinoptilolite on lead toxicity in common carp Cyprinus carpio. Fresenius Environmental Bulletin, 13(7), 639-642.
  • Terzi, H. & Yıldız, M. (2011). Ağır metaller ve fitoremediasyon: fizyolojik ve moleküler mekanizmalar. Afyon Kocatepe Üniversitesi Fen Bilimleri Dergisi, 11, 1-22.
  • Tütüncü, S. İ. (2012). Üretimde kirlilik önleme yaklaşımı ve devletin konumu. Ekonomi Bilimleri Dergisi, 4(2), 119-129. ISSN: 1309-8020 (Online)
  • Wedemeyer, G. A., Barton, B., A. & Mcleay, D. J. (1990). Stress and Acclimation. In Methods for Fish Biology (Ed. by C. B. Shreck and P. B. Boyle), 451-489, Bethesda: American Fisheries Society.
  • Wedemeyer, G.A. & Yasutake, W.T. (1977). Clinical methods for the assessment of the effects of environmental stress on fish health. United States Technical Papers Fish Wild Services, 89, 1-18.

Doğal bir adsorban olan Klinoptilolitin, Cyprinus carpio ’nun bazı hematolojik parametreleri üzerine etkileri

Year 2018, Volume: 35 Issue: 4, 455 - 462, 15.12.2018

Okay Oğuztürk Nuray Çiftçi , Bedii Cicik

https://doi.org/10.12714/egejfas.2018.35.4.11

Abstract



Araştırmada, doğal bir şelatlayıcı ajan
olan klinoptilolitin (0,40 µ) 2, 4, 8, 16, 32 g/l’lik ortam derişimlerinin 3,
7, 21 ve 30 gün sürelerde, Cyprinus carpio’nun hematokrit düzeyi,
eritrosit sayısı, ortalama eritrosit hacmi (MCV), eritrosit ve eritrosit
nukleus alanı üzerine etkilerinin belirlenmesi amaçlanmıştır. İncelenen
parametrelerden hematokrit düzeyinin analizinde mikrohematokrit yöntemi,
eritrosit sayısı, MCV, eritrosit ve eritrosit nukleus alanının analizinde
mikroskobik yöntemler kullanılmıştır.



Eritrosit sayısı ve hematokrit
düzeyinin klinoptilolitin 16 ve 32 g/l ortam derişimlerinin 3 ve 7 günlük etki
süreleri dışında, belirlenen süre ve derişimlerinin etkisinde kontrole göre
düştüğü belirlenmiştir (p<0,05). MCV, eritrosit ve eritrosit nukleus
alanının klinoptilolitin etkisinde kontrole oranla artış gösterdiği
saptanmıştır. Klinoptilolitin 2, 4 ve 8 g/l derişimleri etkisinde eritrosit
sayısı, eritrosit ve eritrosit nükleus alanı 3. güne oranla 7, 21 ve 30 gün
etkisinde artarken, hematokrit düzeyi ile MCV düşmüştür. Klinoptilolitin 32 g/l
derişimi etkisinde ise eritrosit sayısı, eritrosit ve eritrosit nükleus alanı
3. güne oranla 7, 21 ve 30 gün sürelerde düşmüş, hematokrit düzeyi ile MCV
artmıştır (p<0,05).



Hematokrit düzeyi ile eritrosit
morfolojisinde meydana gelen değişimlerin klinoptilolitin uygulanan derişim ve
etki süresine bağlı olarak gerek solungaç gerekse kan hücrelerinin membran
permeabilitesindeki bozukluktan kaynaklanabileceği olasıdır. 



Keywords

Cyprinus carpio klinoptilolit adsorban kan

References

  • Agarwal, S., K. (2009). Heavy Metal Pollution. 270, New Delhi: APH Publishing.
  • Akkaya, C., Efeoğlu, A. & Yeşil, N. (2006). Avrupa Birliği su çerçeve direktifi ve Türkiye'de uygulanabilirliği. TMMOB Su Politikaları Kongresi Bildiri Kitabı 1. Cilt, 195-204.
  • Asgharimoghadam, A., Gharedaashi, E., Montajami, S., Nekoubin, H., Salamroudi, M. & Jafarian, H. (2012). Effect of clinoptilolite zeolite to prevent mortality of Beluga (Husohuso) by total ammonia concentration. Global Veterinaria, 9(1), 80-84. ISSN: 1992-6197
  • Bash, J., Berman, C. & Bolton, S. (2001). Effects of turbidity and suspended solids on salmonids. Center for Streamside Studies, University of Washington, Seattle, WA.
  • Becke, C., Steinhagen, D., Schumann, M. & Brinker, A. (2017). Physiological consequences for Rainbow Trout (Oncorhynchus mykiss) of Short-term exposure to increased suspended solid load. Aquacultural Engineering, 78(A), 63-74. DOI: 10.1016/j.aquaculture.2017.11.030
  • Çiftçi, N., Karayakar, F., Ay, Ö., Cicik, B. & Erdem, C. (2015). Effects of copper and lead on some hematological parameters of Oreochromis niloticus. Fresenius Environmental Bulletin, 24(9), 2771-2775.
  • Çiftçi, N., Özbay, T., Karayakar, F., Ay, Ö., Karaytuğ, S. & Cicik, B. (2013). Effects of Cadmium and Cadmium-Chitosan mixture on some blood parameters of Oreochromis niloticus. Journal of Biodiversity and Environmental Sciences, 7(21), 169-175.
  • Farhangi, M. & Rostami-Charati, F. (2012). Increasing of survival rate to Acipenser persicus by added clinoptilolite zeolite in acute toxicity test of ammonia. Aquaculture, Aquarium, Conservation& Legislation International Journal of the Bioflux Society, 5(1), 18-22.
  • Ghiasi, F. & Jasour, M.S. (2012). The effects of natural zeolite (Clinoptilolite) on water quality, growth performance and nutritional parameters of fresh water aquarium fish, Angel (Pterophyllum scalare). International Journal of Research in Fisheries and Aquaculture, 2(3), 22-25. ISSN: 2277-7729
  • Ghiasi, F. & Mirzargar, S.S. (2015). Pathological effects of cadmium and efficiency of natural zeolite, clinoptilolite, to reduce the cadmium toxicity in common carp and its ability to remove cadmium from contaminated water. Research Journal for Veterinary Practitioners, 3(2), 36-40. DOI: 10.14737/journal.rjvp/2015/3.2.36.40
  • Gisi, De, S., Lofrano, G., Grassi, M. & Notarnicola, M. (2016). Characteristics and adsorption capacities of low-cost sorbents for wastewater treatment: A review. Sustainable Materials and Technologies, 9, 10-40. DOI: 10.1016/j.susmat.2016.06.002
  • Gregory, T.R. (16 Ekim 2003). Fish erythrocyte sizes. (02 Şubat 2009) http://www.genomesize.com/cellsize/fish.html
  • Grimstone, A. V. & Skaer, R. J. (1972). A Quide book to microscopical methods. Cambridge University Press, 138.
  • Gülen, J., Zoray, F. & Arslan, S. (2012). Zeolitler ve kullanım alanları. Karaelmas Fen ve Mühendislik Dergisi, 2(1), 63-68.
  • Gürgün, V. & Halkman, A. K. (1988). Mikrobiyolojide sayım yöntemleri. 7, 146, Ankara: Gıda Teknolojisi Derneği. Heath, A. G. (1995). Water pollution and fish physiology. 4, 67-76, Virginia: Department of Biology Virginia Polytechnic Institute and State University Blacksburg.
  • Islam, Md., S. & Tanaka, M. (2004). Impacts of pollution on coastal and marine ecosystem, including coastal and marine fisheries and approach for management: A review and synthesis. Marine Pollution Bulletin, 48, 624-649. DOI: 10.1016/j.marpolbul.2003.12.004
  • James, R. & Sampath, K. (1999). Effect of zeolite on the reduction of cadmium toxicity in water and a freshwater fish, Oreochromis mossambicus. Bulletin of Environmental Contamination and Toxicology, 62, 222-229.
  • James, R., Sampath, K. & Selvamani, P. (1998). Effect of EDTA on reduction of copper toxicity in Oreochromis mossambicus (Peters). Bulletin of Environmental Contamination and Toxicology, 60(3), 487-93.
  • Konuk, T. (1981). Pratik Fizyoloji I. A.Ü. 181, Ankara Üniversitesi Basımevi, Ankara: Veteriner Fakültesi Yayınları. Marani, D., Macci, G. & Pagano, M. (1995). Lead precipitation in the presence of sulphate and carbonate: Testing of thermodynamic predictions. Water Research, 29(4), 1085-1092. DOI: 10.1016/0043-1354(94)00232-V
  • Mishra, M. & Jain, S.K. (2009). Effect of natural ion exchanger chabazite for remediation of lead toxicity: An experimental study in Teleost Fish Heteropneustes fossilis. Asian Journal of Experimental Sciences, 23(1), 39-44.
  • Papini, M.P., Kahie, Y.D., Troia, B. & Majone, M. (1999). Adsorption of lead at variable pH onto a natural porous medium: Modeling of batch and column experiments. Environmental Science and Technology, 33, 3357-4464. DOI: 10.1021/es990325j
  • Patterson, J. W. (1985). Industrial wastewater treatment technology, (2nd ed). Stoneham, M. A. Utterworths Publishers.
  • Priya, K. K., Ramesh, M., Saravanan, M. & Ponpandian, N. (2015). Ecological risk assessment of silicon dioxide nanoparticles in a freshwater fish Labeo rohita: hematology, ionoregulation and gill Na+/K+ATPase activity. Ecotoxicology and Environmental Safety, 120, 295–302. DOI: 10.1016/j.ecoenv.2015.05.032
  • Rubec, P. J. & Cruz, F. P.(2005). Monitoring the chain of custody to reduce delayed mortality of net-caught fish in the aquarium trade. SPC Live Reef Fish Information Bulletin, 13, 13-23.
  • Smith, S.R. (1996). Agricultural recycling of sewage sludge and the environment. CAB International,Wallingford, UK, 119-151.
  • Tepe, Y., Akyurt, I., Ciminli, C., Mutlu, E. & Çalışkan, M.(2004). Protective effect of clinoptilolite on lead toxicity in common carp Cyprinus carpio. Fresenius Environmental Bulletin, 13(7), 639-642.
  • Terzi, H. & Yıldız, M. (2011). Ağır metaller ve fitoremediasyon: fizyolojik ve moleküler mekanizmalar. Afyon Kocatepe Üniversitesi Fen Bilimleri Dergisi, 11, 1-22.
  • Tütüncü, S. İ. (2012). Üretimde kirlilik önleme yaklaşımı ve devletin konumu. Ekonomi Bilimleri Dergisi, 4(2), 119-129. ISSN: 1309-8020 (Online)
  • Wedemeyer, G. A., Barton, B., A. & Mcleay, D. J. (1990). Stress and Acclimation. In Methods for Fish Biology (Ed. by C. B. Shreck and P. B. Boyle), 451-489, Bethesda: American Fisheries Society.
  • Wedemeyer, G.A. & Yasutake, W.T. (1977). Clinical methods for the assessment of the effects of environmental stress on fish health. United States Technical Papers Fish Wild Services, 89, 1-18.
There are 30 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Okay Oğuztürk 0000-0001-6457-1775

Nuray Çiftçi 0000-0002-2925-0332

Bedii Cicik 0000-0003-3982-9943

Publication Date December 15, 2018
Submission Date May 16, 2018
Published in Issue Year 2018 Volume: 35 Issue: 4

Cite

APA Oğuztürk, O., Çiftçi, N., & Cicik, B. (2018). Effect of natural adsorbent Clinoptilolite on some hematological parameters of Cyprinus carpio. Ege Journal of Fisheries and Aquatic Sciences, 35(4), 455-462. https://doi.org/10.12714/egejfas.2018.35.4.11