Araştırma Makalesi
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Determination of Phytoplankton Pigment Concentration and Size Classes Using HPLC Technique (South-Eastern Black Sea-Rize)

Yıl 2016, Cilt: 16 Sayı: 2, 81 - 90, 22.02.2016

Ertuğrul Ağırbaş

https://doi.org/10.17693/yunusae.v16i21960.235785

Öz

In the present study, seasonal variations of phytoplankton and pigment composition from May 2014 to April 2015 were investigated along the south-eastern coasts (Rize) of the Black Sea. Surface (0,5 m) water samples were collected from five different stations (Iyidere, Rize, Cayeli, Pazar and Findikli station) during the study period. A total of 71species were identified during the study period, 41 species of these were dinoflagellate species, 18 species were diatoms and 12 species were other groups (silicoflagellate, euglenophyta, prymnesiophyta, chlorophyta etc.). In terms of species numbers, dinoflagellates contributed to 57,76% of the total phytoplankton species. Diatoms were the second important group with the contribution of 25,35% to the total phytoplankton species, and followed other groups with 16,89%. The dominant pigment in the area was chlorophyll-a (mean 1,57 μg/L) revealed a consistency with phytoplankton cell numbers. The second prominent pigment was fucoxanthin (mean 0,35 μg/L) and followed by 19-Hexanoyloxifucxanthin (mean 0,24 μg/L) and peridinin (mean 0,21 μg/L). The positive relationships between cell number and pigment concentrations revealed that these marker pigments can be used for taxonomic characterisation of phytoplankton groups.

Anahtar Kelimeler

Black Sea Phytoplankton Chlorophyll-a Fucoxanthin 19’-Hexanoyloxifucoxanthin

Kaynakça

  • Agirbas, E., Feyzioglu, A. M., Kopuz, U. ve Llewellyn, C. A. 2015. Phytoplankton community composition in the south-eastern Black Sea determined with pigments measured by HPLC-CHEMTAX analyses and microscopy cell counts. Journal of the Marine Biological Association of the United Kingdom, 95 (1), 35-52.
  • Ağırbaş, E. 2010. Güneydoğu Karadeniz’de Pigment Konsantrasyonu ve Birincil Üretimin Çevre Koşulları İle Etkileşimi, (Doktora Tezi) Karadeniz Teknik Üniversitesi, Sürmene Deniz Bilimleri Fakültesi, Trabzon.
  • Balech, E. 1988. Los dinoflaelados del Atlantici sudoccidental, Publ. Espec. Instituto Espanol Oceanografia, Madrid, 1: 299.
  • Bayraktar, S. 1994. Distribution of phytoplankton (>55 um) along Turkish Coast and at the North Western shelf area of the Black Sea (Yüksek Lisans Tezi), ODTÜ, 172 s.
  • Booth, B. C. 1993. Estimating Cell Concentration and Biomass of Autotrophic Plankton using Microscopy. In: Kemp, P.F., Sherr, B.F., Cole, J.J. (Eds.), Handbook of Methods in Aquatic Microbial Ecology. Lewis Publishers, Boca Raton, FL, 199-205.
  • Drebes, G. 1974. Marines phytoplankton, eine auswahl der Hegolaender planktonagen (Diatomeen, peridineen), Georg Thieme, Stuttgart, 286.
  • Ediger, D., Soydemir, N. ve Kideys, A. E. 2006. Estimation of Phytoplankton Biomas Using HPLC Pigment Analysis in the Southwestern Black Sea, Deep-Sea Research Part II, 53, 1911-1922.
  • Eker-Develi, E., Berthon, J. F., Canuti, E., Slabakova, N., Moncheva, S., Shtereva, G. ve Dzhurova, B. 2012. Phytoplankton taxonomy based on CHEMTAX and microscopy in the Northwestern Black Sea. Journal of Marine Systems 94, 18–32.
  • Eker-Develi, E., Berthon, J. F. ve Linde, D. 2008. Phytoplankton class determination by microscopic and HPLC-CHEMTAX analyses in the southern Baltic Sea. Marine Ecology Progress Series 359, 69–87.
  • Eker, E., Georgieva, L., Senichkina, L. ve Kideys, A. E. 1999. Phytoplankton Distribution in the Western and Eastern Black Sea in Spring and Autumn1995, ICES Journal of Marine Science,56, 15–22.
  • Falciatore, A., Ribera, d'Alcalà, M., Croot, P. ve Bowler, C. 2000. Perception of Environmental Signals by a Marine Diatom, Science 288 (5475), 2363-2366.
  • Fukuyo, Y., Takano, H., Chihara, M. ve Matsuoka, K. 1990. Red tide organizms in Japon (An illustrated taxonomic guide), Uchida Rokakuho Publishing, Tokyo, 407.
  • Hay, B., Honjo, S., Kempe, S., Itekkot, V. A., Degens E. T., Konuk, T. ve İzdar, E. 1990. Interannual Variability in Particle flux in the Southwestern Black Sea, Deep-Sea Research, 37, 911-928.
  • Honjo, S., Hay, B., Manganini, S. J., Degens, E. T., Kempe, S., Ittekkot, V. A., Izdar, E., Konuk ,T. ve Benli, H. A. 1987. Seasonal Cyclicity of Lithogenic Particle Fluxes at a Southern Black Sea Sediment Trap Station, Mitt. Geol.-Palaont. Inst., University of Hamburg, F.R.G., 19-39.
  • Koca, L. 2014. Güney Doğu Karadeniz Kıyıları (Rize) Diatom/Dinoflagellat Oranları ve Pigment Kompozisyonun Zamansal Değişimi, (Yüksek Lisans Tezi) Recep Tayyip Erdoğan Üniversitesi, Fen Bilimleri Enstitüsü, Trabzon.
  • Mackey M. D., Mackey, D. J., Higgins, H. W. ve Wright, S. W. 1996. CHEMTAX—a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Marine Ecology Progress Series 144, 265–283.
  • Mantoura, R. F. C. ve Llewellyn, C. A. 1983. The rapid determination of algal Chlorophyll-a and Carotenoid Pigments and Their Breakdown Products in Natural Waters by Reverse-Phase High Performance Liquid Chromatography, Analytica Chimica Acta, 151, 297-314.
  • Nagata, T., Takai, K., Kawabata, K. I., Nakanishi, M. ve Urabe, J. 1996. The trophic transfer via a picoplankton-flagellate-copepod food chain during a picocyanobacterial bloom in Lake Biwa. Arch Hydrobiol 137:145–160.
  • Nelson, D. M. D., Trrguer, P., Brzezinski, M. A., Leynaert, A., Queguiner, B. ve Tréguer, P. 1995. Production and dissolution of biogenic silica in the ocean: revised global estimates, comparison with regional data and relationship to biogenic sedimentation. Glob Biogeochemical Cycle 9: 359-372.
  • Ramphi, L. ve Bernhard, M. 1978. Key for the determination of Mediterranean pelagic diatoms, Comit. Naz. Energia Nucleare, Roma, 72.
  • Sorokin, Y. U. 1986. The Black Sea in: Ecosystem of the World, 26 Estaures and Enclosed Seas, Elsevier Publishing, New York, 253-292.
  • Spector, D. L. 1984. Dinoflagellates, Academic Press, Florida, 545.
  • Sur, H. I., Özsoy, E., Ilyin, Y. P. ve Ünlüata, U. 1996. Coastal/Deep Ocean Interactions in the Black Sea and their Ecological/Environmental Impacts, Journal of Marine Systems, 7, 293-320.
  • Tait, R. V. ve Dipper, F. A. 2001. Elements of Marine Ecology, Butterworth-Heinemann Pub., forth edition, Gread Britaiin, 462.
  • Tomas, C. R. 1993. Marine Phytoplankton, A Guide to Naked Flagellates and Coccolithophotids, Academic Press, London, 263.
  • Tomas, C. R. 1996. Identification Marine Diatoms and Dinoflagellates, Academic Press, San Diego, 598.
  • Uitz, J., Claustre, H., Morel, A. ve Hooker, S. B. 2006. Vertical distribution of phytoplankton communities in open ocean: An assessment based on surface chlorophyll. Journal of Geophysical Research-Oceans 111 (C8).
  • Utermohl, H. 1958. Zur Vervollkommnung der quantitativen phytoplankton: Methodik Mitteilung Internationale Vereinigung Theoretische und Angewandte Limnologie, 9, 1–38.
  • Vedernikov, V. I. ve Demidov, A. B. 1993. Primary production and chlorophyll in the deep regions of the Black Sea. Oceanology 33, 229–235.
  • Verity, P. G. ve Smetacek, V. 1996. Organism life cycles, predation, and the structure of marine pelagic systems. Marine Ecology Progress Series 130:277–293
  • Vidussi, F., Claustre, H., Manca, B. B., Luchetta, A. ve Marty, J. C. 2001. Phytoplankton pigment distribution in relation to upper thermocline circulation in the eastern Mediterranean Sea during winter. J. Geophys. Res. Oceans 106 (C9), 19939–19956.
  • Yayla, M., Yılmaz, A. ve Morkoç, E. 2001. The Dynamics of Nutrient Enrichment and Primary Production Related to Recent Changes in the Ecosystem of the Black Sea, Aquatic Ecosystem Health and Management, 4, 33-49.
  • Yılmaz, A., Tuğrul, S., Polat, Ç., Ediger, D., Çoban, Y. ve Morokoç, E. 1998. On the Production, Elemental Composition (C, N, P) and Distribution of Photosynthetic Organic Matter in the Southern Black Sea, Hydrobiologia, 363,141-156.
  • Yunev, O., Vladimir, A., Baştürk, Ö., Yılmaz, A., Kideyş, A. E., Moncheva, S. ve Konovalov, S. K. 2002. Long-term Variation of Surface Chlorophyll-a and Primary Production in the open Black Sea, Marine Ecology Progress Series, 230, 11-28.
  • Zaitsev, Y. P. 1997. Eutrophication of the Black Sea and Its Major Consequences, Black Sea Pollution Assesment, Black Sea Environmental Series.

Fitoplankton Pigment Konsantrasyonunun ve Boy Gruplarının HPLC Tekniği ile Belirlenmesi (Güneydoğu Karadeniz, Rize)

Yıl 2016, Cilt: 16 Sayı: 2, 81 - 90, 22.02.2016

Ertuğrul Ağırbaş

https://doi.org/10.17693/yunusae.v16i21960.235785

Öz

Mayıs 2014-Nisan 2015 tarihleri arasında Güney Doğu Karadeniz kıyılarında (Rize) yürütülen bu çalışmada, fitoplankton ve pigment konsantrasyonunun mevsimsel değişimleri araştırılmıştır. Araştırma süresince beş farklı istasyonda (İyidere, Rize, Çayeli, Pazar ve Fındıklı istasyonu) yüzey suyundan (0,5 m) örneklemeler yapılmıştır. Çalışmada, dinoflagellatlar tür sayısı bakımdan en fazla gözlenen fitoplankton grubu olmuştur. Çalışma dönemi boyunca toplam 71 tür belirlenmiş, bunların 41’i dinoflagellat, 18’i diatom ve 12’si de diğer gruplar (silikoflagellata, öglenofita, primnesiofita, klorofita vb.) altında değerlendirilmiştir. Kalitatif olarak toplam fitoplankton tür sayısında grupların yaptığı katkı değerlendirildiğine dinoflagellat %57,76 ile ilk sırada yer alırken, bunu %25,35 ile diatom türleri ve %16,89 ile diğer gruplara ait türler oluşturmuşlardır. Bölgede baskın pigment klorofil-a olup (ortalama 1,57 μg/L) fitoplankton hücre sayısı ile uyum içerisinde olduğu tespit edilmiştir. Klorofil-a’dan sonra en baskın pigment fukoksantin olup (ortalama 0,35 μg/L) bunu sırasıyla 19’-Heksanoloksifukoksantin (ortalama 0,24 μg/L) ve peridinin pigmenti (ortalama 0,21 μg/L) takip etmiştir. HPLC sonuçlarına göre pigment konsantrasyonları ile hücre sayıları arasında tespit edilen pozitif ilişkiler marker pigmentlerin fitoplankton gruplarını taksonomik açıdan karakterize etmek için kullanılabileceğini göstermiştir.


Anahtar Kelimeler

Karadeniz Fitoplankton Klorofil-a Peridinin Fukoksantin 19’- Heksanoloksifukoksantin

Kaynakça

  • Agirbas, E., Feyzioglu, A. M., Kopuz, U. ve Llewellyn, C. A. 2015. Phytoplankton community composition in the south-eastern Black Sea determined with pigments measured by HPLC-CHEMTAX analyses and microscopy cell counts. Journal of the Marine Biological Association of the United Kingdom, 95 (1), 35-52.
  • Ağırbaş, E. 2010. Güneydoğu Karadeniz’de Pigment Konsantrasyonu ve Birincil Üretimin Çevre Koşulları İle Etkileşimi, (Doktora Tezi) Karadeniz Teknik Üniversitesi, Sürmene Deniz Bilimleri Fakültesi, Trabzon.
  • Balech, E. 1988. Los dinoflaelados del Atlantici sudoccidental, Publ. Espec. Instituto Espanol Oceanografia, Madrid, 1: 299.
  • Bayraktar, S. 1994. Distribution of phytoplankton (>55 um) along Turkish Coast and at the North Western shelf area of the Black Sea (Yüksek Lisans Tezi), ODTÜ, 172 s.
  • Booth, B. C. 1993. Estimating Cell Concentration and Biomass of Autotrophic Plankton using Microscopy. In: Kemp, P.F., Sherr, B.F., Cole, J.J. (Eds.), Handbook of Methods in Aquatic Microbial Ecology. Lewis Publishers, Boca Raton, FL, 199-205.
  • Drebes, G. 1974. Marines phytoplankton, eine auswahl der Hegolaender planktonagen (Diatomeen, peridineen), Georg Thieme, Stuttgart, 286.
  • Ediger, D., Soydemir, N. ve Kideys, A. E. 2006. Estimation of Phytoplankton Biomas Using HPLC Pigment Analysis in the Southwestern Black Sea, Deep-Sea Research Part II, 53, 1911-1922.
  • Eker-Develi, E., Berthon, J. F., Canuti, E., Slabakova, N., Moncheva, S., Shtereva, G. ve Dzhurova, B. 2012. Phytoplankton taxonomy based on CHEMTAX and microscopy in the Northwestern Black Sea. Journal of Marine Systems 94, 18–32.
  • Eker-Develi, E., Berthon, J. F. ve Linde, D. 2008. Phytoplankton class determination by microscopic and HPLC-CHEMTAX analyses in the southern Baltic Sea. Marine Ecology Progress Series 359, 69–87.
  • Eker, E., Georgieva, L., Senichkina, L. ve Kideys, A. E. 1999. Phytoplankton Distribution in the Western and Eastern Black Sea in Spring and Autumn1995, ICES Journal of Marine Science,56, 15–22.
  • Falciatore, A., Ribera, d'Alcalà, M., Croot, P. ve Bowler, C. 2000. Perception of Environmental Signals by a Marine Diatom, Science 288 (5475), 2363-2366.
  • Fukuyo, Y., Takano, H., Chihara, M. ve Matsuoka, K. 1990. Red tide organizms in Japon (An illustrated taxonomic guide), Uchida Rokakuho Publishing, Tokyo, 407.
  • Hay, B., Honjo, S., Kempe, S., Itekkot, V. A., Degens E. T., Konuk, T. ve İzdar, E. 1990. Interannual Variability in Particle flux in the Southwestern Black Sea, Deep-Sea Research, 37, 911-928.
  • Honjo, S., Hay, B., Manganini, S. J., Degens, E. T., Kempe, S., Ittekkot, V. A., Izdar, E., Konuk ,T. ve Benli, H. A. 1987. Seasonal Cyclicity of Lithogenic Particle Fluxes at a Southern Black Sea Sediment Trap Station, Mitt. Geol.-Palaont. Inst., University of Hamburg, F.R.G., 19-39.
  • Koca, L. 2014. Güney Doğu Karadeniz Kıyıları (Rize) Diatom/Dinoflagellat Oranları ve Pigment Kompozisyonun Zamansal Değişimi, (Yüksek Lisans Tezi) Recep Tayyip Erdoğan Üniversitesi, Fen Bilimleri Enstitüsü, Trabzon.
  • Mackey M. D., Mackey, D. J., Higgins, H. W. ve Wright, S. W. 1996. CHEMTAX—a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Marine Ecology Progress Series 144, 265–283.
  • Mantoura, R. F. C. ve Llewellyn, C. A. 1983. The rapid determination of algal Chlorophyll-a and Carotenoid Pigments and Their Breakdown Products in Natural Waters by Reverse-Phase High Performance Liquid Chromatography, Analytica Chimica Acta, 151, 297-314.
  • Nagata, T., Takai, K., Kawabata, K. I., Nakanishi, M. ve Urabe, J. 1996. The trophic transfer via a picoplankton-flagellate-copepod food chain during a picocyanobacterial bloom in Lake Biwa. Arch Hydrobiol 137:145–160.
  • Nelson, D. M. D., Trrguer, P., Brzezinski, M. A., Leynaert, A., Queguiner, B. ve Tréguer, P. 1995. Production and dissolution of biogenic silica in the ocean: revised global estimates, comparison with regional data and relationship to biogenic sedimentation. Glob Biogeochemical Cycle 9: 359-372.
  • Ramphi, L. ve Bernhard, M. 1978. Key for the determination of Mediterranean pelagic diatoms, Comit. Naz. Energia Nucleare, Roma, 72.
  • Sorokin, Y. U. 1986. The Black Sea in: Ecosystem of the World, 26 Estaures and Enclosed Seas, Elsevier Publishing, New York, 253-292.
  • Spector, D. L. 1984. Dinoflagellates, Academic Press, Florida, 545.
  • Sur, H. I., Özsoy, E., Ilyin, Y. P. ve Ünlüata, U. 1996. Coastal/Deep Ocean Interactions in the Black Sea and their Ecological/Environmental Impacts, Journal of Marine Systems, 7, 293-320.
  • Tait, R. V. ve Dipper, F. A. 2001. Elements of Marine Ecology, Butterworth-Heinemann Pub., forth edition, Gread Britaiin, 462.
  • Tomas, C. R. 1993. Marine Phytoplankton, A Guide to Naked Flagellates and Coccolithophotids, Academic Press, London, 263.
  • Tomas, C. R. 1996. Identification Marine Diatoms and Dinoflagellates, Academic Press, San Diego, 598.
  • Uitz, J., Claustre, H., Morel, A. ve Hooker, S. B. 2006. Vertical distribution of phytoplankton communities in open ocean: An assessment based on surface chlorophyll. Journal of Geophysical Research-Oceans 111 (C8).
  • Utermohl, H. 1958. Zur Vervollkommnung der quantitativen phytoplankton: Methodik Mitteilung Internationale Vereinigung Theoretische und Angewandte Limnologie, 9, 1–38.
  • Vedernikov, V. I. ve Demidov, A. B. 1993. Primary production and chlorophyll in the deep regions of the Black Sea. Oceanology 33, 229–235.
  • Verity, P. G. ve Smetacek, V. 1996. Organism life cycles, predation, and the structure of marine pelagic systems. Marine Ecology Progress Series 130:277–293
  • Vidussi, F., Claustre, H., Manca, B. B., Luchetta, A. ve Marty, J. C. 2001. Phytoplankton pigment distribution in relation to upper thermocline circulation in the eastern Mediterranean Sea during winter. J. Geophys. Res. Oceans 106 (C9), 19939–19956.
  • Yayla, M., Yılmaz, A. ve Morkoç, E. 2001. The Dynamics of Nutrient Enrichment and Primary Production Related to Recent Changes in the Ecosystem of the Black Sea, Aquatic Ecosystem Health and Management, 4, 33-49.
  • Yılmaz, A., Tuğrul, S., Polat, Ç., Ediger, D., Çoban, Y. ve Morokoç, E. 1998. On the Production, Elemental Composition (C, N, P) and Distribution of Photosynthetic Organic Matter in the Southern Black Sea, Hydrobiologia, 363,141-156.
  • Yunev, O., Vladimir, A., Baştürk, Ö., Yılmaz, A., Kideyş, A. E., Moncheva, S. ve Konovalov, S. K. 2002. Long-term Variation of Surface Chlorophyll-a and Primary Production in the open Black Sea, Marine Ecology Progress Series, 230, 11-28.
  • Zaitsev, Y. P. 1997. Eutrophication of the Black Sea and Its Major Consequences, Black Sea Pollution Assesment, Black Sea Environmental Series.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makalesi
Yazarlar

Ertuğrul Ağırbaş

Yayımlanma Tarihi 22 Şubat 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 16 Sayı: 2

Kaynak Göster

APA Ağırbaş, E. (2016). Fitoplankton Pigment Konsantrasyonunun ve Boy Gruplarının HPLC Tekniği ile Belirlenmesi (Güneydoğu Karadeniz, Rize). Aquaculture Studies, 16(2), 81-90. https://doi.org/10.17693/yunusae.v16i21960.235785
AMA Ağırbaş E. Fitoplankton Pigment Konsantrasyonunun ve Boy Gruplarının HPLC Tekniği ile Belirlenmesi (Güneydoğu Karadeniz, Rize). AquaST. Mart 2016;16(2):81-90. doi:10.17693/yunusae.v16i21960.235785
Chicago Ağırbaş, Ertuğrul. “Fitoplankton Pigment Konsantrasyonunun Ve Boy Gruplarının HPLC Tekniği Ile Belirlenmesi (Güneydoğu Karadeniz, Rize)”. Aquaculture Studies 16, sy. 2 (Mart 2016): 81-90. https://doi.org/10.17693/yunusae.v16i21960.235785.
EndNote Ağırbaş E (01 Mart 2016) Fitoplankton Pigment Konsantrasyonunun ve Boy Gruplarının HPLC Tekniği ile Belirlenmesi (Güneydoğu Karadeniz, Rize). Aquaculture Studies 16 2 81–90.
IEEE E. Ağırbaş, “Fitoplankton Pigment Konsantrasyonunun ve Boy Gruplarının HPLC Tekniği ile Belirlenmesi (Güneydoğu Karadeniz, Rize)”, AquaST, c. 16, sy. 2, ss. 81–90, 2016, doi: 10.17693/yunusae.v16i21960.235785.
ISNAD Ağırbaş, Ertuğrul. “Fitoplankton Pigment Konsantrasyonunun Ve Boy Gruplarının HPLC Tekniği Ile Belirlenmesi (Güneydoğu Karadeniz, Rize)”. Aquaculture Studies 16/2 (Mart 2016), 81-90. https://doi.org/10.17693/yunusae.v16i21960.235785.
JAMA Ağırbaş E. Fitoplankton Pigment Konsantrasyonunun ve Boy Gruplarının HPLC Tekniği ile Belirlenmesi (Güneydoğu Karadeniz, Rize). AquaST. 2016;16:81–90.
MLA Ağırbaş, Ertuğrul. “Fitoplankton Pigment Konsantrasyonunun Ve Boy Gruplarının HPLC Tekniği Ile Belirlenmesi (Güneydoğu Karadeniz, Rize)”. Aquaculture Studies, c. 16, sy. 2, 2016, ss. 81-90, doi:10.17693/yunusae.v16i21960.235785.
Vancouver Ağırbaş E. Fitoplankton Pigment Konsantrasyonunun ve Boy Gruplarının HPLC Tekniği ile Belirlenmesi (Güneydoğu Karadeniz, Rize). AquaST. 2016;16(2):81-90.