Trails for immunization of rainbow trout (Oncorhynchus mykiss) against to white spot disease (Ichthyophthirius multifiliis)
Öz
In this study, the
humoral immune responses and host protection of rainbow trout, O. mykiss (Walbaum, 1792) against
Ichthyophthirius multifiliis (Ich)
were determined after vaccination with live theronts, sonicated trophonts,
adjuvant added heat inactived theronts. Immunizations with live theronts,
sonicated trophonts or adjuvant added heat inactive theronts by intraperitoneal
(I.P.) injection. Serum immobilization titres were determined 21 days
post-immunization. Four weeks after immunization, the number of trophonts were assessed in rainbow
trout skin four days after challenge. Mean of serum immobilization titres
levels were significantly higher (P<0.05) in fish immunized with live
theronts (1/67,4) and sonicated trophonts (1/29,7) by I.P. injection, than in
the fish immunized with adjuvant added heat inactivated theronts (1/11,4) and
PBS (1/9,42) by I.P. injection. Mean number of the trophonts that can
penetrated to skins were significantly lower (P<0.05) in fish administered
live theronts (21 trophonts/fish) and sonicated trophonts (29,13 tr/fish) by
i.p. injection, than in the fish immunized with adjuvant added heat inactivated
theronts (48,53 tr/fish) and PBS (54,07 tr/fish) by I.P. injection. Therefore, partial
protection was noted on those fish. There was found positive correlation
between higher levels of immobilization titres and partial protection in the
immunized fish.
Anahtar Kelimeler
Pelophylax bedriagae Levant Water Frog Lake Sülüklü breeding ecology population parameters
Kaynakça
- Alishahi, M., & Buchmann, K. (2006). Temperature-dependent protection against Ichthyophthirius multifiliis fallowing immunisation of rainbow trout using live theronts. Diseases of Aquatic Organisms, 72: 269-273.
- Buchmann, K., Lindenstrom, T., & Sigh, J. (1999). Partial cross protection against Ichthyophthirius multifiliis in Gyrodactylus derjavini immunized rainbow trout. Journal of Helminthology, 73: 189-195.
- Burkart, M.A., Clark, T.G., & Dickerson, H.W. (1990). Immunization of channel catfish, Ictalurus punctatus Rafinesque, against Ichthyophthirius multifiliis (Fouquet): killed versus live vaccines. Journal of Fish Diseases, 13: 401-410.
- Dalgaard, M., Buchmann, K., & Li, A. (2002). Immunization of rainbow trout fry with Ichthyophthirius multifiliis sonicate: protection of host and immunological changes. Bulletin of the European Association of Fish Pathologists, 22(5): 288-296.
- Dickerson, H.W., & Clark, T.G. (1996). Immune response of fishes to ciliates. Annual Review of Fish Diseases, 6: 107-120.
- Dickerson, H.W., & Dawe, D.L. (1995). Ichthyophthirius multifiliis and Cryptocaryon irritans in Woo, P.T.K. Fish Disease and Disorders, Vol. 1, Cab International, Cambridge, 789 p.
- FAO (2014), ftp://ftp.fao.org/FI/STAT/summary/default.htm (10.10.2016)
- Gleeson, D.J., Mccallum, H.I., & Owens, I.P. (2000). Differences in initial and acquired resistance to Ichthyophthirius multifiliis between populations of rainbowfish. Journal of Fish Biology 57: 466-475.
- Hines, R.S., & Spira, D.T. (1974). Ichthyophthiriasis in mirror carp Cyprinus carpio (l.) V. acquired immunity. Journal of Fish Biology, 6: 373-378.
- Houghton, G., & Matthews, R.A. (1993). Ichthyophthirius multifiliis (Fouquet): survival within immune juvenile carp, Cyprinus carpio L. Fish and Shellfish Immunology, 3: 157-166.
- Jørgensen, L.V.G., Nemli, E., Heinecke, R.D., Raida, M.K., & Buchmann, K. (2008). Immune-relevant genes expressed in rainbow trout following immunisation with a live vaccine against Ichthyophthirius multifiliis. Disaeses of Aquatic Organisms, 80: 189-197.
- Kochva, L. (1962). Parasites of Freshwater Fish and The Biological Basis for Their Control. Sivan Press, Jerusalem, 236 p.
- Lom, J., & Dykova, I. (1992). Protozoan parasites of fishes. Elsevier Science Publishers B.V., Amsterdam, 315 p.
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951). Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem.193:265-275
- Osman, H.A.M., Monier, M.M., Abd El Ghany, O.A., Ibrahim, T.G., & Ismail, M.M. (2009). Protection of goldfish (Carassius auratus) against Ichthyophthirius multifiliis by immunization with live theronts, trophonts and sonicated trophonts. Global Veterineria, 3(4): 329-334.
- Shinn, A.P., Wootten, R., & Sommerville, C., (2003). Alternative compounds for thetreatment of Ichthyophtirius multifiliis infecting rainbow trout. Trout News, 35: 38-41.
- Sigh, J., (2003). The immune response ın rainbow trout (oncorhynchus mykiss) against the ciliate Ichthyophthirius multifiliis fouquet, 1876 selected immune mechanisms and testing of dna vaccination. The Royal Veterinary and Agricultural University, Denmark. (PhD Thesis) 120p.
- Sigh, J., & Buchmann, K. (2001). Comparision of immobilization assays and enzyme-linked immunsorbent assays for detection of rainbow trout antibody-titres against Ichthyophthirius multifiliis. Fouquet, 1876, Journal of Fish Diseases, 24: 49-51.
- Sigh, J., & Buchmann, K. (2002). Comparative analysis of cross-reactivity between Ichthyophthirius and Tetrahymena. Bulletin of the European Association of Fish Pathologists, 22: 37-44.
- TUIK (2015). http://www.tuik.gov.tr/PreHaberBultenleri.do?id=21720 (05.01.2017)
- Vagsholm, I., & Djupvik, H.O. (1999). Risk factors for abdominal adhesions in atlantic salmon, Salmo salar L. Journal of Fish Diseases, 22, 1, 53-58.
- Wang, X., & Dickerson, H.W. (2002). Surface Immobilization Antigen of the Parasitic Ciliate Ichthyophthirius multifiliis Elicits Protective Immunity in Channel Catfish (Ictalurus punctatus). Clinical and Diagnostic Laboratory Immunology, 9: 176-181.
- Xu, D.H., & Klesius P.H. (2002). Antibody mediated immune response against Ichthyophthirius multifiliis using excised skin from channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius. Journal of Fish Diseases, 25: 299–306.
- Xu, D.H., & Klesius P.H. (2003). Protective effect of cutaneous antibody produced by channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius multifiliis Fouquet on cohabited non-immune catfish. Journal of Fish Diseases, 26: 287-291.
- Xu, D.H., Klesius, P.H., & Shelby, R.A. (2004). Immune responses and host protection of channelcatfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis after immunization with live theronts and sonicated trophonts. Diseases of Aquatic Organisms, 27: 135-141.
- Xu, D.H., Klesius, P.H., & Shoemaker, C.A. (2005). Cutaneous antibodies from channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius multifiliis (Ich) may induce apoptosis of Ich theronts. Journal of Fish Diseases, 28: 213-220.
- Xu, D.H., Klesius P.H., & Panangala, V.S. (2006). Induced cross-protection in channel catfish, Ictalurus punctatus (Rafinesque), against different immobilization serotypes of Ichthyophthirius multifiliis. Journal of Fish Diseases, 29: 131-138.
- Xu, D.H., Klesius, P.H. & Shoemaker, C.A. (2008). Protective immunity of Nile tilapia against Ichthyophthirius multifiliis post-immunization with live theronts and sonicated trophonts. Fish and Shellfish Immunology, 25: 124-127.
Gökkuşağı alabalıkları (Oncorhynchus mykiss)’nda beyaz benek hastalığına (Ichthyophthirius multifiliis) karşı bağışıklık kazandırılması
Öz
Bu çalışmada canlı teront, sonike trofont, ısı ile inaktive
adjuvantlı teront ile aşılanan gökkuşağı alabalıklarında O. mykiss (Walbaum,
1792), Ichthyophthirius multifiliis’e (Ich) karşı oluşan humoral immun
yanıt belirlenmiştir. Canlı teront, sonike trofont, ısı ile inaktive adjuvantlı
teront kullanılarak intraperitonal (İ.P.) enjeksiyon yoluyla immunizasyon
gerçekleştirildi. İmmunizasyondan 21 gün sonra serum immobilizasyon titreleri
belirlendi. Dört hafta sonra yapılan deneysel enfeksiyondan 4 gün sonra deriye
tutunan trofont sayısı belirlendi. İ.P. yolla canlı teront (1/67,4) ve sonike
trofontla (1/29,7) immunize edilen
balıkların, ortalama serum anti-Ich immobilizanyon antikor seviyeleri, ısı ile
inaktive adjuvantlı teront (1/11,4) ve PBS (1/9,4) enjeksiyonuyla immunize
edilenlere göre daha yüksek (P<0.05) bulundu. Deneysel enfeksiyon sonrası,
İ.P. yolla canlı teront (54,07 trofont/balık) ve sonike torofont (48,53
tr/balık) uygulanan balıklarda, ısı ile inaktive adjuvantlı teront (29,13
tr/balık) ve P.B.S (21 tr/balık) enjekte edilenlere göre, deriye penetre
olabilen ortalama trofont sayısı önemli derecede (P<0.05) azaldığından
dolayı bu balıklarda kısmi korunma meydana geldiği belirlendi. İmmobilizasyon
titresindeki artış ile immun balıklardaki kısmi korunma arasında pozitif
korelasyon bulundu.
Anahtar Kelimeler
Ichthyophthirius multifiliis aşılama gökkuşağı alabalığı immobilizasyon titresi
Kaynakça
- Alishahi, M., & Buchmann, K. (2006). Temperature-dependent protection against Ichthyophthirius multifiliis fallowing immunisation of rainbow trout using live theronts. Diseases of Aquatic Organisms, 72: 269-273.
- Buchmann, K., Lindenstrom, T., & Sigh, J. (1999). Partial cross protection against Ichthyophthirius multifiliis in Gyrodactylus derjavini immunized rainbow trout. Journal of Helminthology, 73: 189-195.
- Burkart, M.A., Clark, T.G., & Dickerson, H.W. (1990). Immunization of channel catfish, Ictalurus punctatus Rafinesque, against Ichthyophthirius multifiliis (Fouquet): killed versus live vaccines. Journal of Fish Diseases, 13: 401-410.
- Dalgaard, M., Buchmann, K., & Li, A. (2002). Immunization of rainbow trout fry with Ichthyophthirius multifiliis sonicate: protection of host and immunological changes. Bulletin of the European Association of Fish Pathologists, 22(5): 288-296.
- Dickerson, H.W., & Clark, T.G. (1996). Immune response of fishes to ciliates. Annual Review of Fish Diseases, 6: 107-120.
- Dickerson, H.W., & Dawe, D.L. (1995). Ichthyophthirius multifiliis and Cryptocaryon irritans in Woo, P.T.K. Fish Disease and Disorders, Vol. 1, Cab International, Cambridge, 789 p.
- FAO (2014), ftp://ftp.fao.org/FI/STAT/summary/default.htm (10.10.2016)
- Gleeson, D.J., Mccallum, H.I., & Owens, I.P. (2000). Differences in initial and acquired resistance to Ichthyophthirius multifiliis between populations of rainbowfish. Journal of Fish Biology 57: 466-475.
- Hines, R.S., & Spira, D.T. (1974). Ichthyophthiriasis in mirror carp Cyprinus carpio (l.) V. acquired immunity. Journal of Fish Biology, 6: 373-378.
- Houghton, G., & Matthews, R.A. (1993). Ichthyophthirius multifiliis (Fouquet): survival within immune juvenile carp, Cyprinus carpio L. Fish and Shellfish Immunology, 3: 157-166.
- Jørgensen, L.V.G., Nemli, E., Heinecke, R.D., Raida, M.K., & Buchmann, K. (2008). Immune-relevant genes expressed in rainbow trout following immunisation with a live vaccine against Ichthyophthirius multifiliis. Disaeses of Aquatic Organisms, 80: 189-197.
- Kochva, L. (1962). Parasites of Freshwater Fish and The Biological Basis for Their Control. Sivan Press, Jerusalem, 236 p.
- Lom, J., & Dykova, I. (1992). Protozoan parasites of fishes. Elsevier Science Publishers B.V., Amsterdam, 315 p.
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951). Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem.193:265-275
- Osman, H.A.M., Monier, M.M., Abd El Ghany, O.A., Ibrahim, T.G., & Ismail, M.M. (2009). Protection of goldfish (Carassius auratus) against Ichthyophthirius multifiliis by immunization with live theronts, trophonts and sonicated trophonts. Global Veterineria, 3(4): 329-334.
- Shinn, A.P., Wootten, R., & Sommerville, C., (2003). Alternative compounds for thetreatment of Ichthyophtirius multifiliis infecting rainbow trout. Trout News, 35: 38-41.
- Sigh, J., (2003). The immune response ın rainbow trout (oncorhynchus mykiss) against the ciliate Ichthyophthirius multifiliis fouquet, 1876 selected immune mechanisms and testing of dna vaccination. The Royal Veterinary and Agricultural University, Denmark. (PhD Thesis) 120p.
- Sigh, J., & Buchmann, K. (2001). Comparision of immobilization assays and enzyme-linked immunsorbent assays for detection of rainbow trout antibody-titres against Ichthyophthirius multifiliis. Fouquet, 1876, Journal of Fish Diseases, 24: 49-51.
- Sigh, J., & Buchmann, K. (2002). Comparative analysis of cross-reactivity between Ichthyophthirius and Tetrahymena. Bulletin of the European Association of Fish Pathologists, 22: 37-44.
- TUIK (2015). http://www.tuik.gov.tr/PreHaberBultenleri.do?id=21720 (05.01.2017)
- Vagsholm, I., & Djupvik, H.O. (1999). Risk factors for abdominal adhesions in atlantic salmon, Salmo salar L. Journal of Fish Diseases, 22, 1, 53-58.
- Wang, X., & Dickerson, H.W. (2002). Surface Immobilization Antigen of the Parasitic Ciliate Ichthyophthirius multifiliis Elicits Protective Immunity in Channel Catfish (Ictalurus punctatus). Clinical and Diagnostic Laboratory Immunology, 9: 176-181.
- Xu, D.H., & Klesius P.H. (2002). Antibody mediated immune response against Ichthyophthirius multifiliis using excised skin from channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius. Journal of Fish Diseases, 25: 299–306.
- Xu, D.H., & Klesius P.H. (2003). Protective effect of cutaneous antibody produced by channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius multifiliis Fouquet on cohabited non-immune catfish. Journal of Fish Diseases, 26: 287-291.
- Xu, D.H., Klesius, P.H., & Shelby, R.A. (2004). Immune responses and host protection of channelcatfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis after immunization with live theronts and sonicated trophonts. Diseases of Aquatic Organisms, 27: 135-141.
- Xu, D.H., Klesius, P.H., & Shoemaker, C.A. (2005). Cutaneous antibodies from channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius multifiliis (Ich) may induce apoptosis of Ich theronts. Journal of Fish Diseases, 28: 213-220.
- Xu, D.H., Klesius P.H., & Panangala, V.S. (2006). Induced cross-protection in channel catfish, Ictalurus punctatus (Rafinesque), against different immobilization serotypes of Ichthyophthirius multifiliis. Journal of Fish Diseases, 29: 131-138.
- Xu, D.H., Klesius, P.H. & Shoemaker, C.A. (2008). Protective immunity of Nile tilapia against Ichthyophthirius multifiliis post-immunization with live theronts and sonicated trophonts. Fish and Shellfish Immunology, 25: 124-127.
Ayrıntılar
| Bölüm | Makaleler |
|---|---|
| Yazarlar | |
| Yayımlanma Tarihi | 12 Haziran 2017 |
| Gönderilme Tarihi | 26 Ocak 2017 |
| Yayımlandığı Sayı | Yıl 2017Cilt: 34 Sayı: 2 |