Investigation of Spirogyra daedaleoides Czurda in terms of bioactive components

Tuğba Demiriz Yücer

doi:10.12714/egejfas.41.2.07

Research Article

Investigation of Spirogyra daedaleoides Czurda in terms of bioactive components

Year 2024, Volume: 41 Issue: 2, 142 - 147, 14.06.2024

Tuğba Demiriz Yücer

https://doi.org/10.12714/egejfas.41.2.07

Abstract

Algae stand out as suitable sources for use in the cosmetic, food and pharmaceutical industries due to their high content of components such as protein, polysaccharide, lipid, vitamin, mineral, amino acid, fatty acid, and carotenoid and due to the bioactive components that they produce. In this study, the bioactive properties of Spirogyra daedaleoides Czurda, located in the Charopyhta phylum of the algae, were examined. This species was isolated from the benthic habitats of the Yeşilırmak River and cultured. Antioxidant properties of Spirogyra daedaleoides was determined with the methods of free radical removal activity (DPPH, 2,2-diphenyl-1-picrylhydrazyl), iron (III) ion reduction power activity (FRAP, fluorescence recovery after photobleaching) and cation radical removal activity (ABTS, 2,2´-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)). The fatty acids of Spirogyra daedaleoides was determined by gas chromatography analysis, and vitamins were determined by high-performance liquid chromatography (HPLC) analysis. According to the results of antioxidant analysis, the free radical removal activity of Spirogyra daedaleoides, iron ion reduction power activity and cation radical removal activity were found to be high. When the fatty acid percentages of Spirogyra daedaleoides were considered, the ratios of C20:0 Arachidic acid, C16:0 Palmitic acid, C18:3n3 Alpha linoleic acid, C10:0 Caprinic acid and C18:2n6c Linoleic acid were found to be high. Spirogyra daedaleoides was found to be high in Vitamin A and Vitamin E. Spirogyra daedaleoides could be used as natural resources in the cosmetics, food and pharmaceutical industries according to the results.

Keywords

Charophyta, bioactive component, antioxidant, fatty acid, vitamin

Ethical Statement

Ethical approval is not required for this study.

Supporting Institution

The Scientific Research Projects (BAP) Unit of Karabuk University

Project Number

KBÜBAP-17-BM-159

Thanks

The author thanks Professor doctor Köksal Pabuçcu for his help in the diagnosis of algae species and Professor doctor Yavuz Beyatlı for laboratory equipment support.

References

Aaronson, S., Dhawale, S.W., Patni, N.J., Deangelis, B., Frank, O., & Baker, H. (1977). The cell content and secretion of water-soluble vitamins by several freshwater algae. Archives of microbiology, 112, 57-59. https://doi.org/10.1007/BF00446654
Andersen, R.A. (2005). Algal Culturing Techniques. Elsevier Academic Press.
Baytaşoğlu, H., & Başusta, N. (2015). The usage of marine organisms in medicine and pharmaceutics fields. Yunus Araştırma Bülteni B(2),71-80. https://doi.org/10.17693/yunusae.v15i21956.235767 (in Turkish with English abstract)
Brand-Williams, W., Cuvelier, M.E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und Technologie/Food Science and Technology, 28, 25 30. https://doi.org/10.1016/S0023-6438(95)80008-5
Bulut, Y. (2009). The investigations on the possibility of increase lipid content of Chlorella (Chlorophyceae). Master's Thesis, Çukurova University Institute of Science and Technology, Türkiye. (in Turkish with English abstract )
Canter-Lund H., & Lund J.W.G. (1995). Freshwater Algae. Their Microscopic World Explored. Biopress, Bristol. 360 p.
Demirel, G., & Özpınar, H. (2003). Algae and their use in animal nutrition. Uludağ University Journal of Faculty of Veterinary Medicine, 22(1-2-3), 103-108. (in Turkish with English abstract)
Demorais, M.G., Silvavaz, B.D., Morais, E.G., & Vieira Costa, J.A. (2015). Biologically active metabolites synthesized by microalgae, laboratory of microbiology and biochemistry. BioMed Research International, 75(3), 311–335. https://doi.org/10.1155/2015/835761
Erkaya, I.A., & Yalcin, D. (2021). Determination of nutritional evaluation fatty acid composition and growth kinetics of Spirogyra strains. Fresenius Environmental Bulletin, 8021.
Guiry, M.D. & Guiry, G.M. (2024). ‘AlgaeBase’, World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org
Gupta, S., Gupta, C., Garg, A.P., & Prakash, D. (2017). Probiotic efficiency of blue green algae on probiotics microorganisms. Journal of Microbiology and Experimentation, 4(4), 00120. https://doi.org/10.15406/jmen.2017.04.00120
Gülçin, İ. (2012). Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86(3), 345-391. https://doi.org/10.1007/s00204-011-0774-2
IUPAC (1979). Standarts methods for analysis of oils, fats and derivatives (6th ed.). New York: Pergamon Press.
Kapoor, R., & Mehta, U. (1993). Effect of supplementation of blue green alga (Spirulina) on outcome of pregnancy in rats. Plant Foods and Human Nutrition, 43(1), 29-35. https://doi.org/10.1007/BF01088093
Kumar J., Dhar P., Tayade A.B., Gupta D., Chaurasia O.P., & Upreti D.K. (2015). Chemical composition and biological activities of Trans-Himalayan alga Spirogyra porticalis (Muell.) Cleve. PLoS ONE, 10(2), e0118255. https://doi.org/10.1371/journal.pone.0118255
Lobban, C.S., Chapman, D.J., & Kremer, B.P. (1988). Experimental Phycology A Laboratory Manual. Chambridge University Press, London.
John, D.M., Whitton, B.A., & Brook, A.J. (2002). The freshwater algal flora of the British Isles, Cambridge University Press, Cambridge, 702 p.
Moreno, P., & Salvadó, V. (2000). Determination of eight water- and fat-soluble vitamins in multi-vitamin pharmaceutical formulations by high-performance liquid chromatography. Journal of Chromatography A, 870(1–2), 207–215. https://doi.org/10.1016/s0021-9673(99)01021-3
Oyaizu, M. (1986). Studies on products of browing reaction-antioxidative activities of products browing reaction prepared from glucosamine. Japanese Journal of Nutrition, 44, 307 315. https://doi.org/10.5264/eiyogakuzashi.44.307
Prescott, G.W. (1979). Freshwater Algae. Brown Comp. Pub., 293, Dubugue, Lawa.
Ramaraj, R., Tsai, D.D.W., & Chen, P.H. (2014). An exploration of the relationships between microalgae biomass growth and related environmental variables. Journal of Photochemistry and Photobiology B., 135, 44-47. https://doi.org/10.1016/j.jphotobiol.2014.04.001
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237. https://doi.org/10.1016/s0891-5849(98)00315-3
Rice-Evans, C.A., Miller, N.J., & Paganga, G. (1996). Structure antioxidant activity relationship of flavonoids and phenolic acids. Free Radical Biology and Medicine, 20(7), 933-956. https://doi.org/10.1016/0891-5849(95)02227-9
Thumvijit T., Inboot W., Peerapornpisal Y., Amornlerdpison D., & Wongpoomchai, R. (2013). The antimutagenic and antioxidant properties of Spirogyra neglecta (Hassall) Kützing. Journal of Medicinal Plants Research, 7, 2494 2500. https://doi.org/10.5897/JMPR2013.4427
Turan, G., & Cirik, S. (2018). Determination of vitamin composition of cultured macroalgae for Thalassotherapy applications. Ege Journal of Fisheries and Aquatic Sciences, 35 (2), 151 156. https://doi.org/10.12714/egejfas.2018.35.2.07 (in Turkish with English abstract)
Ünver Alçay, A., Bostan, K., Dinçel, E., & Varlık, C. (2017). Algae as a food source for humans. Aydın Gastronomy, 1(1), 47-59. (in Turkish with English abstract)

Spirogyra daedaleoides Czurda'nın biyoaktif bileşenler açısından incelenmesi