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Cytogenetic characteristics of endemic Squalius cappadocicus Özuluğ and Freyhof, 2011 in Türkiye

Year 2023, Volume: 40 Issue: 1, 51 - 55, 15.03.2023
https://doi.org/10.12714/egejfas.40.1.07

Abstract



In this study, a detailed chromosome analysis of the endemic Cappadocian Chub, Squalius cappadocicus in Melendiz Stream (Aksaray) was performed. The standard Giemsa staining, C-banding (CBG and CB-DAPI), and Ag-NOR technique were applied. The diploid chromosome number was 50; its karyotype formula was 14M + 16Sm + 10St + 10A. Heteromorphic sex chromosomes weren’t detected in the karyotype of the studied specimens. The number of all chromosomal arms (NF) was 90. In the standard C-banded and CB-DAPI karyotype of the species, dark C-bands were observed in the centromeric region of some bi-armed and acrocentric chromosomes, while slightly centromeric or pericentromeric C-bands were detected in some chromosomes. Three different active Ag-NORs, which were hemizygous, were detected in all samples examined. Two of these active NORs were detected in the bi-armed and the other in the acrocentric chromosome short arm. The Ag-NOR number of this species was evaluated as a feature that distinguishes it from other Squalius species in Türkiye.


Supporting Institution

Coordination Committee Scientific Research Projects of Selcuk University

Thanks

This study was financed with a grants from the Scientific Research Projects Coordination Board of Selçuk University (Project number: 21211009)

References

  • Arslan, A., & Arslan, E. (2007). Importance of C-banding (Constitutive Heterochromatin) in karyosystematic (in Turkish with English abstract). Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 2(29), 121-126.
  • Bertollo, L., Cioffi, M., & Moreira-Filho, O. (2015). Direct chromosome preparation from freshwater teleost fishes. In C. Ozouf-Costaz, E. Pisano, F. Foresti, & L. Almeida Toledo (Eds.), Fish Cytogenetic Techniques (Chondrichthyans and Teleosts) (pp. 21-26). United States: CRC Press.
  • Bianco, P. G., Aprea, G., Balletto, E., Capriglione, T., Fulgione, D., & Odierna, G. (2004). The karyology of the cyprinid genera Scardinius and Rutilus in Southern Europe. Ichthyological Research, 51, 274-278. https://doi.org/10.1007/s10228-004-0221-y
  • Boroń, A., Porycka, K., Ito, D., Abe, S., & Kirtiklis, L. (2009). Comparative molecular cytogenetic analysis of three Leuciscus species (Pisces, Cyprinidae) using chromosome banding and FISH with rDNA. Genetica, 135, 199-207. https://doi.org/10.1007/s10709-008-9269-3
  • Cataudella, S., Sola, L., Muratori, R.A., & Capanna, E. (1977). The chromosomes of 11 species of Cyprinidae and one Cobitidae from Italy, with some remarks on the problem of polyploidy in the Cypriniformes. Genetica, 47(3), 161-171. https://doi.org/10.1007/BF00123236
  • Çiçek, E., Fricke, R., Sungur, S., & Eagderı, S. (2018). Endemic freshwater fishes of Turkey. FishTaxa, 3(4), 1-39.
  • Collares-Pereira, M.J., Próspero, M., Biléu, R., & Rodrigues, E. (1998). Leuciscus (Pisces, Cyprinidae) karyotypes: transect of Portuguese populations. Genetics and Molecular Biology, 21(1), 63-69. https://doi.org/10.1590/S1415-47571998000100011
  • Doori, A.S.J., & Arslan, A. (2022). Karyotypes and ZZ/ZW sex chromosome system of endemic Squalius recurvirostris (Leuciscinae, Cyprinidae) in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(4), 649-657. https://doi.org/10.18016/ksutarimdoga.vi.915278
  • Howell, W.M., & Black, D. A. (1980). Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36, 1014-1015. https://doi.org/10.1007/BF01953855
  • Karasu Ayata, M. (2020). Comparative cytogenetics of two Squalius Bonaparte, 1837 species (Cypriniformes: Leuciscidae). Iranian Journal of Science and Technology, Transactions A: Science, 44, 355-360. https://doi.org/10.1007/s40995-020-00836-0
  • Kılıç, D., & Şişman, T. (2016). Karyotype analysis of chub, Squalius cephalus (Linnaeus, 1758)(Teleostei: Cyprinidae) from Karasu River, Erzurum, Turkey. Caspian Journal of Environmental Sciences, 14(2), 95-103.
  • Kılıç Demirok, N. (2000). The Studies on the chromosomes of the some cyprinid species and subspecion of the tigris river systems. Doctoral dissertation, Dicle University, Turkey. (in Turkish wish English abstract)
  • Levan, A., Fredga, K., & Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52(2), 201-220. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
  • Nabais, C., Rampin, M., & Collares-Pereira, M.J. (2013). Comparative cytogenetics of two endangered leuciscine fish, Squalius aradensis and S. torgalensis (Teleostei, Cyprinidae), from the Iberian Peninsula. Comparative Cytogenetics, 7(1), 33-42. https://doi.org/10.3897/CompCytogen.v7i1.4672
  • Özuluğ, M., & Freyhof, J. (2011). Review of the genus Squalius in Western and Central Anatolia, with description of four new species (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters, 22(2), 107-148.
  • Pekol, S. & Arslan, O. (2014). Squalius cephalus (L., 1758)’un nor fenotipi ve sucul ortam ekotoksikolojik çalışmalar açısından değerlendirilmesi (Kastamonu Beyler Barajı Populasyonu). Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 2, 23-28.
  • Ráb, P., Rábová, M., Pereira, C.S., Collares-Pereira, M.J., & Pelikánová, Š. (2008). Chromosome studies of European cyprinid fishes: interspecific homology of leuciscine cytotaxonomic marker—the largest subtelocentric chromosome pair as revealed by cross-species painting. Chromosome Research, 16, 863-873. https://doi.org/10.1007/s10577-008-1245-3
  • Rossi, A.R., Milana, V., Hett, A. K., & Tancioni, L. (2012). Molecular cytogenetic analysis of the Appenine endemic cyprinid fish Squalius lucumonis and three other Italian leuciscines using chromosome banding and FISH with rDNA probes. Genetica, 140(10), 469-476. https://doi.org/10.1007/s10709-012-9695-0
  • Sofradzija, A. (1977). Kariologia i citotaksonomija vrsta roda Leuciscus iź voda Bosne i Hercegovine. Godišnjak Biološkog Instituta Univerziteta u Sarajevu Posebno izdanje, 30, 113-211.
  • Sumner, A. (1972). A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304-306. https://doi.org/10.1016/0014-4827(72)90558-7
  • Ünal, S., & Gaffaroğlu, M. (2016). Karyology of six cyprinid fishes from Seyhan and Ceyhan rivers in Anatolia. Caryologia, 69(4), 362-369. https://doi.org/10.1080/00087114.2016.1247328
  • Ünal Karakuş, S. & Gaffaroğlu, M. (2021). Karyotype, C-band and NOR phenotype of Anatolian endemic fish Squalius anatolicus (Bogutskaya, 1997) (Teleostei, Leuciscidae). Ege Journal of Fisheries and Aquatic Sciences, 38(3), 311-315. https://doi.org/10.12714/egejfas.38.3.07
  • Vujosevic, M., Zivkovic, S., Rimsa, D., Jurisic, S., & Cakic, P. (1983). The chromosomes of 9 fish species from Dunav basin in Yugoslavia. Ichthyologia, 15(2), 29-40.
  • Wolf, U., Ritter, H., Atkin, N., & Ohno, S. (1969). Polyploidization in the fish family Cyprinidae, order Cypriniformes. Humangenetik, 7, 240-244. . https://doi.org/10.1007/BF00273173

Türkiye'ye endemik Squalius cappadocicus Özuluğ and Freyhof, 2011'un sitogenetik özellikleri

Year 2023, Volume: 40 Issue: 1, 51 - 55, 15.03.2023
https://doi.org/10.12714/egejfas.40.1.07

Abstract



Bu çalışmada, Melendiz Çayı'nda (Aksaray) endemik Kapadokya Kefali, Squalius cappadocicus'un detaylı kromozom analizi yapıldı.  Standart Giemsa boyama, C-bantlama (CBG ve CB-DAPI) ve Ag-NOR tekniği uygulandı. Diploid kromozom sayısı 50’dir; karyotip formülü 14M + 16Sm + 10St + 10A’dir. İncelenen örneklerin karyotipinde heteromorfik cinsiyet kromozomları saptanmadı. Tüm kromozom kollarının (NF) sayısı 90'dı. Türün standart C-bantlı ve CB-DAPI karyotipinde, bazı çift kollu ve akrosentrik kromozomların sentromer bölgesinde koyu renkli C-bantları gözlenirken, bazı kromozomlarda sentromerik veya perisentromerik hafif C-bantlar tespit edildi. İncelenen tüm örneklerde hemizigot olan üç farklı aktif Ag-NOR tespit edildi. Bu aktif NOR'lardan ikisi iki kollu, diğeri akrosentrik kromozom kısa kolunda tespit edildi. Bu türün Ag-NOR sayısı, onu Türkiye'deki diğer Squalius türlerinden ayıran bir özellik olarak değerlendirilmiştir.


References

  • Arslan, A., & Arslan, E. (2007). Importance of C-banding (Constitutive Heterochromatin) in karyosystematic (in Turkish with English abstract). Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 2(29), 121-126.
  • Bertollo, L., Cioffi, M., & Moreira-Filho, O. (2015). Direct chromosome preparation from freshwater teleost fishes. In C. Ozouf-Costaz, E. Pisano, F. Foresti, & L. Almeida Toledo (Eds.), Fish Cytogenetic Techniques (Chondrichthyans and Teleosts) (pp. 21-26). United States: CRC Press.
  • Bianco, P. G., Aprea, G., Balletto, E., Capriglione, T., Fulgione, D., & Odierna, G. (2004). The karyology of the cyprinid genera Scardinius and Rutilus in Southern Europe. Ichthyological Research, 51, 274-278. https://doi.org/10.1007/s10228-004-0221-y
  • Boroń, A., Porycka, K., Ito, D., Abe, S., & Kirtiklis, L. (2009). Comparative molecular cytogenetic analysis of three Leuciscus species (Pisces, Cyprinidae) using chromosome banding and FISH with rDNA. Genetica, 135, 199-207. https://doi.org/10.1007/s10709-008-9269-3
  • Cataudella, S., Sola, L., Muratori, R.A., & Capanna, E. (1977). The chromosomes of 11 species of Cyprinidae and one Cobitidae from Italy, with some remarks on the problem of polyploidy in the Cypriniformes. Genetica, 47(3), 161-171. https://doi.org/10.1007/BF00123236
  • Çiçek, E., Fricke, R., Sungur, S., & Eagderı, S. (2018). Endemic freshwater fishes of Turkey. FishTaxa, 3(4), 1-39.
  • Collares-Pereira, M.J., Próspero, M., Biléu, R., & Rodrigues, E. (1998). Leuciscus (Pisces, Cyprinidae) karyotypes: transect of Portuguese populations. Genetics and Molecular Biology, 21(1), 63-69. https://doi.org/10.1590/S1415-47571998000100011
  • Doori, A.S.J., & Arslan, A. (2022). Karyotypes and ZZ/ZW sex chromosome system of endemic Squalius recurvirostris (Leuciscinae, Cyprinidae) in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(4), 649-657. https://doi.org/10.18016/ksutarimdoga.vi.915278
  • Howell, W.M., & Black, D. A. (1980). Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36, 1014-1015. https://doi.org/10.1007/BF01953855
  • Karasu Ayata, M. (2020). Comparative cytogenetics of two Squalius Bonaparte, 1837 species (Cypriniformes: Leuciscidae). Iranian Journal of Science and Technology, Transactions A: Science, 44, 355-360. https://doi.org/10.1007/s40995-020-00836-0
  • Kılıç, D., & Şişman, T. (2016). Karyotype analysis of chub, Squalius cephalus (Linnaeus, 1758)(Teleostei: Cyprinidae) from Karasu River, Erzurum, Turkey. Caspian Journal of Environmental Sciences, 14(2), 95-103.
  • Kılıç Demirok, N. (2000). The Studies on the chromosomes of the some cyprinid species and subspecion of the tigris river systems. Doctoral dissertation, Dicle University, Turkey. (in Turkish wish English abstract)
  • Levan, A., Fredga, K., & Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52(2), 201-220. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
  • Nabais, C., Rampin, M., & Collares-Pereira, M.J. (2013). Comparative cytogenetics of two endangered leuciscine fish, Squalius aradensis and S. torgalensis (Teleostei, Cyprinidae), from the Iberian Peninsula. Comparative Cytogenetics, 7(1), 33-42. https://doi.org/10.3897/CompCytogen.v7i1.4672
  • Özuluğ, M., & Freyhof, J. (2011). Review of the genus Squalius in Western and Central Anatolia, with description of four new species (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters, 22(2), 107-148.
  • Pekol, S. & Arslan, O. (2014). Squalius cephalus (L., 1758)’un nor fenotipi ve sucul ortam ekotoksikolojik çalışmalar açısından değerlendirilmesi (Kastamonu Beyler Barajı Populasyonu). Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 2, 23-28.
  • Ráb, P., Rábová, M., Pereira, C.S., Collares-Pereira, M.J., & Pelikánová, Š. (2008). Chromosome studies of European cyprinid fishes: interspecific homology of leuciscine cytotaxonomic marker—the largest subtelocentric chromosome pair as revealed by cross-species painting. Chromosome Research, 16, 863-873. https://doi.org/10.1007/s10577-008-1245-3
  • Rossi, A.R., Milana, V., Hett, A. K., & Tancioni, L. (2012). Molecular cytogenetic analysis of the Appenine endemic cyprinid fish Squalius lucumonis and three other Italian leuciscines using chromosome banding and FISH with rDNA probes. Genetica, 140(10), 469-476. https://doi.org/10.1007/s10709-012-9695-0
  • Sofradzija, A. (1977). Kariologia i citotaksonomija vrsta roda Leuciscus iź voda Bosne i Hercegovine. Godišnjak Biološkog Instituta Univerziteta u Sarajevu Posebno izdanje, 30, 113-211.
  • Sumner, A. (1972). A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304-306. https://doi.org/10.1016/0014-4827(72)90558-7
  • Ünal, S., & Gaffaroğlu, M. (2016). Karyology of six cyprinid fishes from Seyhan and Ceyhan rivers in Anatolia. Caryologia, 69(4), 362-369. https://doi.org/10.1080/00087114.2016.1247328
  • Ünal Karakuş, S. & Gaffaroğlu, M. (2021). Karyotype, C-band and NOR phenotype of Anatolian endemic fish Squalius anatolicus (Bogutskaya, 1997) (Teleostei, Leuciscidae). Ege Journal of Fisheries and Aquatic Sciences, 38(3), 311-315. https://doi.org/10.12714/egejfas.38.3.07
  • Vujosevic, M., Zivkovic, S., Rimsa, D., Jurisic, S., & Cakic, P. (1983). The chromosomes of 9 fish species from Dunav basin in Yugoslavia. Ichthyologia, 15(2), 29-40.
  • Wolf, U., Ritter, H., Atkin, N., & Ohno, S. (1969). Polyploidization in the fish family Cyprinidae, order Cypriniformes. Humangenetik, 7, 240-244. . https://doi.org/10.1007/BF00273173

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Ahmed Sadeq Jaber DOORİ
SELCUK UNIVERSITY
0000-0002-7452-657X
Türkiye


Atilla ARSLAN
DEPARTMENT OF BIOLOGY
0000-0002-4766-4969
Türkiye

Project Number 21211009
Publication Date March 15, 2023
Submission Date January 5, 2023
Published in Issue Year 2023Volume: 40 Issue: 1

Cite

APA
DOORİ, A. S. J., & ARSLAN, A. (2023). Cytogenetic characteristics of endemic Squalius cappadocicus Özuluğ and Freyhof, 2011 in Türkiye. Ege Journal of Fisheries and Aquatic Sciences, 40(1), 51-55. https://doi.org/10.12714/egejfas.40.1.07