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Determination of quorum sensing system via biosensor strains and virulence factors in fish pathogen Aeromonas hydrophila

Year 2020, Volume 37, Issue 1, 29 - 36, 15.03.2020
https://doi.org/10.12714/egejfas.37.1.04

Abstract



Quorum Sensing is a system that produces critical virulence factors, virulent get bacteria and manages the disease as a result, and when they realize that the bacteria reach the majority they want by enabling them to communicate with the signal molecules themselves. In this study, Quorum Sensing system of Aeromonas hydrophila (2 strains) which is the causative agent of fish infection Motile Aeromonas Septicemia (MAS) disease was studied. In the strains, primarily the production of N-butanoyl-L-homoserine lactone (BHL) and N-(3-octododecanoyl)-L-homoserine lactone (OdDHL) signaling molecules was investigated via Chromobacterium violaceum CV026 and Agrobacterium tumafeciens NT1 biosensor strains. A. hydrophila produced BHL signaling molecule in assay committed using C. violaceum CV026 strain, producing OdDHL signaling molecule in assay committed using A. tumefaciens NT1 strain. A. hydrophila was investigated as phenotypically by the detection of BHL and OdDHL signaling molecules and in the presence of virulence factors controlled by quorum sensing system such as ramnolipid, elastase, protease, amylase, hemolysis production dependent on these molecules. The ramnolipid, protease, amylase and hemolysis activities of A. hydrophila strains were found to be positive. A. hydrophila has less elastase activity than Pseudomonas aeruginosa PAO1 control strain. Research has emphasizing A. hydrophila strains are within a population and that they have a of quorum sensing system, shown that they act collectively that determined they produces dangerous virulence factors that cause disease in fish.




References

  • Abdelhamed, H.A., Banes, M., Karsi, A. & Lawrence, M.L. (2019). Recombinant ATPase of virulent Aeromonas hydrophila protects channel catfish against motile Aeromonas septicemia. Frontiers in Immunology. DOI: 10.3389/fimmu.2019.01641
  • Albert, M.J., Ansaruzzaman, M., Talukder, K.A., Chopra, A.K., Kuhn, I., Rahman, M., Faruque, A.S.G. Islam, M.S., Sack, R.B. & Mollby, R. (2000). Prevalence of Enterotoxin Genes in Aeromonas spp. Isolated from Children with Diarrhea, Healthy Controls, and the Environment. Journal of Clinical Microbiology, 38(10), 3785-3790.
  • Al-Fatlawy, H.N.K., & Al-Hadrawy, H.A. (2014). Isolation and characterization of A. hydrophila from the Al-Jadryia river in Baghdad (Iraq). American Journal of Educational Research, 2(8), 658-662. DOI: 10.12691/education-2-8-14
  • Allan, B.J. & Stevenson, R.M.W. (1981). Extracellular virulence factors of Aeromonas hydrophila in fish infections. Canadian Journal of Microbiology, 27(10), 1114-1122.
  • Altun, H.U. & Şener, B. (2008). Biyofilm infeksiyonları ve antibiyotik direnci. Hacettepe Tıp Dergisi, 39, 82-88.
  • Baldissera, M.D., Souza, C.F., Abbad, L.B., Verdi, C.M., Santos, R.C.V, Da Silva, A.S. & Baldisserotto, B. (2019). Dietary supplementation with caffeine increases survival rate, reduces microbial load and protects the liver against Aeromonas hydrophila-induced hepatic damage in the grass carp Ctenopharyngodon idella. Microbial Pathogenesis, 135, 103637.
  • Bastardo, A., Ravelo, C., Castro, N., Calheiros, J. & Romalde, J.L. (2012). Effectiveness of bivalent vaccines against Aeromonas hydrophila and Lactococcus garvieae infections in rainbow trout Oncorhynchus mykiss (Walbaum). Fish & Shellfish Immunology, 32(5), 756-761. DOI: 10.1016/j.fsi.2012.01.028
  • Boşgelmez-Tınaz, G. (2003). Quorum Sensing in Gram-Negative Bacteria. Turkish of Journal Biology. 27, 85-93.
  • Bruhn, J.B, Dalsgaard, I., Nielsen, K.F., Buchholtz, C., Larsen J.L. & Gram L. (2005). Quorum sensing signal molecules (acylated homoserine lactones) in Gram negative fish pathogenic bacteria. Diseases of Aquatic Organisms, 65, 43-52. DOI: 10.3354/dao065043
  • Cha, C., Gao, P., Chen, Y.C., Shaw, P.D. & Farrand, S.K. (1998). Production of acylhomoserine lactone signals by gram-negative plant-associated bacteria. Molecular Plant-Microbe Interactions, 11, 1119-1129.
  • Chen, B., Peng, M., Tong, W., Zhang, Q. & Song, Z. (2019). The Quorum Quenching Bacterium Bacillus licheniformis T-1 Protects Zebrafish against Aeromonas hydrophila Infection. Probiotics and Antimicrobial Proteins. DOI: 10.1007/s12602-018-9495-7
  • Chopra, A.K., Xu, X.J., Ribardo, D., Gonzalez, M, Kuhl, K., Peterson, J. W. & Houston, C. W. (2000). The Cytotoxic Enterotoxin of Aeromonas hydrophila Induces Proinflammatory Cytokine Production and Activates Arachidonic Acid Metabolism in Macrophages. Infection and Immunity, 68(5), 2808-2818.
  • Chu, W., Jiang, Y., Yongwang, L. & Zhu, W. (2011). Role of the quorum-sensing system in biofilm formation and virulence of Aeromonas hydrophila. African Journal of Microbiology Research, 5(32), 5819-5825, DOI: 10.5897/AJMR10.684
  • Daskalov, H. (2006). The importance of Aeromonas hydrophila in food safety. Food Control, 17(6), 474–483. DOI: 10.1016/j.foodcont.2005.02.009
  • De Silva, B.C.J., Hossain, S., Dahanayake, P.S. & Heo, G.J. (2018). Aeromonas spp. from marketed Yesso scallop (Patinopecten yessoensis): molecular characterization, phylogenetic analysis, virulence properties andantimicrobial susceptibility. Journal of Applied Microbiology, 126, 288-299. DOI: 10.1111/jam.14106
  • Dewi, A.F., Prajitno, A. & Yuniarti, A.A. (2019). Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila. The Journal of Experimental Life Science, 9(2), ISSN:2087-2852.
  • Dong, Y., Zhang, X., Soo, H.L., Greenberg, P. & Zhang, L. (2005). The two-component response regulator PprB modulates quorum sensing signal production and global gene expression in P. aeruginosa. Molecular Microbiology, 56, 1287-1301. DOI: 10.1111/j.1365-2958.2005.04612.x
  • Erdem, B., Kariptaş, E. & Kaya, T. (2010). Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turkish Journal of Biology, 34, 453-462. DOI: 10.3906/biy-0901-20
  • Gong, Y.X., Zhu, B., Liu, G.L., Liu, L., Ling, F. & Wang, G.X. (2015). Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effects of a recombinant vaccine against Aeromonas hydrophila. Fish & Shellfish Immunology, 42(1), 213-220. DOI: 10.1016/j.fsi.2014.11.004
  • Hatha, M., Vivekanandhan, A.A., & Christol, G.J.J. (2005). Antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish. International Journal of Food Microbiology, 98(2), 131-134. DOI: 10.1016/j.ijfoodmicro.2004.05.017
  • Hazen, T. C., Flierman, C. B., Hirsch, R. P., & Esch, G. W. (1978). Prevalence and distribution of Aeromonas hydrophila in the United States. Applied and Environmental Microbiology, 36, 731-738.
  • Hellingwerf, K.J. (2005). Bacterial observations: a rudimentary from of intelligence?. Trends in Microbiology, 13(4), 152-158. DOI: 10.1016/j.tim.2005.02.001
  • İkinci, Ö. (2010). Bakteriler Koku Alıyor. Yaklaşan Saat, Bilim ve Teknik, Güncelleme: 06.09.2010,http://www.yaklasansaat.com/haberdosya/2010_haberleri/eylul_2010/eylul7.asp (20.03.2019).
  • Karatuna, O. & Yağcı, A. (2008). Pseudomonas aeruginosa’da virülans faktörleri ve quorum sensing (Virulence factors of Pseudomonas aeruginosa and quorum sensing). Türk Mikrobiyoloji Cemiyeti Dergisi, 38(1), 42-51.
  • Kozlova, E.V., Popov, V.L., Sha, J., Foltz, S.M., Erova, T.E., Agar, S.L, Horneman, A.J. & Chopra, A.K. (2008). Mutation in the S-ribosylhomocysteinase (luxS) gene involved in quorum sensing affects biofilm formation and virulence in a clinical isolate of Aeromonas hydrophila. Microbial Pathogenesis, 45(5–6), 343-354. DOI: 10.1016/j.micpath.2008.08.007
  • Mathewson, J.J. & Dupont, H.L. (1992). Aeromonas species: role as human pathogens, In: Remington, J.S., Swartz, M.N. (Ed.), Current Clinical Topics in Infectious Diseases, (pp 26-36), Vol12e. Cambridge: Blackwell Scientific.
  • McClean, K.H., Winson, M.K., Fish, L., Taylor, A., Chhabra, S.R., Camara, M., Daykin, M., Lamb, J.H., Swift, S., Bycroft, B.W., Stewart, G.S.A.B. & Williams, P. (1997). Quorum sensing and Chromobacterium violeceum: exploitation of violacein production for the detection of N-acylhomoserine lactones. Microbiology, 143, 3703-3711. DOI: 10.1099/00221287-143-12-3703
  • Moghaddam, M.M., Khodi, S. & Mirhosseini, A. (2014). Quorum Sensing in Bacteria and a Glance on Pseudomonas aeruginosa. Clinical Microbiology: Open Access, 3(4), ISSN: 2327-5073. DOI: 10.4172/2327-5073.1000156
  • Nijland, R. (2010). Bacteria can have a 'sense of smell'. Science Daily, Science News, 17 August 2010. <www.sciencedaily.com/releases/2010/08/100816095719.htm>.
  • Ohman, D.E., Cryz, S.J. & Iglewski, B.H. (1980). Isolation and characterization of a P. aeruginosa PAO mutant that produces altered elastase. Journal of Bacteriology, 142, 836- 842.
  • Pandey, A., Naik, M. & Dubey, S.K. (2010). Hemolysin, Protease, and EPS Producing Pathogenic Aeromonas hydrophila Strain An4 Shows Antibacterial Activity against Marine Bacterial Fish Pathogens. Journal of Marine Biology, Article ID 563205, 9 pages. DOI: 10.1155/2010/563205
  • Rahim, Z., Sanyal, S.C., Aziz, K.M., Huq, M.I. & Chowdhury, A.A. (1984). Isolation of enterotoxigenic, hemolytic, and antibiotic-resistant Aeromonas hydrophila strains from infected fish in Bangladesh. Applied and Environmental Microbiology, 48(4), 865-867.
  • Salyers, A.A. & Whitt, D.D. (1994). Bacterial pathogenesis: a molecular approach. ASM Press, Washington, D. C., USA, pp. 260-268.
  • Saraçlı, M.A. (2006). "Quorum sensing": mikroorganizmalar iletişim mi kuruyor? Gülhane Tıp Dergisi, 48(4), 244-250.
  • Schwenteit, J., Gram, L., Nielsen, K.F., Fridjonsson, O.H., Bornscheuer, U.T., Givskov, M. & Gudmundsdottir B.K. (2011). Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence. Veterinary Microbiology, 147, 389-397. DOI: 10.1016/j.vetmic.2010.07.020
  • Siegmund, I. & Wagner, F. (1991). New methods for detecting rhamnolipids excreted by Pseudomonas species during growth in mineral agar. BioTechniques, 5, 265-268.
  • Swift, S., Lynch, M.J., Fish, L., Kirke, D.F., Tomas, J.M., Stewart, G.S.A.B. & Williams, P. (1999). Quorum Sensing-Dependent Regulation and Blockade of Exoprotease Production in Aeromonas hydrophila. Infection and Immunity, 67(10), 5192-5199.
  • Swift, S., Karlyshev, A.V., Fish, L., Durant, E. L., Winson, M. K., Chhabra, S. R., Williams, P., Macintyre, S., & Stewart, G. S. A. B. (1997). Quorum Sensing in Aeromonas hydrophila and Aeromonas salmonicida: Identification of the LuxRI Homologs AhyRI and AsaRI and Their Cognate N-Acylhomoserine Lactone Signal Molecules. Journal of Bacteriology, 179(17), 5271-5281. DOI: 10.1128/jb.179.17.5271-5281.1997
  • Timur, G., & Timur, M. (2003). Balık Hastalıkları, İstanbul Üniversitesi Su Ürünleri Fakültesi, Yayın No: 5, s.538, İstanbul.
  • Ulusoy, S. (2007). Yoğun Bakım Ünitelerinden İzole Edilen Pseudomonas aeruginosa Suşlarında N-Açil Homoserin Lakton Üretiminin Araştırılması. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, 100s, Isparta.
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  • Zhang, X., Yang, W., Wu, H., Gong, X. & Li, A. (2014). Multilocus sequence typing revealed a clonal lineage of Aeromonas hydrophila, caused motile Aeromonas septicemia outbreaks in pond-cultured cyprinid fish in an epidemic area in central China. Aquaculture, 432, 1-6. DOI: 10.1016/j.aquaculture.2014.04.017

Aeromonas hydrophila balık patojeninde biyosensör suşlar aracılığıyla çevreyi algılama sisteminin tespiti ve virülens faktörleri

Year 2020, Volume 37, Issue 1, 29 - 36, 15.03.2020
https://doi.org/10.12714/egejfas.37.1.04

Abstract



Çevreyi algılama sistemi bakterilerin sinyal molekülleriyle kendi aralarında iletişim kurmalarını sağlayarak istedikleri çoğunluğa ulaştıklarını anladıkları anda da kritik virülens faktörlerini üreten, bakteriyi virülent hale getiren, sonucunda hastalık oluşturan ve bunu yöneten bir sistemdir. Araştırmada balık enfeksiyonu Hareketli Aeromonas Septisemisi (MAS) etkeni Aeromonas hydrophila (2 suş) suşlarının çevreyi algılama sistemi çalışılmıştır. Suşlarda öncelikle N-butanoyl-L-homoserin lakton (BHL) ve N-(3-okzododekanoyl)-L-homoserin lakton (OdDHL) sinyal moleküllerinin üretimi, Chromobacterium violaceum CV026 ve Agrobacterium tumafeciens NT1 biyosensör suşları aracılığıyla araştırılmıştır. A. hydrophila’nın C. violaceum CV026 suşu kullanılarak yapılan testte BHL sinyal molekülünü ürettiği, A. tumefaciens NT1 suşu kullanılarak yapılan testte OdDHL sinyal molekülünü ürettiği tespit edilmiştir. A. hydrophila’da BHL ve OdDHL sinyal moleküllerinin tespitiyle bu moleküllere bağımlı olan ramnolipid, elastaz, proteaz, amilaz, hemoliz üretimi gibi çevreyi algılama sistemi tarafından kontrol edilen virülens faktörlerinin varlığı bakımından fenotipik olarak araştırılmıştır. A. hydrophila suşlarının ramnolipid, proteaz, amilaz ve hemoliz aktiviteleri pozitif olarak bulunmuştur. A. hydrophila, Pseudomonas aeruginosa PAO1 kontrol suşuna göre daha az elastaz aktivitesine sahiptir. Araştırmayla A. hydrophila suşlarının bir topluluk içerisinde olduğunu anladığının ve toplu olarak hareket ettiğinin göstergesi olan çevreyi algılama sistemine sahip olduğu, balıklarda hastalık oluşturan tehlikeli virülens faktörlerini ürettiği belirlenmiştir.




References

  • Abdelhamed, H.A., Banes, M., Karsi, A. & Lawrence, M.L. (2019). Recombinant ATPase of virulent Aeromonas hydrophila protects channel catfish against motile Aeromonas septicemia. Frontiers in Immunology. DOI: 10.3389/fimmu.2019.01641
  • Albert, M.J., Ansaruzzaman, M., Talukder, K.A., Chopra, A.K., Kuhn, I., Rahman, M., Faruque, A.S.G. Islam, M.S., Sack, R.B. & Mollby, R. (2000). Prevalence of Enterotoxin Genes in Aeromonas spp. Isolated from Children with Diarrhea, Healthy Controls, and the Environment. Journal of Clinical Microbiology, 38(10), 3785-3790.
  • Al-Fatlawy, H.N.K., & Al-Hadrawy, H.A. (2014). Isolation and characterization of A. hydrophila from the Al-Jadryia river in Baghdad (Iraq). American Journal of Educational Research, 2(8), 658-662. DOI: 10.12691/education-2-8-14
  • Allan, B.J. & Stevenson, R.M.W. (1981). Extracellular virulence factors of Aeromonas hydrophila in fish infections. Canadian Journal of Microbiology, 27(10), 1114-1122.
  • Altun, H.U. & Şener, B. (2008). Biyofilm infeksiyonları ve antibiyotik direnci. Hacettepe Tıp Dergisi, 39, 82-88.
  • Baldissera, M.D., Souza, C.F., Abbad, L.B., Verdi, C.M., Santos, R.C.V, Da Silva, A.S. & Baldisserotto, B. (2019). Dietary supplementation with caffeine increases survival rate, reduces microbial load and protects the liver against Aeromonas hydrophila-induced hepatic damage in the grass carp Ctenopharyngodon idella. Microbial Pathogenesis, 135, 103637.
  • Bastardo, A., Ravelo, C., Castro, N., Calheiros, J. & Romalde, J.L. (2012). Effectiveness of bivalent vaccines against Aeromonas hydrophila and Lactococcus garvieae infections in rainbow trout Oncorhynchus mykiss (Walbaum). Fish & Shellfish Immunology, 32(5), 756-761. DOI: 10.1016/j.fsi.2012.01.028
  • Boşgelmez-Tınaz, G. (2003). Quorum Sensing in Gram-Negative Bacteria. Turkish of Journal Biology. 27, 85-93.
  • Bruhn, J.B, Dalsgaard, I., Nielsen, K.F., Buchholtz, C., Larsen J.L. & Gram L. (2005). Quorum sensing signal molecules (acylated homoserine lactones) in Gram negative fish pathogenic bacteria. Diseases of Aquatic Organisms, 65, 43-52. DOI: 10.3354/dao065043
  • Cha, C., Gao, P., Chen, Y.C., Shaw, P.D. & Farrand, S.K. (1998). Production of acylhomoserine lactone signals by gram-negative plant-associated bacteria. Molecular Plant-Microbe Interactions, 11, 1119-1129.
  • Chen, B., Peng, M., Tong, W., Zhang, Q. & Song, Z. (2019). The Quorum Quenching Bacterium Bacillus licheniformis T-1 Protects Zebrafish against Aeromonas hydrophila Infection. Probiotics and Antimicrobial Proteins. DOI: 10.1007/s12602-018-9495-7
  • Chopra, A.K., Xu, X.J., Ribardo, D., Gonzalez, M, Kuhl, K., Peterson, J. W. & Houston, C. W. (2000). The Cytotoxic Enterotoxin of Aeromonas hydrophila Induces Proinflammatory Cytokine Production and Activates Arachidonic Acid Metabolism in Macrophages. Infection and Immunity, 68(5), 2808-2818.
  • Chu, W., Jiang, Y., Yongwang, L. & Zhu, W. (2011). Role of the quorum-sensing system in biofilm formation and virulence of Aeromonas hydrophila. African Journal of Microbiology Research, 5(32), 5819-5825, DOI: 10.5897/AJMR10.684
  • Daskalov, H. (2006). The importance of Aeromonas hydrophila in food safety. Food Control, 17(6), 474–483. DOI: 10.1016/j.foodcont.2005.02.009
  • De Silva, B.C.J., Hossain, S., Dahanayake, P.S. & Heo, G.J. (2018). Aeromonas spp. from marketed Yesso scallop (Patinopecten yessoensis): molecular characterization, phylogenetic analysis, virulence properties andantimicrobial susceptibility. Journal of Applied Microbiology, 126, 288-299. DOI: 10.1111/jam.14106
  • Dewi, A.F., Prajitno, A. & Yuniarti, A.A. (2019). Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila. The Journal of Experimental Life Science, 9(2), ISSN:2087-2852.
  • Dong, Y., Zhang, X., Soo, H.L., Greenberg, P. & Zhang, L. (2005). The two-component response regulator PprB modulates quorum sensing signal production and global gene expression in P. aeruginosa. Molecular Microbiology, 56, 1287-1301. DOI: 10.1111/j.1365-2958.2005.04612.x
  • Erdem, B., Kariptaş, E. & Kaya, T. (2010). Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turkish Journal of Biology, 34, 453-462. DOI: 10.3906/biy-0901-20
  • Gong, Y.X., Zhu, B., Liu, G.L., Liu, L., Ling, F. & Wang, G.X. (2015). Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effects of a recombinant vaccine against Aeromonas hydrophila. Fish & Shellfish Immunology, 42(1), 213-220. DOI: 10.1016/j.fsi.2014.11.004
  • Hatha, M., Vivekanandhan, A.A., & Christol, G.J.J. (2005). Antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish. International Journal of Food Microbiology, 98(2), 131-134. DOI: 10.1016/j.ijfoodmicro.2004.05.017
  • Hazen, T. C., Flierman, C. B., Hirsch, R. P., & Esch, G. W. (1978). Prevalence and distribution of Aeromonas hydrophila in the United States. Applied and Environmental Microbiology, 36, 731-738.
  • Hellingwerf, K.J. (2005). Bacterial observations: a rudimentary from of intelligence?. Trends in Microbiology, 13(4), 152-158. DOI: 10.1016/j.tim.2005.02.001
  • İkinci, Ö. (2010). Bakteriler Koku Alıyor. Yaklaşan Saat, Bilim ve Teknik, Güncelleme: 06.09.2010,http://www.yaklasansaat.com/haberdosya/2010_haberleri/eylul_2010/eylul7.asp (20.03.2019).
  • Karatuna, O. & Yağcı, A. (2008). Pseudomonas aeruginosa’da virülans faktörleri ve quorum sensing (Virulence factors of Pseudomonas aeruginosa and quorum sensing). Türk Mikrobiyoloji Cemiyeti Dergisi, 38(1), 42-51.
  • Kozlova, E.V., Popov, V.L., Sha, J., Foltz, S.M., Erova, T.E., Agar, S.L, Horneman, A.J. & Chopra, A.K. (2008). Mutation in the S-ribosylhomocysteinase (luxS) gene involved in quorum sensing affects biofilm formation and virulence in a clinical isolate of Aeromonas hydrophila. Microbial Pathogenesis, 45(5–6), 343-354. DOI: 10.1016/j.micpath.2008.08.007
  • Mathewson, J.J. & Dupont, H.L. (1992). Aeromonas species: role as human pathogens, In: Remington, J.S., Swartz, M.N. (Ed.), Current Clinical Topics in Infectious Diseases, (pp 26-36), Vol12e. Cambridge: Blackwell Scientific.
  • McClean, K.H., Winson, M.K., Fish, L., Taylor, A., Chhabra, S.R., Camara, M., Daykin, M., Lamb, J.H., Swift, S., Bycroft, B.W., Stewart, G.S.A.B. & Williams, P. (1997). Quorum sensing and Chromobacterium violeceum: exploitation of violacein production for the detection of N-acylhomoserine lactones. Microbiology, 143, 3703-3711. DOI: 10.1099/00221287-143-12-3703
  • Moghaddam, M.M., Khodi, S. & Mirhosseini, A. (2014). Quorum Sensing in Bacteria and a Glance on Pseudomonas aeruginosa. Clinical Microbiology: Open Access, 3(4), ISSN: 2327-5073. DOI: 10.4172/2327-5073.1000156
  • Nijland, R. (2010). Bacteria can have a 'sense of smell'. Science Daily, Science News, 17 August 2010. <www.sciencedaily.com/releases/2010/08/100816095719.htm>.
  • Ohman, D.E., Cryz, S.J. & Iglewski, B.H. (1980). Isolation and characterization of a P. aeruginosa PAO mutant that produces altered elastase. Journal of Bacteriology, 142, 836- 842.
  • Pandey, A., Naik, M. & Dubey, S.K. (2010). Hemolysin, Protease, and EPS Producing Pathogenic Aeromonas hydrophila Strain An4 Shows Antibacterial Activity against Marine Bacterial Fish Pathogens. Journal of Marine Biology, Article ID 563205, 9 pages. DOI: 10.1155/2010/563205
  • Rahim, Z., Sanyal, S.C., Aziz, K.M., Huq, M.I. & Chowdhury, A.A. (1984). Isolation of enterotoxigenic, hemolytic, and antibiotic-resistant Aeromonas hydrophila strains from infected fish in Bangladesh. Applied and Environmental Microbiology, 48(4), 865-867.
  • Salyers, A.A. & Whitt, D.D. (1994). Bacterial pathogenesis: a molecular approach. ASM Press, Washington, D. C., USA, pp. 260-268.
  • Saraçlı, M.A. (2006). "Quorum sensing": mikroorganizmalar iletişim mi kuruyor? Gülhane Tıp Dergisi, 48(4), 244-250.
  • Schwenteit, J., Gram, L., Nielsen, K.F., Fridjonsson, O.H., Bornscheuer, U.T., Givskov, M. & Gudmundsdottir B.K. (2011). Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence. Veterinary Microbiology, 147, 389-397. DOI: 10.1016/j.vetmic.2010.07.020
  • Siegmund, I. & Wagner, F. (1991). New methods for detecting rhamnolipids excreted by Pseudomonas species during growth in mineral agar. BioTechniques, 5, 265-268.
  • Swift, S., Lynch, M.J., Fish, L., Kirke, D.F., Tomas, J.M., Stewart, G.S.A.B. & Williams, P. (1999). Quorum Sensing-Dependent Regulation and Blockade of Exoprotease Production in Aeromonas hydrophila. Infection and Immunity, 67(10), 5192-5199.
  • Swift, S., Karlyshev, A.V., Fish, L., Durant, E. L., Winson, M. K., Chhabra, S. R., Williams, P., Macintyre, S., & Stewart, G. S. A. B. (1997). Quorum Sensing in Aeromonas hydrophila and Aeromonas salmonicida: Identification of the LuxRI Homologs AhyRI and AsaRI and Their Cognate N-Acylhomoserine Lactone Signal Molecules. Journal of Bacteriology, 179(17), 5271-5281. DOI: 10.1128/jb.179.17.5271-5281.1997
  • Timur, G., & Timur, M. (2003). Balık Hastalıkları, İstanbul Üniversitesi Su Ürünleri Fakültesi, Yayın No: 5, s.538, İstanbul.
  • Ulusoy, S. (2007). Yoğun Bakım Ünitelerinden İzole Edilen Pseudomonas aeruginosa Suşlarında N-Açil Homoserin Lakton Üretiminin Araştırılması. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, 100s, Isparta.
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Details

Primary Language Turkish
Subjects Biology
Journal Section Articles
Authors

Nurdan FİLİK (Primary Author)
ısparta uygulamalı bilimler üniversitesi
0000-0003-4376-7298
Türkiye


Ayşegül KUBİLAY
ısparta uygulamalı bilimler üniversitesi
0000-0002-6043-2599
Türkiye

Thanks Bu araştırma makalesi Yüksek Lisans tezinden özetlenmiştir.
Publication Date March 15, 2020
Application Date March 27, 2019
Acceptance Date September 6, 2019
Published in Issue Year 2020, Volume 37, Issue 1

Cite

APA Filik, N. & Kubilay, A. (2020). Aeromonas hydrophila balık patojeninde biyosensör suşlar aracılığıyla çevreyi algılama sisteminin tespiti ve virülens faktörleri . Ege Journal of Fisheries and Aquatic Sciences , 37 (1) , 29-36 . DOI: 10.12714/egejfas.37.1.04