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Determination of oxidative stress in scaly carp (Cyprinus carpio Linnaeus, 1758) exposed to deltamethrin in different water temperature

Year 2017, Volume 34, Issue 3, 281 - 286, 26.09.2017
https://doi.org/10.12714/egejfas.2017.34.3.06

Abstract

In this study, it was aim to investigate oxidative stress in scaly carp (Cyprinus carpio) exposed to delthamethrin in different temperatures. For this purpose, fish were stocked to six different glass aquarium which was adjusted to 23 °C water temperature with adjustable thermostat heaters. After fifteen days adaptation period, temperatures of two aquarium were decreased to 18 °C, while temperatures of the other two were increased to 28 °C. These groups were exposed to 0.036 μg/L concentration of deltamethrin for 14 days. At the end of the experimental period, the liver and gills were taken. Malondialdehyde (MDA) level as an indicator of lipid peroxidation, reduced glutathione (GSH) level and glutathione S-transferase (GST) activity were determined in these samples. The MDA levels were increased in the liver and gill of the groups at 18 °C and 28 °C when compared to the control group. The liver and gill MDA levels of the groups treated deltamethrin simultaneously with the change in temperature were higher than the groups at 18 °C and 28 °C. This increase was also observed in the group treated deltamethrin at 23 ºC. The GSH levels and GST activities were decreased in the liver and gill of the groups at 18 °C and 28 °C when compared to the control group. The GSH level and GST activity of the groups treated deltamethrin simultaneously with the change in temperature were lower than the groups at 18 °C and 28 °C. This increase was also observed in the group treated deltamethrin at 23 ºC.

References

  • Atamanalp, M. & Yanık, T. (2001). Pestisitlerin Cyprinidae’lere toksik etkileri. Ege Üniversitesi Su Ürünleri Dergisi, 18:555-563.
  • Çelikkale, M.S. (1994). İçsu Balıkları ve Yetiştiriciliği (Cilt II). Trabzon: Karadeniz Teknik Üniversitesi Sürmene Deniz Bilimleri Fakültesi Yayınları.
  • Dikel, S. (2009). Su sıcaklığının balık yetiştiriciliğine etkisi. Alınteri, 16(B): 42-49.
  • Ellman, G.L. (1959). Tissue sulphydryl groups. Archives of Biochemistry and Biophysics, 82:70-77. doi: 10.1016/0003-9861(59)90090-6
  • Habig, W.H., Pabst, M.J. & Jakoby, W.B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry, 249:7130-7139.
  • Hamed, H.S. (2016). Ameliorative effects of Spirulina platensis on deltamethrin-induced biochemical alterations and oxidative stress in the African catfish; Clarias gariepinus. Open Journal of Marine Science, 6:1-10.
  • Hamed, R.R., Farid, N.M., Elowa, S.H.E. & Abdalla, A.M. (2003). Glutathione related enzyme levels of freshwater fish as bioindicators of pollution. The Environmentalist, 23:313–322.
  • Hayes, J.D. & McLellan, L.I. (1999). Glutathione and glutathione-dependent enzymes represent a coordinately regulated defence against oxidative stress. Free Radical Research, 31:273-300.
  • Hwang, D.F. & Lin, T.K. (2002). Effect of temperature on dietary vitamin C requirement and lipid in common carp. Comparative Biochemistry and Physiology B, 131(1):1-7. doi: 10.1016/S1096-4959(01)00449-3
  • Kalaycı, Ş. (2010). SPSS Uygulamalı Çok Değişkenli İstatistik Teknikleri. Ankara: Asil Yayınları.
  • Karasu Benli, A.Ç. & Gülen, Z. (2009). Fenitrothion’un etkisinde bırakılan tilapia’da (Oreochromis niloticus L.) sekonder stres indikatörleri hematokrit ve plazma glukoz seviyesinin değişimi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 19:19-22.
  • Kaur, M., Atif, F., Ali, M., Rehman, H. & Raisuddin, S. (2005). Heat stress-induced alterations of antioxidants in the freshwater fish Channa punctata Bloch. Journal of Fish Biology, 67:1653–1665. doi: 10.1111/j.1095-8649.2005.00872.x
  • Kaur, M., Atif, F., Rizwan, A.A., Ahmad, F. & Raisuddin, S. (2011). The interactive effect of elevated temperature on deltamethrin-induced biochemical stress responses in Channa punctata Bloch. Chemico-Biological Interactions, 193:216-224. doi:10.1016/j.cbi.2011.06.011
  • Kaya, E. (2005). Klorprifos ve deltamethrin’in kan ve beyin lipid peroksidasyon ve antioksidan enzim aktivitelerine etkileri. Yüksek Lisans Tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Isparta.
  • Kocaçalışkan, İ. & Bingöl, N.A. (2008). Biyoistatistik. Ankara: Nobel Yayınları.
  • Köprücü, K. & Aydın, R. (2004). The toxic effects of pyrethroid deltamethrin on the common carp (Cyprinus carpio L.) embryos and larvae. Pesticide Biochemistry and Physiology, 80:47–53. doi: 10.1016/j.pestbp.2004.05.004
  • Lloyd, R. (1992). Pollution and Freshwater Fish. Cornwall: A Buckland Foundation Book, Fishing News Books.
  • Lowry, O.H., Rosenberough, N.J., Farr, A.L. & Randal, R.J. (1951). Protein measurement with folinphenol reagent. Journal of Biochemistry, 193:265-275.
  • Mişe Yonar, S., Yonar, M.E., Sağlam, N. & Silici, S. (2013). Farklı su sıcaklıklarında tutulmuş pullu sazan (Cyprinus carpio carpio linnaeus, 1758)’nın karaciğer ve böbreğindeki bazı antioksidan parametreler üzerine propolisin etkisi. Menba, Kastomunu Üniversitesi Su Ürünleri Fakültesi Dergisi, 1:11-16.
  • Morales, A.E., Pèrez-Jimènez, A., Hidalgo, M.C., Abellán, E. & Gabriel C.G. (2004). Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comporative Biochemistry and Physiology C, 139(1-3):153-161. doi: 10.1016/j.cca.2004.10.008
  • Özcan Oruç, E. & Usta, D. (2007). Evaluation of oxidative stress responses and neurotoxicity potential of diazinon in different tissues of Cyprinus carpio. Environmental Toxicology and Pharmacology, 23(1):48-55. doi: 10.1016/j.etap.2006.06.005
  • Parihar, M.S. Javeri, T., Hemnani, T., Dubey, A.K. & Prakash, P. (1997). Responses of superoxide dismutase, glutathione peroxidase and reduced glutathione antioxidant defenses in gills of the fresh water catfish (Heteropneustes fossilis) to short-term elevated temperature. Journal of Thermal Biology, 22:151–156. doi: 10.1016/S0306-4565(97)00006-5
  • Placer, Z.A., Cushman, L. & Johnson, B.C. (1966). Estimation of products of lipid peroxidation (Malonyl dialdehyde) in biological fluids. Analytical Biochemistry, 16:359–364.
  • Roche, H. & Boge, G. (1996). Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication. Marine Environmental Research, 41:27–43. doi: 10.1016/0141-1136(95)00015-1
  • Sayeed, I., Parvez, S. Pandey, S., Bin-Hafeez, B., Haque, R. & Raisuddin, S. (2003). Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicology and Environmental Safety, 56:295–301. doi: 10.1016/S0147-6513(03)00009-5
  • Sharma, D.K. & Ansari, B.A. (2013). Effects of deltamethrin on CAT, LPO and GSH in tissues of zebrafish Daniro rerio. Research Journal of Environmental Toxicology, 7(1):38-46.
  • Sümbüloğlu, K. (1998). Biyoistatistik. Ankara: Hatipoğlu Yayınevi.
  • Vinagre, C., Madeira, D., Narciso, L., Cabral, H.N. & Diniz, M. (2012). Effect of temperature on oxidative stress in fish: Lipid peroxidation and catalase activity in the muscle of juvenile seabass, Dicentrarchus labrax. Ecological Indicators, 23:274-279. doi: 10.1016/j.ecolind.2012.04.009
  • Welker, T.L. & Congleton, J.L. (2004). Oxidative stress in juvenile chinook salmon, Oncorhynchus tshawytscha (Walbaum). Aquaculture Research, 35:881-887. doi: 10.1111/j.1365-2109.2004.01080.x
  • Yonar, M.E. and Sakin, F., 2011. Ameliorative effect of lycopene on antioxidant status in Cyprinus carpio during piretiroit deltamethrin exposure. Pesticide Biochemistry and Physiology, 99:226-231. doi: 10.1016/j.pestbp.2010.12.008

Farklı su sıcaklıklarında deltamethrin uygulanan pullu sazan (Cyprinus carpio Linnaeus, 1758)’da oksidatif stresin belirlenmesi

Year 2017, Volume 34, Issue 3, 281 - 286, 26.09.2017
https://doi.org/10.12714/egejfas.2017.34.3.06

Abstract

Bu çalışmada, farklı sıcaklıklarda deltamethrin uygulanmış pullu sazanda oksidatif stresin araştırılması amaçlanmıştır. Bu amaçla balıklar, termostatlı ısıtıcılarla su sıcaklıkları 23 oC’ye ayarlanmış 6 farklı cam akvaryumda stoklanmıştır. 15 günlük adaptasyon süresinden sonra, balıkların yerleştirildiği akvaryumlardan ikisinin su sıcaklığı 18 oC’ye düşürülürken, diğer ikisinin ise 28 oC’ye yükseltilmiştir. Bu gruplara 0.036 μg/L konsantrasyonunda deltamethrin 14 gün süreyle verilmiştir. Deneme sonunda balıklardan karaciğer ve solungaç örnekleri alınmıştır. Bu örneklerde lipit peroksidasyonun bir göstergesi olarak malondialdehit (MDA) düzeyi, redükte glutatyon (GSH) düzeyi ile glutatyon S-transferaz (GST) aktivitesi belirlenmiştir. Kontrol grubuyla kıyaslandığında, 18 ºC ve 28 ºC'deki grupların karaciğer ve solungaçlarındaki MDA düzeyinin arttığı görülmüştür. Sıcaklıktaki değişimle eşzamanlı olarak deltamethrin uygulanan gruplarda MDA düzeyleri 18 ºC ve 28 ºC'deki gruplardan daha yüksek bulunmuştur. Bu artış sıcaklığın 23 ºC' de kaldığı ve deltamethrin uygulanan grupta da gözlemlenmiştir.   Kontrol grubuyla kıyaslandığında, 18 ºC ve 28 ºC'deki grupların karaciğer ve solungaçlarındaki GSH düzeyleri ve GST aktivitelerinin azaldığı belirlenmiştir. Sıcaklıktaki değişimle eşzamanlı olarak deltamethrin uygulanan gruplarda GSH düzeyleri ve GST aktiviteleri 18 ºC ve 28 ºC'deki gruplardan daha düşük bulunmuştur. Bu azalma sıcaklığın 23 ºC' de kaldığı ve deltamethrin uygulanan grupta da görülmüştür.

References

  • Atamanalp, M. & Yanık, T. (2001). Pestisitlerin Cyprinidae’lere toksik etkileri. Ege Üniversitesi Su Ürünleri Dergisi, 18:555-563.
  • Çelikkale, M.S. (1994). İçsu Balıkları ve Yetiştiriciliği (Cilt II). Trabzon: Karadeniz Teknik Üniversitesi Sürmene Deniz Bilimleri Fakültesi Yayınları.
  • Dikel, S. (2009). Su sıcaklığının balık yetiştiriciliğine etkisi. Alınteri, 16(B): 42-49.
  • Ellman, G.L. (1959). Tissue sulphydryl groups. Archives of Biochemistry and Biophysics, 82:70-77. doi: 10.1016/0003-9861(59)90090-6
  • Habig, W.H., Pabst, M.J. & Jakoby, W.B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry, 249:7130-7139.
  • Hamed, H.S. (2016). Ameliorative effects of Spirulina platensis on deltamethrin-induced biochemical alterations and oxidative stress in the African catfish; Clarias gariepinus. Open Journal of Marine Science, 6:1-10.
  • Hamed, R.R., Farid, N.M., Elowa, S.H.E. & Abdalla, A.M. (2003). Glutathione related enzyme levels of freshwater fish as bioindicators of pollution. The Environmentalist, 23:313–322.
  • Hayes, J.D. & McLellan, L.I. (1999). Glutathione and glutathione-dependent enzymes represent a coordinately regulated defence against oxidative stress. Free Radical Research, 31:273-300.
  • Hwang, D.F. & Lin, T.K. (2002). Effect of temperature on dietary vitamin C requirement and lipid in common carp. Comparative Biochemistry and Physiology B, 131(1):1-7. doi: 10.1016/S1096-4959(01)00449-3
  • Kalaycı, Ş. (2010). SPSS Uygulamalı Çok Değişkenli İstatistik Teknikleri. Ankara: Asil Yayınları.
  • Karasu Benli, A.Ç. & Gülen, Z. (2009). Fenitrothion’un etkisinde bırakılan tilapia’da (Oreochromis niloticus L.) sekonder stres indikatörleri hematokrit ve plazma glukoz seviyesinin değişimi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 19:19-22.
  • Kaur, M., Atif, F., Ali, M., Rehman, H. & Raisuddin, S. (2005). Heat stress-induced alterations of antioxidants in the freshwater fish Channa punctata Bloch. Journal of Fish Biology, 67:1653–1665. doi: 10.1111/j.1095-8649.2005.00872.x
  • Kaur, M., Atif, F., Rizwan, A.A., Ahmad, F. & Raisuddin, S. (2011). The interactive effect of elevated temperature on deltamethrin-induced biochemical stress responses in Channa punctata Bloch. Chemico-Biological Interactions, 193:216-224. doi:10.1016/j.cbi.2011.06.011
  • Kaya, E. (2005). Klorprifos ve deltamethrin’in kan ve beyin lipid peroksidasyon ve antioksidan enzim aktivitelerine etkileri. Yüksek Lisans Tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Isparta.
  • Kocaçalışkan, İ. & Bingöl, N.A. (2008). Biyoistatistik. Ankara: Nobel Yayınları.
  • Köprücü, K. & Aydın, R. (2004). The toxic effects of pyrethroid deltamethrin on the common carp (Cyprinus carpio L.) embryos and larvae. Pesticide Biochemistry and Physiology, 80:47–53. doi: 10.1016/j.pestbp.2004.05.004
  • Lloyd, R. (1992). Pollution and Freshwater Fish. Cornwall: A Buckland Foundation Book, Fishing News Books.
  • Lowry, O.H., Rosenberough, N.J., Farr, A.L. & Randal, R.J. (1951). Protein measurement with folinphenol reagent. Journal of Biochemistry, 193:265-275.
  • Mişe Yonar, S., Yonar, M.E., Sağlam, N. & Silici, S. (2013). Farklı su sıcaklıklarında tutulmuş pullu sazan (Cyprinus carpio carpio linnaeus, 1758)’nın karaciğer ve böbreğindeki bazı antioksidan parametreler üzerine propolisin etkisi. Menba, Kastomunu Üniversitesi Su Ürünleri Fakültesi Dergisi, 1:11-16.
  • Morales, A.E., Pèrez-Jimènez, A., Hidalgo, M.C., Abellán, E. & Gabriel C.G. (2004). Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comporative Biochemistry and Physiology C, 139(1-3):153-161. doi: 10.1016/j.cca.2004.10.008
  • Özcan Oruç, E. & Usta, D. (2007). Evaluation of oxidative stress responses and neurotoxicity potential of diazinon in different tissues of Cyprinus carpio. Environmental Toxicology and Pharmacology, 23(1):48-55. doi: 10.1016/j.etap.2006.06.005
  • Parihar, M.S. Javeri, T., Hemnani, T., Dubey, A.K. & Prakash, P. (1997). Responses of superoxide dismutase, glutathione peroxidase and reduced glutathione antioxidant defenses in gills of the fresh water catfish (Heteropneustes fossilis) to short-term elevated temperature. Journal of Thermal Biology, 22:151–156. doi: 10.1016/S0306-4565(97)00006-5
  • Placer, Z.A., Cushman, L. & Johnson, B.C. (1966). Estimation of products of lipid peroxidation (Malonyl dialdehyde) in biological fluids. Analytical Biochemistry, 16:359–364.
  • Roche, H. & Boge, G. (1996). Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication. Marine Environmental Research, 41:27–43. doi: 10.1016/0141-1136(95)00015-1
  • Sayeed, I., Parvez, S. Pandey, S., Bin-Hafeez, B., Haque, R. & Raisuddin, S. (2003). Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicology and Environmental Safety, 56:295–301. doi: 10.1016/S0147-6513(03)00009-5
  • Sharma, D.K. & Ansari, B.A. (2013). Effects of deltamethrin on CAT, LPO and GSH in tissues of zebrafish Daniro rerio. Research Journal of Environmental Toxicology, 7(1):38-46.
  • Sümbüloğlu, K. (1998). Biyoistatistik. Ankara: Hatipoğlu Yayınevi.
  • Vinagre, C., Madeira, D., Narciso, L., Cabral, H.N. & Diniz, M. (2012). Effect of temperature on oxidative stress in fish: Lipid peroxidation and catalase activity in the muscle of juvenile seabass, Dicentrarchus labrax. Ecological Indicators, 23:274-279. doi: 10.1016/j.ecolind.2012.04.009
  • Welker, T.L. & Congleton, J.L. (2004). Oxidative stress in juvenile chinook salmon, Oncorhynchus tshawytscha (Walbaum). Aquaculture Research, 35:881-887. doi: 10.1111/j.1365-2109.2004.01080.x
  • Yonar, M.E. and Sakin, F., 2011. Ameliorative effect of lycopene on antioxidant status in Cyprinus carpio during piretiroit deltamethrin exposure. Pesticide Biochemistry and Physiology, 99:226-231. doi: 10.1016/j.pestbp.2010.12.008

Details

Subjects Science
Journal Section Articles
Authors

M. Enis Yonar
orcid.org/0000-0001-9519-4247
Türkiye


Ahmet Turan San This is me

Publication Date September 26, 2017
Application Date February 22, 2017
Acceptance Date May 26, 2017
Published in Issue Year 2017, Volume 34, Issue 3

Cite

APA Yonar, M. E. & San, A. T. (2017). Determination of oxidative stress in scaly carp (Cyprinus carpio Linnaeus, 1758) exposed to deltamethrin in different water temperature . Ege Journal of Fisheries and Aquatic Sciences , 34 (3) , 281-286 . DOI: 10.12714/egejfas.2017.34.3.06