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Alterations in the hematological parameters of rainbow trout (Oncorhynchus mykiss) injected with nonylphenol

Year 2016, Volume 33, Issue 2, 139 - 142, 15.05.2016
https://doi.org/10.12714/egejfas.2016.33.2.07

Abstract

Abstract: : This study was conducted to investigate the effect of nonylphenol (NP) on the hematological parameters of rainbow trout, Oncorhynchus mykiss, after 3 and 7 days of exposure. The fish were intraperitoneally injected with 5 and 50 mg/kg doses of NP. The red blood cell count decreased significantly with both doses of NP at 3 days post injection. The white blood cell values showed a significant increase at 3 days post injection with 50 mg/kg NP, and this increase was also observed with 5 mg/kg NP at 7 days post injection. At 3 days post injection, hemoglobin was seen at significantly low levels, while the packed cell volume was at higher values with both doses of NP. No significant differences were observed in the erythrocyte sedimentation rate. The mean corpuscular volume showed significant increases at 3 days post injection, the mean corpuscular hemoglobin was observed to decrease with 5 mg/kg NP at 7 days post injection, and the mean corpuscular hemoglobin concentration exhibited significantly lower values at 3 days post injection. In conclusion, this study indicated that the treatment concentrations of NP induced distinct hematological changes in rainbow trout.

Keywords: Nonylphenol, hematological parameters, rainbow trout

References

  • Adeyemo, O.K. (2005). Haematological and histopathological effects of Cassava mill effluent in Clarias gariepinus. African Journal Biomedical Research, 8(3): 179-183. doi:10.4314/ajbr.v8i3.35747
  • Arukwe, A., Förlin, L., & Goksoyr, A. (1997). Xenobiotic and steroid biotransformation enzymes in atlantic salmon (Salmo salar) liver treated with an estrogenic compound, 4-nonylphenol. Environmental Toxicology and Chemistry, 16(12): 2576-2583. doi:10.1002/etc.5620161220
  • Atamanalp, M., Aksakal, E., Kocaman, E.M., Uçar, A., Şişman, T., & Türkez, H. (2011). The alterations in the hematological parameters of rainbow trout, Oncorhynchus mykiss, exposed to cobalt chloride. Journal of the Faculty of Veterinary Medicine, Kafkas University, 17: 73-76. doi:10.9775/kvfd.2010.3393
  • Atamanalp, M., Angis, S., Oguzhan, P., & Aksakal, E. (2008). Alterations in hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to DDVP. The Israeli Journal of Aquaculture-Bamidgeh, 60(1): 9-12.
  • Blaxhall, P.C., & Daisley, K.W. (1973). Routine Haematological Methods for use Fish with Blood. Journal of Fish Biology, 5(6): 771-781. doi:10.1111/j.1095-8649.1973.tb04510.x
  • Blaxhall, P.C. (1972). The Haematological Assessment of the Health of Freshwater Fish. A Rewiev of Selected Literature. Journal of Fish Biology, 4(4): 593-604. doi:10.1111/j.1095-8649.1972.tb05704.x
  • Corsi, I., & Focardi, S. (2002). Nonylphenols in a lagoon environment: p-nonylphenol and nonylphenol ethoxylates in fish tissue. Bulletin of Environmental Contamination and Toxicology, 68(6): 908-914. doi:10.1007/s00128-002-0040-7
  • Gabriel, U.U., Obomanu, F.G., & Edori, O.S. (2009). Haematology, plasma enzymes and organ indices of Clarias gariepinus after intramuscular injection with aqueous leaves extracts of Lepidagathis alopecuroides. African Journal of Biochemistry Research, 3(9): 312-316.
  • Hughes, E.M., & Gallagher, E.P. (2004). Effects of 17-β estradiol and 4-nonylphenol on phase II electrophilic detoxification pathways in largemouth bass (Micropterus salmoides) liver. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 137(3): 237-247. doi:10.1016/j.cca.2004.01.006
  • Jobling, S., Nolan, M., Tyler, C.R., Brighty, G., & Sumpter, J.P. (1998). Widespread sexual disruption in wild fish. Environmental Science and Technology, 32(17): 2498-2506. doi:10.1021/es9710870
  • Kannan, K., Keith, T.L., Nalor, C.G., Staples, A., Synder, S.A., & Giesy, J.P. (2003). Nonylphenol and nonylphenol ethoxylates in fish, sediment, and water from the Kalamazoo River, Michigan. Archives of Environmental Contamination and Toxicology, 44(1): 77-82. doi:10.1007/s00244-002-1267-3
  • Keum, Y.H., Jee, J.H., Lee, O.H., Park, S.I., & Kang, J.C. (2005). In vivo effects of bisphenol A exposure on haematological parameters in Korean rockfish, Sebastes schlegeli. Journal of Fish Pathology, 18(3): 293-300.
  • Kinnberg, K., Korsgaard, B., Bjerregaard, P., & Jespersen, A. (2000). Effects of nonylphenol and 17β-estradiol on vitellogenin synthesis and testis morphology in male platyfish Xiphophorus maculates. The Journal of Experimental Biology, 203(2): 171-181.
  • Kocabatmaz, M., & Ekingen, G. (1984). Taking blood sample in different fish species and standardization of hematological methods. Doğa Bilim Dergisi, 8(2): 149-159.
  • Kumaran, S.S., Kavitha, C., Ramesh, M., & Grummt, T. (2011). Toxicity studies of nonylphenol and octylphenol: hormonal, hematological and biochemical effects in Clarias gariepinus. Journal of Applied Toxicology, 31(8): 752-761. doi:10.1002/jat.1629.
  • Lech, J.J., Lewis, S.K., & Ren, L. (1996). In vivo estrogenic activity of nonylphenol in rainbow trout. Fundamental and Applied Toxicology, 30(2): 229-232. doi:10.1093/toxsci/30.2.229
  • Li, M.H., & Wang, Z.R. (2005). Effect of nonylphenol on plasma vitellogenin of male adult guppies (Poecilia reticulata). Environmental Toxicology, 20(1): 53-59. doi:10.1002/tox.20077
  • Mousavi, S.E., & Yousefian, M. (2012). The alterations in the hematological parameters of endangered Caspian brown trout, Salmo trutta caspius, exposed to waterborne mercuric chloride. Asian Journal of Animal Sciences, 6(4): 154-163. doi:10.3923/ajas.2012.154.163
  • OECD. (1992). OECD guideline for testing of chemicals. Fish, acute toxicity test. The Organisation for Economic Co-operation and Development (OECD), 203: 1-9. doi:10.1787/9789264069961-en
  • Reddy, P.M., & Bashamoideen, M.D. (1989). Fenvalarate and Cypermethrin İnduced Changes in the Haematological Parameters of Cyprinus carpio. Acta Hydrochimica et Hydrobiologica, 17(1): 101-107. doi:10.1002/aheh.19890170116
  • Schalm, O.W., Jain, N.C., & Carrol, E.J. (1975). Veterinary Haematology. 3rd edn. Lea and Febiger (US).
  • Schwaiger, J., Spieser, O.H., Bauer, C., Ferling, H., Mallow, U., Kalbfus, W., & Negele, R.D. (2000). Chronic toxicity of nonylphenol and ethinylestradiol: haematological and histopathological effects in juvenile common carp (Cyprinus carpio). Aquatic Toxicology, 51(1): 69-78. doi:10.1016/S0166-445X(00)00098-9
  • Shao, B., Hu, J., Yang, M., An, W., & Tao, S. (2005). Nonylphenol and nonylphenol ethoxylates in river, drinking water, and fish tissues in the area of Chongqing, China. Archives of Environmental Contamination and Toxicology, 48(4): 467-473. doi:10.1007/s00244-003-0266-3
  • Svoboda, M., Luskova, V., Drastichova, J., & Zlabek, V. (2001). The effect of diazinon on haematological indices of common carp (Cyprinus carpio L.). Acta Veterinaria Brno., 70: 457-465. doi:10.2754/avb200170040457
  • Uguz, C., Iscan, M., Ergüven, A., Isgor, B., & Togan, I. (2003). The bioaccumulation of nonylphenol and its adverse effect on the liver of rainbow trout (Onchorynchus mykiss). Environmental Research, 92(3): 262-270. doi:10.1016/S0013-9351(03)00033-1
  • Vaccaro, E., Meucci, V., Intorre, L., Soldani, G., Di Bello, D., Longo, V., Gervasi, P.G., & Pretti, C. (2005). Effects of 17 β-estradiol, 4-nonylphenol and PCB 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (Dicentrarchus labrax). Aquatic Toxicology, 75(4): 293-305. doi:10.1016/j.aquatox.2005.08.009
  • Vazquez, G.R., & Nostro, F.L. (2014). Changes in hematological parameters of Cichlasoma dimerus (Teleostei, Perciformes) exposed to sublethal concentrations of 4-tert-octylphenol. Archives of Environmental Contamination and Toxicology, 66(3): 463-469.
  • doi:10.1007/s00244-014-9997-6
  • Vazquez-Duhalt, R., Marquez-Rocha, F., Ponce, E., Licea, A.F., & Viana, M.T. (2005). Nonylphenol, an integrated vision of a pollutant. Scientific review. Applied Ecology and Environmental Research, 4(1): 1-25. doi:10.15666/aeer/0401_001025
  • Villeneuve, D.L., Villalobos, S.A., Keith, T.L., Snyder, E.M., Fitzgerald, S.D., & Giesy, J.P. (2002). Effects of waterborne exposure to 4-nonylphenol on plasma sex steroid and vitellogenin concentrations in sexually mature male carp (Cyprinus carpio). Chemosphere, 47(1):15-28. doi:10.1016/S0045-6535(01)00212-0

Year 2016, Volume 33, Issue 2, 139 - 142, 15.05.2016
https://doi.org/10.12714/egejfas.2016.33.2.07

Abstract

Bu çalışma, nonilfenole (NP) 3 ile 7 gün süreyle maruz bırakılan gökkuşağı alabalığı (Oncorhynchus mykiss)’nın kan parametreleri üzerindeki etkisini araştırmak için yürütülmüştür. NP balıklara 5 ve 50 mg/kg dozunda intraperitonal olarak enjekte edilmiştir. Enjeksiyondan 3 gün sonra NP’nin her iki dozunda eritrosit sayısı önemli olarak azalmıştır. Lökosit sayısı enjeksiyondan 3 gün sonra 50 mg/kg NP’de önemli olarak artış göstermiş ve bu artış enjeksiyondan 7 gün sonra 5 mg/kg NP’de de gözlenmiştir. Enjeksiyondan 3 gün sonra NP’nin her iki dozunda hematokrit yükselirken hemoglobin önemli olarak daha düşük seviyelerde görülmüştür. Eritrosit sedimantasyon oranında önemli bir fark gözlenmemiştir. Enjeksiyondan 3 gün sonra ortalama eritrosit hacminin önemli olarak arttığı görülmüştür. Eritrosit başına düşen ortalama hemoglobin miktarı enjeksiyondan 7 gün sonra 5 mg/kg NP’de azaldığı gözlenmiştir. Eritrosit başına düşen ortalama hemoglobin konsantrasyonunun enjeksiyondan 3 gün sonra önemli olarak düştüğü gösterilmiştir. Sonuçta bu çalışma, NP’nin uygulama dozlarının gökkuşağı alabalığında belirgin hematolojik değişiklikleri uyardığını göstermiştir

References

  • Adeyemo, O.K. (2005). Haematological and histopathological effects of Cassava mill effluent in Clarias gariepinus. African Journal Biomedical Research, 8(3): 179-183. doi:10.4314/ajbr.v8i3.35747
  • Arukwe, A., Förlin, L., & Goksoyr, A. (1997). Xenobiotic and steroid biotransformation enzymes in atlantic salmon (Salmo salar) liver treated with an estrogenic compound, 4-nonylphenol. Environmental Toxicology and Chemistry, 16(12): 2576-2583. doi:10.1002/etc.5620161220
  • Atamanalp, M., Aksakal, E., Kocaman, E.M., Uçar, A., Şişman, T., & Türkez, H. (2011). The alterations in the hematological parameters of rainbow trout, Oncorhynchus mykiss, exposed to cobalt chloride. Journal of the Faculty of Veterinary Medicine, Kafkas University, 17: 73-76. doi:10.9775/kvfd.2010.3393
  • Atamanalp, M., Angis, S., Oguzhan, P., & Aksakal, E. (2008). Alterations in hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to DDVP. The Israeli Journal of Aquaculture-Bamidgeh, 60(1): 9-12.
  • Blaxhall, P.C., & Daisley, K.W. (1973). Routine Haematological Methods for use Fish with Blood. Journal of Fish Biology, 5(6): 771-781. doi:10.1111/j.1095-8649.1973.tb04510.x
  • Blaxhall, P.C. (1972). The Haematological Assessment of the Health of Freshwater Fish. A Rewiev of Selected Literature. Journal of Fish Biology, 4(4): 593-604. doi:10.1111/j.1095-8649.1972.tb05704.x
  • Corsi, I., & Focardi, S. (2002). Nonylphenols in a lagoon environment: p-nonylphenol and nonylphenol ethoxylates in fish tissue. Bulletin of Environmental Contamination and Toxicology, 68(6): 908-914. doi:10.1007/s00128-002-0040-7
  • Gabriel, U.U., Obomanu, F.G., & Edori, O.S. (2009). Haematology, plasma enzymes and organ indices of Clarias gariepinus after intramuscular injection with aqueous leaves extracts of Lepidagathis alopecuroides. African Journal of Biochemistry Research, 3(9): 312-316.
  • Hughes, E.M., & Gallagher, E.P. (2004). Effects of 17-β estradiol and 4-nonylphenol on phase II electrophilic detoxification pathways in largemouth bass (Micropterus salmoides) liver. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 137(3): 237-247. doi:10.1016/j.cca.2004.01.006
  • Jobling, S., Nolan, M., Tyler, C.R., Brighty, G., & Sumpter, J.P. (1998). Widespread sexual disruption in wild fish. Environmental Science and Technology, 32(17): 2498-2506. doi:10.1021/es9710870
  • Kannan, K., Keith, T.L., Nalor, C.G., Staples, A., Synder, S.A., & Giesy, J.P. (2003). Nonylphenol and nonylphenol ethoxylates in fish, sediment, and water from the Kalamazoo River, Michigan. Archives of Environmental Contamination and Toxicology, 44(1): 77-82. doi:10.1007/s00244-002-1267-3
  • Keum, Y.H., Jee, J.H., Lee, O.H., Park, S.I., & Kang, J.C. (2005). In vivo effects of bisphenol A exposure on haematological parameters in Korean rockfish, Sebastes schlegeli. Journal of Fish Pathology, 18(3): 293-300.
  • Kinnberg, K., Korsgaard, B., Bjerregaard, P., & Jespersen, A. (2000). Effects of nonylphenol and 17β-estradiol on vitellogenin synthesis and testis morphology in male platyfish Xiphophorus maculates. The Journal of Experimental Biology, 203(2): 171-181.
  • Kocabatmaz, M., & Ekingen, G. (1984). Taking blood sample in different fish species and standardization of hematological methods. Doğa Bilim Dergisi, 8(2): 149-159.
  • Kumaran, S.S., Kavitha, C., Ramesh, M., & Grummt, T. (2011). Toxicity studies of nonylphenol and octylphenol: hormonal, hematological and biochemical effects in Clarias gariepinus. Journal of Applied Toxicology, 31(8): 752-761. doi:10.1002/jat.1629.
  • Lech, J.J., Lewis, S.K., & Ren, L. (1996). In vivo estrogenic activity of nonylphenol in rainbow trout. Fundamental and Applied Toxicology, 30(2): 229-232. doi:10.1093/toxsci/30.2.229
  • Li, M.H., & Wang, Z.R. (2005). Effect of nonylphenol on plasma vitellogenin of male adult guppies (Poecilia reticulata). Environmental Toxicology, 20(1): 53-59. doi:10.1002/tox.20077
  • Mousavi, S.E., & Yousefian, M. (2012). The alterations in the hematological parameters of endangered Caspian brown trout, Salmo trutta caspius, exposed to waterborne mercuric chloride. Asian Journal of Animal Sciences, 6(4): 154-163. doi:10.3923/ajas.2012.154.163
  • OECD. (1992). OECD guideline for testing of chemicals. Fish, acute toxicity test. The Organisation for Economic Co-operation and Development (OECD), 203: 1-9. doi:10.1787/9789264069961-en
  • Reddy, P.M., & Bashamoideen, M.D. (1989). Fenvalarate and Cypermethrin İnduced Changes in the Haematological Parameters of Cyprinus carpio. Acta Hydrochimica et Hydrobiologica, 17(1): 101-107. doi:10.1002/aheh.19890170116
  • Schalm, O.W., Jain, N.C., & Carrol, E.J. (1975). Veterinary Haematology. 3rd edn. Lea and Febiger (US).
  • Schwaiger, J., Spieser, O.H., Bauer, C., Ferling, H., Mallow, U., Kalbfus, W., & Negele, R.D. (2000). Chronic toxicity of nonylphenol and ethinylestradiol: haematological and histopathological effects in juvenile common carp (Cyprinus carpio). Aquatic Toxicology, 51(1): 69-78. doi:10.1016/S0166-445X(00)00098-9
  • Shao, B., Hu, J., Yang, M., An, W., & Tao, S. (2005). Nonylphenol and nonylphenol ethoxylates in river, drinking water, and fish tissues in the area of Chongqing, China. Archives of Environmental Contamination and Toxicology, 48(4): 467-473. doi:10.1007/s00244-003-0266-3
  • Svoboda, M., Luskova, V., Drastichova, J., & Zlabek, V. (2001). The effect of diazinon on haematological indices of common carp (Cyprinus carpio L.). Acta Veterinaria Brno., 70: 457-465. doi:10.2754/avb200170040457
  • Uguz, C., Iscan, M., Ergüven, A., Isgor, B., & Togan, I. (2003). The bioaccumulation of nonylphenol and its adverse effect on the liver of rainbow trout (Onchorynchus mykiss). Environmental Research, 92(3): 262-270. doi:10.1016/S0013-9351(03)00033-1
  • Vaccaro, E., Meucci, V., Intorre, L., Soldani, G., Di Bello, D., Longo, V., Gervasi, P.G., & Pretti, C. (2005). Effects of 17 β-estradiol, 4-nonylphenol and PCB 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (Dicentrarchus labrax). Aquatic Toxicology, 75(4): 293-305. doi:10.1016/j.aquatox.2005.08.009
  • Vazquez, G.R., & Nostro, F.L. (2014). Changes in hematological parameters of Cichlasoma dimerus (Teleostei, Perciformes) exposed to sublethal concentrations of 4-tert-octylphenol. Archives of Environmental Contamination and Toxicology, 66(3): 463-469.
  • doi:10.1007/s00244-014-9997-6
  • Vazquez-Duhalt, R., Marquez-Rocha, F., Ponce, E., Licea, A.F., & Viana, M.T. (2005). Nonylphenol, an integrated vision of a pollutant. Scientific review. Applied Ecology and Environmental Research, 4(1): 1-25. doi:10.15666/aeer/0401_001025
  • Villeneuve, D.L., Villalobos, S.A., Keith, T.L., Snyder, E.M., Fitzgerald, S.D., & Giesy, J.P. (2002). Effects of waterborne exposure to 4-nonylphenol on plasma sex steroid and vitellogenin concentrations in sexually mature male carp (Cyprinus carpio). Chemosphere, 47(1):15-28. doi:10.1016/S0045-6535(01)00212-0

Details

Journal Section Articles
Authors

Ertuğrul KANKAYA This is me


Burak Kaptaner This is me

Publication Date May 15, 2016
Application Date May 15, 2016
Acceptance Date
Published in Issue Year 2016, Volume 33, Issue 2

Cite

APA Kankaya, E. & Kaptaner, B. (2016). Alterations in the hematological parameters of rainbow trout (Oncorhynchus mykiss) injected with nonylphenol . Ege Journal of Fisheries and Aquatic Sciences , 33 (2) , 139-142 . DOI: 10.12714/egejfas.2016.33.2.07