Year 2016, Volume 33 , Issue 2, Pages 113 - 120 2016-05-15

Alireza Mirvaghefi* • Mohsen Ali • Hadi Poorbagher
Effects of vitamin C on oxidative stress parameters in rainbow trout exposed to diazinon

Alireza MİRVAGHEFİ [1] , Mohsen ALİ [2] , Hadi POORBAGHER [3]

Abstract: : Diazinon (Organophosphorus insecticide) can cause reactive oxidative stresses during metabolism of aquatic organisms. The study presented here aimed to investigate the antioxidant effect of vitamin C on antioxidant enzyme activities and lipid peroxidation in rainbow trout exposed to subchronic dosage diazinon. A total number of 360 Oncorhynchus mykiss were allocated into four treatment groups (with three replicates): control; diazinon (0.1 mg L 1); vitamin C (300 mg kg 1 in diet) plus diazinon (0.1 mg L-1), and vitamin C (1000 mg kg-1 in diet) plus diazinon (0.1 mg L-1). After two and four weeks, blood samples were collected and superoxide dismutase, catalase enzymes, total antioxidant capacity and malondialdehyte index were measured. The specimens exposed exclusively to diazinon showed a significant decrease in superoxide dismutase and total antioxidant capacity activities and also an increase in catalase activity and malondialdehyde index compared to those of the control group. The changes in oxidative stress parameters may be related to destructive free oxiradicals such as superoxide and hydroxyl radicals produced in diazinon metabolism. In conclusion, vitamin C moderated activity of the antioxidant enzyme, increased the total antioxidant capacity, and decreased cellular damages caused by destructive lipid peroxidation process in presence of diazinon.

Keywords: Diazinon, antioxidant, vitamin C, enzyme, malondialdehyde, catalase
Diazinon, antioxidant, vitamin C, enzyme, malondialdehyde, catalase
  • Abdou, H.M. & El-Mazoudy, R.H. (2007). Myotoxic and hyperlipidemic effects of diazinon in female rats. Journal of Magnetic Resonance Imaging, 28:292-298. doi: 10.1016/j.pestbp.2009.11.007
  • Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105:121-126. doi: 10.1016/s0076-6879(84)05016-3
  • Akturk, O., Demirin, H., Sutcu, R., Yilmaz, N., Koylu, H. & Altuntas, I. (2006). The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C. Cell Biology and Toxicology, 22:455-461. doi: 10.1007/s10565-006-0138-5
  • Ali, M., Mirvaghefi, A. & Asadi, F. (2015). Effects of vitamin E, selenium and vitamin C on various biomarkers following oxidative stress caused by diazinon exposure in rainbow trout. Ege Journal of Fisheries and Aquatic Sciences, 32(3):151-158
  • Amirkabirian, N., Teimouri, F., Esmaily, H., Mohammadirad, A., Aliahmadi, A. & Abdollahi, M. (2007). Protection by pentoxifylline of diazinon-induced toxic stress in rat liver and muscle. Toxicology Mechanisms and Methods, 17:215-221. doi: 10.1080/15376510600943783
  • Arthur, JW., Zischke, J.A., Allen, K.N. & Hermanutz R.O. (1983). Effects of diazinon on macroinvertebrates and insect emergence in outdoor experimental channels. Aquatic Toxicology, 4:283-301. doi:10.1016/0166-445x(83)90023-1
  • Bailey, H.C., Deanovic, L., Reyes, E., Kimball, T., Larson, K., Cortright, K., Connor, V. & Hinton, D.E. (2000). Diazinon and chlorpyrifos in urban waterways in northern California, USA. Environmental Toxicology & Chemistry, 19:82-87. doi:10/1002/etc.5620190109
  • Banaee, M., Sureda, A., Mirvaghefi, A.R. & Ahmadi, K. (2011). Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and Physiology, 99:1-6. doi: 10.1016/pestbp.2010.09.001
  • Benzi, I.F.F., Chung, W.Y. & Strain, J.J. (1999). Antioxidant (reducing) efficiency of ascorbate in plasma is not affected by concentration. The Journal of Nutritional Biochemistry,10:146-150. doi: 10.1016/s0955-2863(98)00084-9
  • Bigard, A.X. (2001). Lesions musculaires induites par l’exercice et surentrainement. Science & Sports,16:204-215. doi: 10.1016/s0765-1597(00)00037-x
  • Buettner, G.R. & Moseley, P.L. (1993). EPR spin trapping of free radicals produced by bleomycin and ascorbate. Free radical research communications, 19: S89-S93. doi:10.3109/10715769309056s89
  • Castillo, C., Hernandez, J., Valverde, I., Pereira, V., Sotillo, J., Alonso, M.L. & Benedito, J.L. (2006). Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Research in Veterinary Science, 80:133-139. doi: 10.1016/j.rvsc.2005.06.003
  • Das, K.C., Lewis-Molock, Y. & White, C.W. (1997). Elevation of manganese superoxide dismutase gene expression by thioredoxin. American Journal of Respiratory Cell and Molecular Biology, 17:713-726. doi: 10.1165.ajrcmb.17.6.2809
  • Deshpande, S.S., Deshpande, U.S. & Salunkhe, D.K. (1996). Nutritional and health aspects of food antioxidants. In: Madhavi DL, Deshpande SS, Salunkhe DK (EDs.) Food antioxidants, technological, toxicological, and health perspectives, pp 361-470. New York: Marcel Dekker.
  • De Zwart, L., Meerman, J., Commandeur, J. & Vermeulen, N. (1999). Biomarkers of free radical damage applications in experimental animals and in humans. Free Radical Biology and Medicine, 26: 202-226. doi: 10.1016/s0891-5849(98)00196-8
  • Doba, T., Burton, G.W. & Ingold, K.U. (1985). Antioxidant and co-antioxidant activity of vitamin C. The effect of vitamin C, either alone or in the presence of vitamin E or a water-soluble vitamin E analogue, upon the peroxidation of aqueous multilamellar phospholipid liposomes. Biochimica et Biophysica Acta, 9: 298-303. doi: 10.1016/0005-2760(85)90285-1
  • Durmaz, H., Sevgiler, Y., Uner, N. (2006). Tissue-specific antioxidative and neurotoxic responses to diazinon in Oreochromis niloticus. Pesticide Biochemistry and Physiology, 3:215-226. doi: 1016/j.pestbp.2005.07.004
  • Eisler, R. (1998). Diazinon hazards to fish, wildlife and invertebrates: a synoptic review, Biological report 85, U.S. Fish and Wildlife service, Washington, DC.
  • El-Gendy, K.S., Aly, N.M., Mahmoud, F.H., Kenawy, A. & El-Sebae, A.H. (2010). The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid. Food and Chemical Toxicology, 48:215-221. doi: 1016/j.fct.2009.10.003
  • El-Gharieb, M.A., El-Masry, T.A., Emara, A.M., Mohammed, A. & Hashem, M.A. (2010). Potential hepatoprotective effects of vitamin E and Nigella sativa oil on hepatotoxicity induced by chronic exposure to malathion in human and male albino rats. Toxicological & Environmental Chemistry, 92:391-407. doi:10.1080/02772240902955719
  • Fabiana, G.F., Pilarski EM, Onaka, F.R.D, Moraes, M.L. & Martins, M.L. (2007). Hematology of Piaractus mesopotamicus fed diets supplemented with vitamins C and E, challenged by Aeromonas hydrophila. Aquaculture, 271:39-46. doi: 10.1016/j.aquaculture.2007.06.021
  • Frei, B., Stocker, R. & Ames, B.N. (1988). Antioxidant defenses and lipid peroxidation in human blood plasma. Proceedings of the National Academy of Sciences United States of America, 85: 9748-9752. doi: 10.1073/pnas.85.24.9748
  • Fujii Y. & Asaka, S. (1982). Metabolism of diazinon and diazoxon in fish liver preparations. The Bulletin of Environmental Contamination and Toxicology, 29:455-460. doi: 10.1007/BF01605611
  • Geetha, B.V., Navasakthi, R. & Padmini, E. (2010). Investigation of antioxidant capacity and phytochemical composition of Sun Chlorella- an in vitro study. Aquaculture Research & Development, 1:104. doi: 10.4172/2155-9546.1000104
  • Gokalp, O., Buyukvanli, B., Cicek, E., Ozer, M.K., Koyu, A., Altuntas, I. & Koylu, H. (2005). The effects of diazinon on pancreatic damage and ameliorating role of vitamins E and C. Pesticide Biochemistry and Physiology, 81: 123-128. doi: 10.1016/j.pestbp.2004.11.001
  • Goth, L. (1991). A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196:143-152. doi: 10.1016/0009-8981(91)90067-M
  • Halliwell, B. & Gutteridge, J.M.C. (1984). Oxygen toxicity, oxygen radicals, transition metals and disease. Biochemical Journal, J.219:1-14. doi: 10.1042/bj20140006
  • Hazarika, A., Sarkar, S.N, Hajare, S., Kataria, M. & Malik, J.K. (2003). Influence of malathion pretreatment on the toxicity of anilofos in male rats: a biochemical interaction study. Toxicology 185:1-8. doi: 10.1016/s0300-483x(02)00574-7
  • Isik, I. & Celik, I. (2008). Acute effects of methyl parathion and diazinon as inducers for oxidative stress on certain biomarkers in various tissues of rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and Physiology, 92:38-42. doi: 10.1016/j.pestbp.2008.06.001
  • Jakeman, P. & Maxwell, S. (1993). Effect of antioxidant vitamin supplementation on muscle function after eccentric exercise. European Journal of Applied Physiology and Occupational Physiology, 67: 426-430. doi: 10.1007/bf00376459
  • Jarvinen, A.W. & Tanner, D.K. (1982). Toxicity of selected controlled release and corresponding unformulated technical grade pesticides to the fathead minnow (Pimephales promelas). Environmental Pollution, 27: 179-195. doi: 10.1016/01471(82)90024-1
  • Jialal, I., Vega, G.I. & Grundy, S.M. (1990). Physiological levels of ascorbate inhibit the active modification of low density lipoprotein. Atherosclerosis, 82: 185-191. doi: 10.1016/0021-9150(90)90039-L
  • Kadry, S.M., Marzouk, M.S, Amer, A.F., Hanna, M.I., Azmy, A.H. & Hamed, H.S. (2012). Vitamin E as antioxidant in female african catfish (Clarias gariepinus) exposed to chronic toxicity of atrazine. Egyptian Journal of Aquatic Biology & Fisheries,16:83-98. doi: 10.1016/s0013-9351 (02)0006-2
  • Karaoz, E, Gultekin, F., Akdogan, M., Oncu, M. & Gokcimen, A. (2002). Protective role of melatonin and a combination of vitamin C and E on lung toxicity induced by chlorpyrifos-ethyl in rats. Experimental and Toxicologic Pathology, 54: 97-108. doi: 10.1078/0940-2993-00236
  • Keizer, J., D'Agostino, G., Nagel, R., Volpe, T., Gnemi, P. & Vittozzi, L. (1995). Enzymological differences of AChE and diazinon hepatic metabolism: correlation of in vitro data with the selective toxicity of diazinon to fish species. Science of the Total Environment, 171: 213-220. doi: 10.1016/0049-9697(95)04687-0
  • Kohen, R., Vellaichamy, E. & Hrbac, J. (2000). Quantification of the overall reactive oxygen species scavenging capacity of biolog-ical fluids and tissues. Free Radical Biology and Medicine, 28, 871-879. doi:10.1016/s0891-5849(00)00191-X
  • Koprucu, S.S., Koprucu, K., Ural, M.S, Ispir, U. & Pala, M. (2006). Acute toxicity of organophosphorous pesticide diazinon and its effects on behavior and some hematological parameters of fingerling European catfish (Silurus glanis L.). Pesticide Biochemistry and Physiology, 86: 99-105. doi: 10.1016/j.pestbp.2006.02.001
  • Koracevic, D., Koracevic, G., Djordjevic, V., Andrejevic, S. & Cosic, V. (2001). Method for the measurement of antioxidant activity in human fluids. Journal of Clinical Pathology, 54:356-361. doi: 10.1136/jcp.54.5.356
  • Ledwozyw, A., Michalak, J., Stepien, A.K. & Adziolka, A. (1986). The relationship between plasma triglycerides, total lipids and lipid peroxidation products during huma atherosclerosis. Clinica Chimica Acta, 155: 275-284. doi: 10.1016/0009-8981(86)90247-0
  • Lu, C. & Liu, Y. (2002). Interaction of lipoic acid radical cations with vitamins C and E analogue and hydroxycinnamic acid derivatives. Archives of Biochemistry and Biophysics, 406: 78-84. doi: 10.1016/s0003-9861(02)00411-3
  • Mahfouz, R., Sharma, R., Sharma, D., Sabanegh, E. & Agarwal, A. (2009). Diagnostic value of the total antioxidant capacity (TAC) in human seminal plasma. Fertility and Sterility, 91:805-811. doi: 10.1016/j.fertnstert.2008.01.022
  • Marklund, S. & Marklund, G. (1974). Involvement of the superoxyde anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxyde dismutase. European Journal of Biochemistry, 47:469-474. doi: 10.1111/j.1432-1033.1974.tb03714.x
  • McCord, J.M. & Fridovich, I. (1969). Superoxide dismutase: an enzymic function for erythrocuprein. Journal of Biological Chemistry, 244: 6049-6055. doi: 10.1074/jbc.244.22.6049
  • Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V. & Milner, A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84:407-412. doi: 10.1042/cs0840407
  • Monterio, D.A., Almeida, J.A.D., Rantin, F.T. & Kalinin, A.L. (2006). Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology, 141:143-149. doi: 10/1016 j.cbpc.2006.01.004
  • Monterio, D.A., Rantin, F.T. & Kalinin, A.L. (2009). The effects of selenium on oxidative stress biomarkers in the freshwater characid fish matrinxa, brycon cephalus exposed to organophosphate insecticide folisuper 600 BR (methyl parathion). Comparative Biochemistry and Physiology, 149:40-49. doi: 10.1016/j.cbpc.2008.6.012
  • Moreau, R., Dabrowski, K., Czesny, S., Chila, F. (1999). Vitamin C-vitamin E interaction in juvenile lake sturgeon (Acipenser fulvescens), a fish able synthesize ascorbic acid. Journal of Applied Ichthyology, 15: 205-257. doi: 10.1111/j.1439-0426-1999/tb00245.x
  • Naqvi, S.M, Vaishnavi, C. (1993). Bioaccumulative potential and toxicity of endosulfan insecticide to non-target animals. Comparative Biochemistry and Physiology, 105:347-361. doi: 10/1016/0300-9629(93)90219-T
  • Oruc, E.O. (2010). Oxidative stress, steroid hormone concentrations and acetylcholinesterase activity in Oreochromis niloticus exposed to chlorpyrifos. Pesticide Biochemistry and Physiology, 96: 160-166. doi: 10.1016/j.pestbp.2009.11.005
  • Prior, R.L. & Cao, G. (1999). In vivo total antioxidant capacity: comparison of different analytical methods. Free Radical Biology and Medicine, 27:1173-1181. doi: 10.1016/s0891(99)00203-8
  • Schlenk, D. (2005). Pesticide biotransformation in fish. In: Mommsen TP, Moon TW (Eds) Biochemistry and molecular biology of fishes, 6th edn, pp 171-190. Amsterdam: Elsevier.
  • Singh, M., Sandhir, R. & Kiran, R. (2011). Effects on antioxidant status of liver following atrazine exposure and its attenuation by vitamin E. Experimental and Toxicologic Pathology, 63: 269-276. doi: 10.1016/j.ept.2010.01.005
  • Turkez, H. & Togar, B. (2011). Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes. The Journal of Toxicological Sciences, 36: 531-537. doi: 10.2131/jts.36.531
  • Tsao, C.S. (1997). An overview of ascorbic acid chemistry and biochemistry In: Packer L, Fuchs J (eds) Vitamin C in health and disease, pp 25-58. New York: Marcel Dekker.
  • Ural, M.S. (2013). Chlorpyrifos-induced changes in oxidant/antioxidant status and haematological parameters of Cyprinus carpio: Ameliorative effect of lycopene. Chemosphere, 90:2059-2064. doi:10.1016/j. Chemosphere.2012.12.006
  • Uner, N., Oruc, E.O., Sevgiler, Y., Sahin, N., Durmaz, H. & Usta, D. (2006). Effects of diazinon on acetylcholinesterase activity and lipid peroxidation in the brain of Oreochromis niloticus. Environmental Toxicology and Pharmacology, 21:241-245. doi: 10.1016/j.etap.2005.08.007
  • Valavanidis, A., Vlahogianni, T., Dassenakis, M. & Scoullos, M. (2006). Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicology and Environmental Safety, 64:178-189. doi: 10.1016/j.ecoenv/2005.03.013
  • Vale, J.A. (1998). Toxicokinetic and toxicodynamic aspects of organophosphorus OP insecticide poisoning. Toxicology Letters, 64:102-103. doi: 10.1016/s0378-4274(98)80136-7
  • Verlhac, V. & Gabaudan, J. (1997). The effect of vitamin C on Fish Health. Roche Technical Buletin, Hoffmann-La Roche Ltd, Basel, Switzerland, 30 pp.
  • Verlhac, V., Obach, A., Gabaudan, J., Schüep, W. & Hole, R. (1998). Immunomodulation by dietary vitamin C and glucan in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 8:409-424. doi: 10.1006/fsim/1998.0148
  • Virtue, W.A. & Clayton, J.W. (1997). Sheep dip chemicals and water pollution. Science of the Total Environment, 194-195:207-217. doi: 10.1016/s0048-9697(96)05365-x
  • Winston, G.W, Regoli, F., Dugas, A.J, Fong, J.H. & Blanchard, K.A. (1998). A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids. Free Radical Biology and Medicine, 24:480-493. doi: 10.1016/s0891-5849(97)00277-3
  • Xing, H., Li, S., Wong, Z., Gao, X., Xu, S. & Wang, X.M. (2012). Oxidative stress response and histopathological changes due to atrazine and chlorpyrifos exposure in common carp. Pesticide Biochemistry & Physiology,103: 74-80. doi: 10.1016/j.pestbp.2013.03.007
  • Zama, D., Meraihi, Z., Tebibel, S., Benayssa, W., Benayach, F., Benayache, S. & Vlitinck, A.J. (2007). Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: the protective role of the butanolic extract of Paronychie argentea L. Indian Journal of Pharmacology, 39:145-150. doi: 10.4103/0253-7613.33434
Journal Section Articles

Author: Alireza MİRVAGHEFİ

Author: Mohsen ALİ



Application Date : May 15, 2016
Acceptance Date : July 6, 2020
Publication Date : May 15, 2016

APA Mi̇rvaghefi̇, A , Ali̇, M , Poorbagher, H . (2016). Effects of vitamin C on oxidative stress parameters in rainbow trout exposed to diazinon. Ege Journal of Fisheries and Aquatic Sciences , 33 (2) , 113-120 . DOI: 10.12714/egejfas.2016.33.2.04