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Ulva sp.'nin aktif bileşiklerinin elde edilmesi için ekstraksiyon koşullarının optimizasyonu

Year 2024, Volume: 41 Issue: 3, 194 - 206, 15.09.2024

Gizem Öder , Oguz Bayraktar , Beyza Tutku Bıçakçı

https://doi.org/10.12714/egejfas.41.3.04

Abstract

Deniz marulu olarak bilinen yeşil bir makroalg olan Ulva sp., antimutajenik, antikoagülan, antikanser, antiinflamatuar, antibakteriyel ve nutrasötik özelliklere sahip polisakkaritler, proteinler, mineraller ve biyoaktif bileşikler açısından zengindir. Ege Denizi kıyılarında bol miktarda bulunması, genellikle atık olarak atıldığı için çevresel bir zorluk oluşturmaktadır. Ancak Ulva sp. kozmetiklerde ve diyet takviyelerinde yüksek değerli ürünler için potansiyel taşımaktadır. Tepki yüzey metodolojisi kullanılarak biyoaktif bileşiklerinin ekstraksiyonunun optimize edilmesi, etanol konsantrasyonunun, katı/sıvı oranının ve ekstraksiyon süresinin ayarlanmasını içermektedir. Değerlendirilen temel tepkiler arasında verim, toplam polisakkaritler, toplam protein, toplam fenol, toplam antioksidan aktivite, alfa-glukozidaz inhibitör aktivitesi ve maya hücresi glikoz alımı yer almıştır. Bu çalışmada, ekstraksiyon koşullarındaki değişikliklere bağlı olarak ekstraksiyon verimleri %0,86 ile %22,47 arasında değişmiştir. En yüksek toplam protein içeriği 106.88 mg BSA/g kuru ekstre iken, polisakkarit içeriği %15.42 olarak belirlendi. Toplam fenol içeriği ve antioksidan kapasitesi için en yüksek değerler sırasıyla 82.15 mg GAE/g kuru ekstre ve 63.63 mg Trolox/g kuru ekstre olarak bulundu. Ekstraktlardaki toplam antioksidan ve fenolik bileşik miktarlarının belirlenmesi, potansiyel uygulamalarını genişletebilir. Ayrıca, Ulva sp. ekstraktlarının diyabet tedavisinde inhibitör olarak potansiyel uygulaması, hem alfa-glukozidaz enzim inhibisyonunu hem de maya hücrelerinde glikoz alımını değerlendiren deneyler yoluyla gösterilmiştir. Sonuçlar, Ulva sp.'nin atıktan değerli antidiyabetik ürünlere dönüştürülmesi için çevre dostu bir yaklaşımı desteklemektedir.

Keywords

Ulva sp. tepki yüzey metodolojisi optimizasyon biyoaktivite karakterizasyon

Project Number

223M357

References

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Optimization of extraction conditions for obtaining active compounds of Ulva sp.

Year 2024, Volume: 41 Issue: 3, 194 - 206, 15.09.2024

Gizem Öder , Oguz Bayraktar , Beyza Tutku Bıçakçı

https://doi.org/10.12714/egejfas.41.3.04

Abstract

Ulva sp., a green macroalgae known as sea lettuce, is rich in polysaccharides, proteins, minerals, and bioactive compounds with antimutagenic, anticoagulant, anticancer, anti-inflammatory, antibacterial, and nutraceutical properties. Its abundance along the Aegean Sea coast poses an environmental challenge, as it is often disposed of as waste. However, Ulva sp. holds potential for high-value products in cosmetics and dietary supplements. Optimizing the extraction of its bioactive compounds using response surface methodology involved adjusting ethanol concentration, solid/liquid ratio, and extraction time. Key responses evaluated included yield, total polysaccharides, total protein, total phenol, total antioxidant activity, alpha-glucosidase inhibitory activity, and yeast cell glucose uptake. In this study, extraction yields ranged from 0.86% to 22.47% based on variations in extraction conditions. The highest total protein content was 106.88 mg BSA/g dry extract, while the polysaccharide content was determined to be 15.42%. The highest values for total phenol content and antioxidant capacity were found to be 82.15 mg GAE/g dry extract and 63.63 mg Trolox/g dry extract, respectively. The determination of the total amounts of antioxidants and phenolic compounds in extracts may expand their potential applications. In addition, the potential application of Ulva sp extracts as inhibitors for the treatment of diabetes has been demonstrated through experiments assessing both alpha-glucosidase enzyme inhibition and glucose uptake in yeast cells. The results support an environmentally friendly approach for the utilization of Ulva sp. from waste into valuable antidiabetic products.

Keywords

Ulva sp. response surface methodology optimization bioactivity characterization

Ethical Statement

Ethical approval is not required for this study.

Supporting Institution

Turkish Scientific and Technological Research Institution (Grant number: 223M357)

Project Number

223M357

References

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  • Abd-Ellatef, G.E.F., Ahmed, O.M., Abdel-Reheim, E.S., & Abdel-Hamid, A.H.Z. (2017). Ulva lactuca polysaccharides prevent wistar rat breast carcinogenesis through the augmentation of apoptosis, enhancement of antioxidant defense system, and suppression of inflammation. Breast Cancer: Targets and Therapy, 9, 67 83. https://doi.org/10.2147/BCTT.S125165
  • Abou El Azm, N., Fleita, D., Rifaat, D., Mpingirika, E.Z., Amleh, A., & El-Sayed, M.M. (2019). Production of bioactive compounds from the sulfated polysaccharides extracts of Ulva lactuca: Post-extraction enzymatic hydrolysis followed by ion-exchange chromatographic fractionation. Molecules, 24(11), 2132. https://doi.org/10.3390/molecules24112132
  • Alghazeer, R., Whida, F., Abduelrhman, E., Gammoudi, F., & Azwai, S. (2013). Screening of antibacterial activity in marine green, red and brown macroalgae from the western coast of Libya. Natural Science, 5(1), 7-14. https://doi.org/10.4236/ns.2013.51002
  • Alsufyani, T., Califano, G., Deicke, M., Grueneberg, J., Weiss, A., Engelen, A. H., ... & Wichard, T. (2020). Macroalgal-bacterial interactions: Identification and role of thallusin in morphogenesis of the seaweed Ulva (Chlorophyta). Journal of Experimental Botany, 71(11), 3340-3349. https://doi.org/10.1093/jxb/eraa066
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  • Ben Amor, C., Jmel, M.A., Chevallier, P., Mantovani, D., & Smaali, I. (2023). Efficient extraction of a high molecular weight ulvan from stranded Ulva sp. biomass: Application on the active biomembrane synthesis. Biomass Conversion and Biorefinery, 1-11.
  • Chen, J., Zeng, W., Gan, J., Li, Y., Pan, Y., Li, J., & Chen, H. (2021). Physicochemical properties and anti-oxidation activities of ulvan from Ulva ertusa Kjellm. Algal Research, 55, 102269. https://doi.org/10.1016/j.algal.2021.102269
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There are 55 citations in total.

Details

Primary Language English
Subjects Chemical Engineering (Other)
Journal Section Articles
Authors

Gizem Öder 0000-0002-5386-2822

Oguz Bayraktar 0000-0003-4210-2825

Beyza Tutku Bıçakçı 0000-0002-6638-0355

Project Number 223M357
Early Pub Date September 2, 2024
Publication Date September 15, 2024
Submission Date April 5, 2024
Acceptance Date July 29, 2024
Published in Issue Year 2024Volume: 41 Issue: 3

Cite

APA Öder, G., Bayraktar, O., & Bıçakçı, B. T. (2024). Optimization of extraction conditions for obtaining active compounds of Ulva sp. Ege Journal of Fisheries and Aquatic Sciences, 41(3), 194-206. https://doi.org/10.12714/egejfas.41.3.04