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Karyotypic analysis of Chondrostoma regium (Teleostei: Leuciscidae) distributed in the Karasu River (Erzurum)

Year 2023, Volume: 40 Issue: 1, 62 - 68, 15.03.2023
https://doi.org/10.12714/egejfas.40.1.09

Abstract

In this study, the karyotypic characteristics of Chondrostoma regium (Heckel, 1843) have been investigated. Fish samples were caught from the Karasu River (Euphrates River Basin) with fishing net. The live fish were transported to the laboratory and kept in aerated aquaria before the analyses. The karyotype analysis was performed in fish kidney and gill epithelium cells. It was determined that C. regium had 2n=50 chromosomes. In detail, the karyotype formula of C. regium was determined as 9 metacentric, 7 submetacentric, 1 subtelocentric and 8 telocentric chromosome pairs (18M+14SM+2ST+16T), and fundamental arm number was calculated as 82. Constitutive heterochromatin regions were determined on telomeres of the chromosomes. Nuclear orgnizer regions were detected on 21st chromosome. Karyotype symmetry/asymmetry index was calculated as 2.32. The karyotypes of gill and kidney cells were the same. No sex chromosomes were cytologically detected.

Supporting Institution

Atatürk University Research Fund

Thanks

This research was supported by Atatürk University Research Fund (BAP: 2012/477). This study is prepared from Büşra Yamaç’s master thesis.

References

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Karasu ırmağında (Erzurum) yayılış gösteren Chondrostoma regium'un (Teleostei: Leuciscidae) karyotipik analizleri

Year 2023, Volume: 40 Issue: 1, 62 - 68, 15.03.2023
https://doi.org/10.12714/egejfas.40.1.09

Abstract

Bu çalışmada Chondrostoma regium'un (Heckel, 1843) karyotipik özellikleri araştırılmıştır. Balık numuneleri Karasu Irmağından (Fırat Nehri havzası) serpme ağlarla yakalanmıştır. Analizlerden önce canlı balıklar laboratuvara taşınmış ve havalandırmalı akvaryumlarda tutulmuştur. Karyotip analizi balıkların böbrek ve solungaç epitel hücrelerinde yapılmıştır. C. regium'un 2n=50 kromozoma sahip olduğu belirlenmiştir. Ayrıntılı olarak C. regium'un karyotip formülü 9 metasentrik, 7 submetasentrik, 1 subtelosentrik ve 8 telosentrik kromozom çifti (18M+14SM+2ST+16T) olarak belirlenmiş olup, temel kol sayısı 82 olarak hesaplanmıştır Kromozomların telomerlerinde konstitütif heterokromatin bölgeleri belirlenmiştir. 21. kromozom üzerinde nüklear organizer bölge tespit edilmiştir. Karyotip simetri/asimetri indeksi 2,32 olarak bulunmuştur. Solungaç ve böbrek hücrelerinin karyotipi aynı olup sitolojik olarak hiçbir eşey kromozomu tespit edilmemiştir.

References

  • Al-Marjan, K.S.N. (2016). Seasonal variations and prevalence of infections of some species of ectoparasites affecting freshwater fish, Chondrostoma regium from Greater Zab river, Kurdistan region, Iraq. PolyTechnic, 6(1), 310-315.
  • Amemiya, C.T., & Gold, J.R. (1988). Chromosomal NORs as taxonomic and systematic characters in North American cyprinid fishes. Genetica, 76(2), 81-90. https://doi.org/10.1007/BF00058806 Arai, R. (2011). Fish Karyotypes: A check list. Springer e–book. Tokyo Berlin Heidelberg New York. https://doi.org/10.1007/978-4-431-53877-6
  • Araya-Jaime, C., Palma-Rojas, C., Von Brand, E., & Silva, A. (2020). Cytogenetic characterization, rDNA mapping and quantification of the nuclear DNA content in Seriolella violacea Guichenot, 1848 (Perciformes, Centrolophidae). Comparative Cytogenetics, 14(3), 319. https://doi.org/10.3897/CompCytogen.v14i3.53087
  • Arslan, A., & Gündoğdu, H. (2016). Cytogenetic studies on the endemic Beyşehir nase, Chondrostoma beysehirense (Bogutskaya, 1997) in Turkey. Caryologia, 69(2), 116-120. https://doi.org/10.1080/00087114.2015.1109958
  • Barshiene, J.V. (1977). Karyotype studies in the under mouth Chondrostoma nasus. Citologia, 19, 390–392. (in Russian)
  • Berberović, L.J., Hadžiselimović, R. & Sofradžija, A. (1970). Comparative review on the basic data on the chromosome complements of Chondrostoma phoxinus Heckel and Chondrostoma knerii Heckel. Ichthyologia, 2(1), 25–30. (in Serbo-Croatian)
  • Bianco, P.G., Aprea, G., Balletto, E., Capriglione, T., Fulgione, D., & Odierna, G. (2004). The karyology of the cyprinid genera Scardinius and Rutilus in southern Europe. Ichthyological Research, 51(3), 274-278. https://doi.org/10.1007/s10228-004-0247-1
  • Bilecenoğlu, M., Kaya, M., Cihangir, B., & Çiçek, E. (2014). An updated checklist of the marine fishes of Turkey. Turkish Journal of Zoology, 38(6), 901-929. https://doi.org/10.3906/zoo-1405-60
  • Boroń, A., Grabowska, A., Spóz, A., & Przybył, A. (2020). B chromosomes and cytogenetic characteristics of the common nase Chondrostoma nasus (Linnaeus, 1758). Genes, 11(11), 1317. https://doi.org/10.3390/genes11111317
  • Cataudella, S., Sola, L., Accame Muratori, R., & Capanna, E. (1977). The chromosomes of 11 species of Cyprinidae and one Cobitidae from Italy, with some remarks on the problem of polyploidy in the Cypriniformes. Genetica, 47(3), 161-171. https://doi.org/10.1007/BF00123236
  • Collares-Pereira, M.J. (1992). In vivo direct chromosome preparation (protocol for air drying technique). Paper presented at the First International Workshop on Fish Cytogenetic Techniques. 14–24 September, Concarneau, France.
  • Collares-Pereira, M.J., & Ráb, P. (1999). NOR polymorphism in the Iberian species Chondrostoma lusitanicum (Pisces: Cyprinidae) re-examination by FISH. Genetica, 105(3), 301-303. https://doi.org/10.1023/A:1003885922023
  • Cucchi, C., & Baruffaldi, A. (1990). A new method for karyological studies in teleost fishes. Journal of Fish Biology, 37(1), 71-75. https://doi.org/10.1111/j.1095-8649.1990.tb05928.x
  • Çiçek, E., Birecikligil, S.S., & Fricke, R. (2015). Freshwater fishes of Turkey: a revised and updated annotated checklist. Biharean Biologist, 9 (2), 141-157.
  • Çiftçi, Y., Gül Mutlu, A., Güçlü, S.S., Turan, D., & Küçük, F. (2020). Phylogeography of the genus Chondrostoma Agassiz, 1835 (Teleostei: Leuciscidae) in Anatolia, as inferred from mitochondrial DNA analysis. Zoology in the Middle East, 66(3), 206-221. https://doi.org/10.1080/09397140.2020.1788255
  • Dai, Y., & Han, H. (2018). Karyological analysis of two species in the subfamily Schizothoracinae (Cypriniformes: Cyprinidae) from China, with notes on karyotype evolution in Schizothoracinae. Turkish Journal of Fisheries and Aquatic Sciences, 18(1), 175-186. https://doi.org/10.4194/1303-2712-v18_1_20
  • Denton, T.E. (1973). Fish chromosome methodology. Charles C. Thomas Publisher, Springfifield, USA.
  • Eagderi, S., Jouladeh-Roudbar, A., Birecikligil, S. S., Çiçek, E., & Coad, B.W. (2017). Chondrostoma esmaeilii, a new cyprinid species from the Tigris river drainage in Iran (Teleostei: Cyprinidae). Vertebrate Zoology, 67(2), 125-132.
  • Eroğlu, H.E. (2015). Which chromosomes are subtelocentric or acrocentric? A new karyotype symmetry/asymmetry index. Caryologia, 68(3), 239-245. https://doi.org/10.1080/00087114.2015.1032614
  • Esmaeili, H.R., Zareian, H., Gholamhosseini, A., Ebrahimi, M., Gholami, Z., Teimori, A., & Ansari, T. H. (2010). Karyotype analysis of the king nase fish, Chondrostoma regium (Heckel, 1843) (Actinopterygii: Cyprinidae) from Iran. Turkish Journal of Fisheries and Aquatic Sciences, 10(4). https://doi.org/10.4194/trjfas.2010.0406
  • Freyhof, J., & Özuluğ, M. (2009). Pseudophoxinus evliyae, a new species of spring minnow from Western Anatolia with remarks on the distribution of P. ninae and the systematic position of P. fahirae (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters, 20, 309-318.
  • Freyhof, J. (2014). Chondrostoma beysehirense. The IUCN Red List of Threatened Species 2014: e.T61366A19010470. https://dx.doi.org/10.2305/IUCN.UK.2014-1.RLTS.T61366A19010470.en. (Accessed on 19 January 2022).
  • Gaffaroğlu, M., & Yüksel, E. (2009). Constitutive heterochromatin in Acanthobrama marmid and Cyprinion macrostomus (Osteichthyes, Cyprinidae). Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15, 169-172. https://doi.org/10.9775/kvfd.2008.72-A
  • Geng, L.W., Xu, W., Jiang, H.F., & Tong, G.X. (2013). Karyotype analysis of Barbus capito (Güldentstädt, 1773) using curve measurement software. Journal of Applied Ichthyology, 29(4), 922-924. https://doi.org/10.1111/jai.12142
  • Goes, C.A.G., Daniel, S.N., Piva, L. H., Yasui, G.S., Artoni, R.F., Hashimoto, D.T., & Porto-Foresti, F. (2020). Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907. Comparative Cytogenetics, 14(2), 231–242. https://doi.org/10.3897/CompCytogen.v14i2.49513
  • Gold, J.R., Amemiya, C.T., & Ellison, J.R. (1986). Chromosomal heterochromatin differentiation in North American cyprinid fishes. Caryologia, 51, 557-566. https://doi.org/10.1508/cytologia.51.557
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Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Büşra YAMAÇ 0000-0002-0469-3321

Turgay ŞİŞMAN 0000-0002-1098-1741

Project Number BAP: 2012/477
Publication Date March 15, 2023
Submission Date October 21, 2022
Published in Issue Year 2023Volume: 40 Issue: 1

Cite

APA YAMAÇ, B., & ŞİŞMAN, T. (2023). Karyotypic analysis of Chondrostoma regium (Teleostei: Leuciscidae) distributed in the Karasu River (Erzurum). Ege Journal of Fisheries and Aquatic Sciences, 40(1), 62-68. https://doi.org/10.12714/egejfas.40.1.09