Research Article
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Integrated assessment with biomarker responses and metal concentrations on some fish species from İzmir Bay: A preliminary investigation

Year 2022, Volume: 39 Issue: 4, 284 - 292, 15.12.2022

Mustafa Bilgin , Esin Uluturhan Suzer , Enis Darılmaz

https://doi.org/10.12714/egejfas.39.4.03

Abstract


Antioxidant related biomarkers (superoxide dismutase, catalase, malondialdehyde) were investigated to evaluate metal (Hg, Cd, Pb, Cr, Cu, Zn, and Mn) bioaccumulation in some organs of fish species (Sparus aurata, Chelon labrosus, Diplodus vulgaris) from the İzmir Bay. Samples were collected at November 2019 from Inner and Outer Bays. Metal and biomarker analyses were carried out by Atomic Absorption Spectrometer and microplate reader, respectively. For metal analyses in organs, higher metal bioaccumulations were found at liver tissues. Higher Hg and Zn concentrations were found in S. aurata, higher Cd, Cr, Cu and, Mn concentrations were detected in C. labrosus and higher Pb concentrations were determined in D. vulgaris. In biomarker results, superoxide dismutase (SOD) and catalase (CAT) activities were generally higher in gills, however, MDA contents were higher at liver. The highest biomarker results were detected at C. labrosus. Statistical analyses were demonstrated that especially MDA content were expressed strong responses for the metal bioaccumulations. Also, Mn levels were considerably correlated with all biomarkers as expected. This study revealed that combined utilization of biomarkers and metal concentrations could be a vital indicator to investigate health status of the marine ecosystems.

Keywords

Catalase superoxide dismutase malondialdehyde trace metals mullet Sparidae sp.

Supporting Institution

Dokuz Eylül University

Project Number

2019.KB.FEN.040

Thanks

This study is represented a part of the PhD thesis of Mustafa BİLGİN. The study was supported by Dokuz Eylül University, Department of Scientific Research Projects (Project Number: 2019.KB.FEN.040). Authors would like to thank to their colleagues for the contributions at the laboratory and the field

References

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İzmir Körfezi’ndeki bazı balık türlerinde biyoişaretçi yanıtlarının metal konsantrasyonları ile birlikte değerlendirilmesi: Bir ön araştırma

Year 2022, Volume: 39 Issue: 4, 284 - 292, 15.12.2022

Mustafa Bilgin , Esin Uluturhan Suzer , Enis Darılmaz

https://doi.org/10.12714/egejfas.39.4.03

Abstract


İzmir Körfezi’ndeki balık türlerinin (Sparus aurata, Chelon labrosus, Diplodus vulgaris) organlarında metal (Hg, Cd, Pb, Cr, Cu, Zn ve Mn) biyobirikimini değerlendirmek için antioksidanla kökenli biyoişaretçiler (süperoksit dismutaz, katalaz, malondialdehit) incelenmiştir. Balıklar Kasım 2019'da İç ve Dış Körfez’den toplanmıştır. Metal ve biyoişaretçi analizleri sırasıyla Atomik Absorpsiyon Spektrometresi ve mikroplaka okuyucu ile yapılmıştır. Organlarda bulunan metal değerlerine göre karaciğer dokularında daha yüksek metal biyobirikimleri bulunmuştur. S. aurata'da daha yüksek Hg ve Zn konsantrasyonları, C. labrosus'ta daha yüksek Cd, Cr, Cu ve Mn konsantrasyonları ve D. vulgaris'te ise daha yüksek Pb konsantrasyonları tespit edilmiştir. Biyoişaretçi sonuçlarına göre, solungaçlarda SOD ve CAT aktiviteleri genel olarak daha yüksek, ancak MDA içerikleri karaciğerde daha yüksek bulunmuştur. En yüksek biyoişaretçi bulguları ise C. labrosus'ta tespit edilmiştir. İstatistiksel hesaplamalar, özellikle MDA içeriğinin, metal biyobirikimleri için kayda değer cevaplar verdiğini göstermiştir. Ayrıca Mn seviyeleri, beklendiği gibi tüm biyoişaretçiler ile anlamlı korelasyonlar göstermiştir. Bu çalışma, biyoişaretçilerin ve metal konsantrasyonlarının birlikte kullanımının deniz ekosistemlerinin sağlık durumunu araştırmak için önemli bir belirteç olabileceğini ortaya koymuştur.


Keywords

Katalaz süperoksit dismutaz malondialdehit iz metaller kefal Sparidae sp.

Project Number

2019.KB.FEN.040

References

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  • Bauchot, M.L., & Hureau, J.C. (1990). Sparidae. 790-812pp. Check-List of the Fishes of the Eastern Tropical Atlantic (CLOFETA), 2.
  • Beg, M.U., Al-Jandal, N., Al-Subiai, S., Karam, Q., Husain, S., Butt, S.A., Ali, A., Al-Hasan, E., Al-Dufaileej, S., & Al-Husaini, M. (2015). Metallothionein, oxidative stress and trace metals in gills and liver of demersal and pelagic fish species from Kuwaits’ marine area. Marine Pollution Bulletin, 100(2), 662–672. DOI: 10.1016/j.marpolbul.2015.07.058
  • Bilbao, E., Raingeard, D., de Cerio, O. D., Ortiz-Zarragoitia, M., Ruiz, P., Izagirre, U., Orbea, A., Marigómez, I., Cajaraville, M. P., & Cancio, I. (2010). Effects of exposure to Prestige-like heavy fuel oil and to perfluorooctane sulfonate on conventional biomarkers and target gene transcription in the thicklip grey mullet Chelon labrosus. Aquatic Toxicology, 98(3), 282–296. DOI: 10.1016/j.aquatox.2010.02.018
  • Briffa, J., Sinagra, E., & Blundell, R. (2020). Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon, 6(9), e04691.
  • Cajaraville, M. P., Bebianno, M. J., Blasco, J., Porte, C., Sarasquete, C., & Viarengo, A. (2000). The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian Peninsula: A practical approach. Science of the Total Environment, 247(2–3), 295–311. DOI: 10.1016/S0048-9697(99)00499-4
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  • Cengiz, Ö., Paruğ, Ş.Ş., & Kızılkaya, B. (2019). Maximum length record of common two-banded seabream (Diplodus vulgaris Geoffroy Saint-Hilaire, 1817) for Aegean Sea with Turkish Waters. Alınteri Zirai Bilimler Dergisi, 34, 160–163. DOI: 10.28955/alinterizbd.638974
  • Chacón Aranda, M.F., Fanarraga Tasayco, G.B., Castañeda Olivera, C.A., Benites Alfaro, E.G., & Acosta Suasnabar, E.H. (2021). Microplastics in water and their influence in the species Chelon labrosus. Proceedings of the LACCEI International Multi-Conference for Engineering, Education and Technology, 2021-July, 19–24. DOI: 10.18687/LACCEI2021.1.1.233
  • Cretì, P., Trinchella, F., & Scudiero, R. (2010). Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems. Environmental Monitoring and Assessment, 165(1–4), 321–329. DOI: 10.1007/s10661-009-0948-z
  • Da Palma, J. A. M. (2002). Morphological variation in eight Sparidae species of the Eastern Atlantic and Mediterranean Sea. Academy of Management Journal. 226 p. Portugal.
  • da Silva, C.A., Garcia, C.A.B., de Santana, H.L.P., de Pontes, G.C., Wasserman, J.C., & da Costa, S.S.L. (2021). Metal and metalloid concentrations in marine fish marketed in Salvador, BA, northeastern Brazil, and associated human health risks. Regional Studies in Marine Science, 43, 101716. DOI: 10.1016/j.rsma.2021.101716
  • Di Giulio, R.T., Washburn, P.C., Wenning, R.J., Winston, G.W., & Jewell, C.S. (1989). Biochemical responses in aquatic animals: a review of determinants of oxidative stress. Environmental Toxicology and Chemistry, 8, 1103–1123.
  • Farombi, E.O., Adelowo, O.A., & Ajimoko, Y.R. (2007). Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigeria Ogun River. International Journal of Environmental Research and Public Health, 4(2), 158–165. DOI: 10.3390/ijerph2007040011
  • Gusso-Choueri, P.K., Choueri, R.B., de Araújo, G.S., Cruz, A.C.F., Stremel, T., Campos, S., Abessa, D.m.S., & Ribeiro, C.A.O. (2015). Assessing pollution in marine protected areas: the role of a multi-biomarker and multi-organ approach. Environmental Science and Pollution Research, 22(22), 18047–18065. DOI: 10.1007/s11356-015-4911-y
  • Hadj Taieb, A., Sley, A., Ghorbel, M., & Jarboui, O. (2013). Feeding habits of Sparus aurata (Sparidae) from the Gulf of Gabes (central Mediterranean). Cahiers de Biologie Marine, 54(2), 263–270.
  • He, X., Nie, X., Yang, Y., Liu, X., Pan, D., Cheng, Z., & Liang, X. (2012). Multi-biomarker responses in fishes from two typical marine aquaculture regions of South China. Marine Pollution Bulletin, 64(11), 2317–2324. DOI: 10.1016/j.marpolbul.2012.09.002
  • Islam, M.S., Ahmed, M. K., Raknuzzaman, M., Habibullah-Al-Mamun, M., & Masunaga, S. (2015). Metal Speciation in Sediment and Their Bioaccumulation in Fish Species of Three Urban Rivers in Bangladesh. Archives of Environmental Contamination and Toxicology, 68(1), 92–106. DOI: 10.1007/s00244-014-0079-6
  • Kacar, A., Kocyigit, A. & Uluturhan, E. (2016) Concentration Of Heavy Metals and Investigation of Bacterial Toxic Metal Resistance in Costal City Sediments (Eastern Aegean Sea) Fresenius Environmental Bulletin, 25(1), 55-66.
  • Kontas, A., Alyuruk, H., Bilgin, M., Uluturhan, E., Ünlüoğlu, A., Darilmaz, E., & Altay, O. (2022). Metal Bioaccumulation and Potential Health Risk Assessment in Different Tissues of Three Commercial Fish Species (Merluccius merluccius, Mullus barbatus, and Pagellus erythrinus) from Edremit Bay (Aegean Sea), Turkey. Biological Trace Element Research, 200(2), 868-880.
  • Kucuksezgin, F., Kontas, A., Altay, O., Uluturhan, E., & Darılmaz, E. (2006). Assessment of marine pollution in Izmir Bay: Nutrient, heavy metal and total hydrocarbon concentrations. Environment international, 32(1), 41-51.
  • Kucuksezgin,F., Kontas, A., & Uluturhan, E. (2011) Evaluations of heavy metal pollution in sediment and Mullus barbatus from the Izmir Bay (Eastern Aegean) during 1997–2009, Marine Pollution Bulletin, 62, (7), 1562-2011. DOI: 10.1016/j.marpolbul.2011.05.012
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There are 53 citations in total.

Details

Primary Language English
Subjects Maritime Engineering (Other)
Journal Section Articles
Authors

Mustafa Bilgin 0000-0003-4680-5931

Esin Uluturhan Suzer 0000-0002-7886-922X

Enis Darılmaz 0000-0002-9090-9319

Project Number 2019.KB.FEN.040
Publication Date December 15, 2022
Submission Date June 27, 2022
Published in Issue Year 2022Volume: 39 Issue: 4

Cite

APA Bilgin, M., Uluturhan Suzer, E., & Darılmaz, E. (2022). Integrated assessment with biomarker responses and metal concentrations on some fish species from İzmir Bay: A preliminary investigation. Ege Journal of Fisheries and Aquatic Sciences, 39(4), 284-292. https://doi.org/10.12714/egejfas.39.4.03