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Diagnosis of bacterial fish diseases and classification of serotypes with slide agglutination method

Year 2022, Volume: 39 Issue: 2, 97 - 103, 15.06.2022
https://doi.org/10.12714/egejfas.39.2.02

Abstract



Bacterial fish pathogens cause significant losses in rainbow trout farms. In fish farms, bacterial pathogens cause threatening diseases which have made necessary to develop rapid methods for disease diagnosis. Serological techniques which are applied with a small amount of antiserum and sample, are preferred for the rapid diagnosis of fish diseases. In this study, formalin-killed antigens prepared from reference strains of Lactococcus garvieae, Yersinia ruckeri, and Vibrio (Listonella) anguillarum were injected intravenously in consecutive doses to New Zealand rabbits. One week after the last injection, the sera separated to use in the slide agglutination tests. A total of 42 strains were studied, including Y. ruckeri (18 isolates), V. anguillarum (14 isolates), and L. garvieae (7 isolates) and 3 reference (ATCC 43305, ATCC 29473, ATCC 49156) strains. Serotype O1 determined predominant serotype (86%) in V. anguillarum and Y. ruckeri (84%) strains examined by slide agglutination method. L. garvieae strains did not react against Japanese antisera but positively reacted against Turkish L. garvieae antisera.


Supporting Institution

Izmir Katip Celebi University Scientific Research Projects Coordinatorship

Thanks

This study presents a part of Kaan KUMAŞ’s master thesis titled “Diagnosis of bacterial fish diseases with using slide agglutination method” (with English abstract) and was supported by İzmir Katip Çelebi University Scientific Research Projects Coordinatorship with the project number 2021-TYL-FEBE-0012.

References

  • Altun, S., Onuk, E.E., Ciftci, A., Duman, M., & Büyükekiz, A.G. (2013). Determination of phenotypic, serotypic and genetic diversity and antibiotyping of Yersinia ruckeri isolated from rainbow trout. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(2), 225-232. DOI: 10.9775/kvfd.2012.7606
  • Austin, B., & Newaj-Fyzul, A. (2017). Diagnosis and control of diseases of fish and shellfish. John Wiley and Sons, Chichester, UK. DOI:10.1002/9781119152125
  • Avsever, M.L., & Ün, C. (2015). Distribution of hemolysin genes in Turkish Vibrio anguillarum isolates. Bulletin of the European Association of Fish Pathologists, 35(3), 75-84.
  • Bachrach, G., Zlotkin, A., Hurvitz, A., Evans, D.L., & Eldar, A. (2001). Recovery of Streptococcus iniae from diseased fish previously vaccinated with a Streptococcus vaccine. Applied and Environmental Microbiology, 67(8), 3756-3758.DOI:10.1128/AEM.67.8.3756-3758.2001
  • Balta, F., & Dengiz Balta, Z. (2017). Doğu Karadeniz’de yetiştiriciliği yapılan gökkuşağı alabalıkları (Oncorhynchus mykiss)’ndan izole edilen Vibrio anguillarum suşlarının serotiplendirilmesi, genetik karakterizasyonu ve antimikrobiyal duyarlılığının belirlenmesi. Ankara Universitesi Veteriner Fakultesi Dergisi, 64, 321-328. DOI:10.1501/Vetfak_0000002816
  • Balta, F., Sandalli, C., Kayis, S., & Ozgumus, O.B. (2010). Molecular analysis of antimicrobial resistance in Yersinia ruckeri strains isolated from rainbow trout (Oncorhynchus mykiss) grown in commercial fish farms in Turkey. Bulletin of the European Association of Fish Pathologists, 30(6), 211-219.
  • Balta, F., Dengiz Balta, Z., Özgümüş, O.B., & Çağırgan, H. (2016). Doğu Karadeniz Bölgesi’ndeki gökkuşağı alabalığı (Oncorhynchus mykiss) çiftliklerinde Yersinia ruckeri’nin portörlük yönünden tetkiki ve antimikrobiyal direncin tespiti. Journal of Anatolian Environmental & Animal Sciences, 1(3), 72-76. DOI:10.35229/jaes.280741
  • Barnes, A.C., & Ellis, A.E. (2004). Role of capsule in serotypic differences and complement fixation by Lactococcus garvieae. Fish and Shellfish Immunology, 16(2), 207-214. DOI:10.1016/s1050-4648(03)00079-2
  • Barnes, A.C., Guyot, C., Hansen, B. G., Mackenzie, K., Horne, M.T., & Ellis, A.E. (2002). Resistance to serum killing may contribute to differences in the abilities of capsulate and non-capsulated isolates of Lactococcus garvieae to cause disease in rainbow trout (Oncorhynchus mykiss L.). Fish and Shellfish Immunology, 12(2), 155-168. DOI:10.1006/fsim.2001.0361
  • Bastardo, A., Bohle, H., Ravelo, C., Toranzo, A.E., & Romalde, J.L. (2011). Serological and molecular heterogeneity among Yersinia ruckeri strains isolated from farmed Atlantic salmon Salmo salar in Chile. Diseases of Aquatic Organisms, 93(3), 207-214. DOI:10.3354/dao02296
  • Çağırgan, H. (2004). Biotyping of Lactococcus garvieae isolated from Turkey. Su Ürünleri Dergisi, 21(3), 267-269.
  • Davies, R.L. (1990). O-serotyping of Yersinia ruckeri with special emphasis on European isolates. Veterinary Microbiology, 22(4), 299-307. DOI:10.1016/0378-1135(90)90016-O
  • Eldar, A., Ghittino, C., Asanta, L., Bozzetta, E., Goria, M., Prearo, M., & Bercovier, H. (1996). Enterococcus seriolicida is a junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningoencephalitis in fish. Current Microbiology, 32(2), 85-88. DOI:10.1007/s002849900015
  • Eurell, T.E., Lewis, D. H., & Grumbles, L.C. (1979). Stained Bacterial Antigens for Use in Microagglutination Procedures. The Progressive Fish-Culturist, 41(2), 55-57.DOI:10.1577/1548-8659(1979)41[55:sbafui]2.0.co;2
  • Grisez, L., & Ollevier, F. (1995). Comparative serology of the marine fish pathogen Vibrio anguillarum. Applied and Environmental Microbiology, 61(12), 4367-4373. DOI:10.1128/aem.61.12.4367-4373.1995
  • Hansen, M.J., Kudirkiene, E., & Dalsgaard, I. (2020). Analysis of 44 Vibrio anguillarum genomes reveals high genetic diversity. PeerJ, 8, e10451. DOI:10.7717/peerj.10451
  • Hickey, M. E., & Lee, J.L. (2017). A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention. Reviews in Aquaculture, 10(3), 585-610. DOI:10.1111/raq.12188
  • Kang, S.H., Shin, G.W., Shin, Y.S., Palaksha, K.J., Kim, Y.R., Yang, H.H., Lee, E.R., Lee, E.G., Huh, N.E., Ju, O.M., & Jung, T.S. (2004). Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish (Sebastes schlegeli). Journal of Veterinary Science, 5(4), 387-390. DOI:10.4142/jvs.2004.5.4.387
  • Kitao, T. (1982). The methods for detection of Streptococcus sp., causative bacteria of streptococcal disease of cultured yellowtail (Seriola quinqueradiata) especially, their cultural, biochemical and serological properties. Fish Pathology, 17(1), 17-26. DOI:10.3147/jsfp.17.17
  • Kitao, T., Aoki, T., Fukudome, M., Kawano, K., Wada, Y., & Mizuno, Y. (1983). Serotyping of Vibrio anguillarum isolated from diseased freshwater fish in Japan. Journal of Fish Diseases, 6(2), 175-181. DOI:10.1111/j.1365-2761.1983.tb00064.x
  • Kitao, T., Aoki, T., & Muroga, K., (1984). Three new O-serotypes of Vibrio anguillarum [isolated from ayu, Plecoglossus altivelis]. Bulletin of the Japanese Society of Scientific Fisheries (Japan), 50(11), 1955.
  • Larsen, J.L. (1990). Vibrio anguillarum: characterization, ecology and pathogenicity. Doctoral dissertation in veterinary scicences, The Royal Veterinary and Agricultural University.
  • Larsen, J.L., & Olsen, J.E. (1991). Occurrence of plasmids in Danish isolates of Vibrio anguillarum serovars O1 and O2 and association of plasmids with phenotypic characteristics. Applied and Environmental Microbiology, 57(8), 2158-2163. DOI:10.1128/aem.57.8.2158-2163.1991
  • Leblanc, D., Mittal, K.R., Olivier, G., & Lallier, R. (1981). Serogrouping of motile Aeromonas species isolated from healthy and moribund fish. Applied and Environmental Microbiology, 42(1), 56-60. DOI:10.1128/aem.42.1.56-60.1981
  • Morita, H., Toh, H., Oshima, K., Yoshizaki, M., Kawanishi, M., Nakaya, K., Suzuki, T., Miyauchi, E., Ishii, Y., Tanabe, S., Murakami, M., & Hattori, M. (2011). Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae. PLoS One, 6(8), e23184. DOI:10.1371/journal.pone.0023184
  • Nakai, T., Muroga, K., & Wakabayashi, H. (1981). Serological properties of Pseudomonas anguilliseptica in agglutination. Bulletin of the Japanese Society of Scientific Fisheries, 47(6), 699-703. DOI:10.2331/suisan.47.699
  • Nomura, J., & Aoki, T. (1985). Morphological analysis of lipopolysaccharide from gram-negative fish pathogenic bacteria. Fish Pathology, 20(2-3), 193-197. DOI:10.3147/jsfp.20.193
  • Oinaka, D., Yoshimura, N., Fukuda, Y., Yamashita, A., Urasaki, S., Wada, Y., & Yoshida, T. (2015). Isolation of Lactococcus garvieae showing no agglutination with anti-KG-phenotype rabbit serum. Fish Pathology, 50(2), 37-43. DOI:10.3147/jsfp.50.37
  • Ooyama, T., Hirokawa, Y., Minami, T., Yasuda, H., Nakai, T., Endo, M., Ruangpan, L., & Yoshida, T. (2002). Cell-surface properties of Lactococcus garvieae strains and their immunogenicity in the yellowtail Seriola quinqueradiata. Diseases of Aquatic Organisms, 51(3): 169-177. DOI:10.3354/dao051169
  • Ormsby, M.J., & Davies, R.L. (2021). Diversification of OmpA and OmpF of Yersinia ruckeri is independent of the underlying species phylogeny and evidence of virulence-related selection. Scientific Reports, 11(1): 1-13. DOI:10.1038/s41598-021-82925-7
  • Ormsby, M.J., Caws, T., Burchmore, R., Wallis, T., Verner-Jeffreys, D.W., & Davies, R.L. (2016). Yersinia ruckeri isolates recovered from diseased Atlantic Salmon (Salmo salar) in Scotland are more diverse than those from rainbow trout (Oncorhynchus mykiss) and represent distinct subpopulations. Applied and Environmental Microbiology, 82(19), 5785-5794. DOI:10.1128/AEM.01173-16
  • Pacha, R.E., & Kiehn, E.D. (1969). Characterization and relatedness of marine Vibrios pathogenic to fish: physiology, serology, and epidemiology. Journal of Bacteriology, 100(3), 1242-1247. DOI:10.1128/jb.100.3.1242-1247.1969
  • Pedersen, K., Grisez, L., Van Houdt, R., Tiainen, T., Ollevier, F., & Larsen, J.L. (1999). Extended serotyping scheme for Vibrio anguillarum with the definition and characterization of seven provisional O-serogroups. Current Microbiology, 38(3), 183-189. DOI:10.1007/pl00006784
  • Rabb, L., Cornick, J. W., & McDermott, L.A. (1964). A macroscopic-slide agglutination test for the presumptive diagnosis of furunculosis in fish. The Progressive Fish-Culturist, 26(3), 118-120. DOI:10.1577/1548-8640(1964)26[118:amatft]2.0.co;2
  • Romalde, J.L., & Toranzo, A.E. (2002). Molecular approaches for the study and diagnosis of salmonid Streptococcosis. In C. O. Cunningham, Molecular Diagnosis of Salmonid Diseases (pp. 211-233). Dordrecht, Springer. DOI:10.1007/978-94-017-2315-2_8
  • Romalde, J.L., Magarinos, B., Barja, J.L., & Toranzo, A.E. (1993). Antigenic and molecular characterization of Yersinia ruckeri proposal for a new intraspecies classification. Systematic and Applied Microbiology, 16(3), 411-419. DOI:10.1016/S0723-2020(11)80274-2
  • Romalde, J.L., Magarinos, B., Fouz, B., Bandin, I., Nunez, S., & Toranzo, A.E. (1995). Evaluation of BIONOR Mono-kits for rapid detection of bacterial fish pathogens. Diseases of Aquatic Organisms, 21(1), 25-34. DOI:10.3354/dao021025
  • Romalde, J.L., Planas, E., Sotelo, J.M., & Toranzo, A.E. (2003). First description of Yersinia ruckeri serotype O2 in Spain. Bulletin European Association of Fish Pathologists, 23(3): 135-138.
  • Sørensen, U.B., & Larsen, J.L. (1986). Serotyping of Vibrio anguillarum. Applied and Environmental Microbiology, 51(3), 593-597. DOI: 10.1128/aem.51.3.593-597.1986
  • Stevenson, R.M.W., & Airdrie, D.W. (1984). Serological variation among Yersinia ruckeri strains. Journal of Fish Diseases, 7(4), 247-254. DOI:10.1111/j.1365-2761.1984.tb00930.x
  • Tanrikul, T.T. (2007). Vibriosis as an epizootic disease of rainbow trout (Onchorynchus mykiss) in Turkey. Pakistan Journal of Biological Sciences, 10(10), 1733-1737. DOI:10.3923/pjbs.2007.1733.1737
  • Toranzo, A.E. (2004). Report about fish bacterial diseases. In P. Alvarez-Pellitero, J.L. Barja, B. Basurco, F. Berthe, & A.E. Toranzo (Eds.), Mediterranean Aquaculture Laboratories (pp. 49-89). Zaragoza: CHIEM. (Options Méditerranéennes: Série B. Etudes et Recherches; n. 49).
  • Toranzo, A.E., & Barja, J.L. (1990). A review of the taxonomy and seroepizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain. Diseases of aquatic organisms, 9(1), 73-82. DOI:10.3354/dao009073
  • Toranzo, A.E., Baya, A.M., Roberson, B.S., Barja, J.L., Grimes, D.J., & Hetrick, F.M. (1987). Specificity of slide agglutination test for detecting bacterial fish pathogens. Aquaculture, 61(2), 81-97. DOI:10.1016/0044-8486(87)90361-9
  • Toranzo, A.E., Magariños, B., & Romalde, J.L. (2005). A review of the main bacterial fish diseases in mariculture systems. Aquaculture, 246(1-4): 37-61. DOI:10.1016/j.aquaculture.2005.01.002
  • Toranzo, A.E., Romalde, J. L., Magariños, B., & Barja, J.L. (2009). Present and future of aquaculture vaccines against fish bacterial diseases. In C. Rogers, & B. Basurco (Eds.), The Use of Veterinary Drugs and Vaccines in Mediterranean Aquaculture (pp. 155-176). Zaragoza, CIHEAM. (Options Méditerranéennes: Série A. Séminaires Méditerranéens n. 86)
  • Toranzo, A.E., Magariños, B., & Avendaño-Herrera, R., (2017). Vibriosis: Vibrio anguillarum, V. ordalii and Aliivibrio salmonicida. In P.T.K. Woo, & Cipriano R.C. (Eds.), Fish Viruses and Bacteria: Pathobiology and Protection (pp. 314-333). CABI (CAB International). DOI:10.1079/9781780647784.0314
  • Ürkü, Ç., & Timur, G. (2014). A comparative study of detection methods for Lactococcus garvieae in experimentally infected rainbow trout (Oncorhynchus mykiss, W.). The Israeli Journal of Aquaculture-Bamidgeh, 66, 10 p. DOI:10.46989/001c.20776
  • Wheeler, R.W., Davies, R.L., Dalsgaard, I., Garcia, J., Welch, T.J., Wagley, S., Bateman, K.S., & Verner-Jeffreys D.W. (2009). Yersinia ruckeri biotype 2 isolates from mainland Europe and the UK likely represent different clonal groups. Diseases of Aquatic Organisms, 84(1), 25-33. DOI:10.3354/dao02039
  • Yoshida, T., Endo, M., Sakai, M., & Inglis, V.A. (1997). A Cell capsule with possible involvement in resistance to opsonophagocytosis in Enterococcus seriolicida isolated from yellowtail Seriola quinqueradiata. Diseases of Aquatic Organisms, 29, 233-235. DOI:10.3354/dao029233
  • Yoshida, T., Eshima, T., Wada, Y., Yamada, Y., Kakizaki, E., Sakai, M., Kitao, T., & Inglis, V. (1996). Phenotypic variation associated with an anti-phagocytic factor in the bacterial fish pathogen Enterococcus seriolicida. Diseases of Aquatic Organisms, 25(1-2), 81-86. DOI:10.3354/dao025081

Lam aglütinasyon metodu ile bakteriyel balık hastalıklarının teşhisi ve serotiplerin sınıflandırılması

Year 2022, Volume: 39 Issue: 2, 97 - 103, 15.06.2022
https://doi.org/10.12714/egejfas.39.2.02

Abstract



Bakteriyel balık patojenleri gökkuşağı alabalığı yetiştiriciliği yapan balık çiftliklerinde ciddi kayıplara sebep olmaktadır. Su ürünleri yetiştiriciliğinde hastalık etkeni bakteriyel patojenler, oluşan hastalıkların teşhisi için hızlı teşhis yöntemlerinin gelişmesine yol açmıştır. Hastalıklar yetiştiricilik tesislerinde görüldükten sonra balık patojenlerinin hızlı teşhis edilmesi, hastalıkların tedavi edilerek oluşacak ekonomik kayıpların önüne geçilmesi için önemlidir. Balık hastalıklarının hızlı teşhisinde az miktarda antiserum ve örnek ile uygulanabilen serolojik teknikler tercih edilmektedir. Bu çalışmada Lactococcus garvieae, Vibrio (Listonella) anguillarum ve Yersinia ruckeri’nin referans suşlarından antijenler kullanılmak üzere formalin ile inaktive edilerek, Yeni Zelanda tavşanlarına ardışık dozlarda intravenöz enjeksiyonlar gerçekleştirilmiştir. Son enjeksiyon yapıldıktan bir hafta sonra antiserum elde edilmiştir. 18 Y. ruckeri, 14 V. anguillarum ve 7 L. garvieae ve referans (ATCC 43305, ATCC 29473, ATCC 49156) suşlar dahil olmak üzere toplamda 42 suş ile çalışma yapılmıştır. Lam aglütinasyon metodu ile incelenen V. anguillarum suşlarında baskın olarak (%86) serotip O1, Y. ruckeri suşlarında (%84) serotip O1 tespit edilmiştir. L. garvieae suşlarının Japon KG- antiserumu ile aglütinasyon vermediği, ancak Türk KG- antiserumu ile pozitif reaksiyon oluşturduğu tespit edilmiştir.


References

  • Altun, S., Onuk, E.E., Ciftci, A., Duman, M., & Büyükekiz, A.G. (2013). Determination of phenotypic, serotypic and genetic diversity and antibiotyping of Yersinia ruckeri isolated from rainbow trout. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(2), 225-232. DOI: 10.9775/kvfd.2012.7606
  • Austin, B., & Newaj-Fyzul, A. (2017). Diagnosis and control of diseases of fish and shellfish. John Wiley and Sons, Chichester, UK. DOI:10.1002/9781119152125
  • Avsever, M.L., & Ün, C. (2015). Distribution of hemolysin genes in Turkish Vibrio anguillarum isolates. Bulletin of the European Association of Fish Pathologists, 35(3), 75-84.
  • Bachrach, G., Zlotkin, A., Hurvitz, A., Evans, D.L., & Eldar, A. (2001). Recovery of Streptococcus iniae from diseased fish previously vaccinated with a Streptococcus vaccine. Applied and Environmental Microbiology, 67(8), 3756-3758.DOI:10.1128/AEM.67.8.3756-3758.2001
  • Balta, F., & Dengiz Balta, Z. (2017). Doğu Karadeniz’de yetiştiriciliği yapılan gökkuşağı alabalıkları (Oncorhynchus mykiss)’ndan izole edilen Vibrio anguillarum suşlarının serotiplendirilmesi, genetik karakterizasyonu ve antimikrobiyal duyarlılığının belirlenmesi. Ankara Universitesi Veteriner Fakultesi Dergisi, 64, 321-328. DOI:10.1501/Vetfak_0000002816
  • Balta, F., Sandalli, C., Kayis, S., & Ozgumus, O.B. (2010). Molecular analysis of antimicrobial resistance in Yersinia ruckeri strains isolated from rainbow trout (Oncorhynchus mykiss) grown in commercial fish farms in Turkey. Bulletin of the European Association of Fish Pathologists, 30(6), 211-219.
  • Balta, F., Dengiz Balta, Z., Özgümüş, O.B., & Çağırgan, H. (2016). Doğu Karadeniz Bölgesi’ndeki gökkuşağı alabalığı (Oncorhynchus mykiss) çiftliklerinde Yersinia ruckeri’nin portörlük yönünden tetkiki ve antimikrobiyal direncin tespiti. Journal of Anatolian Environmental & Animal Sciences, 1(3), 72-76. DOI:10.35229/jaes.280741
  • Barnes, A.C., & Ellis, A.E. (2004). Role of capsule in serotypic differences and complement fixation by Lactococcus garvieae. Fish and Shellfish Immunology, 16(2), 207-214. DOI:10.1016/s1050-4648(03)00079-2
  • Barnes, A.C., Guyot, C., Hansen, B. G., Mackenzie, K., Horne, M.T., & Ellis, A.E. (2002). Resistance to serum killing may contribute to differences in the abilities of capsulate and non-capsulated isolates of Lactococcus garvieae to cause disease in rainbow trout (Oncorhynchus mykiss L.). Fish and Shellfish Immunology, 12(2), 155-168. DOI:10.1006/fsim.2001.0361
  • Bastardo, A., Bohle, H., Ravelo, C., Toranzo, A.E., & Romalde, J.L. (2011). Serological and molecular heterogeneity among Yersinia ruckeri strains isolated from farmed Atlantic salmon Salmo salar in Chile. Diseases of Aquatic Organisms, 93(3), 207-214. DOI:10.3354/dao02296
  • Çağırgan, H. (2004). Biotyping of Lactococcus garvieae isolated from Turkey. Su Ürünleri Dergisi, 21(3), 267-269.
  • Davies, R.L. (1990). O-serotyping of Yersinia ruckeri with special emphasis on European isolates. Veterinary Microbiology, 22(4), 299-307. DOI:10.1016/0378-1135(90)90016-O
  • Eldar, A., Ghittino, C., Asanta, L., Bozzetta, E., Goria, M., Prearo, M., & Bercovier, H. (1996). Enterococcus seriolicida is a junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningoencephalitis in fish. Current Microbiology, 32(2), 85-88. DOI:10.1007/s002849900015
  • Eurell, T.E., Lewis, D. H., & Grumbles, L.C. (1979). Stained Bacterial Antigens for Use in Microagglutination Procedures. The Progressive Fish-Culturist, 41(2), 55-57.DOI:10.1577/1548-8659(1979)41[55:sbafui]2.0.co;2
  • Grisez, L., & Ollevier, F. (1995). Comparative serology of the marine fish pathogen Vibrio anguillarum. Applied and Environmental Microbiology, 61(12), 4367-4373. DOI:10.1128/aem.61.12.4367-4373.1995
  • Hansen, M.J., Kudirkiene, E., & Dalsgaard, I. (2020). Analysis of 44 Vibrio anguillarum genomes reveals high genetic diversity. PeerJ, 8, e10451. DOI:10.7717/peerj.10451
  • Hickey, M. E., & Lee, J.L. (2017). A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention. Reviews in Aquaculture, 10(3), 585-610. DOI:10.1111/raq.12188
  • Kang, S.H., Shin, G.W., Shin, Y.S., Palaksha, K.J., Kim, Y.R., Yang, H.H., Lee, E.R., Lee, E.G., Huh, N.E., Ju, O.M., & Jung, T.S. (2004). Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish (Sebastes schlegeli). Journal of Veterinary Science, 5(4), 387-390. DOI:10.4142/jvs.2004.5.4.387
  • Kitao, T. (1982). The methods for detection of Streptococcus sp., causative bacteria of streptococcal disease of cultured yellowtail (Seriola quinqueradiata) especially, their cultural, biochemical and serological properties. Fish Pathology, 17(1), 17-26. DOI:10.3147/jsfp.17.17
  • Kitao, T., Aoki, T., Fukudome, M., Kawano, K., Wada, Y., & Mizuno, Y. (1983). Serotyping of Vibrio anguillarum isolated from diseased freshwater fish in Japan. Journal of Fish Diseases, 6(2), 175-181. DOI:10.1111/j.1365-2761.1983.tb00064.x
  • Kitao, T., Aoki, T., & Muroga, K., (1984). Three new O-serotypes of Vibrio anguillarum [isolated from ayu, Plecoglossus altivelis]. Bulletin of the Japanese Society of Scientific Fisheries (Japan), 50(11), 1955.
  • Larsen, J.L. (1990). Vibrio anguillarum: characterization, ecology and pathogenicity. Doctoral dissertation in veterinary scicences, The Royal Veterinary and Agricultural University.
  • Larsen, J.L., & Olsen, J.E. (1991). Occurrence of plasmids in Danish isolates of Vibrio anguillarum serovars O1 and O2 and association of plasmids with phenotypic characteristics. Applied and Environmental Microbiology, 57(8), 2158-2163. DOI:10.1128/aem.57.8.2158-2163.1991
  • Leblanc, D., Mittal, K.R., Olivier, G., & Lallier, R. (1981). Serogrouping of motile Aeromonas species isolated from healthy and moribund fish. Applied and Environmental Microbiology, 42(1), 56-60. DOI:10.1128/aem.42.1.56-60.1981
  • Morita, H., Toh, H., Oshima, K., Yoshizaki, M., Kawanishi, M., Nakaya, K., Suzuki, T., Miyauchi, E., Ishii, Y., Tanabe, S., Murakami, M., & Hattori, M. (2011). Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae. PLoS One, 6(8), e23184. DOI:10.1371/journal.pone.0023184
  • Nakai, T., Muroga, K., & Wakabayashi, H. (1981). Serological properties of Pseudomonas anguilliseptica in agglutination. Bulletin of the Japanese Society of Scientific Fisheries, 47(6), 699-703. DOI:10.2331/suisan.47.699
  • Nomura, J., & Aoki, T. (1985). Morphological analysis of lipopolysaccharide from gram-negative fish pathogenic bacteria. Fish Pathology, 20(2-3), 193-197. DOI:10.3147/jsfp.20.193
  • Oinaka, D., Yoshimura, N., Fukuda, Y., Yamashita, A., Urasaki, S., Wada, Y., & Yoshida, T. (2015). Isolation of Lactococcus garvieae showing no agglutination with anti-KG-phenotype rabbit serum. Fish Pathology, 50(2), 37-43. DOI:10.3147/jsfp.50.37
  • Ooyama, T., Hirokawa, Y., Minami, T., Yasuda, H., Nakai, T., Endo, M., Ruangpan, L., & Yoshida, T. (2002). Cell-surface properties of Lactococcus garvieae strains and their immunogenicity in the yellowtail Seriola quinqueradiata. Diseases of Aquatic Organisms, 51(3): 169-177. DOI:10.3354/dao051169
  • Ormsby, M.J., & Davies, R.L. (2021). Diversification of OmpA and OmpF of Yersinia ruckeri is independent of the underlying species phylogeny and evidence of virulence-related selection. Scientific Reports, 11(1): 1-13. DOI:10.1038/s41598-021-82925-7
  • Ormsby, M.J., Caws, T., Burchmore, R., Wallis, T., Verner-Jeffreys, D.W., & Davies, R.L. (2016). Yersinia ruckeri isolates recovered from diseased Atlantic Salmon (Salmo salar) in Scotland are more diverse than those from rainbow trout (Oncorhynchus mykiss) and represent distinct subpopulations. Applied and Environmental Microbiology, 82(19), 5785-5794. DOI:10.1128/AEM.01173-16
  • Pacha, R.E., & Kiehn, E.D. (1969). Characterization and relatedness of marine Vibrios pathogenic to fish: physiology, serology, and epidemiology. Journal of Bacteriology, 100(3), 1242-1247. DOI:10.1128/jb.100.3.1242-1247.1969
  • Pedersen, K., Grisez, L., Van Houdt, R., Tiainen, T., Ollevier, F., & Larsen, J.L. (1999). Extended serotyping scheme for Vibrio anguillarum with the definition and characterization of seven provisional O-serogroups. Current Microbiology, 38(3), 183-189. DOI:10.1007/pl00006784
  • Rabb, L., Cornick, J. W., & McDermott, L.A. (1964). A macroscopic-slide agglutination test for the presumptive diagnosis of furunculosis in fish. The Progressive Fish-Culturist, 26(3), 118-120. DOI:10.1577/1548-8640(1964)26[118:amatft]2.0.co;2
  • Romalde, J.L., & Toranzo, A.E. (2002). Molecular approaches for the study and diagnosis of salmonid Streptococcosis. In C. O. Cunningham, Molecular Diagnosis of Salmonid Diseases (pp. 211-233). Dordrecht, Springer. DOI:10.1007/978-94-017-2315-2_8
  • Romalde, J.L., Magarinos, B., Barja, J.L., & Toranzo, A.E. (1993). Antigenic and molecular characterization of Yersinia ruckeri proposal for a new intraspecies classification. Systematic and Applied Microbiology, 16(3), 411-419. DOI:10.1016/S0723-2020(11)80274-2
  • Romalde, J.L., Magarinos, B., Fouz, B., Bandin, I., Nunez, S., & Toranzo, A.E. (1995). Evaluation of BIONOR Mono-kits for rapid detection of bacterial fish pathogens. Diseases of Aquatic Organisms, 21(1), 25-34. DOI:10.3354/dao021025
  • Romalde, J.L., Planas, E., Sotelo, J.M., & Toranzo, A.E. (2003). First description of Yersinia ruckeri serotype O2 in Spain. Bulletin European Association of Fish Pathologists, 23(3): 135-138.
  • Sørensen, U.B., & Larsen, J.L. (1986). Serotyping of Vibrio anguillarum. Applied and Environmental Microbiology, 51(3), 593-597. DOI: 10.1128/aem.51.3.593-597.1986
  • Stevenson, R.M.W., & Airdrie, D.W. (1984). Serological variation among Yersinia ruckeri strains. Journal of Fish Diseases, 7(4), 247-254. DOI:10.1111/j.1365-2761.1984.tb00930.x
  • Tanrikul, T.T. (2007). Vibriosis as an epizootic disease of rainbow trout (Onchorynchus mykiss) in Turkey. Pakistan Journal of Biological Sciences, 10(10), 1733-1737. DOI:10.3923/pjbs.2007.1733.1737
  • Toranzo, A.E. (2004). Report about fish bacterial diseases. In P. Alvarez-Pellitero, J.L. Barja, B. Basurco, F. Berthe, & A.E. Toranzo (Eds.), Mediterranean Aquaculture Laboratories (pp. 49-89). Zaragoza: CHIEM. (Options Méditerranéennes: Série B. Etudes et Recherches; n. 49).
  • Toranzo, A.E., & Barja, J.L. (1990). A review of the taxonomy and seroepizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain. Diseases of aquatic organisms, 9(1), 73-82. DOI:10.3354/dao009073
  • Toranzo, A.E., Baya, A.M., Roberson, B.S., Barja, J.L., Grimes, D.J., & Hetrick, F.M. (1987). Specificity of slide agglutination test for detecting bacterial fish pathogens. Aquaculture, 61(2), 81-97. DOI:10.1016/0044-8486(87)90361-9
  • Toranzo, A.E., Magariños, B., & Romalde, J.L. (2005). A review of the main bacterial fish diseases in mariculture systems. Aquaculture, 246(1-4): 37-61. DOI:10.1016/j.aquaculture.2005.01.002
  • Toranzo, A.E., Romalde, J. L., Magariños, B., & Barja, J.L. (2009). Present and future of aquaculture vaccines against fish bacterial diseases. In C. Rogers, & B. Basurco (Eds.), The Use of Veterinary Drugs and Vaccines in Mediterranean Aquaculture (pp. 155-176). Zaragoza, CIHEAM. (Options Méditerranéennes: Série A. Séminaires Méditerranéens n. 86)
  • Toranzo, A.E., Magariños, B., & Avendaño-Herrera, R., (2017). Vibriosis: Vibrio anguillarum, V. ordalii and Aliivibrio salmonicida. In P.T.K. Woo, & Cipriano R.C. (Eds.), Fish Viruses and Bacteria: Pathobiology and Protection (pp. 314-333). CABI (CAB International). DOI:10.1079/9781780647784.0314
  • Ürkü, Ç., & Timur, G. (2014). A comparative study of detection methods for Lactococcus garvieae in experimentally infected rainbow trout (Oncorhynchus mykiss, W.). The Israeli Journal of Aquaculture-Bamidgeh, 66, 10 p. DOI:10.46989/001c.20776
  • Wheeler, R.W., Davies, R.L., Dalsgaard, I., Garcia, J., Welch, T.J., Wagley, S., Bateman, K.S., & Verner-Jeffreys D.W. (2009). Yersinia ruckeri biotype 2 isolates from mainland Europe and the UK likely represent different clonal groups. Diseases of Aquatic Organisms, 84(1), 25-33. DOI:10.3354/dao02039
  • Yoshida, T., Endo, M., Sakai, M., & Inglis, V.A. (1997). A Cell capsule with possible involvement in resistance to opsonophagocytosis in Enterococcus seriolicida isolated from yellowtail Seriola quinqueradiata. Diseases of Aquatic Organisms, 29, 233-235. DOI:10.3354/dao029233
  • Yoshida, T., Eshima, T., Wada, Y., Yamada, Y., Kakizaki, E., Sakai, M., Kitao, T., & Inglis, V. (1996). Phenotypic variation associated with an anti-phagocytic factor in the bacterial fish pathogen Enterococcus seriolicida. Diseases of Aquatic Organisms, 25(1-2), 81-86. DOI:10.3354/dao025081

Details

Primary Language English
Journal Section Articles
Authors

Kaan KUMAŞ 0000-0003-1583-8482

Prof.dr. T. Tansel TANRIKUL 0000-0002-1684-7965

Project Number 2021-TYL-FEBE-0012
Publication Date June 15, 2022
Submission Date January 21, 2022
Published in Issue Year 2022Volume: 39 Issue: 2

Cite

APA KUMAŞ, K., & TANRIKUL, P. T. T. (2022). Diagnosis of bacterial fish diseases and classification of serotypes with slide agglutination method. Ege Journal of Fisheries and Aquatic Sciences, 39(2), 97-103. https://doi.org/10.12714/egejfas.39.2.02