Bacterial fish pathogens cause significant losses in rainbow trout farms. In fish farms, bacterial pathogens cause threatening diseases which have made necessary to develop rapid methods for disease diagnosis. Serological techniques which are applied with a small amount of antiserum and sample, are preferred for the rapid diagnosis of fish diseases. In this study, formalin-killed antigens prepared from reference strains of Lactococcus garvieae, Yersinia ruckeri, and Vibrio (Listonella) anguillarum were injected intravenously in consecutive doses to New Zealand rabbits. One week after the last injection, the sera separated to use in the slide agglutination tests. A total of 42 strains were studied, including Y. ruckeri (18 isolates), V. anguillarum (14 isolates), and L. garvieae (7 isolates) and 3 reference (ATCC 43305, ATCC 29473, ATCC 49156) strains. Serotype O1 determined predominant serotype (86%) in V. anguillarum and Y. ruckeri (84%) strains examined by slide agglutination method. L. garvieae strains did not react against Japanese antisera but positively reacted against Turkish L. garvieae antisera.
Izmir Katip Celebi University Scientific Research Projects Coordinatorship
This study presents a part of Kaan KUMAŞ’s master thesis titled “Diagnosis of bacterial fish diseases with using slide agglutination method” (with English abstract) and was supported by İzmir Katip Çelebi University Scientific Research Projects Coordinatorship with the project number 2021-TYL-FEBE-0012.