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Vitamins A, E, C, β -carotene contents and MDA level of freshwater mussel, (Unio elongatulus eucirrus Bourguignat 1860) in the Karakaya Dam Lake

Year 2022, Volume: 39 Issue: 2, 120 - 124, 15.06.2022
https://doi.org/10.12714/egejfas.39.2.05

Abstract



In this study, we aimed to determine the effect of domestic and agricultural discharge on the level of antioxidant vitamins A, E, C, β-carotene and malondialdehyde (MDA, an indicator of oxidative stress) in the muscle tissue of freshwater mussel as known bioindicator (Unio elongatulus eucirrus). The freshwater mussels were collected from Arguvan (uncontaminated reference site) and Battalgazi (exposed to discharge site) in the Karakaya Dam Lake. In order to examine the effect of this discharge on freshwater mussel collected from these two different regions, antioxidant vitamins A, E, C, β carotene and Malondialdehyde (MDA) levels were analyzed using High Performance Liquid Chromatography (HPLC). The comparison between groups from the different localities showed that concentrations of the vitamins A, E, C, β-carotene and MDA were found statistically significant according to between the localities (p<0.05) The results showed that vitamins levels were higher and MDA levels were lower in the reference site.

References

  • Aksul, Ö., Yabanli, M., Can, E., Kutluyer, F., Kehayias, G., Can, Ş.S., Kocabaş, M., & Demir, V. (2012). Comparison of heavy metals bioaccumulation by Dreissena polymorpha (Pallas, 1771) and Unio elongatulus eucirrus (Bourguıgnat, 1860) from Keban Dam Lake, Turkey. Fresenius Environmental Bulletin, Volume 21-(7): 1942-1947.
  • Barım, Ö., & Karatepe, M., (2010). The effects of pollution on the vitamins A, E, C, b-carotene contents and oxidative stress of the freshwater crayfish, Astacus leptodactylus. Ecotoxicology and Environmental Safety, 73: 138–142. DOI:10.1016/j.ecoenv.2009.08.002
  • Bolognesi, C., Rabboni, R., & Roggieri, P. (1996). Genotoxicity biomarkers in M. galloprovincialis as indicators of marine pollutants. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 113(2), 319–323. DOI:10.1016/B978-044482913-9/50005-5
  • Borkovic-Mitic, S., Pavlovic, S., Perendijaa, B., Despotovic, S., Gavric, J., Gaci, Z., & Saici, Z. (2013). Influence of some metal concentrations on the activity of antioxidant enzymes and concentrations of vitamin E and SH-groups in the digestive gland and gills of the freshwater bivalve Unio tumidus from the Serbian part of Sava River. Ecological Indicators, 32, 212-221. DOI:10.1016/j.ecolind.2013.03.024
  • Box, A., Sureda, A., Galgani F., Pons, A., & Deudero, S. (2007). Assessment of environmental pollution at balearic ıslands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis. Comparative Biochemistry and Physiology Part C, 146: 531–539. DOI: 10.1016/j.cbpc.2007.06.006
  • Catignani, G.L., & Bieri, J.G. (1983). Simultaneous determination of retinol and α-tocopherol in serum or plazma by liquid chromatography. Clinical Chemistry, 29(4), 708–712. DOI:10.1093/clinchem/29.4.708
  • Chandurvelan, R., Marsden, I. D., Gaw, S., & Glover, C. N. (2013). Biochemical biomarker responses of green-lipped mussel, Perna canaliculus, to acute and subchronic waterborne cadmium toxicity. Aquatic Toxicology, (140-141), 303– 313. DOI:10.1016/j.aquatox.2013.06.015
  • Cossu, C., Doyotte, A., Babut, M., Exinger, A., & Vasseur, P. (2000). Antioxidant biomarkers in freshwater bivalves, Unio tumidus, in response to different contamination profiles of aquatic sediments. Ecotoxicology and Environmental Safety, 45, 106-121. DOI:10.1006/eesa.1999.1842
  • Diplock, A. (1998). Healthy lifestyles nutrition and physical activity: Antioxidant nutrients. ILSI Europe Concise Monograph Series, p. 59, Belgium. DOI:10.3109/09637489809086430
  • Dyrynda, E.A., Pipe, R.K. Burt, G.R., & Ratcliffe, N.A. (1998). Modulations in the immune defences of mussels (Mytilus edulis) from contaminated sites in the UK. Aquatic Toxicology, 42(3), 169–185. DOI:10.1017/S0025315400033993
  • Ekin, İ., Başhan, M., & Şeşen,R., (2010). Fatty acid composition of neutral lipid, phospholipid and total lipid of freshwater mussel (Unio elongatulus Bourguignat, 1860) collected from Tigris River AKÜ Fen Bilimleri Dergisi 77-84. (only English abstract).
  • Ergüden, C. (2012). The effects of water quality and organochlorine pesticides on the distribution of mussels (Mytilus Galloprovincialis Lamarck, 1819) in Izmir Bay. (Master Thesis). Dokuz Eylul University Graduate School of Natural and Applied Sciences, Department of Living Marine Resources, Department of Marine Sciences and Technology, (only English abstract).
  • Esen, Ö. (2006). Investigation of toxic materials that analised in black mussel Mytilus galloprovincıalis Lamarck in 1819 in Izmir Bay. (Master Thesis). Ege University, Institute of Science and Technology, (only English abstract).
  • Faria, M., Ochoa V., Blázquez, M., San, Juan M.F., Lazzara, R., Lacorte, S., Soares, A., & Barata, C. (2014). Separating natural from anthropogenic causes of impairment in zebra mussel (Dreissena polymorpha) populations living across a pollution gradient. Aquatic Toxicology, 152- 82–95. DOI:10.1016/j.aquatox.2014.03.020
  • Halliwell, B., & Gutteridge J.M.C. (2000). Free radicals in biology and medicine. Oxford University Press.NewYork, USA.107 208. DOI:10.1093/acprof:oso/9780198717478. 001.0001
  • İriş, C., & Çinar, M., (2019). Antioxidant vitamins and its effects on heavy metal toxicity. Bulletin of Veterinary Pharmacology and Toxicology Association, 10 (3), 135-151, (only English abstract)
  • Kanar, D. (2012). Monitoring of heavy metal pollution on Tuzla (Istanbul) coast with mussels. (Master Thesis). Marmara University, Institute of Science, Department of Environmental Sciences, (only English abstract).
  • Karatepe, M. (2004). Simultaneous determination of ascorbic acid and free malondialdehyde in humasem by HPLC/UV. LC-GC North America, 22, 362- 365.
  • Kizilkaya, B., Acarli, S., Ertuğrul , P.V., Selçuk Berber, S., & Çelik, V. (2021). Variation in radical antioxidant capacity and the total amount of carotenoids in razor clams, Ensis marginatus (Pennant, 1777), from the Çanakkale Strait (Abidealtı), Turkey. International Journal of Oceanography and Hydrobiology, 50 (1), 16-23. DOI: 10.2478/oandhs-2021-0002
  • Lam, P.K.S., Cheung, C.C.C., Zheng, G.J., Li, A.M.Y., & Richardson, B.J. (2001). Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic Toxicology, 52, 189-203. DOI: 10.1016/s0166-445x(00)00145-4
  • Narbonne, J.F., Aarab, N., Clérandeau, C., Daubèze, M., Narbonne, J., Champeau, & O., Garrigues, P. (2005). Scale of classification based on biochemical markers in mussels: application to pollution monitoring in Mediterranean coasts and temporal trends. Biomarkers, 10(1), 58-71. DOI:10.1080/13547500500071339
  • Özen, M., (2018). Karakaya Barajı. https://www.dogadernegi.org/karakaya-baraji/ (26.09.2021).
  • Pipe, R., Coles, J., Carissan, F.M., & Ramanathan, K. (1999). Copper induced immunomodulation in the marine mussel, Mytilus edulis. Aquatic Toxicology, 46(1), 43–54. DOI:10.1016/S0166-445X(98)00114-3
  • Pytharopoulou, S., Sazakli, E., G., Grintzalis, K., Georgiou, C.D., Leotsinidis, M., Dimitrios & Kalpaxis, D.L. (2008). Translational responses of Mytilus galloprovincialis to environmental pollution: Integrating the responses to oxidative stress and other biomarker responses into a general stress index. Aquatic Toxicology, 89(1), 18-27. DOI:10.1016/j.aquatox.2008.05.013
  • Ribera, J.F., Narbonne, X., Michel, D.R., Livingstone, S., & Hara, O. (1991). Responses of antioxidants and lipid peroxidation in mussels to oxidative damage exposure. Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology, 100(1-2), 177-81. DOI:10.1016/0742-8413(91)90149-n
  • Şahin, A.G, Sünbül, M.R., & Küçükyılmaz, M. (2016). Determination of heavy metal levels in freshwater mussels (Unio elengatulus eucirrus Bourguignat 1860) in Karakaya Dam Lake Fırat Üniversitesi, Fen Bilimleri Dergisi, 28 (1), 13-19, (only English abstract)
  • Vlahogianni, T., Dassenakis, M., Scoullos, M.J., & Valavanidis, A. (2007). Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussels Mytilus galloprovincialis for assessing heavy metals pollution in coastal areas from the Saronikos Gulf of Greece. Marine Pollution Bulletin, 54, 1361-1371. DOI:10.1016/j.marpolbul.2007.05.018
  • Yalçın, M. (2006). Effect of environmental factors on growth, biochemical parameters and condition index in freshwater mussel (Unio pictorum Linnaeus, 1758). (Master Thesis) Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, Çanakkale, 59s, (only English abstract).

Keban Baraj Gölü tatlı su midyesi (Unio elongatulus eucirrus Bourguignat 1860)’nin, A, E, C vitamini, β karatone içeriği ve MDA değerleri

Year 2022, Volume: 39 Issue: 2, 120 - 124, 15.06.2022
https://doi.org/10.12714/egejfas.39.2.05

Abstract



Bu çalışmada, biyoindikatör (Unio elongatulus eucirrus) olarak bilinen tatlı su midyesinin kas dokusunda evsel ve tarımsal deşarjın antioksidan vitaminler A, E, C, β karoten ve malondialdehit (MDA, oksidatif stresin bir göstergesi) düzeyine etkisini belirlemeyi amaçladık. Tatlı su midye örnekleri Karakaya Baraj Gölü'ndeki Arguvan (kirlenmemiş referans alanı) ve Battalgazi'den (deşarj alanına maruz kalan) toplanmıştır. Bu deşarjın bu iki farklı bölgeden toplanan tatlı su midyesi numuneleri üzerindeki etkisini incelemek için, antioksidan vitaminler A, E, C, β karoten ve Malondialdehit (MDA) seviyeleri Yüksek Performanslı Sıvı Kromatografisi (HPLC) kullanılarak analiz edildi. Bölgeler arası yapılan karşılaştırmada tatlı su midye dokularında tespit edilen antioksidan vitaminler ve MDA değişimleri arasında istatistiki olarak önemli bir farkın olduğu belirlenmiştir (p<0,05). Sonuçlar, referans bölgede vitamin seviyelerinin daha yüksek ve MDA seviyesinin daha düşük olduğunu gösterdi.



References

  • Aksul, Ö., Yabanli, M., Can, E., Kutluyer, F., Kehayias, G., Can, Ş.S., Kocabaş, M., & Demir, V. (2012). Comparison of heavy metals bioaccumulation by Dreissena polymorpha (Pallas, 1771) and Unio elongatulus eucirrus (Bourguıgnat, 1860) from Keban Dam Lake, Turkey. Fresenius Environmental Bulletin, Volume 21-(7): 1942-1947.
  • Barım, Ö., & Karatepe, M., (2010). The effects of pollution on the vitamins A, E, C, b-carotene contents and oxidative stress of the freshwater crayfish, Astacus leptodactylus. Ecotoxicology and Environmental Safety, 73: 138–142. DOI:10.1016/j.ecoenv.2009.08.002
  • Bolognesi, C., Rabboni, R., & Roggieri, P. (1996). Genotoxicity biomarkers in M. galloprovincialis as indicators of marine pollutants. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 113(2), 319–323. DOI:10.1016/B978-044482913-9/50005-5
  • Borkovic-Mitic, S., Pavlovic, S., Perendijaa, B., Despotovic, S., Gavric, J., Gaci, Z., & Saici, Z. (2013). Influence of some metal concentrations on the activity of antioxidant enzymes and concentrations of vitamin E and SH-groups in the digestive gland and gills of the freshwater bivalve Unio tumidus from the Serbian part of Sava River. Ecological Indicators, 32, 212-221. DOI:10.1016/j.ecolind.2013.03.024
  • Box, A., Sureda, A., Galgani F., Pons, A., & Deudero, S. (2007). Assessment of environmental pollution at balearic ıslands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis. Comparative Biochemistry and Physiology Part C, 146: 531–539. DOI: 10.1016/j.cbpc.2007.06.006
  • Catignani, G.L., & Bieri, J.G. (1983). Simultaneous determination of retinol and α-tocopherol in serum or plazma by liquid chromatography. Clinical Chemistry, 29(4), 708–712. DOI:10.1093/clinchem/29.4.708
  • Chandurvelan, R., Marsden, I. D., Gaw, S., & Glover, C. N. (2013). Biochemical biomarker responses of green-lipped mussel, Perna canaliculus, to acute and subchronic waterborne cadmium toxicity. Aquatic Toxicology, (140-141), 303– 313. DOI:10.1016/j.aquatox.2013.06.015
  • Cossu, C., Doyotte, A., Babut, M., Exinger, A., & Vasseur, P. (2000). Antioxidant biomarkers in freshwater bivalves, Unio tumidus, in response to different contamination profiles of aquatic sediments. Ecotoxicology and Environmental Safety, 45, 106-121. DOI:10.1006/eesa.1999.1842
  • Diplock, A. (1998). Healthy lifestyles nutrition and physical activity: Antioxidant nutrients. ILSI Europe Concise Monograph Series, p. 59, Belgium. DOI:10.3109/09637489809086430
  • Dyrynda, E.A., Pipe, R.K. Burt, G.R., & Ratcliffe, N.A. (1998). Modulations in the immune defences of mussels (Mytilus edulis) from contaminated sites in the UK. Aquatic Toxicology, 42(3), 169–185. DOI:10.1017/S0025315400033993
  • Ekin, İ., Başhan, M., & Şeşen,R., (2010). Fatty acid composition of neutral lipid, phospholipid and total lipid of freshwater mussel (Unio elongatulus Bourguignat, 1860) collected from Tigris River AKÜ Fen Bilimleri Dergisi 77-84. (only English abstract).
  • Ergüden, C. (2012). The effects of water quality and organochlorine pesticides on the distribution of mussels (Mytilus Galloprovincialis Lamarck, 1819) in Izmir Bay. (Master Thesis). Dokuz Eylul University Graduate School of Natural and Applied Sciences, Department of Living Marine Resources, Department of Marine Sciences and Technology, (only English abstract).
  • Esen, Ö. (2006). Investigation of toxic materials that analised in black mussel Mytilus galloprovincıalis Lamarck in 1819 in Izmir Bay. (Master Thesis). Ege University, Institute of Science and Technology, (only English abstract).
  • Faria, M., Ochoa V., Blázquez, M., San, Juan M.F., Lazzara, R., Lacorte, S., Soares, A., & Barata, C. (2014). Separating natural from anthropogenic causes of impairment in zebra mussel (Dreissena polymorpha) populations living across a pollution gradient. Aquatic Toxicology, 152- 82–95. DOI:10.1016/j.aquatox.2014.03.020
  • Halliwell, B., & Gutteridge J.M.C. (2000). Free radicals in biology and medicine. Oxford University Press.NewYork, USA.107 208. DOI:10.1093/acprof:oso/9780198717478. 001.0001
  • İriş, C., & Çinar, M., (2019). Antioxidant vitamins and its effects on heavy metal toxicity. Bulletin of Veterinary Pharmacology and Toxicology Association, 10 (3), 135-151, (only English abstract)
  • Kanar, D. (2012). Monitoring of heavy metal pollution on Tuzla (Istanbul) coast with mussels. (Master Thesis). Marmara University, Institute of Science, Department of Environmental Sciences, (only English abstract).
  • Karatepe, M. (2004). Simultaneous determination of ascorbic acid and free malondialdehyde in humasem by HPLC/UV. LC-GC North America, 22, 362- 365.
  • Kizilkaya, B., Acarli, S., Ertuğrul , P.V., Selçuk Berber, S., & Çelik, V. (2021). Variation in radical antioxidant capacity and the total amount of carotenoids in razor clams, Ensis marginatus (Pennant, 1777), from the Çanakkale Strait (Abidealtı), Turkey. International Journal of Oceanography and Hydrobiology, 50 (1), 16-23. DOI: 10.2478/oandhs-2021-0002
  • Lam, P.K.S., Cheung, C.C.C., Zheng, G.J., Li, A.M.Y., & Richardson, B.J. (2001). Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic Toxicology, 52, 189-203. DOI: 10.1016/s0166-445x(00)00145-4
  • Narbonne, J.F., Aarab, N., Clérandeau, C., Daubèze, M., Narbonne, J., Champeau, & O., Garrigues, P. (2005). Scale of classification based on biochemical markers in mussels: application to pollution monitoring in Mediterranean coasts and temporal trends. Biomarkers, 10(1), 58-71. DOI:10.1080/13547500500071339
  • Özen, M., (2018). Karakaya Barajı. https://www.dogadernegi.org/karakaya-baraji/ (26.09.2021).
  • Pipe, R., Coles, J., Carissan, F.M., & Ramanathan, K. (1999). Copper induced immunomodulation in the marine mussel, Mytilus edulis. Aquatic Toxicology, 46(1), 43–54. DOI:10.1016/S0166-445X(98)00114-3
  • Pytharopoulou, S., Sazakli, E., G., Grintzalis, K., Georgiou, C.D., Leotsinidis, M., Dimitrios & Kalpaxis, D.L. (2008). Translational responses of Mytilus galloprovincialis to environmental pollution: Integrating the responses to oxidative stress and other biomarker responses into a general stress index. Aquatic Toxicology, 89(1), 18-27. DOI:10.1016/j.aquatox.2008.05.013
  • Ribera, J.F., Narbonne, X., Michel, D.R., Livingstone, S., & Hara, O. (1991). Responses of antioxidants and lipid peroxidation in mussels to oxidative damage exposure. Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology, 100(1-2), 177-81. DOI:10.1016/0742-8413(91)90149-n
  • Şahin, A.G, Sünbül, M.R., & Küçükyılmaz, M. (2016). Determination of heavy metal levels in freshwater mussels (Unio elengatulus eucirrus Bourguignat 1860) in Karakaya Dam Lake Fırat Üniversitesi, Fen Bilimleri Dergisi, 28 (1), 13-19, (only English abstract)
  • Vlahogianni, T., Dassenakis, M., Scoullos, M.J., & Valavanidis, A. (2007). Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussels Mytilus galloprovincialis for assessing heavy metals pollution in coastal areas from the Saronikos Gulf of Greece. Marine Pollution Bulletin, 54, 1361-1371. DOI:10.1016/j.marpolbul.2007.05.018
  • Yalçın, M. (2006). Effect of environmental factors on growth, biochemical parameters and condition index in freshwater mussel (Unio pictorum Linnaeus, 1758). (Master Thesis) Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, Çanakkale, 59s, (only English abstract).

Details

Primary Language English
Subjects Ecology
Journal Section Articles
Authors

Ayşe ŞAHİN 0000-0002-6609-2687

Mustafa KARATEPE 0000-0001-6358-5913

Publication Date June 15, 2022
Submission Date September 27, 2021
Published in Issue Year 2022Volume: 39 Issue: 2

Cite

APA ŞAHİN, A., & KARATEPE, M. (2022). Vitamins A, E, C, β -carotene contents and MDA level of freshwater mussel, (Unio elongatulus eucirrus Bourguignat 1860) in the Karakaya Dam Lake. Ege Journal of Fisheries and Aquatic Sciences, 39(2), 120-124. https://doi.org/10.12714/egejfas.39.2.05