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1771) (Amphibia: Anura) specimens collected around Vize (Kırklareli) and Ida mountains (Çanakkale, Turkey).

Year 2012, Volume: 29 Issue: 3, 133 - 136, 01.09.2012

Mert Gürkan Sibel Hayretdağ Batuhan Yaman Yakın Cemal Varol Tok

Abstract

Keywords

-

References

  • Ahmad, K., Saleh, J. 2010. Clastogenic studies on Tandaha Dam water in Asser. Journal of Black Sea/Mediterranean Environment, 16 (1): 33- 42.
  • Anonymous, 2008. Meriç-Ergene Havzası Koruma Eylem Planı. T.C. Çevre ve Orman Bakanlığı. 1-205.
  • Aymak, C. 2010. Determination of heavy metal pollution and its genotoxic effects by using micronucleus test in Rana ridibunda Pallas, 1771 (Ranidae, Amphibia) living in Mersin. Mersin University, Phd Thesis. (in Turkish)
  • Bolognesi, C., Hayashi, M. 2011. Micronucleus assay in aquatic animals. Mutagenesis, 26 (1): 205-213. doi: 10.1093/mutage/geq073
  • Campana, M.A., Panzeri, A.M., Moreno, V.J., Dulout, F.N. 2003. Micronuclei induction in Rana catesbeiana tadpoles by the pyrethroid insecticide lambda-cyhalothrin. Genetics and Molecular Biology, 26 (1): 99-103.
  • Carrasco, K.R., Tilbury, K.L., Myers, M.S. 1990. An assessment of piscine micronucleus test as an in-situ biological indicator of chemical contaminant effects. Canadian Journal of Fisheries and Aquatic Sciences, 47: 2723-2136. doi: 10.1139/f90-237
  • Çavaş, T., Ergene-Gözükara, S. 2005. Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents. Aquatic Toxicology, 74: 264-271. doi:10.1016/j.aquatox.2005.06.00
  • Çelikler, S., Yıldız, G., Vatan, O., Bilaloğlu, R. 2008. In vitro antigenotoxicity of Ulva rigida C. Agardh (Chlorophyceae) extract against induction of chromosome aberration, sister chromatid exchange and micronuclei by mutagenic agent MMC. Biomedical and Environmental Sciences, 21: 492-498. doi: 10.1016/S0895-3988(09)60008-8
  • Erkmen, B., Kolankaya, D. 2005. Determination of organochlorine pesticide residues in water, sediment and fish samples from the Meriç Delta, Turkey, International Journal of Environmental and Analytical Chemistry, 86 (1-2): 161-169. doi: 10.1080/03067310500247926
  • Feng, S., Kong, Z., Wang, X., Zhao, L., Peng, P. 2004. Acute toxicity and genotoxicity of two novel pesticides on amphibian, Rana N. Hallowell. Chemosphere, 56 (5): 457-463. doi: 10.1016/j.chemosphere.2004.02.010
  • Finizio, A., Calliera, M., Vighi, M. 2001. Rating systems for pesticide risk classification on different ecosystems. Ecotoxicology and Environmental Safety, 49: 262-274. doi: 10.1006/eesa.2001.2063
  • Kryukov, V.I. 2000. Effects of lead on the induction of micronuclei in anuran larvae. Russian Journal of Ecology, 31 (6): 442- 443. doi: 10.1023/A:1026604212612
  • Lajmanovich, R.C., Cabagna, M., Peltzer, P.M., Stringhini, G.A., Attademo, A.M. 2005. Micronucleus induction in erythrocytes of Hyla pulchella tadpoles (Amphibia: Hylidae) exposed to insecticide endosulfan. Mutation Research, 587: 67-72. doi: 10.1016/j.mrgentox.2005.08.001
  • Lewis, S.M., Bain, B.J., Bates, I. 2006. Dacie and Lewis practical hematology. Tenth Edition Churchill Livingstone.
  • Marques, S.M., Antunes, S.C., Pissarra, H., Pereira, M.L., Goncalves, F., Pereira, R. 2009. Histopathological changes and erythrocytic nuclear abnormalities in Iberian green frogs (Rana perezi Seoane) from uranium mine pond. Aquatic Toxicology, 91: 187-195. doi: 10.1016/j.aquatox.2008.04.010
  • Orhan, G., Ekmekci, A., Menevse, S. 1993. The effect of the male contraceptive agent gossypol acetic acid on mouse bone marrow cells in vivo: micronuclei and mitotic index. Contraception, 47: 377-385. doi: 10.1016/0010-7824(93)90035-6
  • Saleh, K., Zeytinoğlu, H. 2001. Micronucleus test in peripheral erythrocytes
  • of Rana ridibunda as an indicator of environmental pollution. Anadolu
  • University Journal of Science and Technology, 2: 77- 82.
  • Saleh, K., Sarhan, M. 2007. Clastogenic analysis of chicken farms using micronucleus test in peripheral blood. Journal of A Research, 3: 1646-1649. pplied Sciences
  • Schmid, W. 1975. The micronucleus test. Mutation Research, 31: 9-15.
  • Schuytema, G.S., Nebeker, A.V. 1999. Effects of ammonium nitrate, sodium nitrate and urea on Red-Legged frogs.- Pacific treefrogs and African clawed frogs. Bulletin of Environmental Contamination and Toxicology, 63: 357-364. doi: 10.1007/s001289900988
  • Souza, T.S., Fontanelli, C.S. 2006. Micronucleus test and observation of nuclear alterations in erythrocytes of Nile tilapia exposed to waters affected by refinery effluents. Mutation Research, 605: 87-93.
  • Sparling, D.W.,Fellers, G.M., McConnell, L.L. 2001. Pesticides and amphibian populations declines in California, USA. Environmental Toxicology and Chemistry, 20: 1591-1595.
  • Villarini, M., Moretti, M., Pasquini, R., Scassellati-Sforzolini, G., Fatigoni, C. Marcarelli, M., Monarca, S., Rodriguez, A.V. 1998. In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage ('comet' assay) in relation to the induction of sister-chromatid exchanges and micronuclei. Toxicology, 130: 129-139. doi: 10.1016/S0300-483X(98)00097-3
  • Yin, X.H., Zhu, G.N., Li, X.B, Liu, S.Y. 2009. Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 680 (1-2): 2-6. doi: 10.1016/j.mrgentox.2009.05.018
  • Zhuleva, L., Umnova, N.V., Rumak, V.S. 1996. Detection of micronuclei in desquamating cells of the human oral mucosa in the territory of South Vietnam. Genetika, 32: 1700-1704.
  • Zoll-Moreux, C., Ferrier, V. 1999. The Jaylet test (Newt Micronucleus test) and the Micronucleus test in Xenopus: two in vivo tests on amphibia evaluation of the genotoxicity of five environmental pollutants and of five effluents. Water Research, 33 (10): 2301-2314.

A preliminary study on micronucleus analysis and nuclear anomalies in Pelophylax ridibundus (Pallas,

Year 2012, Volume: 29 Issue: 3, 133 - 136, 01.09.2012

Mert Gürkan Sibel Hayretdağ Batuhan Yaman Yakın Cemal Varol Tok

Abstract

Bu çalışmada Vize (Kırklareli) ve Kazdağı (Yenice, Çanakkale) civarında toplanan Pelophylax ridibundus örneklerine ait eritrositlerde mikronukleus analizi yapıldı ve nuklear anomaliler tespit edildi. Bu amaçla Nisan 2011’de Vize’den 9 (5♂♂, 4♀♀), Mayıs 2011’de Kazdağı’ndan 5 (3♂♂, 2♀♀) adet P. ridibundus örneği toplandı. Vize’den toplanan örneklerde ortalama mikronukleus sayısı 6±4,17, frekans % 0,3; Kazdağı’ndan toplanan örneklerde ise 0,6±0,43, frekans % 0,03 olarak hesaplandı. İstatistiksel analizler neticesinde iki lokalite arasında toplam mikronukleus sayısı bakımından önemli fark bulunduğu belirlendi (p≤0,05). Çalışmada binuklear, çentikli, tomurcuklu ve küçük loplu olmak üzere 4 tip nuklear anomali saptandı. Vize ve Kazdağı örnekleri arasında toplam nuklear anomali sayısı bakımından fark tespit edilmedi (p=0,31). Araştırmamız sonunda elde edilen bulgulara göre, Vize örneklerinde mikronukleus frekansının daha yüksek bulunması, bölgedeki yoğun tarımsal faaliyetlerde kullanılan genotoksik pestisitlere maruz kalınmayla ilişkilendirilebilir

Keywords

Pelophylax ridibundus Mikronukleus Nuklear anomaliler Vize Kaz Dağları

References

  • Ahmad, K., Saleh, J. 2010. Clastogenic studies on Tandaha Dam water in Asser. Journal of Black Sea/Mediterranean Environment, 16 (1): 33- 42.
  • Anonymous, 2008. Meriç-Ergene Havzası Koruma Eylem Planı. T.C. Çevre ve Orman Bakanlığı. 1-205.
  • Aymak, C. 2010. Determination of heavy metal pollution and its genotoxic effects by using micronucleus test in Rana ridibunda Pallas, 1771 (Ranidae, Amphibia) living in Mersin. Mersin University, Phd Thesis. (in Turkish)
  • Bolognesi, C., Hayashi, M. 2011. Micronucleus assay in aquatic animals. Mutagenesis, 26 (1): 205-213. doi: 10.1093/mutage/geq073
  • Campana, M.A., Panzeri, A.M., Moreno, V.J., Dulout, F.N. 2003. Micronuclei induction in Rana catesbeiana tadpoles by the pyrethroid insecticide lambda-cyhalothrin. Genetics and Molecular Biology, 26 (1): 99-103.
  • Carrasco, K.R., Tilbury, K.L., Myers, M.S. 1990. An assessment of piscine micronucleus test as an in-situ biological indicator of chemical contaminant effects. Canadian Journal of Fisheries and Aquatic Sciences, 47: 2723-2136. doi: 10.1139/f90-237
  • Çavaş, T., Ergene-Gözükara, S. 2005. Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents. Aquatic Toxicology, 74: 264-271. doi:10.1016/j.aquatox.2005.06.00
  • Çelikler, S., Yıldız, G., Vatan, O., Bilaloğlu, R. 2008. In vitro antigenotoxicity of Ulva rigida C. Agardh (Chlorophyceae) extract against induction of chromosome aberration, sister chromatid exchange and micronuclei by mutagenic agent MMC. Biomedical and Environmental Sciences, 21: 492-498. doi: 10.1016/S0895-3988(09)60008-8
  • Erkmen, B., Kolankaya, D. 2005. Determination of organochlorine pesticide residues in water, sediment and fish samples from the Meriç Delta, Turkey, International Journal of Environmental and Analytical Chemistry, 86 (1-2): 161-169. doi: 10.1080/03067310500247926
  • Feng, S., Kong, Z., Wang, X., Zhao, L., Peng, P. 2004. Acute toxicity and genotoxicity of two novel pesticides on amphibian, Rana N. Hallowell. Chemosphere, 56 (5): 457-463. doi: 10.1016/j.chemosphere.2004.02.010
  • Finizio, A., Calliera, M., Vighi, M. 2001. Rating systems for pesticide risk classification on different ecosystems. Ecotoxicology and Environmental Safety, 49: 262-274. doi: 10.1006/eesa.2001.2063
  • Kryukov, V.I. 2000. Effects of lead on the induction of micronuclei in anuran larvae. Russian Journal of Ecology, 31 (6): 442- 443. doi: 10.1023/A:1026604212612
  • Lajmanovich, R.C., Cabagna, M., Peltzer, P.M., Stringhini, G.A., Attademo, A.M. 2005. Micronucleus induction in erythrocytes of Hyla pulchella tadpoles (Amphibia: Hylidae) exposed to insecticide endosulfan. Mutation Research, 587: 67-72. doi: 10.1016/j.mrgentox.2005.08.001
  • Lewis, S.M., Bain, B.J., Bates, I. 2006. Dacie and Lewis practical hematology. Tenth Edition Churchill Livingstone.
  • Marques, S.M., Antunes, S.C., Pissarra, H., Pereira, M.L., Goncalves, F., Pereira, R. 2009. Histopathological changes and erythrocytic nuclear abnormalities in Iberian green frogs (Rana perezi Seoane) from uranium mine pond. Aquatic Toxicology, 91: 187-195. doi: 10.1016/j.aquatox.2008.04.010
  • Orhan, G., Ekmekci, A., Menevse, S. 1993. The effect of the male contraceptive agent gossypol acetic acid on mouse bone marrow cells in vivo: micronuclei and mitotic index. Contraception, 47: 377-385. doi: 10.1016/0010-7824(93)90035-6
  • Saleh, K., Zeytinoğlu, H. 2001. Micronucleus test in peripheral erythrocytes
  • of Rana ridibunda as an indicator of environmental pollution. Anadolu
  • University Journal of Science and Technology, 2: 77- 82.
  • Saleh, K., Sarhan, M. 2007. Clastogenic analysis of chicken farms using micronucleus test in peripheral blood. Journal of A Research, 3: 1646-1649. pplied Sciences
  • Schmid, W. 1975. The micronucleus test. Mutation Research, 31: 9-15.
  • Schuytema, G.S., Nebeker, A.V. 1999. Effects of ammonium nitrate, sodium nitrate and urea on Red-Legged frogs.- Pacific treefrogs and African clawed frogs. Bulletin of Environmental Contamination and Toxicology, 63: 357-364. doi: 10.1007/s001289900988
  • Souza, T.S., Fontanelli, C.S. 2006. Micronucleus test and observation of nuclear alterations in erythrocytes of Nile tilapia exposed to waters affected by refinery effluents. Mutation Research, 605: 87-93.
  • Sparling, D.W.,Fellers, G.M., McConnell, L.L. 2001. Pesticides and amphibian populations declines in California, USA. Environmental Toxicology and Chemistry, 20: 1591-1595.
  • Villarini, M., Moretti, M., Pasquini, R., Scassellati-Sforzolini, G., Fatigoni, C. Marcarelli, M., Monarca, S., Rodriguez, A.V. 1998. In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage ('comet' assay) in relation to the induction of sister-chromatid exchanges and micronuclei. Toxicology, 130: 129-139. doi: 10.1016/S0300-483X(98)00097-3
  • Yin, X.H., Zhu, G.N., Li, X.B, Liu, S.Y. 2009. Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 680 (1-2): 2-6. doi: 10.1016/j.mrgentox.2009.05.018
  • Zhuleva, L., Umnova, N.V., Rumak, V.S. 1996. Detection of micronuclei in desquamating cells of the human oral mucosa in the territory of South Vietnam. Genetika, 32: 1700-1704.
  • Zoll-Moreux, C., Ferrier, V. 1999. The Jaylet test (Newt Micronucleus test) and the Micronucleus test in Xenopus: two in vivo tests on amphibia evaluation of the genotoxicity of five environmental pollutants and of five effluents. Water Research, 33 (10): 2301-2314.
There are 28 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Mert Gürkan

Sibel Hayretdağ

Batuhan Yaman Yakın

Cemal Varol Tok

Publication Date September 1, 2012
Submission Date December 7, 2015
Published in Issue Year 2012Volume: 29 Issue: 3

Cite

APA Gürkan, M. ., Hayretdağ, S. ., Yakın, B. Y. ., Tok, C. V. . (2012). A preliminary study on micronucleus analysis and nuclear anomalies in Pelophylax ridibundus (Pallas,. Ege Journal of Fisheries and Aquatic Sciences, 29(3), 133-136.