Öz
Selenyum, tüm canlı organizmalar için gerekli bir elementtir. Eksikliği sağlık sorunlarına neden olabileceğinden beslenme sırasında dışarıdan besinler veya takviyeler yoluyla sağlanır. Mikroalgler, besin zincirindeki nihai tüketici insana taşınmaları sırasında suda yaşayan organizmalar arasında vektör organizmalar haline gelmiştir. Ancak selenyum yeterli oranlarda tüketilmesi gereken önemli bir takviyedir ve yüksek seviyelerde tüketilmesi toksik etki yaratacağından sağlanacak dozlar yaş, cinsiyet gibi faktörler göz önünde bulundurularak tüketilmelidir. Üst selenyum konsantrasyonu mikroalg kültürü için toksiktir. Bu nedenle Scenedesmus quadricauda kültür ortamına 50, 75, 100 ve 250 mg/L konsantrasyonlarda selenometiyonin eklenmesinin mikroalg büyümesi ve klorofil-a içeriği üzerindeki etkisi araştırılmıştır. Elde edilen sonuçlara göre kültürde en yüksek klorofil-a ve hücre sayısı 50 mg/L selenometiyonin kullanılarak 0.593±0,1µg/L ve 64±4,9 x106 hücre/mL olarak ölçülmüştür. Yüksek selenometiyonin konsantrasyonlarının Scenedesmus quadricauda kültürü için toksik olduğu tespit edilmiştir.
Anahtar Kelimeler
Kaynakça
- Chen T.F., Zheng W.J., Wong Y.S. & Yang F. (2008). Selenium-induced changes in activities of antioxidant enzymes and content of photosynthetic pigments in Spirulina platensis. Journal of Integrative Plant Biology 2008, 50 (1): 40–48
- Constantinescu-Aruxandei, D., Vlaicu, A., Marinaș, I.C., Vintila, A., C., N., Oancea, L., D., F. (2019). Effect of betaine and selenium on the growth and photosynthetic pigment production in Dunaliella salina as biostimulants. FEMS Microbiology Letters, 366, 2019, fnz257. doi: 10.1093/femsle/fnz257
- Demircan, D. (2022). Scenedesmus sp. Enrichment of the Nutrient Environment and Evaluation of the Food Compatibility of the Obtained Biomass. Aquaculture Faculty Master Thesis, Ege University,2022;92p.
- Gojkovic Z., Garbayo I., Ariza J.L.G., Marova I. & Vilchez C. (2015). Selenium bioaccumulation and toxicity in cultures of green microalgae. Algal Research 7 (2015) 106–116. http://dx.doi.org/10.1016/j.algal.2014.12.008
- Gojkovic Z., Vilchez C., Torronteras R., Vigara J., Gómez-Jacinto V., Janzer N., Gómez-Ariza J.L., Marova & I., Garbayo I. (2014). Effect of selenate on viability and selenomethionine accumulation of Chlorella sorokiniana grown in batch culture. Hindawi Publishing Corporation the Scientific World Journal, Volume 2014, Article ID 401265, 13 pages. http://dx.doi.org/10.1155/2014/401265
- Kangalgil M. & Yardımcı H. (2017). Effects of selenium on human health and its relationship with diabetes mellitus. Bozok Medical Journal, 2017;7(4):66-71
- Pronina N.A., Kovshova Y.I., Popova V.V., Lapin A.B., Alekseeva S.G., Baum R.F., Mishina I.M. & Tsoglin L.N. (2002). The Effect of Selenite Ions on Growth and Selenium Accumulation in Spirulina platensis. Russian Journal of Plant Physiology, Vol. 49, No. 2, 2002, pp. 235–241.
- Qian F., Misra S. & Prabhu K.S. (2019). Selenium and selenoproteins in prostanoid metabolism and immunity. Critical Reviews in Biochemistry and Molecular Biology,2019, Vol. 54, No. 6, 484–516. https://doi.org/10.1080/10409238.2020.1717430.
- Reunova Y.A., Aizdaicher N.A., Khristoforova N.K. & Reunov A.A. (2007). Effects of Selenium on Growth and Ultrastructure of the Marine Unicellular Alga Dunaliella salina (Chlorophyta). ISSN 1063-0740, Russian Journal of Marine Biology, 2007, Vol. 33, No. 2, pp. 125–132. © Pleiades Publishing, Ltd., 2007.
- Sánchez, M. D., Mantell, C., Rodrıguez, M., de La Ossa, E. M., Lubián, L. M., & Montero, O. (2005). Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana. Journal of Food Engineering, 66(2), 245- 251
- Sun X., Zhong Y., Huang Z., Yang Y. (2014). Selenium accumulation in unicellular green alga Chlorella vulgaris and its effects on antioxidant enzymes and content of photosynthetic pigments. PLoS One,2014;9(11): e112279.doi: 10.1371/journal.pone.0112270
- Tamára, F., Santos, C. (2015). Incorpation of selenium on microalgae as supplement to artemia and zebrafish. Aquaculture and Fisheries Master Thesis, Unıversidade do Algarve,2015;88p.
- Williams, M. J., R. S. Ogle, a. W. Knight, and R. G. Burau. 1994. “Effects of sulfate on selenate uptake and toxicity in the green alga Selenastrum Capricornutum.” Archives of Environmental Contamination and Toxicology 27(4):449–53.
- Zheng Y., Li Z., Tao M., Li J. & Hu Z. (2017). Effects of selenite on green microalga Haematococcus pluvialis: Bioaccumulation of selenium and enhancement of astaxanthinproduction. Aquatic Toxicology, 183 (2017) 21–27. http://dx.doi.org/10.1016/j.aquatox.2016.12.008
- Zhong Y., Cheng J.J. (2017). Effects of selenite on unicellular green microalga Chlorella pyrenoidosa: bioaccumulation of selenium, enhancement of photosynthetic pigments, and amino acid production. J. Agric. Food Chem. 2017, 65, 10875−10883.
Öz
Selenium is an essential element for all living organisms. Since its deficiency can cause health problems, it is supplied from the outside during nutrition through foods or supplements. Microalgae have become vector organisms among aquatic organisms during their transportation to the final consumer human in the food chain. However, selenium is an important supplement that should be consumed in sufficient proportions, and since its consumption at high levels will create a toxic effect, the doses to be provided must be consumed by considering factors such as age and gender. The upper selenium concentration is toxic for microalgae culture. For this reason, the effect of adding selenomethionine at 50, 75, 100 and 250 mg/L concentrations in the culture medium of Scenedesmus quadricauda on microalgae growth and chlorophyll-a content was investigated. According to the results obtained that the highest chlorophyll-a and cell number were measured as 0.593±0,1µg/L and 64±4,9 x106cells/mL using 50 mg/L selenomethionine in the culture. It has been detected the highest selenomethionine concentrations caused toxic for Scenedesmus quadricauda culture.
Anahtar Kelimeler
Kaynakça
- Chen T.F., Zheng W.J., Wong Y.S. & Yang F. (2008). Selenium-induced changes in activities of antioxidant enzymes and content of photosynthetic pigments in Spirulina platensis. Journal of Integrative Plant Biology 2008, 50 (1): 40–48
- Constantinescu-Aruxandei, D., Vlaicu, A., Marinaș, I.C., Vintila, A., C., N., Oancea, L., D., F. (2019). Effect of betaine and selenium on the growth and photosynthetic pigment production in Dunaliella salina as biostimulants. FEMS Microbiology Letters, 366, 2019, fnz257. doi: 10.1093/femsle/fnz257
- Demircan, D. (2022). Scenedesmus sp. Enrichment of the Nutrient Environment and Evaluation of the Food Compatibility of the Obtained Biomass. Aquaculture Faculty Master Thesis, Ege University,2022;92p.
- Gojkovic Z., Garbayo I., Ariza J.L.G., Marova I. & Vilchez C. (2015). Selenium bioaccumulation and toxicity in cultures of green microalgae. Algal Research 7 (2015) 106–116. http://dx.doi.org/10.1016/j.algal.2014.12.008
- Gojkovic Z., Vilchez C., Torronteras R., Vigara J., Gómez-Jacinto V., Janzer N., Gómez-Ariza J.L., Marova & I., Garbayo I. (2014). Effect of selenate on viability and selenomethionine accumulation of Chlorella sorokiniana grown in batch culture. Hindawi Publishing Corporation the Scientific World Journal, Volume 2014, Article ID 401265, 13 pages. http://dx.doi.org/10.1155/2014/401265
- Kangalgil M. & Yardımcı H. (2017). Effects of selenium on human health and its relationship with diabetes mellitus. Bozok Medical Journal, 2017;7(4):66-71
- Pronina N.A., Kovshova Y.I., Popova V.V., Lapin A.B., Alekseeva S.G., Baum R.F., Mishina I.M. & Tsoglin L.N. (2002). The Effect of Selenite Ions on Growth and Selenium Accumulation in Spirulina platensis. Russian Journal of Plant Physiology, Vol. 49, No. 2, 2002, pp. 235–241.
- Qian F., Misra S. & Prabhu K.S. (2019). Selenium and selenoproteins in prostanoid metabolism and immunity. Critical Reviews in Biochemistry and Molecular Biology,2019, Vol. 54, No. 6, 484–516. https://doi.org/10.1080/10409238.2020.1717430.
- Reunova Y.A., Aizdaicher N.A., Khristoforova N.K. & Reunov A.A. (2007). Effects of Selenium on Growth and Ultrastructure of the Marine Unicellular Alga Dunaliella salina (Chlorophyta). ISSN 1063-0740, Russian Journal of Marine Biology, 2007, Vol. 33, No. 2, pp. 125–132. © Pleiades Publishing, Ltd., 2007.
- Sánchez, M. D., Mantell, C., Rodrıguez, M., de La Ossa, E. M., Lubián, L. M., & Montero, O. (2005). Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana. Journal of Food Engineering, 66(2), 245- 251
- Sun X., Zhong Y., Huang Z., Yang Y. (2014). Selenium accumulation in unicellular green alga Chlorella vulgaris and its effects on antioxidant enzymes and content of photosynthetic pigments. PLoS One,2014;9(11): e112279.doi: 10.1371/journal.pone.0112270
- Tamára, F., Santos, C. (2015). Incorpation of selenium on microalgae as supplement to artemia and zebrafish. Aquaculture and Fisheries Master Thesis, Unıversidade do Algarve,2015;88p.
- Williams, M. J., R. S. Ogle, a. W. Knight, and R. G. Burau. 1994. “Effects of sulfate on selenate uptake and toxicity in the green alga Selenastrum Capricornutum.” Archives of Environmental Contamination and Toxicology 27(4):449–53.
- Zheng Y., Li Z., Tao M., Li J. & Hu Z. (2017). Effects of selenite on green microalga Haematococcus pluvialis: Bioaccumulation of selenium and enhancement of astaxanthinproduction. Aquatic Toxicology, 183 (2017) 21–27. http://dx.doi.org/10.1016/j.aquatox.2016.12.008
- Zhong Y., Cheng J.J. (2017). Effects of selenite on unicellular green microalga Chlorella pyrenoidosa: bioaccumulation of selenium, enhancement of photosynthetic pigments, and amino acid production. J. Agric. Food Chem. 2017, 65, 10875−10883.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Hidrobiyoloji |
| Bölüm | Araştırmalar |
| Yazarlar | |
| Yayımlanma Tarihi | 27 Haziran 2023 |
| Kabul Tarihi | 13 Haziran 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 9 Sayı: 1 |
