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Evaluation of Antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTSPersulphate and DMPD methods

Yıl 2015, Cilt: 19 Sayı: 2, 153 - 158, 15.01.2015

Ayça Karasakal

https://doi.org/10.12991/mpj.20151910462

Öz

The antioxidant activities of the extracts prepared from Brassica napus’s seeds (canola seeds) was determined in this study. Brassica napus’s seeds were collected from eight different region in Turkey. Extraction experiments were carried out with 100% hexane, 100% acetone, 100%  methanol, 100% ethanol, %75 hexane-water, %75 acetone-water , 75%  methanol-water, 75% ethanol-water, %50 hexane-water, 50% acetone-water, 50%  methanol-water, 50% ethanol-water at 25 °C for overnight and were mixed by magnetic stirrer during 1h, 2h, 4h, 8h  to determine optimal extraction conditions. According to the results of UV/Vis spectras, 100% hexane extraction waited at 25 °C for overnight were chosen for ABTS/Persulphate, N,N-dimethyl-p-phenylenediamine (DMPD) and CUPRAC methods. CUPRAC and ABTS/Persulphate methods’s total antioxidant capacity (TAC) were calculated as Trolox equivalent. In DMPD method, antioxidant capacity of the extracts was determined by inhibition of DMPD radical cation. DMPD method analysis results of Brassica napus’s seeds showed the greatest effectiveness, with inhibition values of 2.00 ± 0.07% and 17.1± 0.03%. The antioxidant activities of Brassica napus’s seeds ranged from 0.29±0.04 mol/g and 0.48±0.01 mol/g for CUPRAC method and 0.36 ± 0.01 mol/g and 0.55± 0.05 mol/g for ABTS/Persulphate method.

Anahtar Kelimeler

Antioxidant activity Brassica napus’s seed ABTS

Kaynakça

  • Larson RA. The antioxidants of higher plants. Phytochemistry 1988; 4 : 969–978.
  • Hertog MGL, Hollman PCH, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Nederlands. J. Agric. Food Chem 1992; 40 : 2379–2383.
  • Cao G, Sofic E, Prior RL. Antioxidant capacity of tea and common vegetables. J. Agric. Food Chem 1996; 44 :3426–3431.
  • Kivits GAA, Vam der Sman FJP, Tijburg LBM. Analysis of catechin from green and black tea in humans: a specific and sensitive colorimetric assay of total catechins in biological fluids. Int. J. Food Sci. Nutr.1997; 48 : 387–392.
  • Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology 2000; 32 :81–118.
  • Exarchou V, Nenadis N, Tsimidou M, Gerothanassis IP, Troganis A, Boskou D. Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage and summer savory. Journal of Agricultural and Food Chemistry 2002; 50 : 5294–5299.
  • Hirasa K, Takemasa M. Spice science and technology, Marcel Dekker, New York . 1998.
  • Riaz T, Abbasi MA, Aziz-ur-Rehman, Shahzadi T, Qureshi MZ, Ajaib M. Dicliptera bupleuroides: an imperative Source for Protection from Oxidative Stress. J. Chem. Soc. Pak. 2012; 34 : 326-331.
  • Paravicini TM, Touyz RM. NADPH oxidase, reactive oxygen species, and hypertension. Diabetes care 2008; 31: 170-180.
  • Çekiç SD, Çetinkaya A, Avan AN, Apak R. Correlation of Total Antioxidant Capacity with Reactive Oxygen Species (ROS) Consumption Measured by Oxidative Conversion. J. Agric. Food Chem. 2013; 61: 5260−5270.
  • Perez-Jimenez J, Saura-Calixto F. Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Res. Int. 2006; 39: 791-800.
  • Finotti E, Majo DD. Influence of solvents on the antioxidant property of flavonoids. Nahrung/Food.2003; 47:186-187.
  • Basolo F, Pearson RG. Mechanism of Inorganic Reactions—A Study of Metal Complexes in Solution, second ed., John Wiley & Sons, New York, 1960, pp. 303–331.
  • Baskan KS, Tutem E, Ozer N. Spectrophotometric and Chromatographic Assessment of Contributions of Carotenoids and Chlorophylls to the Total Antioxidant Capacities of Plant Foods.Journal of Agricultural and Food Chemistry 2013; 61: 11371-11381.
  • Celik ES, Ozyurek M, Guclu K. Solvent effects on the antioxidant capacity of lipophilic and hydrophilic antioxidants measured by CUPRAC, ABTS/persulfate and FRAP methods.Talanta 2010; 81: 1300-1309.
  • Khatoon M, Islam E, Islam R, Rahman AA, Alam AHM K, Khondkar P, Rashid M, Parvin S. Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Research Notes. 2013; 6:121-128.
  • Karaman Ş, Tütem E, Başkan KS, Apak R. Comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC–CUPRAC assay. Food Chemistry. 2010; 120: 1201-1209.
  • Vincenzo F, Verde V, Giacomino R, Ritieni A. Method for Measuring Antioxidant Activity and Its Application to Monitoring the Antioxidant Capacity of Wines. J.Agric.Food Chem. 1999; 47:1035-1039.
  • Karaman Ş, Tütem E, Başkan KS, Apak R. Comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC–CUPRAC assay. Food Chemistry 2010; 120: 1201-1209.
  • Fogliano V, Verde V, Randazzo G, Ritieni A. Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J. Agric. Food Chem. 1999; 47 : 1035–1040.
  • Amarowicz R, Naczk M, Shahidi F. Antioxidant activity of crude tannins of canola and rapeseed hulls. Journal of the American Oil Chemists' Society.2000;77:957-961.
  • Cumby N, Zhong Y, Naczk M, Fereidoon S. Antioxidant activity and water-holding capacity of canola protein hydrolysates. Food Chemistry.2008;109:144-148.
  • Amarowicz R, Naczk M, Zadernowski R, Shahidi F. Antioxidant activity of condensed tannins of beach pea, canola hulls, evening primrose, and faba bean.Journal of Food Lipids.2000;7:195-205.
  • Wikipedia. Canola. Health information. Erişim tarihi:28.11.2014

Yıl 2015, Cilt: 19 Sayı: 2, 153 - 158, 15.01.2015

Ayça Karasakal

https://doi.org/10.12991/mpj.20151910462

Öz

Kaynakça

  • Larson RA. The antioxidants of higher plants. Phytochemistry 1988; 4 : 969–978.
  • Hertog MGL, Hollman PCH, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Nederlands. J. Agric. Food Chem 1992; 40 : 2379–2383.
  • Cao G, Sofic E, Prior RL. Antioxidant capacity of tea and common vegetables. J. Agric. Food Chem 1996; 44 :3426–3431.
  • Kivits GAA, Vam der Sman FJP, Tijburg LBM. Analysis of catechin from green and black tea in humans: a specific and sensitive colorimetric assay of total catechins in biological fluids. Int. J. Food Sci. Nutr.1997; 48 : 387–392.
  • Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology 2000; 32 :81–118.
  • Exarchou V, Nenadis N, Tsimidou M, Gerothanassis IP, Troganis A, Boskou D. Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage and summer savory. Journal of Agricultural and Food Chemistry 2002; 50 : 5294–5299.
  • Hirasa K, Takemasa M. Spice science and technology, Marcel Dekker, New York . 1998.
  • Riaz T, Abbasi MA, Aziz-ur-Rehman, Shahzadi T, Qureshi MZ, Ajaib M. Dicliptera bupleuroides: an imperative Source for Protection from Oxidative Stress. J. Chem. Soc. Pak. 2012; 34 : 326-331.
  • Paravicini TM, Touyz RM. NADPH oxidase, reactive oxygen species, and hypertension. Diabetes care 2008; 31: 170-180.
  • Çekiç SD, Çetinkaya A, Avan AN, Apak R. Correlation of Total Antioxidant Capacity with Reactive Oxygen Species (ROS) Consumption Measured by Oxidative Conversion. J. Agric. Food Chem. 2013; 61: 5260−5270.
  • Perez-Jimenez J, Saura-Calixto F. Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Res. Int. 2006; 39: 791-800.
  • Finotti E, Majo DD. Influence of solvents on the antioxidant property of flavonoids. Nahrung/Food.2003; 47:186-187.
  • Basolo F, Pearson RG. Mechanism of Inorganic Reactions—A Study of Metal Complexes in Solution, second ed., John Wiley & Sons, New York, 1960, pp. 303–331.
  • Baskan KS, Tutem E, Ozer N. Spectrophotometric and Chromatographic Assessment of Contributions of Carotenoids and Chlorophylls to the Total Antioxidant Capacities of Plant Foods.Journal of Agricultural and Food Chemistry 2013; 61: 11371-11381.
  • Celik ES, Ozyurek M, Guclu K. Solvent effects on the antioxidant capacity of lipophilic and hydrophilic antioxidants measured by CUPRAC, ABTS/persulfate and FRAP methods.Talanta 2010; 81: 1300-1309.
  • Khatoon M, Islam E, Islam R, Rahman AA, Alam AHM K, Khondkar P, Rashid M, Parvin S. Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Research Notes. 2013; 6:121-128.
  • Karaman Ş, Tütem E, Başkan KS, Apak R. Comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC–CUPRAC assay. Food Chemistry. 2010; 120: 1201-1209.
  • Vincenzo F, Verde V, Giacomino R, Ritieni A. Method for Measuring Antioxidant Activity and Its Application to Monitoring the Antioxidant Capacity of Wines. J.Agric.Food Chem. 1999; 47:1035-1039.
  • Karaman Ş, Tütem E, Başkan KS, Apak R. Comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC–CUPRAC assay. Food Chemistry 2010; 120: 1201-1209.
  • Fogliano V, Verde V, Randazzo G, Ritieni A. Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J. Agric. Food Chem. 1999; 47 : 1035–1040.
  • Amarowicz R, Naczk M, Shahidi F. Antioxidant activity of crude tannins of canola and rapeseed hulls. Journal of the American Oil Chemists' Society.2000;77:957-961.
  • Cumby N, Zhong Y, Naczk M, Fereidoon S. Antioxidant activity and water-holding capacity of canola protein hydrolysates. Food Chemistry.2008;109:144-148.
  • Amarowicz R, Naczk M, Zadernowski R, Shahidi F. Antioxidant activity of condensed tannins of beach pea, canola hulls, evening primrose, and faba bean.Journal of Food Lipids.2000;7:195-205.
  • Wikipedia. Canola. Health information. Erişim tarihi:28.11.2014
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Makaleler
Yazarlar

Ayça Karasakal

Yayımlanma Tarihi 15 Ocak 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 19 Sayı: 2

Kaynak Göster

APA Karasakal, A. (2015). Evaluation of Antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTSPersulphate and DMPD methods. Marmara Pharmaceutical Journal, 19(2), 153-158. https://doi.org/10.12991/mpj.20151910462
AMA Karasakal A. Evaluation of Antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTSPersulphate and DMPD methods. mpj. Nisan 2015;19(2):153-158. doi:10.12991/mpj.20151910462
Chicago Karasakal, Ayça. “Evaluation of Antioxidant Activities of Brassica napus’s Seeds by CUPRAC, ABTSPersulphate and DMPD Methods”. Marmara Pharmaceutical Journal 19, sy. 2 (Nisan 2015): 153-58. https://doi.org/10.12991/mpj.20151910462.
EndNote Karasakal A (01 Nisan 2015) Evaluation of Antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTSPersulphate and DMPD methods. Marmara Pharmaceutical Journal 19 2 153–158.
IEEE A. Karasakal, “Evaluation of Antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTSPersulphate and DMPD methods”, mpj, c. 19, sy. 2, ss. 153–158, 2015, doi: 10.12991/mpj.20151910462.
ISNAD Karasakal, Ayça. “Evaluation of Antioxidant Activities of Brassica napus’s Seeds by CUPRAC, ABTSPersulphate and DMPD Methods”. Marmara Pharmaceutical Journal 19/2 (Nisan 2015), 153-158. https://doi.org/10.12991/mpj.20151910462.
JAMA Karasakal A. Evaluation of Antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTSPersulphate and DMPD methods. mpj. 2015;19:153–158.
MLA Karasakal, Ayça. “Evaluation of Antioxidant Activities of Brassica napus’s Seeds by CUPRAC, ABTSPersulphate and DMPD Methods”. Marmara Pharmaceutical Journal, c. 19, sy. 2, 2015, ss. 153-8, doi:10.12991/mpj.20151910462.
Vancouver Karasakal A. Evaluation of Antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTSPersulphate and DMPD methods. mpj. 2015;19(2):153-8.