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Sideritis akmanii'den Elde Edilen Sulu ve Etanol Ekstraktlarının Antioksidan ve Histopatolojik Etkilerinin Araştırılması

Yıl 2023, Cilt: 16 Sayı: 3, 383 - 392, 30.09.2023

İsmail Küçükkurt Fahriye Kan Sinan İnce Hasan Hüseyin Demirel Erten Akbel Damla Arslan Acaröz

Öz

Bu çalışmada, Afyonkarahisar ili Kumalar yaylası bölgesinde Şuhut-Sandıklı yolu üzerinde yayılış gösteren endemik Sideritis akmanii bitkisinden elde edilen sulu ve etanolik ekstraktların antioksidan aktivitelerinin ve histopatolojik etkilerinin araştırılması amaçlanmıştır. 48 adet Wistar albino erkek rat 8 gruba ayrıldı; Kontrol Grubu (Grup I), CMC Grubu (Grup II), %0,5 CMC (karboksimetil selüloz), Sulu ve etanolik bitki ekstrakt grupları (Grup III-IV-V-VI-VII-VIII). Belirlenen LD50 dozundan uygun oranlarda üç farklı doz (%1, %2 ve %4) ekstraktı 6 deney grubuna 30 gün uygulandı. Deney sonunda sağlıklı ratların kan ve doku (karaciğer, kalp, beyin, böbrek, testis) örneklerinde malondialdehit (MDA), glutatyon (GSH) düzeyleri, süperoksit dismutaz (SOD) ve katalaz (CAT) enzim aktiviteleri ölçüldü.Tam kan MDA düzeyi etanol ekstrakt grubunun tüm dozlarında kontrol grubuna göre azalırken, GSH düzeyi her iki ekstrakt grubunda da kontrol grubuna göre arttı. Etanol gruplarında eritrosit ve testis dokusu dışında SOD ve CAT enzim aktiviteleri kontrol grubuna göre arttı (p<0.05). Histopatolojik inceleme sonuçlarında ise herhangi bir değişiklik gözlenmedi. Çalışma sonucunda Sideritis akmanii'nin sulu ve etanol ekstraktlarının antioksidan özelliklere sahip olduğu ve etanol ekstraktının antioksidan enzimler üzerinde daha etkili olduğu gözlemlendi.

Anahtar Kelimeler

Antioksidan enzimler Histopatoloji Lipid peroksidasyonu Sideritis akmanii ekstraktı (sulu ve etanollü)

Proje Numarası

Project no: 18.KARIYER.190

Kaynakça

  • Aebi H. Catalase in vitro, in: U. Bergmeyer (Ed.), Methods of enzymatic analysis. Academic Press, New York and London. 1974; pp. 673-677. https://doi.org/10.1016/B978-0-12-091302-2.50032-3
  • Aksoy L, Güzey I, Düz M. Essential Oil Content, Antioxidative Characteristics and Enzyme Inhibitory Activity of Sideritis akmanii Aytaç, Ekici & Dönmez. Turkish Journal of Pharmaceutical Sciences 2022; 19(1): 76. https://doi.org/10.4274/tjps.galenos.2021.86422
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. Journal of Laboratoty and Clinical Medicine 1993; 61: 882-888.
  • Bondı̀ ML, Bruno M, Piozzi F, Baser KHC, Simmonds, MS. Diversity and antifeedant activity of diterpenes from Turkish species of Sideritis. Biochemical systematics and ecology 2000; 28(4): 299-303.
  • Celik I, Kaya MS. The antioxidant role of Sideritis caesarea infusion against TCA toxicity in rats. British journal of nutrition 2011; 105(5) 663-668. https://doi.org/10.1017/S0007114510004265
  • Cigerci IH, Turhan H, Hazman O, Isitez N. The effects of Sideritis akmanii on endoplasmic reticulum stress, inflammation, and DNA damage in experimentally ER-stress-induced MCF-7 cancer cells. International Journal of Plant Based Pharmaceuticals 2023; 3(1): 47-53. https://doi.org/10.29228/ijpbp.15
  • Coğuplugil A. The investigation of effects of extracts obtain from Sideritis akmanii on lipid profile and some biochemical parameters in rats. MSc, Afyon Kocatepe University Health Science Institute, Afyon, 2019.
  • Çarıkçı S. Antioxidant and Anticholinesterase Properties of Sideritis perfoliata subsp. athoa (Papan. &Kokkini) Baden and Sideritis trojana Bornm. Teas from Mount Ida-Turkey and Their Phenolic Characterization by LC-MS/MS. Journal of the Turkish Chemical Society Section A: Chemistry, 2020; 7(2): 617-634. https://doi.org/10.18596/jotcsa.718274
  • Çelik Y, Küçükkurt İ. Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats. Kocatepe Veterinary Journal 2016; 9(4): 259-2650. https://doi.org/10.5578/kvj.29164
  • Day BJ. Catalase and glutathione peroxidase mimics. Biochemical pharmacology 2009; 77(3): 285-296. https://doi.org/10.1016/j.bcp.2008.09.029
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. In Methods in enzymology 1990; 186: 421-431. https://doi.org/10.1016/0076-6879(90)86135-I
  • Drabkin DL, Austin JH. Spectrophotometric Studies. II. Preparations From Washed Blood Cells; Nitric Oxide Hemoglobin and Sulfhemoglobin Journal of Biological Chemistry 1935; 112: 51–65. https://doi.org/10.1016/S0021-9258(18)74965-X
  • Dündar Y, Aslan R. Hekimlikte Oksidatif Stres ve Antioksidanlar. Afyon, Turkey: Afyon Kocatepe Üniversitesi Yayınları, 2000.
  • Feistel B, Wegener T, Rzymski P, Pischel I. Assessment of the Acute and Subchronic Toxicity and Mutagenicity of Sideritis scardica Griseb. Extracts. Toxins 2018; 10(7): 258. https://doi.org/10.3390/toxins10070258
  • González-Burgos E, Carretero ME, Gómez-Serranillos MP. Sideritis spp.: Uses, chemical composition and pharmacological activities—A review. Journal of Ethnopharmacology 2011; 217-219. https://doi.org/10.1016/j.jep.2011.03.014
  • Güvenç A, Okada Y, Akkol EK, Duman H, Okuyama T, Çalış İ. Investigations of anti-inflammatory, antinociceptive, antioxidant and aldose reductase inhibitory activities of phenolic compounds from Sideritis brevibracteata. Food Chemistry 2010; 118(3): 686-692. https://doi.org/10.1016/j.foodchem.2009.05.034
  • Güzey İ. Determınatıon of Afyon endemıc plant Siderıtıs akmanii free radical scavening,the total phenolic substances, total antıoxidant and an oxidant status and mineral matter content. MSc, Afyon Kocatepe University Science Institute, Afyon, Turkey, 2017.
  • Kucukkurt I, Ince S, Fidan AF, Ozdemir A. The effects of dietary supplementation of different amount of Yucca schidigera powder (Sarsaponin 30®) on blood and tissue antioxidant defense systems and lipid peroxidation in rats. Journal of Animal and Veterinary Advances 2008; 7: 1413-1417.
  • Linardaki Z, Papandreou MA, Iatrou G, Lamari FN, Margarity M. Ethnobotanical notes about some uses of medicinal plants in Alto Tirreno Cosentino area (Calabria, Southern Italy). Journal of Ethnobiology and Ethnomedicine 2008; 3: 1-6. https://doi.org/10.1186/1746-4269-3-34
  • Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4(8): 118–126. https://doi.org/10.4103/0973-7847.70902
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry 1951; 193: 265-75. https://doi.org/10.1016/S0021-9258(19)52451-6
  • Luck H. Catalase. (Bergmeyer, H.U. (ed). Methods in analysis London: Academy Press 1955.
  • OECD – (Organisation for Economic Co-operation and Development) Guidelines for the Testing of Chemicals, Oecd 423. Acute Oral ToxicityAcute Toxic Class Method. Paris: Organisation for Economic Cooperation and Development, 2001.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3Get rights and content
  • Ozturk Y, Aydın S, Ozturk N, Başer KHC. “Effects of Extracts fromCertain Sideritis species on Swimming Performance in Mice”. Phytother. Res, 1996; 10: 70-73. https://doi.org/10.1002/(SICI)1099-1573(199602)10:1<70::AID-PTR785>3.0.CO;2-%23
  • Ozkan G. Comparison of antioxidant phenolics of ethanolic extracts and aqueous infusions from Sideritis species. Asian Journal of Chemistry 2009; 21(2): 1024-1028.
  • Özden TA, Ömer B, Saner G, Saner H, Müslümanoğlu M, Tuzlalı S. MDA, ZN and GSH Levels in Breast Cancer. Türk Klinik Biyokimya Dergisi 2004; 2(2): 59-64.
  • Sagdic O, Aksoy A, Ozkan G, Ekici L, Albayrak S. Biological activities of the extracts of two endemic Sideritis species in Turkey. Innovative Food Science and Emerging Technologies 2008; 9: 80–84. https://doi.org/10.1016/j.ifset.2007.06.001
  • Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry 1988; 34: 497-500. ISSN: 0009-9147
  • Surai PF, Kochish II, Fisinin VI, Kidd MT. Antioxidant defence systems and oxidative stress in poultry biology: An update. Antioxidants 2019; 8(7): 235. https://doi.org/10.3390/antiox8070235
  • Şerbetçi H. Determınation of antioxidant capacity of licorice (Glycyrrhiza glabra L.). MSc, . Atatürk University, Erzurum, Turkey, 2007.
  • Tadić VM, Jeremic I, Dobric S, Isakovic A, Markovic I, et al. Anti-inflammatory, gastroprotective, and cytotoxic effects of Sideritis scardica extracts. Planta medica 2012; 78(05): 415-427. https://doi.org/10.1055/s-0031-1298172
  • Temel M, Kara R, Müdüroğlu R, Akkaya L. Research Paper Healthcare Antibacterial Activity of Turkish Endemic Sideritis Akmanii (Lamiaceae). Healthcare 2014; 3: 4.
  • Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine 1975; 85(2): 337-341. https://doi.org/10.5555/uri:pii:0022214375904394
  • Zengin G, Sarikurkcu C, Aktumsek A, Ceylan R. Sideritis galatica Bornm.: a source of multifunctional agents for the management of oxidative damage, Alzheimer's's and diabetes mellitus. journal of functional foods, 2014; 11: 538-547. http://dx.doi.org/10.1016/j.jff.2014.08.011).

Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii

Yıl 2023, Cilt: 16 Sayı: 3, 383 - 392, 30.09.2023

İsmail Küçükkurt Fahriye Kan Sinan İnce Hasan Hüseyin Demirel Erten Akbel Damla Arslan Acaröz

Öz

In this study, it was aimed to investigate the antioxidant activities and histopathological effects of the aqueous and ethanolic extracts obtained from the endemic Sideritis akmanii plant, which spreads on the Şuhut-Sandıklı road in the Kumalar plateau region of Afyonkarahisar province. The number of 48 Wistar albino male rats were divided into 8 groups; Control Group (Group I), CMC Group (Group II), 0.5% CMC (carboxymethyl cellulose), Aqueous and ethanolic plant extract groups (Group III-IV-V-VI-VII-VIII). Three different doses (1%, 2%, and 4%) extracts from the determined LD50 dose were applied to 6 experimental groups for 30 days. At the end of the experiment, malondialdehyde (MDA), glutathione (GSH) levels, superoxide dismutase (SOD), and catalase (CAT) enzyme activities were measured in healthy rat's blood and tissue (liver, heart, brain, kidney, testis) samples. The whole blood MDA level decreased in all doses of the ethanolic extract group compared to the control group, while the GSH level increased in both extract groups compared to the control group. SOD and CAT enzyme activities were increased in the ethanolic groups compared to the control group, except for erythrocyte and testis tissue (p<0.05). On the other hand, no changes were observed in the histopathological examination results. As a result of the study, it was observed that the aqueous and ethanolic extracts of Sideritis akmanii had antioxidant properties and the ethanolic extract was more effective on antioxidant enzymes.

Anahtar Kelimeler

Antioxidant enzymes Histopathology Lipid peroxidation Sideritis akmanii extract (aqueous and ethanolic)

Destekleyen Kurum

This study was supported by Afyon Kocatepe University Scientific Research Council, Afyonkarahisar, Turkey. All authors express their gratitude to this institution.

Proje Numarası

Project no: 18.KARIYER.190

Teşekkür

We thank Prof Dr. Mustafa KARGIOGLU for identifying the Sideritis akmanii used in the study

Kaynakça

  • Aebi H. Catalase in vitro, in: U. Bergmeyer (Ed.), Methods of enzymatic analysis. Academic Press, New York and London. 1974; pp. 673-677. https://doi.org/10.1016/B978-0-12-091302-2.50032-3
  • Aksoy L, Güzey I, Düz M. Essential Oil Content, Antioxidative Characteristics and Enzyme Inhibitory Activity of Sideritis akmanii Aytaç, Ekici & Dönmez. Turkish Journal of Pharmaceutical Sciences 2022; 19(1): 76. https://doi.org/10.4274/tjps.galenos.2021.86422
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. Journal of Laboratoty and Clinical Medicine 1993; 61: 882-888.
  • Bondı̀ ML, Bruno M, Piozzi F, Baser KHC, Simmonds, MS. Diversity and antifeedant activity of diterpenes from Turkish species of Sideritis. Biochemical systematics and ecology 2000; 28(4): 299-303.
  • Celik I, Kaya MS. The antioxidant role of Sideritis caesarea infusion against TCA toxicity in rats. British journal of nutrition 2011; 105(5) 663-668. https://doi.org/10.1017/S0007114510004265
  • Cigerci IH, Turhan H, Hazman O, Isitez N. The effects of Sideritis akmanii on endoplasmic reticulum stress, inflammation, and DNA damage in experimentally ER-stress-induced MCF-7 cancer cells. International Journal of Plant Based Pharmaceuticals 2023; 3(1): 47-53. https://doi.org/10.29228/ijpbp.15
  • Coğuplugil A. The investigation of effects of extracts obtain from Sideritis akmanii on lipid profile and some biochemical parameters in rats. MSc, Afyon Kocatepe University Health Science Institute, Afyon, 2019.
  • Çarıkçı S. Antioxidant and Anticholinesterase Properties of Sideritis perfoliata subsp. athoa (Papan. &Kokkini) Baden and Sideritis trojana Bornm. Teas from Mount Ida-Turkey and Their Phenolic Characterization by LC-MS/MS. Journal of the Turkish Chemical Society Section A: Chemistry, 2020; 7(2): 617-634. https://doi.org/10.18596/jotcsa.718274
  • Çelik Y, Küçükkurt İ. Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats. Kocatepe Veterinary Journal 2016; 9(4): 259-2650. https://doi.org/10.5578/kvj.29164
  • Day BJ. Catalase and glutathione peroxidase mimics. Biochemical pharmacology 2009; 77(3): 285-296. https://doi.org/10.1016/j.bcp.2008.09.029
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. In Methods in enzymology 1990; 186: 421-431. https://doi.org/10.1016/0076-6879(90)86135-I
  • Drabkin DL, Austin JH. Spectrophotometric Studies. II. Preparations From Washed Blood Cells; Nitric Oxide Hemoglobin and Sulfhemoglobin Journal of Biological Chemistry 1935; 112: 51–65. https://doi.org/10.1016/S0021-9258(18)74965-X
  • Dündar Y, Aslan R. Hekimlikte Oksidatif Stres ve Antioksidanlar. Afyon, Turkey: Afyon Kocatepe Üniversitesi Yayınları, 2000.
  • Feistel B, Wegener T, Rzymski P, Pischel I. Assessment of the Acute and Subchronic Toxicity and Mutagenicity of Sideritis scardica Griseb. Extracts. Toxins 2018; 10(7): 258. https://doi.org/10.3390/toxins10070258
  • González-Burgos E, Carretero ME, Gómez-Serranillos MP. Sideritis spp.: Uses, chemical composition and pharmacological activities—A review. Journal of Ethnopharmacology 2011; 217-219. https://doi.org/10.1016/j.jep.2011.03.014
  • Güvenç A, Okada Y, Akkol EK, Duman H, Okuyama T, Çalış İ. Investigations of anti-inflammatory, antinociceptive, antioxidant and aldose reductase inhibitory activities of phenolic compounds from Sideritis brevibracteata. Food Chemistry 2010; 118(3): 686-692. https://doi.org/10.1016/j.foodchem.2009.05.034
  • Güzey İ. Determınatıon of Afyon endemıc plant Siderıtıs akmanii free radical scavening,the total phenolic substances, total antıoxidant and an oxidant status and mineral matter content. MSc, Afyon Kocatepe University Science Institute, Afyon, Turkey, 2017.
  • Kucukkurt I, Ince S, Fidan AF, Ozdemir A. The effects of dietary supplementation of different amount of Yucca schidigera powder (Sarsaponin 30®) on blood and tissue antioxidant defense systems and lipid peroxidation in rats. Journal of Animal and Veterinary Advances 2008; 7: 1413-1417.
  • Linardaki Z, Papandreou MA, Iatrou G, Lamari FN, Margarity M. Ethnobotanical notes about some uses of medicinal plants in Alto Tirreno Cosentino area (Calabria, Southern Italy). Journal of Ethnobiology and Ethnomedicine 2008; 3: 1-6. https://doi.org/10.1186/1746-4269-3-34
  • Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4(8): 118–126. https://doi.org/10.4103/0973-7847.70902
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry 1951; 193: 265-75. https://doi.org/10.1016/S0021-9258(19)52451-6
  • Luck H. Catalase. (Bergmeyer, H.U. (ed). Methods in analysis London: Academy Press 1955.
  • OECD – (Organisation for Economic Co-operation and Development) Guidelines for the Testing of Chemicals, Oecd 423. Acute Oral ToxicityAcute Toxic Class Method. Paris: Organisation for Economic Cooperation and Development, 2001.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3Get rights and content
  • Ozturk Y, Aydın S, Ozturk N, Başer KHC. “Effects of Extracts fromCertain Sideritis species on Swimming Performance in Mice”. Phytother. Res, 1996; 10: 70-73. https://doi.org/10.1002/(SICI)1099-1573(199602)10:1<70::AID-PTR785>3.0.CO;2-%23
  • Ozkan G. Comparison of antioxidant phenolics of ethanolic extracts and aqueous infusions from Sideritis species. Asian Journal of Chemistry 2009; 21(2): 1024-1028.
  • Özden TA, Ömer B, Saner G, Saner H, Müslümanoğlu M, Tuzlalı S. MDA, ZN and GSH Levels in Breast Cancer. Türk Klinik Biyokimya Dergisi 2004; 2(2): 59-64.
  • Sagdic O, Aksoy A, Ozkan G, Ekici L, Albayrak S. Biological activities of the extracts of two endemic Sideritis species in Turkey. Innovative Food Science and Emerging Technologies 2008; 9: 80–84. https://doi.org/10.1016/j.ifset.2007.06.001
  • Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry 1988; 34: 497-500. ISSN: 0009-9147
  • Surai PF, Kochish II, Fisinin VI, Kidd MT. Antioxidant defence systems and oxidative stress in poultry biology: An update. Antioxidants 2019; 8(7): 235. https://doi.org/10.3390/antiox8070235
  • Şerbetçi H. Determınation of antioxidant capacity of licorice (Glycyrrhiza glabra L.). MSc, . Atatürk University, Erzurum, Turkey, 2007.
  • Tadić VM, Jeremic I, Dobric S, Isakovic A, Markovic I, et al. Anti-inflammatory, gastroprotective, and cytotoxic effects of Sideritis scardica extracts. Planta medica 2012; 78(05): 415-427. https://doi.org/10.1055/s-0031-1298172
  • Temel M, Kara R, Müdüroğlu R, Akkaya L. Research Paper Healthcare Antibacterial Activity of Turkish Endemic Sideritis Akmanii (Lamiaceae). Healthcare 2014; 3: 4.
  • Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine 1975; 85(2): 337-341. https://doi.org/10.5555/uri:pii:0022214375904394
  • Zengin G, Sarikurkcu C, Aktumsek A, Ceylan R. Sideritis galatica Bornm.: a source of multifunctional agents for the management of oxidative damage, Alzheimer's's and diabetes mellitus. journal of functional foods, 2014; 11: 538-547. http://dx.doi.org/10.1016/j.jff.2014.08.011).
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Bilimleri
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

İsmail Küçükkurt 0000-0003-0198-629X

Fahriye Kan 0000-0002-6366-8396

Sinan İnce 0000-0002-1915-9797

Hasan Hüseyin Demirel 0000-0002-4795-2266

Erten Akbel 0000-0002-6954-3658

Damla Arslan Acaröz 0000-0001-9230-6725

Proje Numarası Project no: 18.KARIYER.190
Yayımlanma Tarihi 30 Eylül 2023
Kabul Tarihi 31 Ağustos 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 16 Sayı: 3

Kaynak Göster

APA Küçükkurt, İ., Kan, F., İnce, S., Demirel, H. H., vd. (2023). Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii. Kocatepe Veterinary Journal, 16(3), 383-392.
AMA Küçükkurt İ, Kan F, İnce S, Demirel HH, Akbel E, Arslan Acaröz D. Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii. kvj. Eylül 2023;16(3):383-392.
Chicago Küçükkurt, İsmail, Fahriye Kan, Sinan İnce, Hasan Hüseyin Demirel, Erten Akbel, ve Damla Arslan Acaröz. “Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis Akmanii”. Kocatepe Veterinary Journal 16, sy. 3 (Eylül 2023): 383-92.
EndNote Küçükkurt İ, Kan F, İnce S, Demirel HH, Akbel E, Arslan Acaröz D (01 Eylül 2023) Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii. Kocatepe Veterinary Journal 16 3 383–392.
IEEE İ. Küçükkurt, F. Kan, S. İnce, H. H. Demirel, E. Akbel, ve D. Arslan Acaröz, “Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii”, kvj, c. 16, sy. 3, ss. 383–392, 2023.
ISNAD Küçükkurt, İsmail vd. “Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis Akmanii”. Kocatepe Veterinary Journal 16/3 (Eylül 2023), 383-392.
JAMA Küçükkurt İ, Kan F, İnce S, Demirel HH, Akbel E, Arslan Acaröz D. Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii. kvj. 2023;16:383–392.
MLA Küçükkurt, İsmail vd. “Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis Akmanii”. Kocatepe Veterinary Journal, c. 16, sy. 3, 2023, ss. 383-92.
Vancouver Küçükkurt İ, Kan F, İnce S, Demirel HH, Akbel E, Arslan Acaröz D. Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii. kvj. 2023;16(3):383-92.
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