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ANTIOXIDANT RESPONSES TO DROUGHT STRESS IN PENNYROYAL (Mentha pulegium L.)

Yıl 2022, Sayı: 051, 26 - 48, 31.12.2022

Funda Ulusu Kader Tümer Yakup Ulusu

Öz

Mentha pulegium L. (Lamiacea) is a valuable medicinal and aromatic plant found in humid and arid bioclimatic regions of Turkey. Drought stress is a growing concern for the future of agriculture, as well as the most common abiotic stress factor affecting the biochemical processes of plants and seriously damaging crop productivity. The aim of the study was to evaluate the effects of drought stress on the activity of enzymatic antioxidants (polyphenol oxidase - PPO, peroxidase - POD, ascorbate peroxidase - ASPX, catalase - CAT) and some ecophysiological (total chlorophyll content, chlorophyll a and b, carotenoid) responses in M. pulegium grown in pots under greenhouse conditions. In addition, oxidative stress markers were analysed to determine whether drought stress causes oxidative damage in pennyroyal. The plants were exposed to water stress during the 3rd, 6th and 10th days. All enzymatic antioxidant activities of plants under stress were increased compared to control plants. While there was a significant increase in PPO and POD activities in the first days of drought treatment, the prolongation of the treatment period resulted in a significant decrease in these activities. In addition, drought significantly increased lipid peroxidation (294%), hydrogen peroxide (158%) and proline (3172%) content compared to controls. These results show that drought treatment and duration significantly affect antioxidant enzyme activities, lipid peroxidation, hydrogen peroxide and proline content. DPPH (2-diphenyl-1-picrylhydrazyl), ABTS (2,20’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging activity, Fe+2 (FRAP) reducing activity and total phenolic content analysis were performed to analyse the effects of drought stress on antioxidant properties of pennyroyal plant. In addition, the decrease in photosynthetic pigment content in parallel with the prolongation of the drought period due to oxidative damage shows that this valuable medicinal and aromatic plant has low tolerance to drought.

Anahtar Kelimeler

Mentha pulegium Drought stress Enzymatic antioxidants Proline MDA

Teşekkür

The authors declare that they have no conflict of interest.

Kaynakça

  • [1] Hassan, F.A.S. and Ali, E.F., (2014), Impact of different water regimes based on class-A pan on growth, yield and oil content of Coriandrum sativum L. plant. Journal of the Saudi Society of Agricultural Sciences, 13(2), 155-161.
  • [2] Prăvălie, R., Patriche, C., Borrelli, P., Panagos, P., Roșca, B., Dumitraşcu, M. and Bandoc, G., (2021), Arable lands under the pressure of multiple land degradation processes. A global perspective. Environmental Research, 194, 110697.
  • [3] Anjum, S. A., Ashraf, U., Tanveer, M., Khan, I., Hussain, S., Zohaib, A. and Wang, L., (2017), Drought tolerance in three maize cultivars is related to differential osmolyte accumulation, antioxidant defense system, and oxidative damage. Frontiers in Plant Science, 8, 69.
  • [4] Alharby, H.F. and Fahad, S., (2020), Melatonin application enhances biochar efficiency for drought tolerance in maize varieties: Modifications in physio‐biochemical machinery. Agronomy Journal, 112(4), 2826-2847.
  • [5] Foyer, C.H. and Noctor, G., (2005), Oxidant and antioxidant signalling in plants: a re‐evaluation of the concept of oxidative stress in a physiological context. Plant, Cell & Environment, 28(8), 1056-1071.
  • [6] Saleem, M.H., Ali, S., Rehman, M., Hasanuzzaman, M., Rizwan, M., Irshad, S. and Qari, S.H., (2020), Jute: a potential candidate for phytoremediation of metals—a review. Plants, 9(2), 258.
  • [7] Gill, S.S. and Tuteja, N., (2010), Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant physiology and biochemistry, 48(12), 909-930.
  • [8] Anjum, S. A., Wang, L., Farooq, M., Khan, I. and Xue, L., (2011), Methyl jasmonate‐induced alteration in lipid peroxidation, antioxidative defence system and yield in soybean under drought. Journal of Agronomy and Crop Science, 197(4), 296-301.
  • [9] Azarabadi, S., Abdollahi, H., Torabi, M., Salehi, Z. and Nasiri, J., (2017), ROS generation, oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (ASPX) in response to Erwinia amylovora in pear (Pyrus communis L). European Journal of Plant Pathology, 147(2), 279-294.
  • [10] Rehman, M., Liu, L., Bashir, S., Saleem, M.H., Chen, C., Peng, D. and Siddique, K.H., (2019), Influence of rice straw biochar on growth, antioxidant capacity and copper uptake in ramie (Boehmeria nivea L.) grown as forage in aged copper-contaminated soil. Plant Physiology and Biochemistry, 138, 121-129.
  • [11] Reddy, A.R., Chaitanya, K.V. and Vivekanandan, M., (2004), Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of plant physiology, 161(11), 1189-1202.
  • [12] Kamoshita, A., Babu, R. C., Boopathi, N. M. and Fukai, S., (2008), Phenotypic and genotypic analysis of drought-resistance traits for development of rice cultivars adapted to rainfed environments. Field crops research, 109(1-3), 1-23.
  • [13] Muscolo, A., Junker, A., Klukas, C., Weigelt-Fischer, K., Riewe, D. and Altmann, T., (2015), Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions. Journal of experimental botany, 66(18), 5467-5480.
  • [14] Pourghasemian, N., Moradi, R., Naghizadeh, M. and Landberg, T., (2020), Mitigating drought stress in sesame by foliar application of salicylic acid, beeswax waste and licorice extract. Agricultural Water Management, 231, 105997.
  • [15] Mahboubi, M. and Haghi, G., (2008), Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. Journal of ethnopharmacology, 119(2), 325-327.
  • [16] Bakour, M., Campos, M. D.G., Imtara, H. and Lyoussi, B., (2020), Antioxidant content and identification of phenolic/flavonoid compounds in the pollen of fourteen plants using HPLC-DAD. Journal of Apicultural Research, 59(1), 35-41.
  • [17] Di Stasi L.C., Oliveira G.P., Carvalhaes M.A., Queiroz-Junior M. and Tien OS., (2002), Medicinal plants popularly used in the Brazilian tropical Atlantic forest. Fitoterapia 73: 69-91.
  • [18] Mahboubi, M. and Haghi, G., (2008), Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. Journal of ethnopharmacology, 119(2), 325-327.
  • [19] Teixeira B., Marques A., Ramos C., Batista I., and Serrano C., (2012), European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products 36: 81-87.
  • [20] Yumrutas O. and Saygıdeger S.D., (2012), Determination of antioxidant and antimutagenic activities of Phlomis armeniaca and Mentha pulegium. J Appl Pharm Sci 2012: 36-40.
  • [21] Karray‐Bouraoui, N.A.J.O.U.A., Ksouri, R., Falleh, H., Rabhi, M., Jaleel, C.A., Grignon, C. and Lachaal, M., (2010), Effects of environment and development stage on phenolic content and antioxidant activities of Mentha pulegium L. Journal of Food Biochemistry, 34, 79-89.
  • [22] Hassanpour, H., Khavari-Nejad, R.A., Niknam, V., Razavi, K. and Najafi, F., (2014), Effect of penconazole and drought stress on the essential oil composition and gene expression of Mentha pulegium L. (Lamiaceae) at flowering stage. Acta physiologiae plantarum, 36(5), 1167-1175.
  • [23] Azad, N., Rezayian, M., Hassanpour, H., Niknam, V. and Ebrahimzadeh, H., (2021), Physiological Mechanism of Salicylic Acid in Mentha pulegium L. under salinity and drought stress. Brazilian Journal of Botany, 44(2), 359-369.
  • [24] Ulusu, Y., Öztürk, L. and Elmastaş, M., (2017), Antioxidant capacity and cadmium accumulation in parsley seedlings exposed to cadmium stress. Russian journal of plant physiology, 64(6), 883-888.
  • [25] Sharma, P., Jha, A.B., Dubey, R.S. and Pessarakli, M., (2012), Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of botany, 2012.
  • [26] Karabal, E., Yücel, M. and Öktem, H.A., (2003), Antioxidant responses of tolerant and sensitive barley cultivars to boron toxicity. Plant Science, 164(6), 925-933.
  • [27] Flurkey, W.H., (1989), Polypeptide composition and amino-terminal sequence of broad bean polyphenoloxidase. Plant physiology, 91(2), 481-483.
  • [28] Öztürk, L. and Demir, Y., (2003), Effects of putrescine and ethephon on some oxidative stress enzyme activities and proline content in salt stressed spinach leaves. Plant Growth Regulation, 40(1), 89-95.
  • [29] Sreenivasulu, N., Ramanjulu, S., Ramachandra-Kini, K., Prakash, H.S., Shekar-Shetty, H., Savithri, H.S. and Sudhakar, C., (1999), Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance. Plant Science, 141(1), 1-9.
  • [30] Velikova, V., Yordanov, I. and Edreva, A., (2000), Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant science, 151(1), 59-66.
  • [31] Arnon, D.I., (1949), Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology, 24(1), 1.
  • [32] Witham, F.H., Blaydes, D.F. and Devlin, R.M., (1971), Experiments in plant physiology. Van Nostrand Reinhold Co, New York, c1971
  • [33] Bradford, M.M., (1976), A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.
  • [34] Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C., (1999), Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237.
  • [35] Sharma, O. P. and Bhat, T. K., (2009), DPPH antioxidant assay revisited. Food chemistry, 113(4), 1202-1205.
  • [36] Benzie, I.F. and Strain, J.J., (1996), The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70-76.
  • [37] Duncan, D.B., (1955), Multiple range and multiple F tests. Biometrics, 11(1), 1-42.
  • [38] Asghari, B., Khademian, R. and Sedaghati, B., (2020), Plant growth promoting rhizobacteria (PGPR) confer drought resistance and stimulate biosynthesis of secondary metabolites in pennyroyal (Mentha pulegium L.) under water shortage condition. Scientia Horticulturae, 263, 109132.
  • [39] Mafakheri, A., Siosemardeh, A., Bahramnejad, B., Struik, P.C. and Sohrabi, Y., (2011), Effect of drought stress and subsequent recovery on protein, carbohydrate contents, catalase and peroxidase activities in three chickpea (Cicer arietinum) cultivars. Australian Journal of Crop Science, 5(10), 1255-1260.
  • [40] Mohammadi, A., Habibi, D., Rohami, M. and Mafakheri, S., (2011), Effect of drought stress on antioxidant enzymes activity of some chickpea cultivars. Am-Euras. J. Agric. Environ. Sci, 11(6), 782-785.
  • [41] Lum, M.S., Hanafi, M.M., Rafii, Y.M. and Akmar, A.S.N., (2014), Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice. J. Anim. Plant Sci, 24(5), 1487-1493.
  • [42] Sofo, A., Dichio, B., Xiloyannis, C. and Masia, A., (2005), Antioxidant defenses in olive trees during drought stress: changes in activity of some antioxidant enzymes. Functional Plant Biology, 32(1), 45-53.
  • [43] Chakraborty, U. and Pradhan, B., (2012), Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation. Brazilian Journal of Plant Physiology, 24, 117-130.
  • [44] Khazaei, Z. and Estaji, A., (2020), Effect of foliar application of ascorbic acid on sweet pepper (Capsicum annuum) plants under drought stress. Acta Physiologiae Plantarum, 42(7), 1-12.
  • [45] Naderi, S., Fakheri, B.A., Maali-Amiri, R. and Mahdinezhad, N., (2020), Tolerance responses in wheat landrace Bolani are related to enhanced metabolic adjustments under drought stress. Plant Physiology and Biochemistry, 150, 244-253.
  • [46] Raven, E.L., (2003), Understanding functional diversity and substrate specificity in haem peroxidases: what can we learn from ascorbate peroxidase? Natural product reports, 20(4), 367-381.
  • [47] Hassanpour, H., Khavari-Nejad, R.A., Niknam, V., Najafi, F. and Razavi, K., (2012), Effects of penconazole and water deficit stress on physiological and antioxidative responses in pennyroyal (Mentha pulegium L.). Acta physiologiae plantarum, 34(4), 1537-1549.
  • [48] Branch, K., (2009), Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. American Journal of Agricultural and Biological Sciences, 4(3), 215-223.
  • [49] Chugh, V., Kaur, N. and Gupta, A.K., (2011), Evaluation of oxidative stress tolerance in maize (Zea mays L.) seedlings in response to drought. Indian J Biochem Biophys, 48(1), 47-53.
  • [50] Han, Y. H., (1997), Effect of high temperature and/or drought stress on the activities of SOD and POD of intact leaves in two soybean (G. max) cultivars. Soybean Genetics Newsletter, 24, 39-40
  • [51] Jaleel, C.A., Ragupathi, G.O.P.I. and Panneerselvam, R., (2008), Biochemical alterations in white yam (Dioscorea rotundata Poir.) under triazole fungicides: impacts on tuber quality. Czech J. Food Sci. Vol, 26(4), 297-307.
  • [52] Liszkay, A., Kenk, B. and Schopfer, P., (2003), Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth. Planta, 217(4), 658-667.
  • [53] Passardi, F., Penel, C. and Dunand, C., (2004), Performing the paradoxical: how plant peroxidases modify the cell wall. Trends in plant science, 9(11), 534-540.
  • [54] Jaleel, C.A., Manivannan, P., Kishorekumar, A., Sankar, B., Gopi, R., Somasundaram, R. and Panneerselvam, R., (2007), Alterations in osmoregulation, antioxidant enzymes and indole alkaloid levels in Catharanthus roseus exposed to water deficit. Colloids and Surfaces B: Biointerfaces, 59(2), 150-157.
  • [55] Mittler, R., (2002), Oxidative stress, antioxidants and stress tolerance. Trends in plant science, 7(9), 405-410.
  • [56] Tlili, N., Elfalleh, W., Hannachi, H., Yahia, Y., Khaldi, A., Ferchichi, A. and Nasri, N., (2013), Screening of natural antioxidants from selected medicinal plants. International journal of food properties, 16(5), 1117-1126.
  • [57] Mostajeran, A. and Rahimi-Eichi, V., (2009), Effects of drought stress on growth and yield of rice (Oryza sativa L.) cultivars and accumulation of proline and soluble sugars in sheath and blades of their different ages leaves. Agric. & Environ. Sci, 5(2), 264-272.
  • [58] Dien, D.C., Mochizuki, T. and Yamakawa, T., (2019), Effect of various drought stresses and subsequent recovery on proline, total soluble sugar and starch metabolisms in Rice (Oryza sativa L.) varieties. Plant Production Science, 22(4), 530-545.
  • [59] Parida, A.K., Dagaonkar, V.S., Phalak, M.S. and Aurangabadkar, L.P., (2008), Differential responses of the enzymes involved in proline biosynthesis and degradation in drought tolerant and sensitive cotton genotypes during drought stress and recovery. Acta Physiologiae Plantarum, 30(5), 619-627.
  • [60] Man, D., Bao, Y.X., Han, L.B. and Zhang, X., (2011), Drought tolerance associated with proline and hormone metabolism in two tall fescue cultivars. HortScience, 46(7), 1027-1032.
  • [61] Sultan, M.A.R.F., Hui, L., Yang, L.J. and Xian, Z.H., (2012), Assessment of drought tolerance of some Triticum L. species through physiological indices. Czech Journal of Genetics and Plant Breeding, 48(4), 178-184.
  • [62] Siripornadulsil, S., Traina, S., Verma, D.P.S. and Sayre, R.T., (2002), Molecular mechanisms of proline-mediated tolerance to toxic heavy metals in transgenic microalgae. The Plant Cell, 14(11), 2837-2847.
  • [63] Mattioli, R., Marchese, D., D’Angeli, S., Altamura, M.M., Costantino, P. and Trovato, M., (2008), Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis. Plant Molecular Biology, 66(3), 277-288.
  • [64] Miller, G., Honig, A., Stein, H., Suzuki, N., Mittler, R. and Zilberstein, A., (2009), Unraveling Δ1-pyrroline-5-carboxylate-proline cycle in plants by uncoupled expression of proline oxidation enzymes. Journal of Biological Chemistry, 284(39), 26482-26492.
  • [65] Kishor, P.K., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu, K.R., Rao, K.S. and Sreenivasulu, N., (2005), Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Current science, 424-438.
  • [66] Furlan, A.L., Bianucci, E., Giordano, W., Castro, S. and Becker, D.F., (2020), Proline metabolic dynamics and implications in drought tolerance of peanut plants. Plant Physiology and Biochemistry, 151, 566-578.
  • [67] Szabados, L., Savouré, A., (2010), Proline: a multifunctional amino acid. Trends in plant science, 15(2), 89-97.
  • [68] Natarajan, S.K., Zhu, W., Liang, X., Zhang, L., Demers, A.J., Zimmerman, M.C. and Becker, D.F., (2012), Proline dehydrogenase is essential for proline protection against hydrogen peroxide-induced cell death. Free radical biology and medicine, 53(5), 1181-1191.
  • [69] Kavı Kıshor, P.B. and Sreenivasulu, N., (2014), Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue?. Plant, Cell & Environment, 37(2), 300-311.
  • [70] Dar, M.I., Naikoo, M.I., Rehman, F., Naushin, F. and Khan, F.A., (2016), Proline accumulation in plants: roles in stress tolerance and plant development. In Osmolytes and plants acclimation to changing environment. Emerging Omics Technologies (pp. 155-166). Springer, New Delhi.
  • [71] Siswoyo, T.A., Arum, L.S., Sanjaya, B.R.L. and Aisyah, Z.S., (2021), The growth responses and antioxidant capabilities of melinjo (Gnetum gnemon L.) in different durations of drought stress. Annals of Agricultural Sciences, 66(1), 81-86.
  • [72] Wang F., Yu G. and Liu P., (2019), Transporter-mediated subcellular distribution in the metabolism and signaling of jasmonates. Front Plant Sci 10, 390.
  • [73] Arora, A., Sairam, R.K. and Srivastava, G.C., (2002), Oxidative stress and antioxidative system in plants. Current Science, 82(10), 1227-1238.
  • [74] Smirnoff, N., (1993), The role of active oxygen in the response of plants to water deficit and desiccation. New phytologist, 125, 27-58.
  • [75] Mafakheri, A., Siosemardeh, A.F., Bahramnejad, B., Struik, P.C. and Sohrabi, Y., (2010), Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science, 4(8), 580-585.
  • [76] Sohrabi, Y., Heidari, G., Weisany, W., Golezani, K.G. and Mohammadi, K., (2012), Changes of antioxidative enzymes, lipid peroxidation and chlorophyll content in chickpea types colonized by different Glomus species under drought stress. Symbiosis, 56(1), 5-18.
  • [77] Khayatnezhad, M. and Gholamin, R., (2012), The effect of drought stress on leaf chlorophyll content and stress resistance in maize cultivars (Zea mays). African Journal of Microbiology Research, 6(12), 2844-2848.
  • [78] Meher, P.S., Reddy, K.A. and Rao, D.M., (2018), Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biological Sciences, 25(2), 285.
  • [79] Michaletti, A., Naghavi, M. R., Toorchi, M., Zolla, L., Rinalducci, S., (2018), Metabolomics and proteomics reveal drought-stress responses of leaf tissues from spring-wheat. Scientific reports, 8(1), 1-18.
  • [80] Mohammadkhani, N. and Heidari, R., (2008), Effects of drought stress on soluble proteins in two maize varieties. Turkish Journal of Biology, 32(1), 23-30.
  • [81] Khoyerdi, F.F., Shamshiri, M. H. and Estaji, A., (2016), Changes in some physiological and osmotic parameters of several pistachio genotypes under drought stress. Scientia horticulturae, 198, 44-51.
  • [82] Akhzari, D. and Pessarakli, M., (2016), Effect of drought stress on total protein, essential oil content, and physiological traits of Levisticum officinale Koch. Journal of Plant Nutrition, 39(10), 1365-1371.
  • [83] Kopta, T., Sekara, A., Pokluda, R., Ferby, V. and Caruso, G., (2020), Screening of chilli pepper genotypes as a source of capsaicinoids and antioxidants under conditions of simulated drought stress. Plants, 9(3), 364.
  • [84] Sarker, U. and Oba, S., (2018), Drought stress enhances nutritional and bioactive compounds, phenolic acids and antioxidant capacity of Amaranthus leafy vegetable. BMC Plant biology, 18(1), 1-15.
  • [85] Sahitya, U.L., Krishna, M.S.R., Deepthi, R., Prasad, G.S. and Kasim, D., (2018), Seed antioxidants interplay with drought stress tolerance indices in chilli (Capsicum annuum L.) seedlings. BioMed Research International, 2018.

Yıl 2022, Sayı: 051, 26 - 48, 31.12.2022

Funda Ulusu Kader Tümer Yakup Ulusu

Öz

Kaynakça

  • [1] Hassan, F.A.S. and Ali, E.F., (2014), Impact of different water regimes based on class-A pan on growth, yield and oil content of Coriandrum sativum L. plant. Journal of the Saudi Society of Agricultural Sciences, 13(2), 155-161.
  • [2] Prăvălie, R., Patriche, C., Borrelli, P., Panagos, P., Roșca, B., Dumitraşcu, M. and Bandoc, G., (2021), Arable lands under the pressure of multiple land degradation processes. A global perspective. Environmental Research, 194, 110697.
  • [3] Anjum, S. A., Ashraf, U., Tanveer, M., Khan, I., Hussain, S., Zohaib, A. and Wang, L., (2017), Drought tolerance in three maize cultivars is related to differential osmolyte accumulation, antioxidant defense system, and oxidative damage. Frontiers in Plant Science, 8, 69.
  • [4] Alharby, H.F. and Fahad, S., (2020), Melatonin application enhances biochar efficiency for drought tolerance in maize varieties: Modifications in physio‐biochemical machinery. Agronomy Journal, 112(4), 2826-2847.
  • [5] Foyer, C.H. and Noctor, G., (2005), Oxidant and antioxidant signalling in plants: a re‐evaluation of the concept of oxidative stress in a physiological context. Plant, Cell & Environment, 28(8), 1056-1071.
  • [6] Saleem, M.H., Ali, S., Rehman, M., Hasanuzzaman, M., Rizwan, M., Irshad, S. and Qari, S.H., (2020), Jute: a potential candidate for phytoremediation of metals—a review. Plants, 9(2), 258.
  • [7] Gill, S.S. and Tuteja, N., (2010), Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant physiology and biochemistry, 48(12), 909-930.
  • [8] Anjum, S. A., Wang, L., Farooq, M., Khan, I. and Xue, L., (2011), Methyl jasmonate‐induced alteration in lipid peroxidation, antioxidative defence system and yield in soybean under drought. Journal of Agronomy and Crop Science, 197(4), 296-301.
  • [9] Azarabadi, S., Abdollahi, H., Torabi, M., Salehi, Z. and Nasiri, J., (2017), ROS generation, oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (ASPX) in response to Erwinia amylovora in pear (Pyrus communis L). European Journal of Plant Pathology, 147(2), 279-294.
  • [10] Rehman, M., Liu, L., Bashir, S., Saleem, M.H., Chen, C., Peng, D. and Siddique, K.H., (2019), Influence of rice straw biochar on growth, antioxidant capacity and copper uptake in ramie (Boehmeria nivea L.) grown as forage in aged copper-contaminated soil. Plant Physiology and Biochemistry, 138, 121-129.
  • [11] Reddy, A.R., Chaitanya, K.V. and Vivekanandan, M., (2004), Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of plant physiology, 161(11), 1189-1202.
  • [12] Kamoshita, A., Babu, R. C., Boopathi, N. M. and Fukai, S., (2008), Phenotypic and genotypic analysis of drought-resistance traits for development of rice cultivars adapted to rainfed environments. Field crops research, 109(1-3), 1-23.
  • [13] Muscolo, A., Junker, A., Klukas, C., Weigelt-Fischer, K., Riewe, D. and Altmann, T., (2015), Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions. Journal of experimental botany, 66(18), 5467-5480.
  • [14] Pourghasemian, N., Moradi, R., Naghizadeh, M. and Landberg, T., (2020), Mitigating drought stress in sesame by foliar application of salicylic acid, beeswax waste and licorice extract. Agricultural Water Management, 231, 105997.
  • [15] Mahboubi, M. and Haghi, G., (2008), Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. Journal of ethnopharmacology, 119(2), 325-327.
  • [16] Bakour, M., Campos, M. D.G., Imtara, H. and Lyoussi, B., (2020), Antioxidant content and identification of phenolic/flavonoid compounds in the pollen of fourteen plants using HPLC-DAD. Journal of Apicultural Research, 59(1), 35-41.
  • [17] Di Stasi L.C., Oliveira G.P., Carvalhaes M.A., Queiroz-Junior M. and Tien OS., (2002), Medicinal plants popularly used in the Brazilian tropical Atlantic forest. Fitoterapia 73: 69-91.
  • [18] Mahboubi, M. and Haghi, G., (2008), Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. Journal of ethnopharmacology, 119(2), 325-327.
  • [19] Teixeira B., Marques A., Ramos C., Batista I., and Serrano C., (2012), European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products 36: 81-87.
  • [20] Yumrutas O. and Saygıdeger S.D., (2012), Determination of antioxidant and antimutagenic activities of Phlomis armeniaca and Mentha pulegium. J Appl Pharm Sci 2012: 36-40.
  • [21] Karray‐Bouraoui, N.A.J.O.U.A., Ksouri, R., Falleh, H., Rabhi, M., Jaleel, C.A., Grignon, C. and Lachaal, M., (2010), Effects of environment and development stage on phenolic content and antioxidant activities of Mentha pulegium L. Journal of Food Biochemistry, 34, 79-89.
  • [22] Hassanpour, H., Khavari-Nejad, R.A., Niknam, V., Razavi, K. and Najafi, F., (2014), Effect of penconazole and drought stress on the essential oil composition and gene expression of Mentha pulegium L. (Lamiaceae) at flowering stage. Acta physiologiae plantarum, 36(5), 1167-1175.
  • [23] Azad, N., Rezayian, M., Hassanpour, H., Niknam, V. and Ebrahimzadeh, H., (2021), Physiological Mechanism of Salicylic Acid in Mentha pulegium L. under salinity and drought stress. Brazilian Journal of Botany, 44(2), 359-369.
  • [24] Ulusu, Y., Öztürk, L. and Elmastaş, M., (2017), Antioxidant capacity and cadmium accumulation in parsley seedlings exposed to cadmium stress. Russian journal of plant physiology, 64(6), 883-888.
  • [25] Sharma, P., Jha, A.B., Dubey, R.S. and Pessarakli, M., (2012), Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of botany, 2012.
  • [26] Karabal, E., Yücel, M. and Öktem, H.A., (2003), Antioxidant responses of tolerant and sensitive barley cultivars to boron toxicity. Plant Science, 164(6), 925-933.
  • [27] Flurkey, W.H., (1989), Polypeptide composition and amino-terminal sequence of broad bean polyphenoloxidase. Plant physiology, 91(2), 481-483.
  • [28] Öztürk, L. and Demir, Y., (2003), Effects of putrescine and ethephon on some oxidative stress enzyme activities and proline content in salt stressed spinach leaves. Plant Growth Regulation, 40(1), 89-95.
  • [29] Sreenivasulu, N., Ramanjulu, S., Ramachandra-Kini, K., Prakash, H.S., Shekar-Shetty, H., Savithri, H.S. and Sudhakar, C., (1999), Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance. Plant Science, 141(1), 1-9.
  • [30] Velikova, V., Yordanov, I. and Edreva, A., (2000), Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant science, 151(1), 59-66.
  • [31] Arnon, D.I., (1949), Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology, 24(1), 1.
  • [32] Witham, F.H., Blaydes, D.F. and Devlin, R.M., (1971), Experiments in plant physiology. Van Nostrand Reinhold Co, New York, c1971
  • [33] Bradford, M.M., (1976), A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.
  • [34] Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C., (1999), Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237.
  • [35] Sharma, O. P. and Bhat, T. K., (2009), DPPH antioxidant assay revisited. Food chemistry, 113(4), 1202-1205.
  • [36] Benzie, I.F. and Strain, J.J., (1996), The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70-76.
  • [37] Duncan, D.B., (1955), Multiple range and multiple F tests. Biometrics, 11(1), 1-42.
  • [38] Asghari, B., Khademian, R. and Sedaghati, B., (2020), Plant growth promoting rhizobacteria (PGPR) confer drought resistance and stimulate biosynthesis of secondary metabolites in pennyroyal (Mentha pulegium L.) under water shortage condition. Scientia Horticulturae, 263, 109132.
  • [39] Mafakheri, A., Siosemardeh, A., Bahramnejad, B., Struik, P.C. and Sohrabi, Y., (2011), Effect of drought stress and subsequent recovery on protein, carbohydrate contents, catalase and peroxidase activities in three chickpea (Cicer arietinum) cultivars. Australian Journal of Crop Science, 5(10), 1255-1260.
  • [40] Mohammadi, A., Habibi, D., Rohami, M. and Mafakheri, S., (2011), Effect of drought stress on antioxidant enzymes activity of some chickpea cultivars. Am-Euras. J. Agric. Environ. Sci, 11(6), 782-785.
  • [41] Lum, M.S., Hanafi, M.M., Rafii, Y.M. and Akmar, A.S.N., (2014), Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice. J. Anim. Plant Sci, 24(5), 1487-1493.
  • [42] Sofo, A., Dichio, B., Xiloyannis, C. and Masia, A., (2005), Antioxidant defenses in olive trees during drought stress: changes in activity of some antioxidant enzymes. Functional Plant Biology, 32(1), 45-53.
  • [43] Chakraborty, U. and Pradhan, B., (2012), Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation. Brazilian Journal of Plant Physiology, 24, 117-130.
  • [44] Khazaei, Z. and Estaji, A., (2020), Effect of foliar application of ascorbic acid on sweet pepper (Capsicum annuum) plants under drought stress. Acta Physiologiae Plantarum, 42(7), 1-12.
  • [45] Naderi, S., Fakheri, B.A., Maali-Amiri, R. and Mahdinezhad, N., (2020), Tolerance responses in wheat landrace Bolani are related to enhanced metabolic adjustments under drought stress. Plant Physiology and Biochemistry, 150, 244-253.
  • [46] Raven, E.L., (2003), Understanding functional diversity and substrate specificity in haem peroxidases: what can we learn from ascorbate peroxidase? Natural product reports, 20(4), 367-381.
  • [47] Hassanpour, H., Khavari-Nejad, R.A., Niknam, V., Najafi, F. and Razavi, K., (2012), Effects of penconazole and water deficit stress on physiological and antioxidative responses in pennyroyal (Mentha pulegium L.). Acta physiologiae plantarum, 34(4), 1537-1549.
  • [48] Branch, K., (2009), Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. American Journal of Agricultural and Biological Sciences, 4(3), 215-223.
  • [49] Chugh, V., Kaur, N. and Gupta, A.K., (2011), Evaluation of oxidative stress tolerance in maize (Zea mays L.) seedlings in response to drought. Indian J Biochem Biophys, 48(1), 47-53.
  • [50] Han, Y. H., (1997), Effect of high temperature and/or drought stress on the activities of SOD and POD of intact leaves in two soybean (G. max) cultivars. Soybean Genetics Newsletter, 24, 39-40
  • [51] Jaleel, C.A., Ragupathi, G.O.P.I. and Panneerselvam, R., (2008), Biochemical alterations in white yam (Dioscorea rotundata Poir.) under triazole fungicides: impacts on tuber quality. Czech J. Food Sci. Vol, 26(4), 297-307.
  • [52] Liszkay, A., Kenk, B. and Schopfer, P., (2003), Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth. Planta, 217(4), 658-667.
  • [53] Passardi, F., Penel, C. and Dunand, C., (2004), Performing the paradoxical: how plant peroxidases modify the cell wall. Trends in plant science, 9(11), 534-540.
  • [54] Jaleel, C.A., Manivannan, P., Kishorekumar, A., Sankar, B., Gopi, R., Somasundaram, R. and Panneerselvam, R., (2007), Alterations in osmoregulation, antioxidant enzymes and indole alkaloid levels in Catharanthus roseus exposed to water deficit. Colloids and Surfaces B: Biointerfaces, 59(2), 150-157.
  • [55] Mittler, R., (2002), Oxidative stress, antioxidants and stress tolerance. Trends in plant science, 7(9), 405-410.
  • [56] Tlili, N., Elfalleh, W., Hannachi, H., Yahia, Y., Khaldi, A., Ferchichi, A. and Nasri, N., (2013), Screening of natural antioxidants from selected medicinal plants. International journal of food properties, 16(5), 1117-1126.
  • [57] Mostajeran, A. and Rahimi-Eichi, V., (2009), Effects of drought stress on growth and yield of rice (Oryza sativa L.) cultivars and accumulation of proline and soluble sugars in sheath and blades of their different ages leaves. Agric. & Environ. Sci, 5(2), 264-272.
  • [58] Dien, D.C., Mochizuki, T. and Yamakawa, T., (2019), Effect of various drought stresses and subsequent recovery on proline, total soluble sugar and starch metabolisms in Rice (Oryza sativa L.) varieties. Plant Production Science, 22(4), 530-545.
  • [59] Parida, A.K., Dagaonkar, V.S., Phalak, M.S. and Aurangabadkar, L.P., (2008), Differential responses of the enzymes involved in proline biosynthesis and degradation in drought tolerant and sensitive cotton genotypes during drought stress and recovery. Acta Physiologiae Plantarum, 30(5), 619-627.
  • [60] Man, D., Bao, Y.X., Han, L.B. and Zhang, X., (2011), Drought tolerance associated with proline and hormone metabolism in two tall fescue cultivars. HortScience, 46(7), 1027-1032.
  • [61] Sultan, M.A.R.F., Hui, L., Yang, L.J. and Xian, Z.H., (2012), Assessment of drought tolerance of some Triticum L. species through physiological indices. Czech Journal of Genetics and Plant Breeding, 48(4), 178-184.
  • [62] Siripornadulsil, S., Traina, S., Verma, D.P.S. and Sayre, R.T., (2002), Molecular mechanisms of proline-mediated tolerance to toxic heavy metals in transgenic microalgae. The Plant Cell, 14(11), 2837-2847.
  • [63] Mattioli, R., Marchese, D., D’Angeli, S., Altamura, M.M., Costantino, P. and Trovato, M., (2008), Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis. Plant Molecular Biology, 66(3), 277-288.
  • [64] Miller, G., Honig, A., Stein, H., Suzuki, N., Mittler, R. and Zilberstein, A., (2009), Unraveling Δ1-pyrroline-5-carboxylate-proline cycle in plants by uncoupled expression of proline oxidation enzymes. Journal of Biological Chemistry, 284(39), 26482-26492.
  • [65] Kishor, P.K., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu, K.R., Rao, K.S. and Sreenivasulu, N., (2005), Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Current science, 424-438.
  • [66] Furlan, A.L., Bianucci, E., Giordano, W., Castro, S. and Becker, D.F., (2020), Proline metabolic dynamics and implications in drought tolerance of peanut plants. Plant Physiology and Biochemistry, 151, 566-578.
  • [67] Szabados, L., Savouré, A., (2010), Proline: a multifunctional amino acid. Trends in plant science, 15(2), 89-97.
  • [68] Natarajan, S.K., Zhu, W., Liang, X., Zhang, L., Demers, A.J., Zimmerman, M.C. and Becker, D.F., (2012), Proline dehydrogenase is essential for proline protection against hydrogen peroxide-induced cell death. Free radical biology and medicine, 53(5), 1181-1191.
  • [69] Kavı Kıshor, P.B. and Sreenivasulu, N., (2014), Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue?. Plant, Cell & Environment, 37(2), 300-311.
  • [70] Dar, M.I., Naikoo, M.I., Rehman, F., Naushin, F. and Khan, F.A., (2016), Proline accumulation in plants: roles in stress tolerance and plant development. In Osmolytes and plants acclimation to changing environment. Emerging Omics Technologies (pp. 155-166). Springer, New Delhi.
  • [71] Siswoyo, T.A., Arum, L.S., Sanjaya, B.R.L. and Aisyah, Z.S., (2021), The growth responses and antioxidant capabilities of melinjo (Gnetum gnemon L.) in different durations of drought stress. Annals of Agricultural Sciences, 66(1), 81-86.
  • [72] Wang F., Yu G. and Liu P., (2019), Transporter-mediated subcellular distribution in the metabolism and signaling of jasmonates. Front Plant Sci 10, 390.
  • [73] Arora, A., Sairam, R.K. and Srivastava, G.C., (2002), Oxidative stress and antioxidative system in plants. Current Science, 82(10), 1227-1238.
  • [74] Smirnoff, N., (1993), The role of active oxygen in the response of plants to water deficit and desiccation. New phytologist, 125, 27-58.
  • [75] Mafakheri, A., Siosemardeh, A.F., Bahramnejad, B., Struik, P.C. and Sohrabi, Y., (2010), Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science, 4(8), 580-585.
  • [76] Sohrabi, Y., Heidari, G., Weisany, W., Golezani, K.G. and Mohammadi, K., (2012), Changes of antioxidative enzymes, lipid peroxidation and chlorophyll content in chickpea types colonized by different Glomus species under drought stress. Symbiosis, 56(1), 5-18.
  • [77] Khayatnezhad, M. and Gholamin, R., (2012), The effect of drought stress on leaf chlorophyll content and stress resistance in maize cultivars (Zea mays). African Journal of Microbiology Research, 6(12), 2844-2848.
  • [78] Meher, P.S., Reddy, K.A. and Rao, D.M., (2018), Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biological Sciences, 25(2), 285.
  • [79] Michaletti, A., Naghavi, M. R., Toorchi, M., Zolla, L., Rinalducci, S., (2018), Metabolomics and proteomics reveal drought-stress responses of leaf tissues from spring-wheat. Scientific reports, 8(1), 1-18.
  • [80] Mohammadkhani, N. and Heidari, R., (2008), Effects of drought stress on soluble proteins in two maize varieties. Turkish Journal of Biology, 32(1), 23-30.
  • [81] Khoyerdi, F.F., Shamshiri, M. H. and Estaji, A., (2016), Changes in some physiological and osmotic parameters of several pistachio genotypes under drought stress. Scientia horticulturae, 198, 44-51.
  • [82] Akhzari, D. and Pessarakli, M., (2016), Effect of drought stress on total protein, essential oil content, and physiological traits of Levisticum officinale Koch. Journal of Plant Nutrition, 39(10), 1365-1371.
  • [83] Kopta, T., Sekara, A., Pokluda, R., Ferby, V. and Caruso, G., (2020), Screening of chilli pepper genotypes as a source of capsaicinoids and antioxidants under conditions of simulated drought stress. Plants, 9(3), 364.
  • [84] Sarker, U. and Oba, S., (2018), Drought stress enhances nutritional and bioactive compounds, phenolic acids and antioxidant capacity of Amaranthus leafy vegetable. BMC Plant biology, 18(1), 1-15.
  • [85] Sahitya, U.L., Krishna, M.S.R., Deepthi, R., Prasad, G.S. and Kasim, D., (2018), Seed antioxidants interplay with drought stress tolerance indices in chilli (Capsicum annuum L.) seedlings. BioMed Research International, 2018.
Toplam 85 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Research Articles
Yazarlar

Funda Ulusu 0000-0002-0321-2602

Kader Tümer 0000-0001-6392-0785

Yakup Ulusu 0000-0002-8755-2822

Yayımlanma Tarihi 31 Aralık 2022
Gönderilme Tarihi 21 Temmuz 2022
Yayımlandığı Sayı Yıl 2022 Sayı: 051

Kaynak Göster

IEEE F. Ulusu, K. Tümer, ve Y. Ulusu, “ANTIOXIDANT RESPONSES TO DROUGHT STRESS IN PENNYROYAL (Mentha pulegium L.)”, JSR-A, sy. 051, ss. 26–48, Aralık 2022.
 Kapak Resmi İndir