Araştırma Makalesi
Phaseolus vulgaris’te Genomik Kararsızlık ve DNA Metilasyonunu İndükleyen Piklorama karşı Putresinin Koruyucu Rolü
Öz
Pikloram (4-amino-3-5-6-trikloropikolinik asit) önemli bir sentetik oksin olup, tarımda en çok kullanılan
herbisitlerden biridir. Pikloram kullanımı, ekosistem ve insan sağlığı için potansiyel bir tehlike oluşturmaktadır.
Bir poliamin çeşidi olan putresinin bitki metabolizmasında; membran stabilitesinin korunması, serbest radikallerin
uzaklaştırılması ve nükleik asit ve protein sentezi gibi rolü vardır. Bu çalışma, pikloram uygulanan Phaseolus
vulgaris’ teki DNA hasar düzeyleri ve DNA metilasyon değişiklikleri ile putresinin bu değişimler üzerinde
koruyucu etkisinin olup olmadığının belirlenmesini amaçlamıştır. DNA hasar düzeylerini ve DNA metilasyon
değişimlerini belirlemek için Rastgele Çoğaltılmış Polimorfik DNA (RAPD) ve Çift Restriksiyon Enzim Kesimi-
Rastgele Çoğaltım (CRED-RA) kullanılmıştır. Sonuçlar, tüm pikloram dozlarının (5, 10, 20 ve 40 mg L-1) RAPD
profil değişikliklerini (DNA hasarının) artırdığını ve Genomik Kararlılık Stabilitesini (GTS) azalttığını ve ayrıca
DNA hipometilasyonunun oluştuğunu göstermiştir. Bununla birlikte, kullanılan tüm putresin konsantrasyonları
(0.01, 0.1 ve 1 ppm) pikloramın bu zararlı etkilerini azaltmıştır. Sonuç olarak, putresin, bitkilerdeki kimyasal
mutajenlere karşı genotoksik hasarı azaltmak için bir alternatif olabilir.
Anahtar Kelimeler
Kaynakça
- Aydin M, Arslan E, Taspinar MS, Karadayi G, Agar G, 2016. Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.). Biotechnology and Biotechnological Equipment, 30: 1082-1089.
- Balague C, Sturtz N, Duffard R, Evangelista de Duffard AM, 2001. Effect of 2,4- dichlorophenoxyacetic acid herbicide on Escherichia coli growth, chemical composition, and cellular envelope. Environmental Toxicology, 16: 43–53.
- Correia S, Lopes ML, Canhoto JM, 2011. Somatic embryogenesis induction system for cloning an adult Cyphomandra betacea (Cav.) Sendt. (tamarillo). Trees, 25: 1009-1020.
- D’Agostino L, Di Pietro M, Di Luccia A, 2005. Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation. FEBS Journal, 272: 3777–3787.
- Duchnowicz P, Koter M, 2003. Damage to the erythrocyte membrane caused by chlorophenoxyacetic herbicides. Cellular and Molecular Biology Letters, 8: 25–30.
- Feng SJ, Liu XS, Tao H, Tan SK, Chu SS, Oono Y, Zhang XD, Chen J, Yang ZM, 2016. Variation of DNA methylation patterns associated with gene expression in rice (Oryza sativa) exposed to cadmium. Plant Cell and Environment, 39: 2629-2649.
- Grativol C, Hemerly AS, Ferreira PCG, 2012. Genetic and epigenetic regulation of stress responses in natural plant populations. Biochimica et Biophysica Acta, 1819: 176–185.
- Khan AU, Di-Mascio P, Medeiros MHG, Wilson T, 1992. Spermine and spermidine protection of plasmid DNA against single-strand breaks induced by singlet oxygen. Proceedings of the National Academy of Science of the United States of America, 89: 11428-11430.
- Leljak-Levanic D, Bauer N, Mihaljevic S, Jelaska S, 2004. Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L. Plant Cell Reports, 23: 120–127.
- Miyamoto S, Kashiwagi K, Watanabe S, Igarashi K, 1993. Estimation of polyamine distribution and polyamine stimulation of protein synthesis in Escherichia coli. Archives of Biochemistry and Biophysics, 300: 63-68.
- Mohammed KB, Ma TH, 1999. Tradescantia-micronucleus and -stamen hair mutation assays on genotoxicity of the gaseous and liquid forms of pesticides. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 426: 193-199.
- Oakes DJ, Webster WS, Brown Woodman PDC, Ritchie HE, 2002. Testicular changes induced by chronic exposure to the herbicide formulation, Tordon 75D® (2,4-dichlorophenoxyacetic acid and picloram) in rats. Reproductive Toxicology, 16: 281-289.
- Olea-Popelka F, Mclean DM, Horsman J, Almquist K, Bramdle JE, Hall C, 2005. Increasing expression of an anti-picloram single-chain variable fragment (ScFv) antibody and resistance to picloram in transgenic tobacco (Nicotiana tabacum). Journal of Agricultural and Food Chemistry, 53: 6683-6690.
- Ruiz-Herrera J, Ruiz-Medrano R, Dominguez A, 1995. Selective inhibition of cytosine DNA methylases by polyamines. FEBS Letters, 357: 192-196.
- Salvi ND, George L, Eapen S, 2001. Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell, Tissue and Organ Culture, 66: 113-119.
- Sawamura S, Jackson WT, 1968. Cytological studies in vivo of picloram, pyriclor, trifluralin, 2,3,6-TBA, 2,3,5,6-TBA and nitralin. Cytologia, 33: 545–554.
- Sung S, Amasino RM, 2004. Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3. Nature, 427: 159-164.
- Taspinar MS, Agar G, Yildirim N, Sunar S, Aksakal O, Bozari S, 2009. Evaluation of selenium effect on cadmium genotoxicity in Vicia faba using RAPD. Journal of Food, Agriculture and Environment, 7: 857-860.
- Temel A, Gozukirmizi N, 2013. Analysis of retrotransposition and DNA metyhlation in barley callus culture. Acta Biologica Hungarica, 64: 86-95.
- Wang W, Qiao Q, Sun F, Wang Y, Xu D, Li Z, Fu B, 2016. Genome-wide differences in DNA methylation changes in two contrasting rice genotypes in response to drought conditions. Frontiers in Plant Science, 7: 1675.
- Yildirim N, Agar G, Taspinar MS, Turan M, Aydin M, Arslan E, 2014. Protective role of humic acids against dicamba-induced genotoxicity and DNA methylation in Phaseolus vulgaris L. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 64: 141-148.
- Zeid IM, Shedeed ZA, 2006. Response of alfalfa to putrescine treatment under drought stress. Biologia Plantarum, 50: 635-640.
Yıl 2017,
Cilt: 7 Sayı: 4, 271 - 279, 31.12.2017
Öz
Kaynakça
- Aydin M, Arslan E, Taspinar MS, Karadayi G, Agar G, 2016. Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.). Biotechnology and Biotechnological Equipment, 30: 1082-1089.
- Balague C, Sturtz N, Duffard R, Evangelista de Duffard AM, 2001. Effect of 2,4- dichlorophenoxyacetic acid herbicide on Escherichia coli growth, chemical composition, and cellular envelope. Environmental Toxicology, 16: 43–53.
- Correia S, Lopes ML, Canhoto JM, 2011. Somatic embryogenesis induction system for cloning an adult Cyphomandra betacea (Cav.) Sendt. (tamarillo). Trees, 25: 1009-1020.
- D’Agostino L, Di Pietro M, Di Luccia A, 2005. Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation. FEBS Journal, 272: 3777–3787.
- Duchnowicz P, Koter M, 2003. Damage to the erythrocyte membrane caused by chlorophenoxyacetic herbicides. Cellular and Molecular Biology Letters, 8: 25–30.
- Feng SJ, Liu XS, Tao H, Tan SK, Chu SS, Oono Y, Zhang XD, Chen J, Yang ZM, 2016. Variation of DNA methylation patterns associated with gene expression in rice (Oryza sativa) exposed to cadmium. Plant Cell and Environment, 39: 2629-2649.
- Grativol C, Hemerly AS, Ferreira PCG, 2012. Genetic and epigenetic regulation of stress responses in natural plant populations. Biochimica et Biophysica Acta, 1819: 176–185.
- Khan AU, Di-Mascio P, Medeiros MHG, Wilson T, 1992. Spermine and spermidine protection of plasmid DNA against single-strand breaks induced by singlet oxygen. Proceedings of the National Academy of Science of the United States of America, 89: 11428-11430.
- Leljak-Levanic D, Bauer N, Mihaljevic S, Jelaska S, 2004. Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L. Plant Cell Reports, 23: 120–127.
- Miyamoto S, Kashiwagi K, Watanabe S, Igarashi K, 1993. Estimation of polyamine distribution and polyamine stimulation of protein synthesis in Escherichia coli. Archives of Biochemistry and Biophysics, 300: 63-68.
- Mohammed KB, Ma TH, 1999. Tradescantia-micronucleus and -stamen hair mutation assays on genotoxicity of the gaseous and liquid forms of pesticides. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 426: 193-199.
- Oakes DJ, Webster WS, Brown Woodman PDC, Ritchie HE, 2002. Testicular changes induced by chronic exposure to the herbicide formulation, Tordon 75D® (2,4-dichlorophenoxyacetic acid and picloram) in rats. Reproductive Toxicology, 16: 281-289.
- Olea-Popelka F, Mclean DM, Horsman J, Almquist K, Bramdle JE, Hall C, 2005. Increasing expression of an anti-picloram single-chain variable fragment (ScFv) antibody and resistance to picloram in transgenic tobacco (Nicotiana tabacum). Journal of Agricultural and Food Chemistry, 53: 6683-6690.
- Ruiz-Herrera J, Ruiz-Medrano R, Dominguez A, 1995. Selective inhibition of cytosine DNA methylases by polyamines. FEBS Letters, 357: 192-196.
- Salvi ND, George L, Eapen S, 2001. Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell, Tissue and Organ Culture, 66: 113-119.
- Sawamura S, Jackson WT, 1968. Cytological studies in vivo of picloram, pyriclor, trifluralin, 2,3,6-TBA, 2,3,5,6-TBA and nitralin. Cytologia, 33: 545–554.
- Sung S, Amasino RM, 2004. Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3. Nature, 427: 159-164.
- Taspinar MS, Agar G, Yildirim N, Sunar S, Aksakal O, Bozari S, 2009. Evaluation of selenium effect on cadmium genotoxicity in Vicia faba using RAPD. Journal of Food, Agriculture and Environment, 7: 857-860.
- Temel A, Gozukirmizi N, 2013. Analysis of retrotransposition and DNA metyhlation in barley callus culture. Acta Biologica Hungarica, 64: 86-95.
- Wang W, Qiao Q, Sun F, Wang Y, Xu D, Li Z, Fu B, 2016. Genome-wide differences in DNA methylation changes in two contrasting rice genotypes in response to drought conditions. Frontiers in Plant Science, 7: 1675.
- Yildirim N, Agar G, Taspinar MS, Turan M, Aydin M, Arslan E, 2014. Protective role of humic acids against dicamba-induced genotoxicity and DNA methylation in Phaseolus vulgaris L. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 64: 141-148.
- Zeid IM, Shedeed ZA, 2006. Response of alfalfa to putrescine treatment under drought stress. Biologia Plantarum, 50: 635-640.
Toplam 22 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Mühendislik |
| Bölüm | Tarımsal Biyoteknoloji / Agricultural Biotechnology |
| Yazarlar | |
| Yayımlanma Tarihi | 31 Aralık 2017 |
| Gönderilme Tarihi | 28 Nisan 2017 |
| Kabul Tarihi | 3 Ağustos 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 7 Sayı: 4 |