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Genetic analysis of Carasobarbus Karaman, 1971 (Actinopterygii Cyprinidae) in Anatolia

Yıl 2020, Cilt: 5 Sayı: 4, 520 - 526, 31.12.2020
https://doi.org/10.35229/jaes.796051

Öz

We sequenced the complete mitochondrial cytochrome b gene (1141 bp) of 70 individuals from ten populations of three Carasobarbus species in Anatolia. Totally eight haplotypes were identified. The intraspecies genetic distance ranged from 0.00% to 0.21%, while it varied from 2.6% to 9.0% for interspecies. Except for Gaziantep samples with low sample size, high haplotype diversity (Hd= 0,590-0,833) and low nucleotide diversity (Pi= 0,05-0,65) values can be explained by the presence of small populations sensitive to genetic drift and founder effects. Phylogenetic analyses constructed with neihgbour joining, maximum likelihood and maximum parsimony generated similar topologies supported by high bootstrap values. Phylogenetic tree topologies showed that the C. apoensis haplotype was located in the C. luteus species. Therefore, the validity of species status of C. apoensis should be checked morphologically. On the other hand, since the Kahta population in C. luteus has a remarkably high genetic diversity, it must be re-evaluated morphologically. The tectonic uplift of the Anatolian Plateau between the African and European plates during the Pliocene period may have probably prevented the presence of Carasobarbus in the west of the Anatolian diagonal.

Destekleyen Kurum

Recep Tayyip Erdoğan Üniversitesi

Proje Numarası

2015.53008.103.01.01

Teşekkür

This work was funded by the scientific research funds of Recep Tayyip Erdogan University (RTEÜ BAP, Project No: 2015.53008.103.01.01).

Kaynakça

  • Abdoli, A. (2000). The Inland Water Fishes of Iran. Iranian Museum of Nature and Wildlife, Tehran. 378 pp. In Farsi.
  • Anderson, S., Bankler, A.T. & Barrel, B.G. (1981). Sequence and organization of the human mitochondrial genome. Nature, 290, 457-465.
  • Ansell, S.W., Stenlien, H.K., Grundmann, M., Russell, S.J., Koch, M.A., Schneider, H. & Vogel, J.C. (2011). The importance of Anatolian mountains as the cradle of global diversity in Arabis alpina, a key arctic – alpine species. Annals of Botany, 108, 241–252.
  • Aranishi, F., Okimoto, T. & Izumi, S. (2005). Identification of gadoid species (pisces, Gadidae) by PCR‐RFLP analysis. Journal of Applied Genetics, 46, 69–73.
  • Borkenhagen, K. & Krupp, F. (2013). Taxonomic revision of the genus Carasobarbus Karaman, 1971 (Actinopterygii, Cyprinidae). ZooKeys, 339, 1–53.
  • Borkenhagen, K. (2005). Systematik und Zoogeographie der “großschuppigen Barben” des Vorderen Orients. ChristianAlbrechts-Universität, Kiel
  • Borkenhagen, K. (2017). Molecular phylogeny of the tribe Torini Karaman, 1971 (Actinopterygii: Cypriniformes) from the Middle East and North Africa. Zootaxa, 4236, 291–301.
  • Borkenhagen, K., Esmaeili, HR., Mohsenzadeh S. & Shahryari, F.G.A. (2011). The molecular systematics of the Carasobarbus species from Iran and adjacent areas, with comments on Carasobarbus albus (Heckel, 1843). Environmental Biology of Fishes, 91, 327–335. Doi: 10.1007/s10641-011-9787-1.
  • Briolay, J., Galtier, N., Brito, R.M. & Bouvet, Y. (1998). Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences. Molecular Phylogenetics and Evolution, 9, 100–108. Doi: 10.1006/mpev.1997.0441.
  • Cantatore, P., Roberti, M., Pesole, G., Ludovico, A., Milella, F., Gadaleta, M. N. & Saccone, C. (1994). Evolutionary analysis of cytochrome b sequences in some perciformes evidence for a slower rate of evolution than in mammals. Journal of Molecular Evolution, 39, 589–597.
  • Durand, J.D., Tsigenopoulos, C.S., Ünlü, E. & Berrebi, P. (2002). Phylogeny and biogeography of the family Cyprinidae in the Middle East inferred from cytochrome b DNA – Evolutionary significance of this region. Molecular Phylogenetics and Evolution, 22, 91–100. Doi: 10.1006/mpev.2001.1040.
  • Esmaeili, H.R., Teimory, A., Hosseini, F. & Gholami, Z. (2006). Range extension report for Barbus sublimus Coad and Najafpoor, 1997 (Actinopterygii Cyprinidae) along with its sympatric species in southwest Iran. Iranian Journal of Animal Biosystematics, 2, 19–24.
  • Fairbridge, R., Erol, O., Karaca, M. & Yilmaz, Y. (1997). Background to Mid-Holocene climatic change in Anatolia and adjacent regions. In Dalfes, H.N., Kukla, G., Weiss, H. (Eds.), Third Millennium BC Climate Change and Old World CollapseNATO ASI series l 149. Springer, Berlin, 595–609.
  • Felsenstein, J. (1981). Evolutionary trees from DNA sequences a maximum likelihood approach. Journal of Molecular Evolution, 7, 368-376.
  • Froese, R& Pauly, D. (2015). Fishbase. World Wide Web Electronic Publication. Avaliable http//www.fishbase.org (May 30, 2019).
  • Gökçek, C.K., Mazlum, Y. & Akyurt, I. (2008). Effect of feeding frequency on the growth and survival of himri barbel Barbus luteus (Heckel, 1843), fry under laboratory conditions. Pakistan Journal of Nutrition, 7, 66-69.
  • Guindon, S. & Gascuel, O. (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52(5), 696 –704.
  • Gür, H. (2016). The Anatolian diagonal revisited testing the ecological basis of a biogeographic boundary. Zoology in the Middle East, 623, 189-199.
  • Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95- 98.
  • Karaman, M.S. (1971). Süßwasserfische der Türkei. 8. Teil: Revision der Barben Europas, Vorderasiens und Nordafrikas. Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 67, 175–254.
  • Kaya, C., Turan, D. & Ünlü, E. (2016). The latest status and distribution of fishes in upper tigris river and two new records for Turkish freshwaters. Turkish Journal of Fisheries and Aquatic Sciences, 16, 545-562.
  • Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
  • Kumar, S., Stecher, G. Li. M., Knyaz, C. & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology, 35, 1547–1549.
  • Kuru, M. (1979). The freshwater fish of South-Eastern Turkey-2 (euphrates-tigris system). Hacettepe Bulletin of Natural Sciences and Engineering, 7, 105-114.
  • Librado, P. & Rozas, J. (2009). DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451-1452.
  • Liu, Z.J. & Cordes, J.F. (2004). DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238, 1–37.
  • Parmaksız, A. & Eskici, H.K. (2018). Genetic variation of yellow barbell (Carasobarbus luteus Heckel, 1843) from four populatıons using mitochondrial DNA COI gene sequences. Applied Ecology and Environmental Research, 16, 1673-1682.
  • Phillips, J.D., Gillis, D.J. & Hanner, R.H. (2019). Incomplete estimates of genetic diversity within species Implications for DNA barcoding. Ecology and Evolution, 9, 2996–3010.
  • Posada, D. (2008). jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25(7), 1253–1256.
  • Sauvage, H.E. (1882). Catalogue des poissons recueillis par M. E. Chantre pendant son voyage en Syrie, Haute-Mésopotamie, Kurdistan et Caucase. Bulletin de la Société philomathique de Paris, 6, 163–168.
  • Swofford, D.L. (2003). PAUP. Phylogenetic Analysis Using Parsimony. Version 4. Sinauer Associates, Sunderland, Massachusetts.
  • Tang, Q., Liu H., Mayden, R. & Xiong, B. (2006). Comparison of evolutionary rates in the mitochondrial DNA cytochrome b gene and control region and their implications for phylogeny of the Cobitoidea (Teleostei Cypriniformes). Molecular Phylogenetics and Evolution, 39, 347–357.
  • Ünlü, E. (1991). Investigation on the biological characteristics of Capoeta trutta (Heckel, 1843) living in Tigris River. Turkish Journal Of Zoology, 15, 12-38.
  • Wang, J., Wu, X. Y., Chen, Z., Yue, Z., Ma, W., Chen, S. Y., Xiao, H., Murphy, R. W., Zhang, Y. P., Zan, R. G. & Luo, J. (2013). Molecular phylogeny of European and African Barbus and their West Asian relatives in the Cyprininae (Teleostei Cypriniformes) and orogenesis of the Qinghai-Tibetan Plateau. Chinese Science Bulletin, 58, 3738–3746.
  • Wright, S. (1943). Isolation by distance. Genetics, 28, 114–138.

Anadolu’daki Carasobarbus Karaman, 1971 (Actinopterygii Cyprinidae) genusunun genetik analizi

Yıl 2020, Cilt: 5 Sayı: 4, 520 - 526, 31.12.2020
https://doi.org/10.35229/jaes.796051

Öz

Anadolu'daki üç Carasobarbus türünün 10 populasyonundan 70 bireyin mitokondri sitokrom b geninin (1141 bp) DNA dizin analizi yapılmıştır. Toplamda sekiz haplotip tanımlandı. Sitokrom b veri analizi kullanılarak, tür içi genetik mesafenin % 0,00 ile % 0,21 arasında, türler arası için ise % 2,6 ile% 9,0 arasında değiştiği belirlenmiştir. Yeterli örneklem büyüklüğüne sahip olmayan Gaziantep örnekleri hariç yüksek haplotip çeşitlilik (Hd= 0,590-0,833) ve düşük nükleotit çeşitlilik (Pi= 0,05-0,65) değerleri, genetik sürüklenme ve kurucu etkilere karşı duyarlı olan küçük populasyonların varlığı ile açıklanabilir. Komşu birleştirme, maksimum tutumluluk ve maksimum olasılık metotları yoluyla oluşturulan filogenetik analizler yüksek güvenirlilik değerleri ile desteklenen benzer topolojiler üretmiştir. Filogenetik ağaç topolojileri gösterdi ki C. apoensis haplotipleri C. luteus haplotipleri içinde konumlandığı için türün geçerliliği morfolojik olarak kontrol edilmelidir. Diğer taraftan, C. luteus içindeki Kahta populasyonu dikkate değer düzeyde yüksek genetik çeşitliğe sahip olduğundan morfolojik olarak mutlaka yeniden değerlendirilmelidir. Pliyosen döneminde Afrika ve Avrupa levhaları arasındaki çarpışmadan kaynaklanan Anadolu Platosu’nun tektonik yükselişi muhtemelen Anadolu köşegeninin batısında Carasobarbus’un varlığını engellemiştir.

Proje Numarası

2015.53008.103.01.01

Kaynakça

  • Abdoli, A. (2000). The Inland Water Fishes of Iran. Iranian Museum of Nature and Wildlife, Tehran. 378 pp. In Farsi.
  • Anderson, S., Bankler, A.T. & Barrel, B.G. (1981). Sequence and organization of the human mitochondrial genome. Nature, 290, 457-465.
  • Ansell, S.W., Stenlien, H.K., Grundmann, M., Russell, S.J., Koch, M.A., Schneider, H. & Vogel, J.C. (2011). The importance of Anatolian mountains as the cradle of global diversity in Arabis alpina, a key arctic – alpine species. Annals of Botany, 108, 241–252.
  • Aranishi, F., Okimoto, T. & Izumi, S. (2005). Identification of gadoid species (pisces, Gadidae) by PCR‐RFLP analysis. Journal of Applied Genetics, 46, 69–73.
  • Borkenhagen, K. & Krupp, F. (2013). Taxonomic revision of the genus Carasobarbus Karaman, 1971 (Actinopterygii, Cyprinidae). ZooKeys, 339, 1–53.
  • Borkenhagen, K. (2005). Systematik und Zoogeographie der “großschuppigen Barben” des Vorderen Orients. ChristianAlbrechts-Universität, Kiel
  • Borkenhagen, K. (2017). Molecular phylogeny of the tribe Torini Karaman, 1971 (Actinopterygii: Cypriniformes) from the Middle East and North Africa. Zootaxa, 4236, 291–301.
  • Borkenhagen, K., Esmaeili, HR., Mohsenzadeh S. & Shahryari, F.G.A. (2011). The molecular systematics of the Carasobarbus species from Iran and adjacent areas, with comments on Carasobarbus albus (Heckel, 1843). Environmental Biology of Fishes, 91, 327–335. Doi: 10.1007/s10641-011-9787-1.
  • Briolay, J., Galtier, N., Brito, R.M. & Bouvet, Y. (1998). Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences. Molecular Phylogenetics and Evolution, 9, 100–108. Doi: 10.1006/mpev.1997.0441.
  • Cantatore, P., Roberti, M., Pesole, G., Ludovico, A., Milella, F., Gadaleta, M. N. & Saccone, C. (1994). Evolutionary analysis of cytochrome b sequences in some perciformes evidence for a slower rate of evolution than in mammals. Journal of Molecular Evolution, 39, 589–597.
  • Durand, J.D., Tsigenopoulos, C.S., Ünlü, E. & Berrebi, P. (2002). Phylogeny and biogeography of the family Cyprinidae in the Middle East inferred from cytochrome b DNA – Evolutionary significance of this region. Molecular Phylogenetics and Evolution, 22, 91–100. Doi: 10.1006/mpev.2001.1040.
  • Esmaeili, H.R., Teimory, A., Hosseini, F. & Gholami, Z. (2006). Range extension report for Barbus sublimus Coad and Najafpoor, 1997 (Actinopterygii Cyprinidae) along with its sympatric species in southwest Iran. Iranian Journal of Animal Biosystematics, 2, 19–24.
  • Fairbridge, R., Erol, O., Karaca, M. & Yilmaz, Y. (1997). Background to Mid-Holocene climatic change in Anatolia and adjacent regions. In Dalfes, H.N., Kukla, G., Weiss, H. (Eds.), Third Millennium BC Climate Change and Old World CollapseNATO ASI series l 149. Springer, Berlin, 595–609.
  • Felsenstein, J. (1981). Evolutionary trees from DNA sequences a maximum likelihood approach. Journal of Molecular Evolution, 7, 368-376.
  • Froese, R& Pauly, D. (2015). Fishbase. World Wide Web Electronic Publication. Avaliable http//www.fishbase.org (May 30, 2019).
  • Gökçek, C.K., Mazlum, Y. & Akyurt, I. (2008). Effect of feeding frequency on the growth and survival of himri barbel Barbus luteus (Heckel, 1843), fry under laboratory conditions. Pakistan Journal of Nutrition, 7, 66-69.
  • Guindon, S. & Gascuel, O. (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52(5), 696 –704.
  • Gür, H. (2016). The Anatolian diagonal revisited testing the ecological basis of a biogeographic boundary. Zoology in the Middle East, 623, 189-199.
  • Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95- 98.
  • Karaman, M.S. (1971). Süßwasserfische der Türkei. 8. Teil: Revision der Barben Europas, Vorderasiens und Nordafrikas. Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 67, 175–254.
  • Kaya, C., Turan, D. & Ünlü, E. (2016). The latest status and distribution of fishes in upper tigris river and two new records for Turkish freshwaters. Turkish Journal of Fisheries and Aquatic Sciences, 16, 545-562.
  • Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
  • Kumar, S., Stecher, G. Li. M., Knyaz, C. & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology, 35, 1547–1549.
  • Kuru, M. (1979). The freshwater fish of South-Eastern Turkey-2 (euphrates-tigris system). Hacettepe Bulletin of Natural Sciences and Engineering, 7, 105-114.
  • Librado, P. & Rozas, J. (2009). DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451-1452.
  • Liu, Z.J. & Cordes, J.F. (2004). DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238, 1–37.
  • Parmaksız, A. & Eskici, H.K. (2018). Genetic variation of yellow barbell (Carasobarbus luteus Heckel, 1843) from four populatıons using mitochondrial DNA COI gene sequences. Applied Ecology and Environmental Research, 16, 1673-1682.
  • Phillips, J.D., Gillis, D.J. & Hanner, R.H. (2019). Incomplete estimates of genetic diversity within species Implications for DNA barcoding. Ecology and Evolution, 9, 2996–3010.
  • Posada, D. (2008). jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25(7), 1253–1256.
  • Sauvage, H.E. (1882). Catalogue des poissons recueillis par M. E. Chantre pendant son voyage en Syrie, Haute-Mésopotamie, Kurdistan et Caucase. Bulletin de la Société philomathique de Paris, 6, 163–168.
  • Swofford, D.L. (2003). PAUP. Phylogenetic Analysis Using Parsimony. Version 4. Sinauer Associates, Sunderland, Massachusetts.
  • Tang, Q., Liu H., Mayden, R. & Xiong, B. (2006). Comparison of evolutionary rates in the mitochondrial DNA cytochrome b gene and control region and their implications for phylogeny of the Cobitoidea (Teleostei Cypriniformes). Molecular Phylogenetics and Evolution, 39, 347–357.
  • Ünlü, E. (1991). Investigation on the biological characteristics of Capoeta trutta (Heckel, 1843) living in Tigris River. Turkish Journal Of Zoology, 15, 12-38.
  • Wang, J., Wu, X. Y., Chen, Z., Yue, Z., Ma, W., Chen, S. Y., Xiao, H., Murphy, R. W., Zhang, Y. P., Zan, R. G. & Luo, J. (2013). Molecular phylogeny of European and African Barbus and their West Asian relatives in the Cyprininae (Teleostei Cypriniformes) and orogenesis of the Qinghai-Tibetan Plateau. Chinese Science Bulletin, 58, 3738–3746.
  • Wright, S. (1943). Isolation by distance. Genetics, 28, 114–138.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Didem Bahadır Bu kişi benim 0000-0001-6258-7319

İsmail Aksu 0000-0002-2104-9888

Yusuf Bektaş 0000-0002-8367-9746

Proje Numarası 2015.53008.103.01.01
Yayımlanma Tarihi 31 Aralık 2020
Gönderilme Tarihi 17 Eylül 2020
Kabul Tarihi 3 Kasım 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 5 Sayı: 4

Kaynak Göster

APA Bahadır, D., Aksu, İ., & Bektaş, Y. (2020). Genetic analysis of Carasobarbus Karaman, 1971 (Actinopterygii Cyprinidae) in Anatolia. Journal of Anatolian Environmental and Animal Sciences, 5(4), 520-526. https://doi.org/10.35229/jaes.796051


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JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS