Investigation into the Usability of the Maackia amurensis Lectin in Bacterial Diagnosis with the Help of Transmission Electron Microscope
Öz
Objective: Glycoconjugates are known to play a crucial role in the attachment-recognition relationship of prokaryotic cells, particularly bacteria. Therefore, understanding the function and properties of these glycoconjugates is of great importance.
Materials and Methods: While various methods have provided significant information in determining the chemical structures and functions of sialic acids, histological methods have remained essential in determining their function and location in living organisms. The discovery of lectins with unique marking ability based on the bond structures of sialic acids and the development of antibodies, which can be microscopically distinguished by adding colloidal silver and gold particles, were significant milestones. In the 1990s, methods were developed to specifically mark the types and locations of sialic acids using immunocytological, histological, and fluorescence microscopy methods with fluorescently labeled lectins and antibodies. These methods still remain valid and important today. Using this information, it is possible to create smart drugs and biomarkers specific to bacteria.
Results: In this study, the unique connections of gold-labeled lectins with different bacteria were demonstrated with the help of transmission electron microscopy.
Conclusion: Our study supports the idea that labeled lectins could be used for rapid and precise bacterial diagnosis.
Anahtar Kelimeler
Sialic acid Bacteria Biomarkers Immunocytochemistry Microscopy
Kaynakça
- Schauer R, Kamerling JP. Exploration of the sialic acid world. Adv Carbohydr Chem Biochem. 2018;75:1-213. google scholar
- Alagesan K, Hoffmann M, Rapp E, et al. Glycoproteomics technologies in glycobiotechnology. Adv Biochem Eng Biotech. 2021;175:413-434. google scholar
- Sackstein R, Hoffmeister KM et al. Sialic acids in human health and disease. In Varki A, Cummings RD et al. editors. Essentials of Glycobiology. 4th ed. New York, N.Y.: Cold Spring Harbor Laboratory Press; 2022. Chapter 46. google scholar
- Lanctot PM, Gage FH, Varki AP. The glycans of stem cells. Curr Opin Chem Biol. 2007;11:373-380. google scholar
- Varki A, Cummings RD, Esko JD, et al. Essentials of Glycobiology. 4th ed. New York, N.Y.: Cold Spring Harbor Laboratory Press; 2022. google scholar
- Song Y, Zhang F, Linhardt RJ. Glycosaminoglycans. Adv Exp Med Biol. 2021;1325:103-116. google scholar
- Mater Y. The cloning, expression and structure-function relationships of the Escherichia coli sialyltransferase gene. Ege University, Graduate School of Natural and Applied Science, PhD Thesis, 2004. google scholar
- Silva M, Luísa S. Lectin biosensors in cancer glycan biomarker detection. Adv Clin Chem. 2019;93:1-61. google scholar
- Mater Y, Ozdas S. The analysis of surface saccharide profiles through fluorescein-labelled lectins in a rat pancreatic tissue with established metabolic syndrome model. Turkish J Biochem. 2019;44:98-104. google scholar
- Demircan G, Mater Y. Effects of fluorescent marked Maackia amurensis-lectin-1 and wheat germ agglutinin on the cell surface glycan profiles in two different breast cancer cell lines. J IstFaculty Med. 2019;82:89-95. google scholar
- Kamerling JP, Gerwig GJ. Strategies for the structural analysis of carbohydrates. Comprehensive Glycoscience. 2007;2:1-68. doi:10.1016/B978-044451967-2/00032-5. google scholar
- Biomerieux Diagnostics website. https://www.biomerieuxdiagnostics. com/vitek-2-advanced-expert-system. Accessed October 20, 2023. google scholar
- Madigan MT, Bender KS, Buckley DH Brock Biology of Microorganisms. 16th ed. New York, N.Y.: Pearson Education Limited; 2021. google scholar
- Hayat MA. Colloidal Gold Principles, Methods and Applications. San Diago, California: Academic Press; 1989. google scholar
- Jones CJP, AllenWRT,Wilsher S. A lectin histochemical study to detect variation in glycosylation at the feto-maternal interface in three interbreeding equine species. Placenta. 2017;58:115-121. google scholar
- Hu M, Lan Y, Lu A, Ma X, Zhang L. Glycan-based biomarkers for diagnosis of cancers and other diseases: Past, present, and future. Prog Mol Biol Transl Sci. 2019;162:1-24. google scholar
- Zhang XL, Qu H. The role of glycosylation in infectious diseases. Adv Exp Med Biol. 2021;1325:219-237. google scholar
- Fujitani N, Furukawa J, Araki K, et al. Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers. Proc Natl Acad Sci USA. 2013;110(6):2105-2110. google scholar
- Costa AF, Campos D, Reis CA, et al. Targeting glycosylation: A new road for cancer drug discovery. Trends Cancer. 2020;6(9):757-766. google scholar
Öz
Kaynakça
- Schauer R, Kamerling JP. Exploration of the sialic acid world. Adv Carbohydr Chem Biochem. 2018;75:1-213. google scholar
- Alagesan K, Hoffmann M, Rapp E, et al. Glycoproteomics technologies in glycobiotechnology. Adv Biochem Eng Biotech. 2021;175:413-434. google scholar
- Sackstein R, Hoffmeister KM et al. Sialic acids in human health and disease. In Varki A, Cummings RD et al. editors. Essentials of Glycobiology. 4th ed. New York, N.Y.: Cold Spring Harbor Laboratory Press; 2022. Chapter 46. google scholar
- Lanctot PM, Gage FH, Varki AP. The glycans of stem cells. Curr Opin Chem Biol. 2007;11:373-380. google scholar
- Varki A, Cummings RD, Esko JD, et al. Essentials of Glycobiology. 4th ed. New York, N.Y.: Cold Spring Harbor Laboratory Press; 2022. google scholar
- Song Y, Zhang F, Linhardt RJ. Glycosaminoglycans. Adv Exp Med Biol. 2021;1325:103-116. google scholar
- Mater Y. The cloning, expression and structure-function relationships of the Escherichia coli sialyltransferase gene. Ege University, Graduate School of Natural and Applied Science, PhD Thesis, 2004. google scholar
- Silva M, Luísa S. Lectin biosensors in cancer glycan biomarker detection. Adv Clin Chem. 2019;93:1-61. google scholar
- Mater Y, Ozdas S. The analysis of surface saccharide profiles through fluorescein-labelled lectins in a rat pancreatic tissue with established metabolic syndrome model. Turkish J Biochem. 2019;44:98-104. google scholar
- Demircan G, Mater Y. Effects of fluorescent marked Maackia amurensis-lectin-1 and wheat germ agglutinin on the cell surface glycan profiles in two different breast cancer cell lines. J IstFaculty Med. 2019;82:89-95. google scholar
- Kamerling JP, Gerwig GJ. Strategies for the structural analysis of carbohydrates. Comprehensive Glycoscience. 2007;2:1-68. doi:10.1016/B978-044451967-2/00032-5. google scholar
- Biomerieux Diagnostics website. https://www.biomerieuxdiagnostics. com/vitek-2-advanced-expert-system. Accessed October 20, 2023. google scholar
- Madigan MT, Bender KS, Buckley DH Brock Biology of Microorganisms. 16th ed. New York, N.Y.: Pearson Education Limited; 2021. google scholar
- Hayat MA. Colloidal Gold Principles, Methods and Applications. San Diago, California: Academic Press; 1989. google scholar
- Jones CJP, AllenWRT,Wilsher S. A lectin histochemical study to detect variation in glycosylation at the feto-maternal interface in three interbreeding equine species. Placenta. 2017;58:115-121. google scholar
- Hu M, Lan Y, Lu A, Ma X, Zhang L. Glycan-based biomarkers for diagnosis of cancers and other diseases: Past, present, and future. Prog Mol Biol Transl Sci. 2019;162:1-24. google scholar
- Zhang XL, Qu H. The role of glycosylation in infectious diseases. Adv Exp Med Biol. 2021;1325:219-237. google scholar
- Fujitani N, Furukawa J, Araki K, et al. Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers. Proc Natl Acad Sci USA. 2013;110(6):2105-2110. google scholar
- Costa AF, Campos D, Reis CA, et al. Targeting glycosylation: A new road for cancer drug discovery. Trends Cancer. 2020;6(9):757-766. google scholar
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 21 Aralık 2023 |
| Gönderilme Tarihi | 21 Temmuz 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 82 Sayı: 2 |
