Farklı bitki büyüme düzenleyicilerle stimule edilen limon otu (Melissa officinalis L.) kallus kültürlerindeki toplam fenolik bileşikler ve flavanoidlerin belirlenmesi
Öz
Limon otu (Melissa
officinalis L.), tıbbi ve aromatik bir bitki olarak kullanılan türlerden
olup, iç piyasada pazarlanmakta ve ihracatı yapılmaktadır. Limon otu eczacılık,
parfümeri, kozmetik ve gıda sanayinde çok sayıda kullanım alanına sahiptir. M. officinalis’in önemi içerisinde
bulunan uçucu yağlar olan citronelal ve citral ’den kaynaklanmaktadır. Bu iki
bileşenin yanında daha düşük oranlarda linalool, geraniol, ά-pinen, terpinen
gibi bileşikler içermektedir. Kallus kültürleri ve hücre süspansiyon kültürleri
tıbbi ve aromatik bitkilerin in vitro çoğaltım yollarıdır. Bu sayede aynı
kalitede ve yüksek miktarda bitki sekonder metabolitlerini elde etmek
mümkündür. Bu çalışmada in vitro olarak üretilen kallusların toplam fenolik
madde ve flavanoid miktarları belirlenmiştir. Eksplant kaynağı olarak M. Officinalis nodları kullanılmıştır.
Nodlar Murashige Skoog besiyerinde kallus oluşumu için farklı bitki büyüme
düzenleyici kombinasyonları ile teşvik edilmiştir. En yüksek fenolik madde
miktarı 1523 mg/g ile 1.5 mg/L 2,4-D + 0.5 mg/L BAP ile teşvik edilen kallusta
tespit edilmiştir. 1.356 mg/g ile en düşük fenolik madde ise 2 mg/L 2,4-D + 0.5
mg/L BAP içeren kültür ortamındaki kalluslarda görülmüştür. Flavanoid
miktarının en yüksek olduğu (4.392
mg/g) kallusun bulunduğu kültür
ortamında ise bitki büyüme düzenleyicisi olarak 2 mg/L 2,4-D + 1 mg/L PİC + 0.5
mg/L KİN bulunmaktadır.
Anahtar Kelimeler
flavanoid kallus Melissa officinalis L. fenolik bileşik antioksidan
Kaynakça
- Allahverdiyev, A., Duran, N., Ozguven, M. and Koltas, S. 2004. Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2. Phytomedicine, 11(7-8), 657-661.
- Ahmad, N., Rab, A. 2016. Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert). Journal of Photochemistry and Photobiology B: Biology, 154, 51-56.
- Argyropoulos, D., Müller, J. 2014. Changes of essential oil content and composition during convective drying of lemon balm (Melissa officinalis L.). Industrial Crops and Products. 52. 118-224.
- Arslan, N., Gürbüz, B., Gümüşçü, A., 2002. Tıbbi Bitkiler İsim Kılavuzu. Ankara Üniversitesi, Ziraat Fakültesi Yayınları No.1530, p:180.
- Awad, R., Muhammad, A., Durst, T., Trudeau, V. T., Arnason, J. T. 2009. Bioassay‐guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity. Phytothrapy Research, Volume23, Issue8, 1075-1081.
- Basta, A., Tzakou, O. and Couladis, M. 2005. Composition of the leaves essential oil of Melissa officinalis sl from Greece. Flavour and fragrance journal, 20(6), 642-644.
- Beloued A. 2009. Plantes médicinales d’Algérie. Alger: Office des Publications Universitaires, (p 134).
- Birdane YO, Buyukokuroglu ME, Birdane FM, Cemek M, Yavuz H.,2007. Anti-inflammatory and antinociceptive effects of Melissa Officinalis L. in rodents.Rev Méd Vét ,158:75-81.
- Capecka, E., Mareczek, A. and Leja, M. 2005. Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food chemistry, 93(2), 223-226.Chung MJ, Cho SY, Bhuiyan MJH, Kim KH, Lee SJ.,2010.Anti-diabetic effects of lemon balm (Melissa officcinalis) essential oil on glucose and lipid regulating enzymes in type 2 diabetic mice. Brit J Nutr.104: 180-8.
- El-Beltagi, H. S., Ahmed, O. K. and El-Desouky, W. 2011. Effect of low doses γ-irradiation on oxidative stress and secondary metabolites production of rosemary (Rosmarinus officinalis L.) callus culture. Radiation Physics and Chemistry, 80(9), 968-976.
- Farahani, H. A., Valadabadi, S. A., Daneshian, J. and Khalvati, M. A. 2009. Evaluation changing of essential oil of balm (Melissa officinalis L.) under water deficit stress conditions. Journal of Medicinal Plants Research, 3(5), 329-333.
- Giri, L., Dhyani, P., Rawat, S., Bhatt, I. D., Nandi, S. K., Rawal, R. S. and Pande, V. 2012. In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: a rare Himalayan medicinal orchid. Industrial Crops and Products, 39, 1-6.
- Hussain, AI, Anwar F, Nigam PS, Saker SD, Moore JE, Rao JR et al. 2011. Antimicrobial activity of some Lamiaceae essential oils using resazurin as an indicator of cell growth. LWT 44:1199-206.
- Ivanova, D., Gerova, D., Chervenkov, T. and Yankova, T. 2005. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. Journal of ethnopharmacology, 96(1-2), 145-150.
- Kahkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S. and Heinonen, M. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of agricultural and food chemistry, 47(10), 3954-3962.
- Lamaison, J. L., Carnat, A. and Petitjean-Freytet, C. 1990. Tannin content and inhibiting activity of elastase in Rosaceae. In Annales pharmaceutiques francaises (Vol. 48, No. 6, pp. 335-340).
- Mabrouki, H., Duarte, C. M. M. and Akretche, D. E. 2017. Estimation of Total Phenolic Contents and In Vitro Antioxidant and Antimicrobial Activities of Various Solvent Extracts of Melissa officinalis L. Arabian Journal for Science and Engineering, 1-9.
- Moradkhani, H., Sargsyan, E., Bibak, H., Naseri, B., Sadat-Hosseini, M., Fayazi-Barjin, A. and Meftahizade, H. 2010. Melissa officinalis L., a valuable medicine plant. Journal of Medicinal Plants Research Vol. 4(25), pp. 2753-2759.
- Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497.
- Saglam, C., Atakisi, I., Turhan, H., Kaba, S., Arslanoglu, F. and Onemli, F. 2004. Effect of propagation method, plant density, and age on lemon balm (Melissa officinalis) herb and oil yield.
- Sarı, A. O. and Ceylan, A. 2002. Yield characteristics and essential oil composition of lemon balm (Melissa officinalis L.) grown in the Aegean region of Turkey. Turkish Journal of Agriculture and Forestry, 26(4), 217-224.
- Singleton, V. L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158.
- Spiridon I, Colceru S, Anghel N, Teaca C.A, Bodirlau R, Armatu A. 2011. Antioxidant capacity and total phenolic contents of oregano (Origanum vulgare), lavender (Lavandula angustifolia) and lemon balm (Melissa officinalis) from Romania, Nat Prod Res., Oct;25(17):1657-61.
- Vitullo M, Ripabelli I, Fanelli I, Tamburro M, Delfine S, Sammarco L. 2011. Microbiological and toxicological quality of dried herbs, Lett Appl Microbiol. 52:573-80.
Determination of total phenolic compounds and flavanoids in callus cultures of lemon grass (Melissa Officinalis L.) stimulated with different plant growth regulators
Öz
Lemon grass (Melissa officinalis L.) is a species
used as a medicinal and aromatic plant, marketed on the domestic market and
also exported. Lemon grass has many uses in the pharmaceutical, perfumery,
cosmetics and food industries. The prominence of M. officinalis is due to the
essential oils found in citronelal and citral. In addition to these two
components, compounds such as linalool, geraniol, ά-pinene and terpinene are
contained at a lower rate. Callus cultures and cell suspension cultures are in
vitro propagation pathways for medical and aromatic plants. It is possible to
obtain metabolites of plant secondary in the same quality and in high quantity.
In this study, total phenolic substances and flavanoid amounts of calli
produced in vitro were determined. M. officinalis nodes were used as explant
source. Nodules were promoted with different plant growth regulator
combinations for callus formation in Murashige Skoog medium. The highest
phenolic substance content was determined from 1523 mg / g to 1.5 mg / L 2,4-D
+ 0.5 mg / L BAP-induced callus. 1.356 mg / g and the lowest phenolic substance
was found in cultured calli containing 2 mg / L 2,4-D + 0.5 mg / L BAP. In the
culture medium with the highest amount of flavanoid (4.392 mg / g), there is a
plant growth regulator of 2 mg / L 2,4-D + 1 mg / L PIC + 0.5 mg / L KIN..
Anahtar Kelimeler
flavanoid Melissa officinalis L. phenolic compound antioxidant callus
Kaynakça
- Allahverdiyev, A., Duran, N., Ozguven, M. and Koltas, S. 2004. Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2. Phytomedicine, 11(7-8), 657-661.
- Ahmad, N., Rab, A. 2016. Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert). Journal of Photochemistry and Photobiology B: Biology, 154, 51-56.
- Argyropoulos, D., Müller, J. 2014. Changes of essential oil content and composition during convective drying of lemon balm (Melissa officinalis L.). Industrial Crops and Products. 52. 118-224.
- Arslan, N., Gürbüz, B., Gümüşçü, A., 2002. Tıbbi Bitkiler İsim Kılavuzu. Ankara Üniversitesi, Ziraat Fakültesi Yayınları No.1530, p:180.
- Awad, R., Muhammad, A., Durst, T., Trudeau, V. T., Arnason, J. T. 2009. Bioassay‐guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity. Phytothrapy Research, Volume23, Issue8, 1075-1081.
- Basta, A., Tzakou, O. and Couladis, M. 2005. Composition of the leaves essential oil of Melissa officinalis sl from Greece. Flavour and fragrance journal, 20(6), 642-644.
- Beloued A. 2009. Plantes médicinales d’Algérie. Alger: Office des Publications Universitaires, (p 134).
- Birdane YO, Buyukokuroglu ME, Birdane FM, Cemek M, Yavuz H.,2007. Anti-inflammatory and antinociceptive effects of Melissa Officinalis L. in rodents.Rev Méd Vét ,158:75-81.
- Capecka, E., Mareczek, A. and Leja, M. 2005. Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food chemistry, 93(2), 223-226.Chung MJ, Cho SY, Bhuiyan MJH, Kim KH, Lee SJ.,2010.Anti-diabetic effects of lemon balm (Melissa officcinalis) essential oil on glucose and lipid regulating enzymes in type 2 diabetic mice. Brit J Nutr.104: 180-8.
- El-Beltagi, H. S., Ahmed, O. K. and El-Desouky, W. 2011. Effect of low doses γ-irradiation on oxidative stress and secondary metabolites production of rosemary (Rosmarinus officinalis L.) callus culture. Radiation Physics and Chemistry, 80(9), 968-976.
- Farahani, H. A., Valadabadi, S. A., Daneshian, J. and Khalvati, M. A. 2009. Evaluation changing of essential oil of balm (Melissa officinalis L.) under water deficit stress conditions. Journal of Medicinal Plants Research, 3(5), 329-333.
- Giri, L., Dhyani, P., Rawat, S., Bhatt, I. D., Nandi, S. K., Rawal, R. S. and Pande, V. 2012. In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: a rare Himalayan medicinal orchid. Industrial Crops and Products, 39, 1-6.
- Hussain, AI, Anwar F, Nigam PS, Saker SD, Moore JE, Rao JR et al. 2011. Antimicrobial activity of some Lamiaceae essential oils using resazurin as an indicator of cell growth. LWT 44:1199-206.
- Ivanova, D., Gerova, D., Chervenkov, T. and Yankova, T. 2005. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. Journal of ethnopharmacology, 96(1-2), 145-150.
- Kahkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S. and Heinonen, M. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of agricultural and food chemistry, 47(10), 3954-3962.
- Lamaison, J. L., Carnat, A. and Petitjean-Freytet, C. 1990. Tannin content and inhibiting activity of elastase in Rosaceae. In Annales pharmaceutiques francaises (Vol. 48, No. 6, pp. 335-340).
- Mabrouki, H., Duarte, C. M. M. and Akretche, D. E. 2017. Estimation of Total Phenolic Contents and In Vitro Antioxidant and Antimicrobial Activities of Various Solvent Extracts of Melissa officinalis L. Arabian Journal for Science and Engineering, 1-9.
- Moradkhani, H., Sargsyan, E., Bibak, H., Naseri, B., Sadat-Hosseini, M., Fayazi-Barjin, A. and Meftahizade, H. 2010. Melissa officinalis L., a valuable medicine plant. Journal of Medicinal Plants Research Vol. 4(25), pp. 2753-2759.
- Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497.
- Saglam, C., Atakisi, I., Turhan, H., Kaba, S., Arslanoglu, F. and Onemli, F. 2004. Effect of propagation method, plant density, and age on lemon balm (Melissa officinalis) herb and oil yield.
- Sarı, A. O. and Ceylan, A. 2002. Yield characteristics and essential oil composition of lemon balm (Melissa officinalis L.) grown in the Aegean region of Turkey. Turkish Journal of Agriculture and Forestry, 26(4), 217-224.
- Singleton, V. L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158.
- Spiridon I, Colceru S, Anghel N, Teaca C.A, Bodirlau R, Armatu A. 2011. Antioxidant capacity and total phenolic contents of oregano (Origanum vulgare), lavender (Lavandula angustifolia) and lemon balm (Melissa officinalis) from Romania, Nat Prod Res., Oct;25(17):1657-61.
- Vitullo M, Ripabelli I, Fanelli I, Tamburro M, Delfine S, Sammarco L. 2011. Microbiological and toxicological quality of dried herbs, Lett Appl Microbiol. 52:573-80.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 15 Ağustos 2018 |
| Kabul Tarihi | 13 Ağustos 2018 |
| Yayımlandığı Sayı | Yıl 2018 Cilt: 1 Sayı: 1 |
Kaynak Göster
