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Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Yıl 2019, Cilt: 36 Sayı: 2, 109 - 114, 15.06.2019
https://doi.org/10.12714/egejfas.2019.36.2.02

Öz



In study this, the level of DNA damage in three populations of endemic Beyşehir frog (Pelophylax caralitanus) inhabiting on Lakes of Karamık, Eber and Beyşehir with different anthropogenic pollution was assessed by using the DNA Comet Assay technique. Frog erythrocytes cells were used for the analysis. Seven adult individuals were collected from each biotope containing minimum 100 cells were analysed. While the results showing a significant increase in the DNA comet index in the contaminated zone [(Eber Lake= Idc=0.22±0.015), (p<0.05)], the majority of the cells did not contain any DNA damage in the clean zone (Karamık Lake, Idc=0.08±0.001). The obtained data demonstrated t that the endemic Beyşehir frog could serve as a useful flag species in an indicator of anthropogenic impact on ecosystems.




Destekleyen Kurum

TÜBİTAK - Afyon Kocatepe University

Proje Numarası

KBAG 113Z139 - 16.FENED.07

Teşekkür

This work was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK), Grant No:KBAG 113Z139 and Afyon Kocatepe University Research Funds Grant No:16.FENED.07

Kaynakça

  • Arıkan, H. (1988). On a new form Rana ridibunda (Anura: Ranidae) from Turkey. Istanbul Üniversitesi Fen Fakültesi Mecmuası, İstanbul, 53, 81-87.
  • Ashraf, W. (2005). Accumulation of heavy metals in kidney and heart tissues of Epinephelus microdon fish from the Arabian Gulf, Environmental Monitoring and Assessment. 101, 311-316. DOI:10.1007/s10661-005-0298-4.
  • Budak, A., Tok, C.V. & Ayaz, D. (2000). On specimens of Rana ridibunda Pallas, 1771 (Anura: Ranidae) collected from Işıklı Lake (Çivril-Denizli). Turkish Journal of Zoology, 24, 135-137.
  • Cotelle, S. & Férard, J.F. (1999). Comet assay in genetic ecotoxicology, A review. Environmental and Molecular Mutagenesis, 34, 246-255. DOI:10.1002/(SICI)1098-2280.
  • Dautremepuits, C., Paris-Palacios, S., Betoulle, S. & Vernet G. (2004). Modulation in hepatic and head kidney parameters of Carp (Cyprinus carpio) induced by copper and chitosan. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 137, 325-333.
  • Devaux, A., Flammarion, P. & Bernardon, V. (1998). Monitoring of the chemical pollution of the river Rhonethrough measurement of DNA damage and cytochrome P-450IA induction in chub (Leuciscus cephalus). Marine Environmental Research, 46, 257-262. DOI:10.1016/S0141-1136(97)00105.
  • Dhawan, A., Bajpayee, M. & Parmar, D. (2009). Comet assay: a reliable tool for the assessment of DNA damage in different models. Cell Biology and Toxicology, 25, 5-32. DOI:10.1007/s10565-008-9072.
  • El-Zein, R.A., Hastings-Smith, D.A., Ammenheuser, M.M., Trinen-Moslen, M., Gulland, F.M. & Ward, J.B. (2006). Evaluation of two different biomarkers for use in the assessment of toxic chemical exposure in California sea lions (Zalophus californianus). Marine Pollution Bulletin, 52, 104-120. DOI:10.1016/j.marpolbul. 2005.10.007.
  • Erismis U.C. (2018). Age, size, and growth of the Turkish endemic frog Pelophylax caralitanus (Anura: Ranidae). The Anatomical Record, 301, 1224-1234.
  • Erismis, U.C. & Chinsamy, A. (2010). Ontogenetic changes in the epiphyseal cartilage of Rana (Pelophylax) caralitana (Anura: Ranidae). The Anatomical Record, 293(11), 1825-1837. DOI: 10.1002 /ar.21241.
  • Erismis, U.C., Cigerci, I.H. & Konuk, M. (2013). Evaluation of DNA damage in Eurasian Marsh Frogs (Pelophylax ridibundus) by Comet Assay for determination of possible pollution in the different lakes in Central Anatolia, Turkey. The Bulletin of Environmental Contamination and Toxicology, 90, 660-665. DOI:10.1007/s00128-013-0991.
  • Ferreira, C.M., Lombardi, J.V., Machado-Neto, J.G., Bueno-Guimarães, H.M., Soares S.R.C. & Saldiva, P.H.N. (2004). Effects of copper oxychloride in Rana catesheiana tadpoles: toxicological and bioaccumulative aspects. Bulletin of Environmental Contamination and Toxicology, 73, 465-470.
  • Gürkan, M., Hayretdağ, S., Yakın, B.Y. & Tok, C.V. (2012). A preliminary study on micronucleus analysis and nuclear abnormalities in Pelophylax ridibundus (Pallas, 1771) (Amphibia: Anura) specimens collected around Vize (Kırklareli) and Ida Mountains (Çanakkale, Turkey). Ege Journal of Fisheries and Aquatic Sciences, 29, 133-136. DOI: egejfas.2012.29.3.05.
  • Hartmann, M., Schneider, T.R., Pfeil, A., Heinrich, G., Lipscomb, W.N. & Braus, G.H. (2003). Evolution of feedback-inhibited beta/alpha barrel isoenzymes by gene duplication and a single mutation. Proceedings of the National Academy of Sciences of the United States of America, 100(3), 862-7. DOI:10.1073/pnas.0337566100.
  • Huang, D., Zhang, Y., Wang, Y., Xie, Z. & Ji, W. (2007). Assessment of the genotoxicityin toad Bufo raddei exposed to petrochemical contaminants in Lanzhou Region, China. Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 629, 81-8. DOI: 10.1016 /j.mrgentox.2007.01.007.
  • Ismail, M., Khan, Q., Ali, R., Ali, T. & Mobeen, A. (2014). Evaluation of the genotoxicity of chlorpyrifos in common indus valley toad, Bufo stomaticus using alkaline single-cell gel electrophoresis (Comet) assay. The Journal of Agricultural Science 5, 376-382
  • Jdeidi, T., Bilgin, C.C. & Kence, M. (2001). New localities extend the range of Rana bedriagae caralitana Arıkan, 1988 (Anura: Ranidae) further west and suggest specific status. Turkish Journal of Zoology, 25, 153-158.
  • Kalay, M. Ay, O. & Canil, M. (1999). Heavy metal concentration in fish tissues from the northeast Mediterean Sea. The Bulletin of Environmental Contamination and Toxicology, 63, 673-681. DOI: 10.1007/s001289901033.
  • Kaya, U., Çevik, I.E. & Erişmiş, U.C. (2002). New distributional records for Rana bedriagae caralitana in Anatolia. Turkish Journal of Zoology, 26, 381-383.
  • Kumar, P., Kumar, R., Nagpure, N.S., Nautiyal, P., Kushwaha, B. & Dabas, A. (2013). Genotoxicity and antioxidant enzyme activity induced by hexavalent chromium in Cyprinus carpio after in vivo exposure. Drug and Chemical Toxicology, 36, 451-460. DOI:10.3109/01480545.2013.776581.
  • Marquis, O., Miaud, C., Ficetola, G.F., Boscher, A., Mouchet, F., Guittonneau, S. & Devaux, A. (2009). Variation in genotoxic stress tolerance among frog populations exposed to UV and pollutant gradients. Aquatic Toxicology, 95(2), 152-161. DOI: 10.1016/j.aquatox.2009.09.001.
  • Olive, P., Frazer, G. & Banáth, J.P. (1993). Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay, Radiation Research. 136, 130-136.
  • Rajaguru, P., Kalpana, R., Hema, A., Suba, S., Baskarasethupathi, B., Kumar, P.A. & Kalaiselvi, K. (2001). Genotoxicity of some sulfur dyes on tadpoles (Rana hexadactyla) measured using the comet assay. Environmental and Molecular Mutagenesis, 38, 316-22. DOI:10.1002/em.10027.
  • Ralph, S. & Petras, M. (1998). Caged amphibian tadpoles and in situ genotoxicity monitoring of aquatic environments with the alkaline single cell gel electrophoresis (comet) assay. Mutation Research, 413, 235-250. DOI:10.1016/S1383-5718(98)00024-2.
  • Singh, N.P., Mc-Coy, M.T., Tice, R. &Schneider, E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184-191. DOI:10.1016/0014-4827(88)90265.
  • Snegin, E.A. (2011a). Assessment of the state of population gene pools of terrestrial mollusks in conditions of influence of ore dressing combines from the example of Brady baena fruticum Müll (Gastropoda, Pullmonata). Russian Journal of Genetics: Applied Research, 1(5), 379-389. DOI: 10. 1134 / S 20 790 59711050133.
  • Vershinin, V.L. (2008) Morpha striata in the members of the genus Rana (Amphibia, Anura), the reasons of adaptability to environmental changes. Zhurnal Obshchei Biologii, 69(1), 65-71.
  • Wang, M.Z. & Jia, X.Y. (2009). Low levels of lead exposure induce oxidative damage and DNA damage in the testes of the frog Rana nigromaculata. Ecotoxicology, 18,94-99.
  • Zhelev, Z.M., Arnaudov, A.T. & Boyadzhiev, P. (2014). Colour polymorphism, sex ratio and in the populations of Pelophylax ridibundus and Pseudepidalea viridis (Amphibia: Anura) from anthropogenically polluted biotopes in southern Bulgaria and their usage as biondicators. Trakia Journal of Sciences, 12 (1), 1-12.

Endemik Beyşehir kurbağası Pelophylax caralitanus (Arıkan,1988) populasyonlarında Comet Analizi ile DNA hasarın değerlendirilmesi

Yıl 2019, Cilt: 36 Sayı: 2, 109 - 114, 15.06.2019
https://doi.org/10.12714/egejfas.2019.36.2.02

Öz



Bu çalışmada, DNA Comet Assay tekniği kullanılarak, farklı antropojenik kirliliğe sahip Karamık, Eber and Beyşehir Göllerinde yaşayan endemik Beyşehir kurbağası (Pelophylax caralitanus) üç populasyonunda DNA hasar düzeyleri değerlendirilmiştir. Analizler için kurbağa eritrosit hücreleri kullanıldı ve uygun biyotoplardan toplanılan 7 ergin bireyin, her birinden minimum 100 hücre ile analiz edilmiştir. Sonuçlar DNA comet indeksinde kontamine olmuş zonda [(Eber Gölü = Idc=0.22±0.015), (p<0.05)] artış olduğu gösterirken, temiz zonda, hücrelerin çoğunluğunun hasarsız DNA içerdiğini (Idc = 0.08 ± 0.001) göstermiştir. Elde edilen veriler, endemik Beyşehir kurbağasının ekosistemler üzerindeki antropojenik etkinin bayrak türü göstergesi olarak kullanılma olasılığını göstermektedir.




Proje Numarası

KBAG 113Z139 - 16.FENED.07

Kaynakça

  • Arıkan, H. (1988). On a new form Rana ridibunda (Anura: Ranidae) from Turkey. Istanbul Üniversitesi Fen Fakültesi Mecmuası, İstanbul, 53, 81-87.
  • Ashraf, W. (2005). Accumulation of heavy metals in kidney and heart tissues of Epinephelus microdon fish from the Arabian Gulf, Environmental Monitoring and Assessment. 101, 311-316. DOI:10.1007/s10661-005-0298-4.
  • Budak, A., Tok, C.V. & Ayaz, D. (2000). On specimens of Rana ridibunda Pallas, 1771 (Anura: Ranidae) collected from Işıklı Lake (Çivril-Denizli). Turkish Journal of Zoology, 24, 135-137.
  • Cotelle, S. & Férard, J.F. (1999). Comet assay in genetic ecotoxicology, A review. Environmental and Molecular Mutagenesis, 34, 246-255. DOI:10.1002/(SICI)1098-2280.
  • Dautremepuits, C., Paris-Palacios, S., Betoulle, S. & Vernet G. (2004). Modulation in hepatic and head kidney parameters of Carp (Cyprinus carpio) induced by copper and chitosan. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 137, 325-333.
  • Devaux, A., Flammarion, P. & Bernardon, V. (1998). Monitoring of the chemical pollution of the river Rhonethrough measurement of DNA damage and cytochrome P-450IA induction in chub (Leuciscus cephalus). Marine Environmental Research, 46, 257-262. DOI:10.1016/S0141-1136(97)00105.
  • Dhawan, A., Bajpayee, M. & Parmar, D. (2009). Comet assay: a reliable tool for the assessment of DNA damage in different models. Cell Biology and Toxicology, 25, 5-32. DOI:10.1007/s10565-008-9072.
  • El-Zein, R.A., Hastings-Smith, D.A., Ammenheuser, M.M., Trinen-Moslen, M., Gulland, F.M. & Ward, J.B. (2006). Evaluation of two different biomarkers for use in the assessment of toxic chemical exposure in California sea lions (Zalophus californianus). Marine Pollution Bulletin, 52, 104-120. DOI:10.1016/j.marpolbul. 2005.10.007.
  • Erismis U.C. (2018). Age, size, and growth of the Turkish endemic frog Pelophylax caralitanus (Anura: Ranidae). The Anatomical Record, 301, 1224-1234.
  • Erismis, U.C. & Chinsamy, A. (2010). Ontogenetic changes in the epiphyseal cartilage of Rana (Pelophylax) caralitana (Anura: Ranidae). The Anatomical Record, 293(11), 1825-1837. DOI: 10.1002 /ar.21241.
  • Erismis, U.C., Cigerci, I.H. & Konuk, M. (2013). Evaluation of DNA damage in Eurasian Marsh Frogs (Pelophylax ridibundus) by Comet Assay for determination of possible pollution in the different lakes in Central Anatolia, Turkey. The Bulletin of Environmental Contamination and Toxicology, 90, 660-665. DOI:10.1007/s00128-013-0991.
  • Ferreira, C.M., Lombardi, J.V., Machado-Neto, J.G., Bueno-Guimarães, H.M., Soares S.R.C. & Saldiva, P.H.N. (2004). Effects of copper oxychloride in Rana catesheiana tadpoles: toxicological and bioaccumulative aspects. Bulletin of Environmental Contamination and Toxicology, 73, 465-470.
  • Gürkan, M., Hayretdağ, S., Yakın, B.Y. & Tok, C.V. (2012). A preliminary study on micronucleus analysis and nuclear abnormalities in Pelophylax ridibundus (Pallas, 1771) (Amphibia: Anura) specimens collected around Vize (Kırklareli) and Ida Mountains (Çanakkale, Turkey). Ege Journal of Fisheries and Aquatic Sciences, 29, 133-136. DOI: egejfas.2012.29.3.05.
  • Hartmann, M., Schneider, T.R., Pfeil, A., Heinrich, G., Lipscomb, W.N. & Braus, G.H. (2003). Evolution of feedback-inhibited beta/alpha barrel isoenzymes by gene duplication and a single mutation. Proceedings of the National Academy of Sciences of the United States of America, 100(3), 862-7. DOI:10.1073/pnas.0337566100.
  • Huang, D., Zhang, Y., Wang, Y., Xie, Z. & Ji, W. (2007). Assessment of the genotoxicityin toad Bufo raddei exposed to petrochemical contaminants in Lanzhou Region, China. Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 629, 81-8. DOI: 10.1016 /j.mrgentox.2007.01.007.
  • Ismail, M., Khan, Q., Ali, R., Ali, T. & Mobeen, A. (2014). Evaluation of the genotoxicity of chlorpyrifos in common indus valley toad, Bufo stomaticus using alkaline single-cell gel electrophoresis (Comet) assay. The Journal of Agricultural Science 5, 376-382
  • Jdeidi, T., Bilgin, C.C. & Kence, M. (2001). New localities extend the range of Rana bedriagae caralitana Arıkan, 1988 (Anura: Ranidae) further west and suggest specific status. Turkish Journal of Zoology, 25, 153-158.
  • Kalay, M. Ay, O. & Canil, M. (1999). Heavy metal concentration in fish tissues from the northeast Mediterean Sea. The Bulletin of Environmental Contamination and Toxicology, 63, 673-681. DOI: 10.1007/s001289901033.
  • Kaya, U., Çevik, I.E. & Erişmiş, U.C. (2002). New distributional records for Rana bedriagae caralitana in Anatolia. Turkish Journal of Zoology, 26, 381-383.
  • Kumar, P., Kumar, R., Nagpure, N.S., Nautiyal, P., Kushwaha, B. & Dabas, A. (2013). Genotoxicity and antioxidant enzyme activity induced by hexavalent chromium in Cyprinus carpio after in vivo exposure. Drug and Chemical Toxicology, 36, 451-460. DOI:10.3109/01480545.2013.776581.
  • Marquis, O., Miaud, C., Ficetola, G.F., Boscher, A., Mouchet, F., Guittonneau, S. & Devaux, A. (2009). Variation in genotoxic stress tolerance among frog populations exposed to UV and pollutant gradients. Aquatic Toxicology, 95(2), 152-161. DOI: 10.1016/j.aquatox.2009.09.001.
  • Olive, P., Frazer, G. & Banáth, J.P. (1993). Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay, Radiation Research. 136, 130-136.
  • Rajaguru, P., Kalpana, R., Hema, A., Suba, S., Baskarasethupathi, B., Kumar, P.A. & Kalaiselvi, K. (2001). Genotoxicity of some sulfur dyes on tadpoles (Rana hexadactyla) measured using the comet assay. Environmental and Molecular Mutagenesis, 38, 316-22. DOI:10.1002/em.10027.
  • Ralph, S. & Petras, M. (1998). Caged amphibian tadpoles and in situ genotoxicity monitoring of aquatic environments with the alkaline single cell gel electrophoresis (comet) assay. Mutation Research, 413, 235-250. DOI:10.1016/S1383-5718(98)00024-2.
  • Singh, N.P., Mc-Coy, M.T., Tice, R. &Schneider, E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184-191. DOI:10.1016/0014-4827(88)90265.
  • Snegin, E.A. (2011a). Assessment of the state of population gene pools of terrestrial mollusks in conditions of influence of ore dressing combines from the example of Brady baena fruticum Müll (Gastropoda, Pullmonata). Russian Journal of Genetics: Applied Research, 1(5), 379-389. DOI: 10. 1134 / S 20 790 59711050133.
  • Vershinin, V.L. (2008) Morpha striata in the members of the genus Rana (Amphibia, Anura), the reasons of adaptability to environmental changes. Zhurnal Obshchei Biologii, 69(1), 65-71.
  • Wang, M.Z. & Jia, X.Y. (2009). Low levels of lead exposure induce oxidative damage and DNA damage in the testes of the frog Rana nigromaculata. Ecotoxicology, 18,94-99.
  • Zhelev, Z.M., Arnaudov, A.T. & Boyadzhiev, P. (2014). Colour polymorphism, sex ratio and in the populations of Pelophylax ridibundus and Pseudepidalea viridis (Amphibia: Anura) from anthropogenically polluted biotopes in southern Bulgaria and their usage as biondicators. Trakia Journal of Sciences, 12 (1), 1-12.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Uğur Cengiz Erişmiş 0000-0002-1288-3088

Proje Numarası KBAG 113Z139 - 16.FENED.07
Yayımlanma Tarihi 15 Haziran 2019
Gönderilme Tarihi 30 Ekim 2018
Yayımlandığı Sayı Yıl 2019Cilt: 36 Sayı: 2

Kaynak Göster

APA Erişmiş, U. C. (2019). Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988). Ege Journal of Fisheries and Aquatic Sciences, 36(2), 109-114. https://doi.org/10.12714/egejfas.2019.36.2.02