Araştırma Makalesi
17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler
Öz
Bu çalışmada, 17α-Etinilestradiol (EE2; 1, 10 ve 100 ng/l) ve 4-n-Nonilfenolün (NP; 10, 60 ve 200 µg/l) nominal konsantrasyonlarına, semistatik koşullarda kronik olarak (32 gün), maruz bırakılan inci kefalinin karaciğer hepatositnde, sitokrom P4501A (CYP1A) enziminin hepatositlerdeki immunohistokimyasal lokalizasyonu, incelendi. Deneysel uygulamalar sonrasında, karaciğer kesitleri immunohistokimya yöntemi ile boyandı ve değerlendirildi. CYP1A immunreaktivitesi, kontrol grubuna ait bireylerde hepatositlerin sitoplazmasında, gözlenmedi. NP uygulanan gruplarda, CYP1A’nın immunohistokimyasal boyanmasında kontrol bireylerine kıyasla, bir farklılık belirlenmedi. EE2 uygulaması sonucunda, hepatositlerin sitoplazmasında nukleus memranından plazma membranına doğru artan ve orta şiddetli olan immunboyanma gözlendi. Elde edilen sonuçlar bu zenoöstrojenlerin, hepatositlerdeki CYP1A immunboyanması üzerine farklı etkiler gösterdiklerini ortaya koymuştur.
Anahtar Kelimeler
Chalcalburnus tarichi CYP1A karaciğer hepatosit 17α-Etinilestradiol 4-n-Nonilfenol
Destekleyen Kurum
Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı
Proje Numarası
2007-FED-B42
Teşekkür
Bu çalışma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından desteklenmiştir (Proje No: 2007-FED-B42). Desteğinden dolayı Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı’na teşekkür ederiz.
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Toplam 44 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Proje Numarası | 2007-FED-B42 |
| Yayımlanma Tarihi | 18 Eylül 2020 |
| Gönderilme Tarihi | 2 Ağustos 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 3 Sayı: 2 |
