Effect of the Physalis peruviana and Linum usitatissimum Extracts Against Toluene-Induced Oxidative Damages in Kidney and Liver Tissues of Rats
Öz
. Toluen, causes a great environmental problem because it is widely used in the industry. This study was intended to examine the effect of goldenberry, Physalis peruviana (PP) and flaxseed, Linum usitatissimum (LU) extracts on the malondialdehyde (MDA), reduced glutathione (GSH) fatty acid and lipophilic vitamin content in the kidney and liver tissue of Sprague Dawley male rats which were oxidative stressed by toluene. PP and LU were extracted and subjected to vitamin and flavonoid analyses. The rats were divided into four groups as control (C), toluene (T), toluene + P. peruviana (T + PP), toluene + L. usitatissimum (T + LU). T was injected intraperitoneally at a concentration of 0.3 mL/kg. In addition, PP and LU were also injected intraperitoneally at a concentration of 0.5 mL/kg. The treatment was maintained for two months. The rats were decapitated and tissues were removed. Tissue samples were homogenized using buffer solutions. MDA, GSH, lipophilic vitamin, cholesterol and fatty acid content was determined. MDA and GSH concentration were significantly high and low in all toluene-treated groups, respectively (p<0.001). GSH level increased and the MDA concentration decreased in PP and LU treated groups (p<0.01). The palmitic (C16:0), stearic (C18:0), oleic (C18:n-9), docosahexaenoic (C22:6n-3) and arachidonic (C20:4n-6) acid concentrations were mainly higher in the kidney and liver tissues collected from the PP and LU treated groups than those collected from the control group. The K1, delta-tocopherol and alfa–tocopherol acetate vitamin concentrations were higher in PP and LU treated groups than those at group treated with the toxin group. The measured concentrations were similar to those measured in the control group. PP and LU decreased the cholesterol concentration in the kidney and liver tissues (p<0.05 and 0.01, respectively). Our results indicate that antioxidant defense systems of toluene groups have decreased. PP and LU possessed a protective feature against the oxidative stress caused by toluen.
Anahtar Kelimeler
Physalis peruviane Linum usitatissimum Toluene Malondialdehyde Antioxidant Rat
Destekleyen Kurum
Firat University Research Fund
Proje Numarası
FÜBAP Project number FF.12.11
Kaynakça
- Abdel Moneim A.E., Prevention of carbon tetrachloride (CCl4) induced toxicity in testes of rats treated with Physalis peruviana L. fruit,. Toxicology and Industrial Health , 32-6 (2016) 1064–1073.
- Abdel-Moneim A.E., Bauomy A.A., Diab M.M., Shata M.T., Al-Olayan E.M. and El-Khadragy M.F.,The Protective Effect of Physalis peruviana L. Against Cadmium-Induced Neurotoxicity in Rats, Biological Trace Element Research., 160-3 (2014) 392-399.
- Abdel-Moneim A.E., Dkhil M.A. and Al-Quraishy S., The protective effect of flaxseed oil on lead acetate induced renal toxicity in rats, Journal of Hazardous Materials., 194-1 (2011a) 250–255.
- Abdel-Moneim A.E., Dkhil M.A. and Al-Quraishy S., The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity, Biological Trace Element Research 143-1 (2011b) 457-467.
- Abdel-Salam, O.M., Sleem, A. A., Khadrawy, Y.A., and Morsy, F.A. Prevention of toluene- induced brain neurodegeneration by atropine and neostigmine. Journal of Basic Pharmacology and Toxicology, 4(1), (2020).1-9.
- Arun M. and Asha V.V., Preliminary studies on antihepatotoxic effect of Physalis peruviana Linn. against carbon tetrachloride induced acute liver injury in rats, The Journal of Ethnopharmacology 111-1 (2007) 110–114.
- Arvindkumar E.G., Suresh S. and Jadhav S.L., Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia reperfusion injury in rats, Arab Journal of Urology, 9-3 (2011) 215-221.
- Aylward L.L., Barton H.A. and Hays S.M., Biomonitoring equivalents (BE) dossier for toluene (CAS No. 108 88-3), Regulatory Toxicology and Pharmacology, 51-3 (2008) 27–36.
- Bayil S., Cicek H., Cimenci I.G. and Hazar M., How volatile organic compounds affect free radical and antioxidant enzyme activity in textile workers, Journal of industrial hygiene and toxicology., 59-4 (2008) 283–287.
- Christie W.W., Gas chromatography and lipids, The Oil Pres, Glaskow (1990).
- Costa C., Pasquale R., Silvari V., Barbaro M. and Catania S., In vitro evaluation of oxidative damage from organic solvent vapors on human skin, Toxicology in Vitro ., 20-3 (2005) 324–331.
- EPA., USA., Toxicological Review of Toluene, EPA/635/R-05/004 (1959).
- Fang S.T., Liu J.K. and Li B., Ten new withanolides from Physalis peruviana, Steroids., 77-1 (2012) 36–44.
- Gerin M., Siemiatychi J., Desy M. and Krewski D., Associations between several sites of cancer and occupational exposure to benzene, toluene, xylene, and styrene: results of a case-control study in Montreal, American Journal of Industrial Medicine., 34-2 (1998) 144–156.
- Gotohda T., Nishimura A. and Morita K., Immuno histochemical studies on early stage of hepatic damage induced by subacute inhalation of toluene vapor in rats, Journal of Applied Toxicology, 29-6 (2009) 505-509.
- Hara A. and Radin N.S., Lipid extraction of tissues with a low-toxicity solvent, Journal of Biochemistry and Analytical Biochemistry ., 90-1 (1978) 420-426.
- Hendawi M.Y., Alam R.T. and Abdellatief S.A., Ameliorative effect of flaxseed oil against thiacloprid- induced toxicity in rats: hematological, biochemical, and histopathological study, Environmental science and pollution research international 23-12 (2016) 11855-11863.
- Huff J., Absence of carcinogenic activity in Fischer rats and B6C3F1 mice following 103-week inhalation exposures to toluene, International Journal of Occupational Safety and Health ., 9-2 (2003) 138- 146.
- Jamshidi-Kia F., Lorigooini Z. and Amini-Khoei H. . Medicinal plants: past history and future perspective, Journal of Herbmed Pharmacology 7(1) (2018) 1-7.
- Katsanidis E. and Addis P.B., Novel HPLC analysis of tocopherols and cholesterol in tissue, Free Radical Biology and Medicine ree Radic. Biol. Med., 27 – 1 (11-12) (1999) 1137-1140.
- Kayhan F.E.B., Koç N.D., Contuk G., Muşlu M.N. and Sesal N.C., Sıçan Böbrek Dokusunda Endosulfan ve Malathion’un Oluşturduğu Yapısal Değişiklikler, Journal of Science and Arts J., 12-1 (2009) 43-51.
- Kris-Etherton P.M., Lefevre M., Beecher G.R., Gross M.D., Keen C.L. and Etherton T.D., Bioactivecompounds in nutrition and health-research methodologies for establishing biological funtion: the antioxidant and anti-inflammatory effects of flavonoids on atherosclerosis, The Annual Review of Nutrition 24-1 (2004) 511-538.
- Lowry O.H., Rosenbrough N.J., Farr A.L. and Randall R.J., Protein measurement with the folin-phenol reagent, Turkish Journal of Biochemistry 193-1 (1951) 265-277.
- Meek M. and Chan P., Toluene: evaluation of risks to human health from environmental exposure in Canada, Environmental Carcinogenesis and Ecotoxicology Reviews, 12-1 (1994) 507–515.
- Moro A.M., Charao M., Brucker N., Bulcao R., Freitas F., Guerreiro G., Baierle M., Nascimento S., Waechter F., Hirakata V., Linden R., Thiesen F.V. and Garcia S.C., Evaluation of genotoxicity and oxidative damage in painters exposed to low levels of tolüene, Mutation Research, 746-1 (2012) 42– 48.
- Moro A.M., Charão M., Brucker N., Bulcão R., Freitas F., Guerreiro G., Baierle M., Nascimento S., Waechter F., Hirakata V., Linden R., Thiesen F.V. and Garcia S.C., Effects of low-level exposure to xenobiotics present in paints on oxidative stress in workers, Science of the Total Environment, 408-20 (2010) 4461–4467.
- Nasri H., World kidney day: acute kidney injury; a public health aware, Iranian Journal of Public Health 42-1 (2013) 338-40.
- Ohkawa H., Ohishi N. and Yagi K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Analytical Biochemistry., 95-2 (1979) 351-358.
- Ramadan M.F. and Moersel J., Oil goldenberry (Physalis peruviana L.), The Journal of Agricultural and Food Chemistry, 51-4 (2003) 969−974.
- Scalzo J., Politi A., Pellegrini N., Mezzetti B. and Battino M., Plant genotype affects total antioxidant capacity and phenolic contents in fruit, Nutrition, 2-1 (2005) 207–213.
- Tapiero H., Tew K.D., Ba G.N. and Mathe G., Do they play a role in the prevention of human pathologies, Biomedicine and Pharmacotherapy, 56-4 (2002) 200–207.
- Teare, J.P., Punchard, N.A., Powell, J.J., Lumb, P.J., Mitchel, W.D. and Thompson, R.P.H. Automated spectrophotometric method for determining oxidized and reduced glutathione in liver. Clinical Chemistry, 39-4 (1993) 686-689.
- Trovato M. and Ballabio C., Botanical products: general aspects. In: Food supplements containing botanicals: benefits, side effects and regulatory aspects, Cham: Springer., (2018) 3–26.
- Wu S.J., Ng L.T., Huang Y.M., Lin D.L., Wang S.S., Huang S.N. and Lin C.C. Antioxidant activities of Physalis peruviana, Biological and Pharmaceutical Bulletin., 28-6 (2005) 963–966.
- Zu Y., Li C., Fu Y. and Zhao C., Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD, Journal of pharmaceutical and biomedical analysis., 41-3 (2006) 714–719.
Öz
Proje Numarası
FÜBAP Project number FF.12.11
Kaynakça
- Abdel Moneim A.E., Prevention of carbon tetrachloride (CCl4) induced toxicity in testes of rats treated with Physalis peruviana L. fruit,. Toxicology and Industrial Health , 32-6 (2016) 1064–1073.
- Abdel-Moneim A.E., Bauomy A.A., Diab M.M., Shata M.T., Al-Olayan E.M. and El-Khadragy M.F.,The Protective Effect of Physalis peruviana L. Against Cadmium-Induced Neurotoxicity in Rats, Biological Trace Element Research., 160-3 (2014) 392-399.
- Abdel-Moneim A.E., Dkhil M.A. and Al-Quraishy S., The protective effect of flaxseed oil on lead acetate induced renal toxicity in rats, Journal of Hazardous Materials., 194-1 (2011a) 250–255.
- Abdel-Moneim A.E., Dkhil M.A. and Al-Quraishy S., The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity, Biological Trace Element Research 143-1 (2011b) 457-467.
- Abdel-Salam, O.M., Sleem, A. A., Khadrawy, Y.A., and Morsy, F.A. Prevention of toluene- induced brain neurodegeneration by atropine and neostigmine. Journal of Basic Pharmacology and Toxicology, 4(1), (2020).1-9.
- Arun M. and Asha V.V., Preliminary studies on antihepatotoxic effect of Physalis peruviana Linn. against carbon tetrachloride induced acute liver injury in rats, The Journal of Ethnopharmacology 111-1 (2007) 110–114.
- Arvindkumar E.G., Suresh S. and Jadhav S.L., Renoprotective effect of Linum usitatissimum seeds through haemodynamic changes and conservation of antioxidant enzymes in renal ischaemia reperfusion injury in rats, Arab Journal of Urology, 9-3 (2011) 215-221.
- Aylward L.L., Barton H.A. and Hays S.M., Biomonitoring equivalents (BE) dossier for toluene (CAS No. 108 88-3), Regulatory Toxicology and Pharmacology, 51-3 (2008) 27–36.
- Bayil S., Cicek H., Cimenci I.G. and Hazar M., How volatile organic compounds affect free radical and antioxidant enzyme activity in textile workers, Journal of industrial hygiene and toxicology., 59-4 (2008) 283–287.
- Christie W.W., Gas chromatography and lipids, The Oil Pres, Glaskow (1990).
- Costa C., Pasquale R., Silvari V., Barbaro M. and Catania S., In vitro evaluation of oxidative damage from organic solvent vapors on human skin, Toxicology in Vitro ., 20-3 (2005) 324–331.
- EPA., USA., Toxicological Review of Toluene, EPA/635/R-05/004 (1959).
- Fang S.T., Liu J.K. and Li B., Ten new withanolides from Physalis peruviana, Steroids., 77-1 (2012) 36–44.
- Gerin M., Siemiatychi J., Desy M. and Krewski D., Associations between several sites of cancer and occupational exposure to benzene, toluene, xylene, and styrene: results of a case-control study in Montreal, American Journal of Industrial Medicine., 34-2 (1998) 144–156.
- Gotohda T., Nishimura A. and Morita K., Immuno histochemical studies on early stage of hepatic damage induced by subacute inhalation of toluene vapor in rats, Journal of Applied Toxicology, 29-6 (2009) 505-509.
- Hara A. and Radin N.S., Lipid extraction of tissues with a low-toxicity solvent, Journal of Biochemistry and Analytical Biochemistry ., 90-1 (1978) 420-426.
- Hendawi M.Y., Alam R.T. and Abdellatief S.A., Ameliorative effect of flaxseed oil against thiacloprid- induced toxicity in rats: hematological, biochemical, and histopathological study, Environmental science and pollution research international 23-12 (2016) 11855-11863.
- Huff J., Absence of carcinogenic activity in Fischer rats and B6C3F1 mice following 103-week inhalation exposures to toluene, International Journal of Occupational Safety and Health ., 9-2 (2003) 138- 146.
- Jamshidi-Kia F., Lorigooini Z. and Amini-Khoei H. . Medicinal plants: past history and future perspective, Journal of Herbmed Pharmacology 7(1) (2018) 1-7.
- Katsanidis E. and Addis P.B., Novel HPLC analysis of tocopherols and cholesterol in tissue, Free Radical Biology and Medicine ree Radic. Biol. Med., 27 – 1 (11-12) (1999) 1137-1140.
- Kayhan F.E.B., Koç N.D., Contuk G., Muşlu M.N. and Sesal N.C., Sıçan Böbrek Dokusunda Endosulfan ve Malathion’un Oluşturduğu Yapısal Değişiklikler, Journal of Science and Arts J., 12-1 (2009) 43-51.
- Kris-Etherton P.M., Lefevre M., Beecher G.R., Gross M.D., Keen C.L. and Etherton T.D., Bioactivecompounds in nutrition and health-research methodologies for establishing biological funtion: the antioxidant and anti-inflammatory effects of flavonoids on atherosclerosis, The Annual Review of Nutrition 24-1 (2004) 511-538.
- Lowry O.H., Rosenbrough N.J., Farr A.L. and Randall R.J., Protein measurement with the folin-phenol reagent, Turkish Journal of Biochemistry 193-1 (1951) 265-277.
- Meek M. and Chan P., Toluene: evaluation of risks to human health from environmental exposure in Canada, Environmental Carcinogenesis and Ecotoxicology Reviews, 12-1 (1994) 507–515.
- Moro A.M., Charao M., Brucker N., Bulcao R., Freitas F., Guerreiro G., Baierle M., Nascimento S., Waechter F., Hirakata V., Linden R., Thiesen F.V. and Garcia S.C., Evaluation of genotoxicity and oxidative damage in painters exposed to low levels of tolüene, Mutation Research, 746-1 (2012) 42– 48.
- Moro A.M., Charão M., Brucker N., Bulcão R., Freitas F., Guerreiro G., Baierle M., Nascimento S., Waechter F., Hirakata V., Linden R., Thiesen F.V. and Garcia S.C., Effects of low-level exposure to xenobiotics present in paints on oxidative stress in workers, Science of the Total Environment, 408-20 (2010) 4461–4467.
- Nasri H., World kidney day: acute kidney injury; a public health aware, Iranian Journal of Public Health 42-1 (2013) 338-40.
- Ohkawa H., Ohishi N. and Yagi K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Analytical Biochemistry., 95-2 (1979) 351-358.
- Ramadan M.F. and Moersel J., Oil goldenberry (Physalis peruviana L.), The Journal of Agricultural and Food Chemistry, 51-4 (2003) 969−974.
- Scalzo J., Politi A., Pellegrini N., Mezzetti B. and Battino M., Plant genotype affects total antioxidant capacity and phenolic contents in fruit, Nutrition, 2-1 (2005) 207–213.
- Tapiero H., Tew K.D., Ba G.N. and Mathe G., Do they play a role in the prevention of human pathologies, Biomedicine and Pharmacotherapy, 56-4 (2002) 200–207.
- Teare, J.P., Punchard, N.A., Powell, J.J., Lumb, P.J., Mitchel, W.D. and Thompson, R.P.H. Automated spectrophotometric method for determining oxidized and reduced glutathione in liver. Clinical Chemistry, 39-4 (1993) 686-689.
- Trovato M. and Ballabio C., Botanical products: general aspects. In: Food supplements containing botanicals: benefits, side effects and regulatory aspects, Cham: Springer., (2018) 3–26.
- Wu S.J., Ng L.T., Huang Y.M., Lin D.L., Wang S.S., Huang S.N. and Lin C.C. Antioxidant activities of Physalis peruviana, Biological and Pharmaceutical Bulletin., 28-6 (2005) 963–966.
- Zu Y., Li C., Fu Y. and Zhao C., Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD, Journal of pharmaceutical and biomedical analysis., 41-3 (2006) 714–719.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | Research Articles |
| Yazarlar | |
| Proje Numarası | FÜBAP Project number FF.12.11 |
| Yayımlanma Tarihi | 2 Mayıs 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 4 Sayı: 1 |
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