In Vitro Compatibility of Entomopathogenic Fungi Beauveria Bassiana (Bals.) Vuill. with Different Fungicides
Öz
Beauveria bassiana (Balsamo) Vuillemin is one of the entomopathogenic fungi used against broad host insects and plant pathogens. Fungicides have side effects on entomopathogenic fungi. In vitro assay was performed to examine the compatibility of B. bassiana with eight commonly used fungicides (Azoxystrobin 75 g/l+Metalaxyl-m 37.5 g/l+ Fludioxonil 12.5 g/l FS, Boscalid 25%+ Pyraclostrobin 12% WG, Copper Hydroxide 361.1 g/l SC, Azoxystrobin 250 g/l SC, Triticonazole 80 g/l + Pyraclostrobin 40 g/l FS, Fludioxonil 12.5 g/l + Metalaxyl 10 g/l SC, Captan 50% WP, Tebuconazole 250 g/l EC) using contact application technique. Fungicides at various concentrations (Recommend Dose-RD, half of the Recommend Dose-0.5 x RD and twice the Recommend Dose-2 x RD) were mixed in Potato Dextrose Agar (PDA) media post-autoclaving. Approximately 25 ml of the mixture was poured into a Petri dish (90 mm) and allowed to cool. Mycelium disc (5 mm in diameter) was taken from 14-days-old B. bassiana grown on PDA using a sterile cork borer and placed in the center of each Petri dish containing fungicide + PDA. PDA plates without fungicide were used as a control. Petri dishes were incubated in the dark at 25±1 °C for 14 days. In vitro experiments were carried out with three replicates depending on a completely randomized plots design. In the study, the compatible fungicide with B. bassiana ET 10 isolate was found to be Copper Hydroxide at 0.5 x RD concentration. Azoxystrobin and Copper Hydroxide were compatible with Bb 18 isolate. Only two (Azoxystrobin + Metalaxyl-m + Fludioxonil and Tebuconazole) of the eight fungicides completely inhibited the mycelial growth of ET 10 isolate and were found harmful. Azoxystrobin + Metalaxyl-m + Fludioxonil completely inhibited the growth of Bb 18 isolate and was not found compatible. This study clearly shows that fungicides have the potential to inhibit the mycelial growth of entomopathogenic fungi under in vitro conditions. However, these results need to be further verified in vitro under both greenhouse and open-field conditions.
Anahtar Kelimeler
Entomopathogenic fungi Beauveria bassiana Fungicides Compatibility Inhibition Harmful
Teşekkür
The authors thank to Assoc. Prof. Dr. Elif Tozlu (Atatürk University, Erzurum, Türkiye), Prof. Dr. Salih Karabörklü (Sakarya Applied Sciences University, Sakarya, Türkiye) for kindly providing local isolates of B. bassiana and ÖZALTIN Agriculture Company for kindly providing ASTRADYN fungicide.
Kaynakça
- Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B. 2002. The strobilurin fungicides. Pest Manag Sci, 58: 649-662.
- Beilhartz VC, Parberry DG, Swart HJ. 1982. Dodine: A selective agent for certain soil fungi. Transact the Brit Mycol Soci, 79: 507-511.
- Celar F, Kos K. 2020. In vitro compatibility of Beauveria bassiana strain ATCC 74040 with copper fungicides. J Appl Entomol, 144: 388-395.
- De Olivera RC, Neves PMOJ. 2004. Biological control compatibility of Beauveria bassiana with acaricides. Neotrop Entomol, 33(3): 353-358.
- Er MK, Gökçe A. 2004. Effects of selected pesticides used against glasshouse tomato pests on colony growth and conidial germination of Paecilomyces fumosoroseus. Biol Control, 31: 398-404.
- Erdoğan O, Sağlan Z. 2023. Antifungal activity of local isolates of Beauveria bassiana (Balsamo) Vuillemin against Verticillium dahliae Kleb. causing wilt disease of cotton. Egyptian J Biol Pest Cont, 33: 52.
- Faion M. 2004. Toxicidade de agrotoxicos utilizados no controle de Bemisia tabaci biotipo b sobre fungus entomopatogeˆnicos. MSc Thesis, Universidad de Sa˜o Paulo, Sa˜o Paulo, Brazil, pp: 86.
- Fiedler Z, Sosnowska D. 2017. Side effects of fungicides and insecticides on entomopathogenic fungi in vitro. J Plant Protec Res, 57: 355-360.
- Gatarayiha MC, Laing MD, Miller RM. 2010. In vitro effects of flutriafol and azoxystrobin on Beauveria bassiana and its efficacy against Tetranychus urticae. Pest Manag Sci, 66(7): 773-778.
- Goettel MS, Eilenberg J, Glare TR. 2010. Entomopathogenic fungi and their role in regulation of insect populations. In insect control: Biological and synthetic agents, ed. by Gilbert LI and Gill DS. Academic Press, San Diego, US, pp: 387-431.
- Hassan SA. 1989. Testing methodology and the concept of the IOBC / WPRS Working Group. In: Jepson, P. C. (Ed.). Pesticides and non-target invertebrates. Intercept, Wimborne, Dorset, UK, pp: 1-8.
- Khan S, Bagwan NB, Fatima S, Iqbal MA. 2012. In vitro compatibility of two entomopathogenic fungi with selected insecticides, fungicides, and plant growth regulators. Libyan Agric Res Center J Inter, 3(1): 36-41.
- Kouassi M, Coderre D, Todorova I. 2003. Effects of the timing of applications on the incompatibility of three fungicides and one isolate of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina). J Appl Entomol, 127: 421-426.
- Li DP, Holdom DG. 1994. Effects of pesticides on growth and sporulation of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes). J Invertebr Pathol, 63: 209-211.
- Loureiro ESde A, Moino Jr, Arnosti A, de souza GC. 2002. Efeito de produtos fitossanitarios quimicos utilizados em alface e crisantemo sobre fungos entomopatogenicos. Neotrop Entomol, 31: 263-269.
- Luz C, Bastos Netto MC, Rocha LFN. 2007. In vitro susceptibility to fungicides by invertebrate pathogenic and saprobic fungi. Mycopathol, 164: 39-47.
- Martins F, Soares ME, Oliveira I, Pereira JA, Bastos ML, Baptista P. 2012. Tolerance and bioaccumulation of copper by the entomopathogen Beauveria bassiana (Bals.-Criv.) Vuill. exposed to various copper-based fungicides. Bull Environ Contam Toxicol, 89(1): 53-60.
- Olmert I, Kenneth RG. 1974. Sensitivity of entomopathogenic fungi, Beauveria bassiana, Verticillium lecanii and Verticillium sp. to fungicides and insecticides. Environ Entomol, 3: 33-39.
- Ownley B, Gwinn KD, Vega FE. 2010. Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. BioControl, 55: 113-128.
- Rachappa V, Lingappa S, Patil RK. 2007. Effect of agrochemicals on growth and sporulation of Metarhizium anisopliae (Metschnikoff) sorokin. Karnataka J Agric Sci, 20: 410-413.
- Rath AC. 2000. The use of entomopathogenic fungi for control of termites. Biocontrol Sci and Technol, 10: 563-581.
- Reddy S, Reddy MLN, Pushpalatha M. 2018. Interaction of fungicides with bio-control agents. J Entomol and Zool Stud, 6(4): 545-551.
- Rehner SA, Minnis AM, Sung GH, Luangsa-ard JJ, Devotto L, Humber RA. 2011. Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycol, 103: 1055-1073.
- Roberts DW. 1989. Word picture of biological control of insects by fungi. Memorias do Inst Oswaldo Cruz, 84: 89-100.
- Sevim A, Sevim E, Demirbağ Z. 2015. Entomopatojenik fungusların genel biyolojileri ve Türkiye’de zararlı böceklerin mücadelesinde kullanılma potansiyelleri. Erzincan Üniv Fen Bilimleri Enst Derg, 8(1): 115-147.
- Shah FA, Ansari MA, Watkins J, Phelps Z, Cross J, Butt TM. 2009. Influence of commercial fungicides on the germination, growth, and virulence of four species of entomopathogenic fungi. Biocontrol Sci Technol, 19: 743-753.
- Sosa-Gomez DR, Delpin KE, Moscardi F, Nozaki MH. 2003. The impact of fungicides on Nomuraea rileyi (Farlow) samson epizootics and on populations of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae), on soybean. Neotrop Entomol, 32: 287-291.
- Tozlu E, Kotan R, Tozlu G. 2017. The investigation of Beauveria bassiana (Ascomycota: Hypocreales) as a biocontrol agent of rose-stem sawfly, Syrista parreyssii (Spinola, 1843) (Hymenoptera: Symphyta; Cephidae) larvae. Fresen Environ Bull, 26(12): 7091-7100.
- Wang H, Yan Y, Wang J, Zhang H, Qi W. 2012. Production and characterization of antifungal compounds produced by Lactobacillus plantarum IMAU10014. PloS ONE, 7(1): e29452.
- Zimmermann G. 2007. Review of safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Sci Technol, 17(6): 553-596.
Öz
Kaynakça
- Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B. 2002. The strobilurin fungicides. Pest Manag Sci, 58: 649-662.
- Beilhartz VC, Parberry DG, Swart HJ. 1982. Dodine: A selective agent for certain soil fungi. Transact the Brit Mycol Soci, 79: 507-511.
- Celar F, Kos K. 2020. In vitro compatibility of Beauveria bassiana strain ATCC 74040 with copper fungicides. J Appl Entomol, 144: 388-395.
- De Olivera RC, Neves PMOJ. 2004. Biological control compatibility of Beauveria bassiana with acaricides. Neotrop Entomol, 33(3): 353-358.
- Er MK, Gökçe A. 2004. Effects of selected pesticides used against glasshouse tomato pests on colony growth and conidial germination of Paecilomyces fumosoroseus. Biol Control, 31: 398-404.
- Erdoğan O, Sağlan Z. 2023. Antifungal activity of local isolates of Beauveria bassiana (Balsamo) Vuillemin against Verticillium dahliae Kleb. causing wilt disease of cotton. Egyptian J Biol Pest Cont, 33: 52.
- Faion M. 2004. Toxicidade de agrotoxicos utilizados no controle de Bemisia tabaci biotipo b sobre fungus entomopatogeˆnicos. MSc Thesis, Universidad de Sa˜o Paulo, Sa˜o Paulo, Brazil, pp: 86.
- Fiedler Z, Sosnowska D. 2017. Side effects of fungicides and insecticides on entomopathogenic fungi in vitro. J Plant Protec Res, 57: 355-360.
- Gatarayiha MC, Laing MD, Miller RM. 2010. In vitro effects of flutriafol and azoxystrobin on Beauveria bassiana and its efficacy against Tetranychus urticae. Pest Manag Sci, 66(7): 773-778.
- Goettel MS, Eilenberg J, Glare TR. 2010. Entomopathogenic fungi and their role in regulation of insect populations. In insect control: Biological and synthetic agents, ed. by Gilbert LI and Gill DS. Academic Press, San Diego, US, pp: 387-431.
- Hassan SA. 1989. Testing methodology and the concept of the IOBC / WPRS Working Group. In: Jepson, P. C. (Ed.). Pesticides and non-target invertebrates. Intercept, Wimborne, Dorset, UK, pp: 1-8.
- Khan S, Bagwan NB, Fatima S, Iqbal MA. 2012. In vitro compatibility of two entomopathogenic fungi with selected insecticides, fungicides, and plant growth regulators. Libyan Agric Res Center J Inter, 3(1): 36-41.
- Kouassi M, Coderre D, Todorova I. 2003. Effects of the timing of applications on the incompatibility of three fungicides and one isolate of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina). J Appl Entomol, 127: 421-426.
- Li DP, Holdom DG. 1994. Effects of pesticides on growth and sporulation of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes). J Invertebr Pathol, 63: 209-211.
- Loureiro ESde A, Moino Jr, Arnosti A, de souza GC. 2002. Efeito de produtos fitossanitarios quimicos utilizados em alface e crisantemo sobre fungos entomopatogenicos. Neotrop Entomol, 31: 263-269.
- Luz C, Bastos Netto MC, Rocha LFN. 2007. In vitro susceptibility to fungicides by invertebrate pathogenic and saprobic fungi. Mycopathol, 164: 39-47.
- Martins F, Soares ME, Oliveira I, Pereira JA, Bastos ML, Baptista P. 2012. Tolerance and bioaccumulation of copper by the entomopathogen Beauveria bassiana (Bals.-Criv.) Vuill. exposed to various copper-based fungicides. Bull Environ Contam Toxicol, 89(1): 53-60.
- Olmert I, Kenneth RG. 1974. Sensitivity of entomopathogenic fungi, Beauveria bassiana, Verticillium lecanii and Verticillium sp. to fungicides and insecticides. Environ Entomol, 3: 33-39.
- Ownley B, Gwinn KD, Vega FE. 2010. Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. BioControl, 55: 113-128.
- Rachappa V, Lingappa S, Patil RK. 2007. Effect of agrochemicals on growth and sporulation of Metarhizium anisopliae (Metschnikoff) sorokin. Karnataka J Agric Sci, 20: 410-413.
- Rath AC. 2000. The use of entomopathogenic fungi for control of termites. Biocontrol Sci and Technol, 10: 563-581.
- Reddy S, Reddy MLN, Pushpalatha M. 2018. Interaction of fungicides with bio-control agents. J Entomol and Zool Stud, 6(4): 545-551.
- Rehner SA, Minnis AM, Sung GH, Luangsa-ard JJ, Devotto L, Humber RA. 2011. Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycol, 103: 1055-1073.
- Roberts DW. 1989. Word picture of biological control of insects by fungi. Memorias do Inst Oswaldo Cruz, 84: 89-100.
- Sevim A, Sevim E, Demirbağ Z. 2015. Entomopatojenik fungusların genel biyolojileri ve Türkiye’de zararlı böceklerin mücadelesinde kullanılma potansiyelleri. Erzincan Üniv Fen Bilimleri Enst Derg, 8(1): 115-147.
- Shah FA, Ansari MA, Watkins J, Phelps Z, Cross J, Butt TM. 2009. Influence of commercial fungicides on the germination, growth, and virulence of four species of entomopathogenic fungi. Biocontrol Sci Technol, 19: 743-753.
- Sosa-Gomez DR, Delpin KE, Moscardi F, Nozaki MH. 2003. The impact of fungicides on Nomuraea rileyi (Farlow) samson epizootics and on populations of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae), on soybean. Neotrop Entomol, 32: 287-291.
- Tozlu E, Kotan R, Tozlu G. 2017. The investigation of Beauveria bassiana (Ascomycota: Hypocreales) as a biocontrol agent of rose-stem sawfly, Syrista parreyssii (Spinola, 1843) (Hymenoptera: Symphyta; Cephidae) larvae. Fresen Environ Bull, 26(12): 7091-7100.
- Wang H, Yan Y, Wang J, Zhang H, Qi W. 2012. Production and characterization of antifungal compounds produced by Lactobacillus plantarum IMAU10014. PloS ONE, 7(1): e29452.
- Zimmermann G. 2007. Review of safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Sci Technol, 17(6): 553-596.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Ziraat Mühendisliği |
| Bölüm | Research Articles |
| Yazarlar | |
| Erken Görünüm Tarihi | 30 Haziran 2023 |
| Yayımlanma Tarihi | 1 Temmuz 2023 |
| Gönderilme Tarihi | 24 Mayıs 2023 |
| Kabul Tarihi | 22 Haziran 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 6 Sayı: 4 |
