Araştırma Makalesi
Yıl 2022,
Cilt: 5 Sayı: 4, 464 - 469, 01.10.2022
Öz
In this study, pin and thrum flowered wild primrose (Primula vulgaris= Syn: Primula acaulis) populations, which are naturally distributed in Black Sea Region in Türkiye, were cultivated and their pollen viability, pollen tube growth and embryo development stages were investigated. As a result of the study, pollen viability in pollen of the pin flower type was 83.10%, while the in vitro germination rate was 69.43%. The viability and in vitro germination rates of pollen of thrum flower type were 84.91% and 67.92%, respectively. As a result of the squash preparation examinations, it was observed that the pollen tubes of both types started to germinate on the stigma on the 1 DAP (Day after pollination). On 4 DAP, it was determined that pollen tubes penetrated to the ovule. Embryo developmental stages examinations showed that, zygote formation occurred on 6 DAP in the pin flower type; while on the 20 DAP, the embryo was in the heart stage and finally on the 40 DAP, it was seen that it formed a mature cotyledonary stage embryo. Endosperm in the thrum type started to develop faster than the pin type and formed on the 6 and 7 DAP. In the thrum flower type, the proembryo formed on 10 DAP, but embryo formation could only be seen on 30 DAP. In the ovary examinations performed on the 40 DAP, some embryos were in the cotyledon stage while some were still in the globular or heart stage. It was also determined that no embryos were found in some ovules.
Anahtar Kelimeler
Fertilization Germination Histology Primrose Pollen viability
Destekleyen Kurum
Ondokuz Mayıs Üniversitesi
Proje Numarası
PYO.ZRT.1901.18.004
Teşekkür
This study was supported by Ondokuz Mayıs University with the project number: PYO.ZRT.1901.18.002
Kaynakça
- Akhalkatsi M, Gvaladze G, Taralashvili N. 1998. Embryology of Primula algida and Primula amoena (Primulaceae). Bulletin Georgian Nat Acad Sci, 157(1): 98-100.
- Aronne G, Iovane M, Strumia S. 2021. Temperature and humidity affect pollen viability and may trigger distyly disruption in threatened species. Annali Di Botanica, 11: 77-82.
- Bateson W, Gregory RP. 1905. On the inheritance of heterostylism in Primula. Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 76(513): 581-586.
- Calahan CN, Gliddon C. 1985. Genetic neighbourhood sizes in Primula vulgaris. Heredity, 54: 65-70.
- Cocker JM, Wright J, Li J, Swarbreck D, Dyer S, Caccamo M, Gilmartin PM. 2018. Primula vulgaris (primrose) genome assembly, annotation and gene expression, with comparative genomics on the heterostyly supergene. Sci Reports, 8(1): 1-13.
- Gachechiladze MI. 1993. The embryology of Primula bayernii (Primulaceae). Bot J, 78: 93-96.
- Genç S, Soysal Mİ. 2018. Parametric and nonparametric post hoc tests. BSJ Eng Sci, 1(1): 18-27.
- Grouh MSH, Sousaraei N, Akbari M, Rahimi V, Bayat H. 2015. Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation. Turkish J Agri Forest, 39(4): 596-600.
- Gündoğan Z, Coşkunçelebi K, Gültepe M. 2019. Contribution to infra-specific taxonomy of Primula vulgaris Huds. (Primulaceae) along an altitudinal gradient in Turkey. Taiwania, 64(3): 249-257.
- Jia Y, Zhang QX, Pan HT, Wang SQ, Liu QL, Sun LX. 2014. Callus induction and haploid plant regeneration from baby primrose (Primula forbesiiFranch.) anther culture. Scientia Hort, 176: 273-281.
- Johansen DA. 1940. Plant microtechnique. McGraw-Hill Book Company Inc, London, UK, pp: 530.
- Johri BM, Ambegaokar KB, Srivastava PS. 1992. Primulales. In comparative embryology of angiosperms. Springer, Berlin, Heidelberg, Germany, pp: 629-635.
- Karabıyık Ş, Eti S. 2020. Nucellar embryony and formation mechanism in Sour Orange. Yuzuncu Yil Univ J Agri Sci, 30(4): 761-771.
- Karabıyık Ş. 2022. Effects of temperature on pollen viability and in vivo pollen tube growth in Citrus sinensis. J App Botany Food Qual, 95: 100-104.
- Keller B, Vos JM, Schmidt‐Lebuhn AN, Thomson JD, Conti E. 2016. Both morph and species dependent asymmetries affect reproductive barriers between heterostylous species. Ecol Evol, 6(17): 6223-6244.
- Li J, Webster MA, Smith MC, Gilmartin PM. 2011. Floral heteromorphy in Primula vulgaris: Progress toward sisolation and characterization of the S locus. Ann Bot, 108: 715-726.
- Li J, Webster MA, Wright J, Cocker J, Smith MC, Badakshi F, Heslop-Harrison P, Gilmartin PM. 2015. Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization. New Phytol, 208: 137-148.
- Prajapati DN, Knox JF, Emmons J, Saeian K, Csuka ME, Binion DG. 2003. Leflunomide treatment of crohn's disease patients intolerant to standard immunomodulator therapy. J Clin Gastroenterol, 37: 125-133.
- Smith WW, Forrest G. 1929. The sections of the genus Primula. J Hortic Soc, 54: 1-50.
- Tütüncü M, Mendi YY. 2020. Evaluation of pollen tube growth and fertilization via histological analysis in Cyclamen persicum. Acta Hortic, 1283: 21-26.
- Uce İ, Tunçtürk M. 2014. Hakkâri’ de doğal olarak yetişen ve yaygın olarak kullanılan bazı yabani bitkiler. Biyol Bil Araş Derg, 7(2): 21-25.
- Webster MA, Gilmartin PM. 2003. A comparison of early floral ontogeny in wild-type and floral homeotic mutant phenotypes of Primula. Planta, 216(6): 903-917.
- Webster MA, Gilmartin PM. 2006. Analysis of late stage flower development in Primula vulgaris reveals novel differences in cell morphology and temporal aspects of floral heteromorphy. New Phytologist, 171(3): 591-603.
- Woodell SRJ. 1960. Studies in British primulas. VII. Development of normal seed and of hybrid seed from reciprocal crosses between P. vulgaris Huds. and P. veris L. New Phytologist, 59(3): 302-313.
- Xu Y, Hao G, Hu CM. 2019. Primula vallicola sp. nov. (Primulaceae) from Sichuan, China. Nordic J Botany, 37(7): e02116.
- Yankova-Tsvetkova E, Yurukova-Grancharova P, Aneva I, Zhelev P. 2021. On the reproductive potential in primula veris L. (Primulaceae): embryological features, pollen and seed viability, genetic diversity. Plants, 10(11): 2296.
- Zhang LB, Kadereit JW. 2004. Classification of Primula sect. Auricula (Primulaceae) based on two molecular data sets (ITS, AFLPs), morphology and geographical distribution. Bot J Linn Soc, 146: 1-26.
Yıl 2022,
Cilt: 5 Sayı: 4, 464 - 469, 01.10.2022
Öz
Proje Numarası
PYO.ZRT.1901.18.004
Kaynakça
- Akhalkatsi M, Gvaladze G, Taralashvili N. 1998. Embryology of Primula algida and Primula amoena (Primulaceae). Bulletin Georgian Nat Acad Sci, 157(1): 98-100.
- Aronne G, Iovane M, Strumia S. 2021. Temperature and humidity affect pollen viability and may trigger distyly disruption in threatened species. Annali Di Botanica, 11: 77-82.
- Bateson W, Gregory RP. 1905. On the inheritance of heterostylism in Primula. Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 76(513): 581-586.
- Calahan CN, Gliddon C. 1985. Genetic neighbourhood sizes in Primula vulgaris. Heredity, 54: 65-70.
- Cocker JM, Wright J, Li J, Swarbreck D, Dyer S, Caccamo M, Gilmartin PM. 2018. Primula vulgaris (primrose) genome assembly, annotation and gene expression, with comparative genomics on the heterostyly supergene. Sci Reports, 8(1): 1-13.
- Gachechiladze MI. 1993. The embryology of Primula bayernii (Primulaceae). Bot J, 78: 93-96.
- Genç S, Soysal Mİ. 2018. Parametric and nonparametric post hoc tests. BSJ Eng Sci, 1(1): 18-27.
- Grouh MSH, Sousaraei N, Akbari M, Rahimi V, Bayat H. 2015. Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation. Turkish J Agri Forest, 39(4): 596-600.
- Gündoğan Z, Coşkunçelebi K, Gültepe M. 2019. Contribution to infra-specific taxonomy of Primula vulgaris Huds. (Primulaceae) along an altitudinal gradient in Turkey. Taiwania, 64(3): 249-257.
- Jia Y, Zhang QX, Pan HT, Wang SQ, Liu QL, Sun LX. 2014. Callus induction and haploid plant regeneration from baby primrose (Primula forbesiiFranch.) anther culture. Scientia Hort, 176: 273-281.
- Johansen DA. 1940. Plant microtechnique. McGraw-Hill Book Company Inc, London, UK, pp: 530.
- Johri BM, Ambegaokar KB, Srivastava PS. 1992. Primulales. In comparative embryology of angiosperms. Springer, Berlin, Heidelberg, Germany, pp: 629-635.
- Karabıyık Ş, Eti S. 2020. Nucellar embryony and formation mechanism in Sour Orange. Yuzuncu Yil Univ J Agri Sci, 30(4): 761-771.
- Karabıyık Ş. 2022. Effects of temperature on pollen viability and in vivo pollen tube growth in Citrus sinensis. J App Botany Food Qual, 95: 100-104.
- Keller B, Vos JM, Schmidt‐Lebuhn AN, Thomson JD, Conti E. 2016. Both morph and species dependent asymmetries affect reproductive barriers between heterostylous species. Ecol Evol, 6(17): 6223-6244.
- Li J, Webster MA, Smith MC, Gilmartin PM. 2011. Floral heteromorphy in Primula vulgaris: Progress toward sisolation and characterization of the S locus. Ann Bot, 108: 715-726.
- Li J, Webster MA, Wright J, Cocker J, Smith MC, Badakshi F, Heslop-Harrison P, Gilmartin PM. 2015. Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization. New Phytol, 208: 137-148.
- Prajapati DN, Knox JF, Emmons J, Saeian K, Csuka ME, Binion DG. 2003. Leflunomide treatment of crohn's disease patients intolerant to standard immunomodulator therapy. J Clin Gastroenterol, 37: 125-133.
- Smith WW, Forrest G. 1929. The sections of the genus Primula. J Hortic Soc, 54: 1-50.
- Tütüncü M, Mendi YY. 2020. Evaluation of pollen tube growth and fertilization via histological analysis in Cyclamen persicum. Acta Hortic, 1283: 21-26.
- Uce İ, Tunçtürk M. 2014. Hakkâri’ de doğal olarak yetişen ve yaygın olarak kullanılan bazı yabani bitkiler. Biyol Bil Araş Derg, 7(2): 21-25.
- Webster MA, Gilmartin PM. 2003. A comparison of early floral ontogeny in wild-type and floral homeotic mutant phenotypes of Primula. Planta, 216(6): 903-917.
- Webster MA, Gilmartin PM. 2006. Analysis of late stage flower development in Primula vulgaris reveals novel differences in cell morphology and temporal aspects of floral heteromorphy. New Phytologist, 171(3): 591-603.
- Woodell SRJ. 1960. Studies in British primulas. VII. Development of normal seed and of hybrid seed from reciprocal crosses between P. vulgaris Huds. and P. veris L. New Phytologist, 59(3): 302-313.
- Xu Y, Hao G, Hu CM. 2019. Primula vallicola sp. nov. (Primulaceae) from Sichuan, China. Nordic J Botany, 37(7): e02116.
- Yankova-Tsvetkova E, Yurukova-Grancharova P, Aneva I, Zhelev P. 2021. On the reproductive potential in primula veris L. (Primulaceae): embryological features, pollen and seed viability, genetic diversity. Plants, 10(11): 2296.
- Zhang LB, Kadereit JW. 2004. Classification of Primula sect. Auricula (Primulaceae) based on two molecular data sets (ITS, AFLPs), morphology and geographical distribution. Bot J Linn Soc, 146: 1-26.
Toplam 27 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Yapısal Biyoloji , Ziraat Mühendisliği |
| Bölüm | Research Articles |
| Yazarlar | |
| Proje Numarası | PYO.ZRT.1901.18.004 |
| Yayımlanma Tarihi | 1 Ekim 2022 |
| Gönderilme Tarihi | 22 Ağustos 2022 |
| Kabul Tarihi | 26 Eylül 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 5 Sayı: 4 |
