BibTex RIS Kaynak Göster

Determination of proportion of viruliferous <em>Polymyxa betae</em> Keskin in sugar beet production areas infested with <em>Beet necrotic yellow vein virus </em>and/or <em>Beet soilborne virus</em> in Samsun province

Yıl 2011, Cilt: 51 Sayı: 2, 175 - 185, 13.06.2011

Semra Saraçoğlu N. Kutluk Yılmaz

Öz

This study was conducted to determine P. betae, BNYVV and/or BSBV inoculum densities in sugar beet (Beta vulgaris L.) production areas infested with soilborne viruses in Samsun province by Most probable numbers (MPN) technique and to determine the percentages of viruliferous P. betae. For this purpose, susceptible sugar beet cultivar to BNYVV was grown in the collected soil samples and then analysed using Enzyme-linked immunosorbent assay (ELISA) for virus detection, and using light microscopy following roots staining with acid fuchsin for detection of P. betae. The result of this study showed that 0.4% and 1.2% of the infective population of P. betae were viruliferous in BNYVV infested soil samples from the Bafra and Vezirkopru districts, respectively. Also, in BSBV infested soils from Havza and Kavak districts, 9.7% and 17.3% of the infective population of P. betae were viruliferous. In mixed infested soil samples from Vezirköprü, the percentages of viruliferous P. betae were 4.8% for BNYVV and 5.7% for BSBV. In another soil with mixed virus infection from Bafra, 54.7% and 66.7% of the infective populations of P. betae were viruliferous for BNYVV and BSBV, respectively.

Anahtar Kelimeler

-

Kaynakça

  • Abe H. and Tamada T. 1986. Association of Beet Necrotic Yellow Vein Virus with Isolates of Polymyxa betae Keskin. Annals of the Phytopathological Society of Japan, 52, 235-247.
  • Abe N. 1987. Studies of the Ecology and Control of Polymyxa betae Keskin as a Fungal Vector of the Causal Virus (Beet Necrotic Yellow Vein Virus) of Rhizomania Disease of Sugar Beet. Bulletin of Hokkaido Prefectural Agricultural Experimental Station, 60, 81.
  • Adams M. and Welham S. 1995. Use of Most Probable Number Tecnique to Quantify Soil Borne Plant Pathogens. Annals of Applied Biology, 126, 181-96.
  • Anonymous, 2005. http://fao.faosted.org/site (Erişim tarihi: 25.09.2006)
  • Anonim, 2005. Samsun Tarım İl Müdürlüğü Envanterleri. Samsun.
  • Asher M. 1999. Sugar-beet Rhizomania: The Spread of a Soilborne Disease. Microbiology Today. 26, 120-122.
  • Asher M.J.C. and Blunt S.J. 1987. The Ecological Requirements of Polymyxa betae. Proceeding of the 50th Winter Congress of the Int. Inst. Sugar Beet Res., 11-12 February 1987, Brussels, 45-55.
  • Buttner G., Marlander B. and Manthey R. 1995. Breeding for Resistance to Rhizomania in Sugar Beet (Beta vulgaris L.). Plant Breeding, 114, 160-164.
  • Ciafardini G. and Marotta B. 1989. Use of Most Probably Number Tecnique to Detect Polymyxa betae (Plasmodiophoromycetes) in Soil. Applied and Environmental Microbiology, 55, 1273-1278.
  • Ciafardini G. 1991. Evaluation of Polymyxa betae Keskin Contaminated by Beet necrotic yellow vein virus in Soil. Applied and Environmental Microbiology, 57, 1817-1821.
  • Ertunç F. 1998. Polymyxa betae (Keskin)‟nın Şeker Pancarı Kılcal Köklerindeki Biyolojik Dönemleri Üzerinde Araştırmalar. A. Ü. Ziraat Fak. Yayınları, No: 1495, Ankara, 17s.
  • Fujisawa I. and Sugimoto T. 1977. Transmission of beet necrotic yellow vein virus by Polymyxa betae. Annals of Phytopathological Society of Japan, 43, 583-586.
  • Giunchedi L., De Biaggi M. and Pollini C. 1987. Correlation between Tolerance and Beet necrotic yellow vein virus in Sugar Beet Genotypes. Phytopathologia Mediterranea, 26, 23-28.
  • Henry C. 1996. Rhizomania- Its Effect on Sugar Beet Yield in the UK. British Sugar Beet Review, 64 (2), 24-26.
  • Hurley M.A. and Roscoe M.E. 1983. Automated Statistical Analysis of Microbial Enumeration by Dilution Series. Journal of Applied Bacteriology, 55, 159-164.
  • Ivanoviç M., Macfarlane I. and Woods R.D. 1983. Viruses of Sugarbeet Associated with Polymyxa betae. Annual Report Rothamsted Experimental Station, 1982, 189-190.
  • Kastirr U., Pfeilstetter E. and Burgermeister W. 1994. Virus Content and Virulence of Polymyxa betae Keskin Isolates Obtained from Different Regions in Europe. Journal of Phytopathology, 141, 369-374.
  • Keskin B. 1964. Polmyxa betae n.sp. ein parasit in den wurzeln von Beta vulgaris Tournefort, besonders wahrend den jugendent wicklung den zuckerrübe. Archives of Microbiology, 49, 348-374.
  • Koenig R., Pleij C.W.A., Beier C. and Commandeur U. 1998. Genome Properties of Beet virus Q, a New Furo-like Virus from Sugar Beet, Determined from Unpurified Virus. Journal of General Virology, 79, 2027-2036.
  • Kutluk Yilmaz N.D., Saracoglu S., Sevik M.A. and Sokmen M. 2005. A Survey on the Incidences of Beet necrotic yellow vein virus (BNYVV), Beet soilborne virus (BSBV) and Their Vector, Polymyxa betae Keskin in Sugar Beet Fields in Northern and Central Parts of Turkey. Proceedings of the Sixth Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 5-7 September 2005, Bologna, Italy, 141-145.
  • Legreve A., Schmit J.F., Bragard C. and Maraite H. 2005. The Role of Climate and Alternative Hosts in the Epidemiology of Rhizomania. Proceedings of the Sixth Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 5-7 September 2005, Bologna, Italy, 125-128.
  • Meunier A., Schmit J-F., Stas A., Kutluk N. and Bragard C. 2003. Multiplex Reverse Transcription for Simultaneous Detection of Beet Necrotic Yellow Vein Virus, Beet Soilborne Virus, and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet. Applied and Environmental Microbiology, 69 (4), 2356-2360.
  • Mouhanna A.M., Nasrallah A., Langen G. and Schlösser E. 2002. Surveys for Beet necrotic yellow vein virus (the Causes Rhizomania), Other Viruses, and Soil-borne Fungi Infected Sugar Beet in Syria. Journal of Phytopathology, 150, 657-662.
  • Prillwitz H. and Schlösser E. 1992. Beet Soil-borne Virus: Occurrence, Symptoms and Effect on Plant Development. Medical Faculty Landbouw, University Gent, 57 (2a), 295-302.
  • Rush C.M. 2003. Ecology and Epidemiolgy of Benyviruses and Plasmodiophorid Vectors. Annual Review of Phytopathology, 41, 567-592.
  • Rysanek P., Stocky G., Haeberle A.M. and Putz C. 1992. Immunogold Labelling of beet necrotic yellow vein virus Particle Inside its Fungal Vector, Polymyxa betae K. Agronomie, 12, 651-659.
  • Saraçoğlu, S. 2007. Samsun Ili Şeker Pancarı Üretim Alanlarında Vektör Polmyxa betae Keskin ile Taşınan Toprak Kökenli Virüs Hastalıklarının Belirlenmesi. Yüksek Lisans tezi, O.M.U Fen Bilimleri Enstitüsü, Samsun, 89 s.
  • Schlösser, E. 1988. Epidemiology and manegement of Polymyxa betae and Beet necrotic yellow vein virus. Viruses with Fungal Vectors, Develops in Applied Biology II., Association of Applied Biologist, Wellesborne, England, 281-292.
  • Stas A., Meunier A., Schmit J.-F., and Bragard C. 2001. First report of Beet virus Q in Belgium. Plant Disease, 85 (12), 1288.
  • Tamada T. 1975. Beet Necrotic Yellow Vein Virus. CMI/ABB. Descriptions of Plant Viruses. No: 144.
  • Tamada T. 1999. Benyviruses. In: Webster R.G. and Granoff A. (eds). Encyclopedia of Virology, Second Edition. pp. 154-160. Academic Press, London, England.
  • Tuitert G. 1990. Assessment of the Inoculum Potential of Polymyxa betae and Beet necrotic yellow vein virus in Soil Using the Most Probable Number Method. Netherlands Journal of Plant Pathology, 96, 331-341.
  • Tuitert G. 1994. Epidemiology of Rhizomania Disease of Sugarbeet. PhD thesis, Wageningen Agricultural University, the Netherlands, 168 p.
  • Tuitert G. and Hofmeester Y. 1992. Epidemiology of Beet necrotic yellow vein virus in Sugar Beet at Different Initial Inoculum Levels in the Presence or Absence of Irrigation; Dynamics of Inoculum. Netherlands Journal of Plant Pathology, 98, 343- 360.
  • Turina M., Resca R. and Rubies-Autonell C. 1996. Surveys of Soilborne Virus Diseases of Sugar Beet in Italy. Proceedings of the Third Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 6-7 August 1996, Dundee, Scotland, 121-124.
  • Whitney E.D. and Duffus J.E. 1995. Compendium of Beet Diseases and Insects. The American Phytopathological Society Press, Minnesota, USA, 76 p.
  • Wisler G.C., Liu H.Y. and Duffus J. E. 1994. Beet Necrotic Yellow Vein Virus and Its Relationship to Eight Sugar Beet Furo-Like Viruses from the United States. Plant. Disease, 78 (10), 995-1001.
  • Wisler G.C, Lewellen R.T, Sears J.L., Wasson J.W, Liu H-Y. and Wintermantel W.M. 2003. Interaction between Beet necrotic yellow vein virus and Beet soilborne mosaic virus in Sugar Beet. Plant Disease, 87 (10), 1170-1175.

Samsun ilinde Beet necrotic yellow vein virus ve/veya Beet soilborne virus ile bulaşık şeker pancarı üretim alanlarında virüs taşıyan Polymyxa betae Keskin oranının belirlenmesi

Yıl 2011, Cilt: 51 Sayı: 2, 175 - 185, 13.06.2011

Semra Saraçoğlu N. Kutluk Yılmaz

Öz

Bu çalışma, Samsun ili şeker pancarı (Beta vulgaris L.) üretim alanlarına ait toprak örneklerinde ‘En Yüksek Olası Sayı' (Most probable number: MPN) tekniği ile Beet necrotic yellow vein virus (BNYVV) ve/veya Beet soilborne virus (BSBV) ile bu virüslerin vektörü olan Polymyxa betae'nın inokulum yoğunluklarının belirlenmesi ve viruliferous (virüs içeren) P. betae oranlarının ortaya konulması amacıyla yürütülmüştür. Bu amaçla, BNYVV'ye hassas şeker pancarı çeşidi bölgeden seçilen topraklara ekilmiş ve sonrasında virüslerin tespiti için ELISA ve P. betae için de asit fuksin ile kök boyamasını takiben ışık mikroskobu kullanılarak analizler yapılmıştır. Araştırma sonucunda; BNYVV ile bulaşık Bafra ve Vezirköprü toprak örneklerinde viruliferous P. betae oranları %0.4 ile %1.2 olarak belirlenirken, BSBV ile bulaşık Havza ve Kavak ilçelerinde ise bu oranın %9.7 ile %17.3 olduğu tespit edilmiştir. Bununla birlikte, her iki virüs ile bulaşık olan Vezirköprü ilçesi örneğinde virüs içeren P. betae'ların %4.8'inin BNYVV, %5.7'sinin ise BSBV ile bulaşık olduğu saptanırken; Bafra toprak örneğinde bu oranın BNYVV için %54.7, BSBV için %66.7 olduğu belirlenmiştir.

Anahtar Kelimeler

BSBV MPN Polymyxa betae rhizomania sugar beet şeker pancarı

Kaynakça

  • Abe H. and Tamada T. 1986. Association of Beet Necrotic Yellow Vein Virus with Isolates of Polymyxa betae Keskin. Annals of the Phytopathological Society of Japan, 52, 235-247.
  • Abe N. 1987. Studies of the Ecology and Control of Polymyxa betae Keskin as a Fungal Vector of the Causal Virus (Beet Necrotic Yellow Vein Virus) of Rhizomania Disease of Sugar Beet. Bulletin of Hokkaido Prefectural Agricultural Experimental Station, 60, 81.
  • Adams M. and Welham S. 1995. Use of Most Probable Number Tecnique to Quantify Soil Borne Plant Pathogens. Annals of Applied Biology, 126, 181-96.
  • Anonymous, 2005. http://fao.faosted.org/site (Erişim tarihi: 25.09.2006)
  • Anonim, 2005. Samsun Tarım İl Müdürlüğü Envanterleri. Samsun.
  • Asher M. 1999. Sugar-beet Rhizomania: The Spread of a Soilborne Disease. Microbiology Today. 26, 120-122.
  • Asher M.J.C. and Blunt S.J. 1987. The Ecological Requirements of Polymyxa betae. Proceeding of the 50th Winter Congress of the Int. Inst. Sugar Beet Res., 11-12 February 1987, Brussels, 45-55.
  • Buttner G., Marlander B. and Manthey R. 1995. Breeding for Resistance to Rhizomania in Sugar Beet (Beta vulgaris L.). Plant Breeding, 114, 160-164.
  • Ciafardini G. and Marotta B. 1989. Use of Most Probably Number Tecnique to Detect Polymyxa betae (Plasmodiophoromycetes) in Soil. Applied and Environmental Microbiology, 55, 1273-1278.
  • Ciafardini G. 1991. Evaluation of Polymyxa betae Keskin Contaminated by Beet necrotic yellow vein virus in Soil. Applied and Environmental Microbiology, 57, 1817-1821.
  • Ertunç F. 1998. Polymyxa betae (Keskin)‟nın Şeker Pancarı Kılcal Köklerindeki Biyolojik Dönemleri Üzerinde Araştırmalar. A. Ü. Ziraat Fak. Yayınları, No: 1495, Ankara, 17s.
  • Fujisawa I. and Sugimoto T. 1977. Transmission of beet necrotic yellow vein virus by Polymyxa betae. Annals of Phytopathological Society of Japan, 43, 583-586.
  • Giunchedi L., De Biaggi M. and Pollini C. 1987. Correlation between Tolerance and Beet necrotic yellow vein virus in Sugar Beet Genotypes. Phytopathologia Mediterranea, 26, 23-28.
  • Henry C. 1996. Rhizomania- Its Effect on Sugar Beet Yield in the UK. British Sugar Beet Review, 64 (2), 24-26.
  • Hurley M.A. and Roscoe M.E. 1983. Automated Statistical Analysis of Microbial Enumeration by Dilution Series. Journal of Applied Bacteriology, 55, 159-164.
  • Ivanoviç M., Macfarlane I. and Woods R.D. 1983. Viruses of Sugarbeet Associated with Polymyxa betae. Annual Report Rothamsted Experimental Station, 1982, 189-190.
  • Kastirr U., Pfeilstetter E. and Burgermeister W. 1994. Virus Content and Virulence of Polymyxa betae Keskin Isolates Obtained from Different Regions in Europe. Journal of Phytopathology, 141, 369-374.
  • Keskin B. 1964. Polmyxa betae n.sp. ein parasit in den wurzeln von Beta vulgaris Tournefort, besonders wahrend den jugendent wicklung den zuckerrübe. Archives of Microbiology, 49, 348-374.
  • Koenig R., Pleij C.W.A., Beier C. and Commandeur U. 1998. Genome Properties of Beet virus Q, a New Furo-like Virus from Sugar Beet, Determined from Unpurified Virus. Journal of General Virology, 79, 2027-2036.
  • Kutluk Yilmaz N.D., Saracoglu S., Sevik M.A. and Sokmen M. 2005. A Survey on the Incidences of Beet necrotic yellow vein virus (BNYVV), Beet soilborne virus (BSBV) and Their Vector, Polymyxa betae Keskin in Sugar Beet Fields in Northern and Central Parts of Turkey. Proceedings of the Sixth Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 5-7 September 2005, Bologna, Italy, 141-145.
  • Legreve A., Schmit J.F., Bragard C. and Maraite H. 2005. The Role of Climate and Alternative Hosts in the Epidemiology of Rhizomania. Proceedings of the Sixth Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 5-7 September 2005, Bologna, Italy, 125-128.
  • Meunier A., Schmit J-F., Stas A., Kutluk N. and Bragard C. 2003. Multiplex Reverse Transcription for Simultaneous Detection of Beet Necrotic Yellow Vein Virus, Beet Soilborne Virus, and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet. Applied and Environmental Microbiology, 69 (4), 2356-2360.
  • Mouhanna A.M., Nasrallah A., Langen G. and Schlösser E. 2002. Surveys for Beet necrotic yellow vein virus (the Causes Rhizomania), Other Viruses, and Soil-borne Fungi Infected Sugar Beet in Syria. Journal of Phytopathology, 150, 657-662.
  • Prillwitz H. and Schlösser E. 1992. Beet Soil-borne Virus: Occurrence, Symptoms and Effect on Plant Development. Medical Faculty Landbouw, University Gent, 57 (2a), 295-302.
  • Rush C.M. 2003. Ecology and Epidemiolgy of Benyviruses and Plasmodiophorid Vectors. Annual Review of Phytopathology, 41, 567-592.
  • Rysanek P., Stocky G., Haeberle A.M. and Putz C. 1992. Immunogold Labelling of beet necrotic yellow vein virus Particle Inside its Fungal Vector, Polymyxa betae K. Agronomie, 12, 651-659.
  • Saraçoğlu, S. 2007. Samsun Ili Şeker Pancarı Üretim Alanlarında Vektör Polmyxa betae Keskin ile Taşınan Toprak Kökenli Virüs Hastalıklarının Belirlenmesi. Yüksek Lisans tezi, O.M.U Fen Bilimleri Enstitüsü, Samsun, 89 s.
  • Schlösser, E. 1988. Epidemiology and manegement of Polymyxa betae and Beet necrotic yellow vein virus. Viruses with Fungal Vectors, Develops in Applied Biology II., Association of Applied Biologist, Wellesborne, England, 281-292.
  • Stas A., Meunier A., Schmit J.-F., and Bragard C. 2001. First report of Beet virus Q in Belgium. Plant Disease, 85 (12), 1288.
  • Tamada T. 1975. Beet Necrotic Yellow Vein Virus. CMI/ABB. Descriptions of Plant Viruses. No: 144.
  • Tamada T. 1999. Benyviruses. In: Webster R.G. and Granoff A. (eds). Encyclopedia of Virology, Second Edition. pp. 154-160. Academic Press, London, England.
  • Tuitert G. 1990. Assessment of the Inoculum Potential of Polymyxa betae and Beet necrotic yellow vein virus in Soil Using the Most Probable Number Method. Netherlands Journal of Plant Pathology, 96, 331-341.
  • Tuitert G. 1994. Epidemiology of Rhizomania Disease of Sugarbeet. PhD thesis, Wageningen Agricultural University, the Netherlands, 168 p.
  • Tuitert G. and Hofmeester Y. 1992. Epidemiology of Beet necrotic yellow vein virus in Sugar Beet at Different Initial Inoculum Levels in the Presence or Absence of Irrigation; Dynamics of Inoculum. Netherlands Journal of Plant Pathology, 98, 343- 360.
  • Turina M., Resca R. and Rubies-Autonell C. 1996. Surveys of Soilborne Virus Diseases of Sugar Beet in Italy. Proceedings of the Third Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 6-7 August 1996, Dundee, Scotland, 121-124.
  • Whitney E.D. and Duffus J.E. 1995. Compendium of Beet Diseases and Insects. The American Phytopathological Society Press, Minnesota, USA, 76 p.
  • Wisler G.C., Liu H.Y. and Duffus J. E. 1994. Beet Necrotic Yellow Vein Virus and Its Relationship to Eight Sugar Beet Furo-Like Viruses from the United States. Plant. Disease, 78 (10), 995-1001.
  • Wisler G.C, Lewellen R.T, Sears J.L., Wasson J.W, Liu H-Y. and Wintermantel W.M. 2003. Interaction between Beet necrotic yellow vein virus and Beet soilborne mosaic virus in Sugar Beet. Plant Disease, 87 (10), 1170-1175.
Toplam 38 adet kaynakça vardır.

Ayrıntılar

Birincil Dil TR
Bölüm Makaleler
Yazarlar

Semra Saraçoğlu Bu kişi benim

N. Kutluk Yılmaz

Yayımlanma Tarihi 13 Haziran 2011
Gönderilme Tarihi 13 Haziran 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 51 Sayı: 2

Kaynak Göster

APA Saraçoğlu, S., & Kutluk Yılmaz, N. (2011). Determination of proportion of viruliferous Polymyxa betae Keskin in sugar beet production areas infested with Beet necrotic yellow vein virus and/or Beet soilborne virus in Samsun province. Plant Protection Bulletin, 51(2), 175-185.
AMA Saraçoğlu S, Kutluk Yılmaz N. Determination of proportion of viruliferous Polymyxa betae Keskin in sugar beet production areas infested with Beet necrotic yellow vein virus and/or Beet soilborne virus in Samsun province. Plant Protection Bulletin. Haziran 2011;51(2):175-185.
Chicago Saraçoğlu, Semra, ve N. Kutluk Yılmaz. “Determination of Proportion of Viruliferous Polymyxa betae Keskin in Sugar Beet Production Areas Infested With Beet Necrotic Yellow Vein Virus and/or Beet Soilborne virus In Samsun Province”. Plant Protection Bulletin 51, sy. 2 (Haziran 2011): 175-85.
EndNote Saraçoğlu S, Kutluk Yılmaz N (01 Haziran 2011) Determination of proportion of viruliferous Polymyxa betae Keskin in sugar beet production areas infested with Beet necrotic yellow vein virus and/or Beet soilborne virus in Samsun province. Plant Protection Bulletin 51 2 175–185.
IEEE S. Saraçoğlu ve N. Kutluk Yılmaz, “Determination of proportion of viruliferous Polymyxa betae Keskin in sugar beet production areas infested with Beet necrotic yellow vein virus and/or Beet soilborne virus in Samsun province”, Plant Protection Bulletin, c. 51, sy. 2, ss. 175–185, 2011.
ISNAD Saraçoğlu, Semra - Kutluk Yılmaz, N. “Determination of Proportion of Viruliferous Polymyxa betae Keskin in Sugar Beet Production Areas Infested With Beet Necrotic Yellow Vein Virus and/or Beet Soilborne virus In Samsun Province”. Plant Protection Bulletin 51/2 (Haziran 2011), 175-185.
JAMA Saraçoğlu S, Kutluk Yılmaz N. Determination of proportion of viruliferous Polymyxa betae Keskin in sugar beet production areas infested with Beet necrotic yellow vein virus and/or Beet soilborne virus in Samsun province. Plant Protection Bulletin. 2011;51:175–185.
MLA Saraçoğlu, Semra ve N. Kutluk Yılmaz. “Determination of Proportion of Viruliferous Polymyxa betae Keskin in Sugar Beet Production Areas Infested With Beet Necrotic Yellow Vein Virus and/or Beet Soilborne virus In Samsun Province”. Plant Protection Bulletin, c. 51, sy. 2, 2011, ss. 175-8.
Vancouver Saraçoğlu S, Kutluk Yılmaz N. Determination of proportion of viruliferous Polymyxa betae Keskin in sugar beet production areas infested with Beet necrotic yellow vein virus and/or Beet soilborne virus in Samsun province. Plant Protection Bulletin. 2011;51(2):175-8.

134351364813650136491344113445