Araştırma Makalesi
Yıl 2023,
Cilt: 4 Sayı: 1, 1 - 6, 26.06.2023
Öz
Kaynakça
- Abd El-Hafeez AA, Khalifa HO, Mahdy EAM, Sharma V, Hosoi T, Ghosh P, Ozawa K, Montano MM, Fujimura T, Ibrahim ARN, Abdelhamid MAA, Pack SP, Shouman SA, Kawamoto S (2019) Anticancer effect of nor-wogonin (5, 7, 8-trihydroxyflavone) on human triple-negative breast cancer cells via downregulation of TAK1, NF-κB, and STAT3. Pharmacol Rep 71(2):289-298
- Aggarwal BB, Shishodia S, Takada Y, Banerjee S, Newman RA, Bueso-Ramos CE, Price JE (2005) Curcumin suppresses the paclitaxel-induced nuclear factor-kappaB pathway in breast cancer cells and inhibits lung metastasis of human breast cancer in nude mice. Clin Cancer Res 11:7490-7498
- Barsouk A, Padala SA, Vakiti A, Mohammed A, Saginala K, Thandra KC, Rawla P, Barsouk A (2020) Epidemiology, Staging and Management of Prostate Cancer. Medical Sciences 8(3):28
- Cadona FC, Dantas RF, de Mello GH, Silva-Jr FP (2022). Natural products targeting into cancer hallmarks: An update on caffeine, theobromine, and (+)-catechin. Critical Reviews in Food Science and Nutrition 62(26):7222-7241
- Chen D, Dou QP (2008) Tea polyphenols and their roles in cancer prevention and chemotherapy. Int J Mol Sci 9 (7):1196- 1206
- Chow JM, Huang GC, Shen SC, Wu CY, Lin CW, Chen YC (2008) Differential apoptotic effect of wogonin and nor-wogonin via stimulation of ROS production in human leukemia cells. J Cell Biochem 103:1394–1404
- Denis L, Morton MS, Griffiths K (1999) Diet and its preventive role in prostatic disease. Eur Urol 35, 377–387 Eastman A, Perez RP (2006) New targets and challenges in the molecular therapeutics of cancer. Br J Clin Pharmacol 62(1):5-14
- Estaquier J, Vallette F, Vayssiere JL, Mignotte B (2012) The mitochondrial pathways of apoptosis. Adv Exp Med Biol 942:157-83
- Farnebo M, Bykov VJ, Wiman KG (2010) The p53 tumor suppressor: a master regulator of diverse cellular processes and therapeutic target in cancer. Biochem Biophys Res Commun 396:85-89
- Genc M, Karagoz Genc Z, Tekin S, Sandal S, Sirajuddin M, Hadda Taibi B, Sekerci M (2016) Design, Synthesis, in vitro Antiproliferative Activity, Binding Modeling of 1,2,4,-Triazoles as New Anti-Breast Cancer Agents. Acta Chim Slov 63(4):726-737
- Havsteen BH (2002) The biochemistry and medical significance of the flavonoids. Pharmacology & therapeutics 96(2-3):67-202
- Huang GC, Chow JM, Shen SC, Yang LY, Lin CW, Chen, YC (2007) Wogonin but not Nor-wogonin inhibits lipopolysaccharide and lipoteichoic acid-induced iNOS gene expression and NO production in macrophages. Int Immunopharmacol 7(8):1054-1063
- Huang YT, Chueh SC, Teng CM, Guh JH (2004) Investigation of ouabain-induced anticancer effect in human androgen-independent prostate cancer PC-3 cells. Biochem Pharmacol 15;67(4):727-33
- Hui KM, Huen MS, Wang HY, Zheng H, Sigel E, Baur R, Xue H (2002) Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi. Biochemical pharmacology 64(9):1415-1424
- Jing L, Gao R, Zhang J, Zhang D, Shao J, Jia Z, Ma H (2021) Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells. BMC Complementary Medicine and Therapies 21(1):1-12
- Karakuş A, Karakuş SÜ (2022) Nor-wogonin’in İnsan Rahim Ağzı Kanseri Hücrelerinde Antikanser ve Apoptotik Etkileri. Avrupa Bilim ve Teknoloji Dergisi (34):617-622
- Kerr JF, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26(4):239-257
- Klocker H, Culig Z, Eder IE, Nessler-Menardi C, Hobisch A, et al (1999) Mechanism of androgen receptor activation and possible implications for chemoprevention trials. Eur Urol 35:413-419 Koran K, Tekin Ç, Çalışkan E, Tekin S, Sandal S, Görgülü AO (2017) Synthesis, structural and thermal characterizations and in vitro cytotoxic activities of new cyclotriphosphazene derivatives. Phosphorus Sulfur Silicon Relat Elem 192(9):1002-1011
- Li JW, Vederas JC (2011) Drug discovery and natural products: end of era or an endless frontier? Biomed Khim 57(2):148-160
- Liu J, Huang R, Lin D, Peng J, Wu X, Lin Q (2005) Expression of survivin and bax/bcl-2 in peroxisome proliferator activated receptor-γ ligands induces apoptosis on human myeloid leukemia cells in vitro. Ann Oncol 16:455-459
- Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25(4):402-408
- Miyasaki Y, Rabenstein JD, Rhea J, Crouch ML, Mocek UM, Kittell PE, Liu GY (2013) Isolation and characterization of antimicrobial compounds in plant extracts against multidrug-resistant Acinetobacter baumannii. PloS one 8(4):e61594
- Miyashita T, Krajewski S, Krajewska M, Wang HG, Lin H, Liebermann DA (1994) Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene 9:1799-1805
- Mondal S, Bandyopadhyay S, Ghosh M, Mukhopadhyay S, Roy S, Mandal C (2011) Natural Products: Promising Resources For Cancer Drug Discovery. Anticancer Agents Med Chem 12(1):49-75
- Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65 (1):55-63
- Nishikawa T, Nakajima T, Moriguchi M, Jo M, Sekoguchi S, Ishii M, Takashima H, Katagishi T, Kimura H, Minami M, Itoh Y, Kagawa K, Okanoue T (2006) A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. J Hepatol 44:1074–1082
- Pallauf K, Duckstein N, Rimbach GA (2017) Literature review of flavonoids and lifespan in model organisms. Proc Nutr Soc 76:145–162
- Panvichian R and Pienta KJ (1996) Hormonal and chemotherapeutic systemic therapy for metastatic prostate cancer. Cancer Control 3:493–500
- Ravishankar D, Rajora AK, Greco F, Osborn HMI (2013) Flavonoids as prospective compounds for anti-cancer therapy. Int. J Biochem Cell Biol 45:2821–2831
- Russell PJ, Bennett S, Stricker P (1998) Growth factor involvement in progression of prostate cancer. Clin Chem 44:705-723
- S Patel P, Joshee N, M Rimando A, Parajuli P (2013) Anti-cancer scopes and associated mechanisms of Scutellaria extract and flavonoid wogonin. Curr Cancer Ther Rev 9(1):34-42
- Sahin O, Ozdemir UO, Seferoglu N, Genc ZK, Kaya K, Aydiner B, Tekin S, Seferoglu Z (2018) New platinum (II) and palladium (II) complexes of coumarin-thiazole Schiff base with a fluorescent chemosensor properties: Synthesis, spectroscopic characterization, X-ray structure determination, in vitro anticancer activity on various human carcinoma cell lines and computational studies. J Photochem. Photobiol B 178:428-439
- Shu L, Cheung KL, Khor TO, Chen C, Kong AN (2010) Phytochemicals: cancer chemoprevention and suppression of tumor onset and metastasis. Cancer Metastasis Rev 29 (3):483-502
- Srivastava S, Somasagara RR, Hegde M, Nishana M, Tadi SK, Srivastava M, Choudhary B, Raghavan SC (2016) Quercetin, a Natural Flavonoid Interacts with DNA, Arrests Cell Cycle and Causes Tumor Regression by Activating Mitochondrial Pathway of Apoptosis Sci Rep (12)6:24049
- Sultana N (2011) Clinically useful anticancer, antitumor, and antiwrinkle agent, ursolic acid and related derivatives as medicinally important natural product. J Enzyme Inhib Med Chem 0:1-27
- Takada Y, Murakami A, Aggarwal BB (2005) Zerumbone abolishes NF-kappaB and IkappaBalpha kinase activation leading to suppression of antiapoptotic and metastatic gene expression, upregulation of apoptosis, and downregulation of invasion. Oncogene 24:6957–6969
- Tang DG and Porter AT (1997) Target to apoptosis: a hopeful weapon for prostate cancer. Prostate 32:284-93
- Toshiya K, Testuya T, Akira H, Takuji T (2012) Cancer chemoprevention through the induction of apoptosis by natural compounds. Biophys Chem 3(2):156-173
- Wang Z, Zhang Q, Zhou L, Liu G, Wu Q, Chen C (2020) Nor-wogonin flavone suppresses the growth of human colon cancer cells via mitochondrial mediated apoptosis, autophagy induction and triggering G2/M phase cell cycle arrest. J Buon 25(3):1449-1454
5,7,8-trihydroxyflavone Has Anticancer and Apoptotic Effects in Human Androgen-Independet Prostate Cancer PC-3 Cells
Öz
Natural products have been studied to provide alternative treatments against human diseases as they have various medicinal properties. One of these natural products is substances in the class of flavonoids. These bioactive molecules have antioxidant, anti-inflammatory and antitumor activities. The number of studies focusing on these molecules is increasing to discover new therapeutic agents against diseases such as cancer. We aimed to determine in-vitro the anticancer and apoptotic effects of 5,7,8-trihydroxyflavone (Nor-wogonin) flavone, which is one of these bioactive molecules and has various biological properties, on androgen-independet human prostate cancer (PC-3) cells. Nor-wogonin concentrations of 10, 20, 40, 80 µM were prepared and applied to human prostate cancer cells for 24 hours. The anticancer effect of flavone was determined by MTT 3-(4,5-dimethylthiazol-2-yl)-diphenyl tetrazolium bromide) method, and its effect on pro-poptotic and anti-apoptotic genes was determined by Real-Time PCR analysis. According to the obtained data, Nor-wogonin applied to PC-3 cells decreased in-vitro cell viability due to increasing concentration (p<0.05) and the IC50 value was calculated as 57.29 μM. In addition, it was determined that Nor-wogonin directed PC-3 cells to apoptosis by acting on various anti-apoptotic and pro-apoptotic gene expressions.
Anahtar Kelimeler
Kaynakça
- Abd El-Hafeez AA, Khalifa HO, Mahdy EAM, Sharma V, Hosoi T, Ghosh P, Ozawa K, Montano MM, Fujimura T, Ibrahim ARN, Abdelhamid MAA, Pack SP, Shouman SA, Kawamoto S (2019) Anticancer effect of nor-wogonin (5, 7, 8-trihydroxyflavone) on human triple-negative breast cancer cells via downregulation of TAK1, NF-κB, and STAT3. Pharmacol Rep 71(2):289-298
- Aggarwal BB, Shishodia S, Takada Y, Banerjee S, Newman RA, Bueso-Ramos CE, Price JE (2005) Curcumin suppresses the paclitaxel-induced nuclear factor-kappaB pathway in breast cancer cells and inhibits lung metastasis of human breast cancer in nude mice. Clin Cancer Res 11:7490-7498
- Barsouk A, Padala SA, Vakiti A, Mohammed A, Saginala K, Thandra KC, Rawla P, Barsouk A (2020) Epidemiology, Staging and Management of Prostate Cancer. Medical Sciences 8(3):28
- Cadona FC, Dantas RF, de Mello GH, Silva-Jr FP (2022). Natural products targeting into cancer hallmarks: An update on caffeine, theobromine, and (+)-catechin. Critical Reviews in Food Science and Nutrition 62(26):7222-7241
- Chen D, Dou QP (2008) Tea polyphenols and their roles in cancer prevention and chemotherapy. Int J Mol Sci 9 (7):1196- 1206
- Chow JM, Huang GC, Shen SC, Wu CY, Lin CW, Chen YC (2008) Differential apoptotic effect of wogonin and nor-wogonin via stimulation of ROS production in human leukemia cells. J Cell Biochem 103:1394–1404
- Denis L, Morton MS, Griffiths K (1999) Diet and its preventive role in prostatic disease. Eur Urol 35, 377–387 Eastman A, Perez RP (2006) New targets and challenges in the molecular therapeutics of cancer. Br J Clin Pharmacol 62(1):5-14
- Estaquier J, Vallette F, Vayssiere JL, Mignotte B (2012) The mitochondrial pathways of apoptosis. Adv Exp Med Biol 942:157-83
- Farnebo M, Bykov VJ, Wiman KG (2010) The p53 tumor suppressor: a master regulator of diverse cellular processes and therapeutic target in cancer. Biochem Biophys Res Commun 396:85-89
- Genc M, Karagoz Genc Z, Tekin S, Sandal S, Sirajuddin M, Hadda Taibi B, Sekerci M (2016) Design, Synthesis, in vitro Antiproliferative Activity, Binding Modeling of 1,2,4,-Triazoles as New Anti-Breast Cancer Agents. Acta Chim Slov 63(4):726-737
- Havsteen BH (2002) The biochemistry and medical significance of the flavonoids. Pharmacology & therapeutics 96(2-3):67-202
- Huang GC, Chow JM, Shen SC, Yang LY, Lin CW, Chen, YC (2007) Wogonin but not Nor-wogonin inhibits lipopolysaccharide and lipoteichoic acid-induced iNOS gene expression and NO production in macrophages. Int Immunopharmacol 7(8):1054-1063
- Huang YT, Chueh SC, Teng CM, Guh JH (2004) Investigation of ouabain-induced anticancer effect in human androgen-independent prostate cancer PC-3 cells. Biochem Pharmacol 15;67(4):727-33
- Hui KM, Huen MS, Wang HY, Zheng H, Sigel E, Baur R, Xue H (2002) Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi. Biochemical pharmacology 64(9):1415-1424
- Jing L, Gao R, Zhang J, Zhang D, Shao J, Jia Z, Ma H (2021) Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells. BMC Complementary Medicine and Therapies 21(1):1-12
- Karakuş A, Karakuş SÜ (2022) Nor-wogonin’in İnsan Rahim Ağzı Kanseri Hücrelerinde Antikanser ve Apoptotik Etkileri. Avrupa Bilim ve Teknoloji Dergisi (34):617-622
- Kerr JF, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26(4):239-257
- Klocker H, Culig Z, Eder IE, Nessler-Menardi C, Hobisch A, et al (1999) Mechanism of androgen receptor activation and possible implications for chemoprevention trials. Eur Urol 35:413-419 Koran K, Tekin Ç, Çalışkan E, Tekin S, Sandal S, Görgülü AO (2017) Synthesis, structural and thermal characterizations and in vitro cytotoxic activities of new cyclotriphosphazene derivatives. Phosphorus Sulfur Silicon Relat Elem 192(9):1002-1011
- Li JW, Vederas JC (2011) Drug discovery and natural products: end of era or an endless frontier? Biomed Khim 57(2):148-160
- Liu J, Huang R, Lin D, Peng J, Wu X, Lin Q (2005) Expression of survivin and bax/bcl-2 in peroxisome proliferator activated receptor-γ ligands induces apoptosis on human myeloid leukemia cells in vitro. Ann Oncol 16:455-459
- Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25(4):402-408
- Miyasaki Y, Rabenstein JD, Rhea J, Crouch ML, Mocek UM, Kittell PE, Liu GY (2013) Isolation and characterization of antimicrobial compounds in plant extracts against multidrug-resistant Acinetobacter baumannii. PloS one 8(4):e61594
- Miyashita T, Krajewski S, Krajewska M, Wang HG, Lin H, Liebermann DA (1994) Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene 9:1799-1805
- Mondal S, Bandyopadhyay S, Ghosh M, Mukhopadhyay S, Roy S, Mandal C (2011) Natural Products: Promising Resources For Cancer Drug Discovery. Anticancer Agents Med Chem 12(1):49-75
- Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65 (1):55-63
- Nishikawa T, Nakajima T, Moriguchi M, Jo M, Sekoguchi S, Ishii M, Takashima H, Katagishi T, Kimura H, Minami M, Itoh Y, Kagawa K, Okanoue T (2006) A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. J Hepatol 44:1074–1082
- Pallauf K, Duckstein N, Rimbach GA (2017) Literature review of flavonoids and lifespan in model organisms. Proc Nutr Soc 76:145–162
- Panvichian R and Pienta KJ (1996) Hormonal and chemotherapeutic systemic therapy for metastatic prostate cancer. Cancer Control 3:493–500
- Ravishankar D, Rajora AK, Greco F, Osborn HMI (2013) Flavonoids as prospective compounds for anti-cancer therapy. Int. J Biochem Cell Biol 45:2821–2831
- Russell PJ, Bennett S, Stricker P (1998) Growth factor involvement in progression of prostate cancer. Clin Chem 44:705-723
- S Patel P, Joshee N, M Rimando A, Parajuli P (2013) Anti-cancer scopes and associated mechanisms of Scutellaria extract and flavonoid wogonin. Curr Cancer Ther Rev 9(1):34-42
- Sahin O, Ozdemir UO, Seferoglu N, Genc ZK, Kaya K, Aydiner B, Tekin S, Seferoglu Z (2018) New platinum (II) and palladium (II) complexes of coumarin-thiazole Schiff base with a fluorescent chemosensor properties: Synthesis, spectroscopic characterization, X-ray structure determination, in vitro anticancer activity on various human carcinoma cell lines and computational studies. J Photochem. Photobiol B 178:428-439
- Shu L, Cheung KL, Khor TO, Chen C, Kong AN (2010) Phytochemicals: cancer chemoprevention and suppression of tumor onset and metastasis. Cancer Metastasis Rev 29 (3):483-502
- Srivastava S, Somasagara RR, Hegde M, Nishana M, Tadi SK, Srivastava M, Choudhary B, Raghavan SC (2016) Quercetin, a Natural Flavonoid Interacts with DNA, Arrests Cell Cycle and Causes Tumor Regression by Activating Mitochondrial Pathway of Apoptosis Sci Rep (12)6:24049
- Sultana N (2011) Clinically useful anticancer, antitumor, and antiwrinkle agent, ursolic acid and related derivatives as medicinally important natural product. J Enzyme Inhib Med Chem 0:1-27
- Takada Y, Murakami A, Aggarwal BB (2005) Zerumbone abolishes NF-kappaB and IkappaBalpha kinase activation leading to suppression of antiapoptotic and metastatic gene expression, upregulation of apoptosis, and downregulation of invasion. Oncogene 24:6957–6969
- Tang DG and Porter AT (1997) Target to apoptosis: a hopeful weapon for prostate cancer. Prostate 32:284-93
- Toshiya K, Testuya T, Akira H, Takuji T (2012) Cancer chemoprevention through the induction of apoptosis by natural compounds. Biophys Chem 3(2):156-173
- Wang Z, Zhang Q, Zhou L, Liu G, Wu Q, Chen C (2020) Nor-wogonin flavone suppresses the growth of human colon cancer cells via mitochondrial mediated apoptosis, autophagy induction and triggering G2/M phase cell cycle arrest. J Buon 25(3):1449-1454
Toplam 39 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Biyokimya ve Hücre Biyolojisi (Diğer) |
| Bölüm | Research Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 26 Haziran 2023 |
| Kabul Tarihi | 7 Ocak 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 4 Sayı: 1 |
