Araştırma Makalesi
Yıl 2022,
Cilt: 28 Sayı: 1, 115 - 120, 25.02.2022
Öz
Vuralia turcica is an endemic plant species with a polycarpellary gynoecium and has the ability to survive in salt stress. However, the maintenance of this species is currently challenging because of climatic changes in the plant’s habitat, intensive agricultural purposes, grazing, and the like. Gynogenesis is a promising method for micropropagation and a useful technic for genetic engineering. The objective of this study was to analyze the gynogenesis of V. turcica. The experiments described here were implemented during the flowering time of V. turcica (spring) using unpollinated ovaries. Modified MS and B5 induction media and different plant growth regulators (BAP, GA3, 2,4-D, and KIN) were compared. The comparison of both media (MS and B5) showed that MS medium supplemented with 2,4-D and KIN was the best medium for in vitro unfertilized ovary culture and gave the best result of regenerated plants per 30 ovaries, and 92% callus was obtained. Only the control groups showed very low scores or no plants. From plant regeneration and subcultures, a total of 60 plants were produced, all green. These results are of interest to the in vitro conservation of endangered plant species like V. turcica.
Anahtar Kelimeler
Teşekkür
The author would like to thank Nezahat Gökyiğit Botanical Garden, Istanbul, Turkey, for providing research material and their support.
Kaynakça
- Bhojwani S S & Razdan M K (1996). Plant Tissue Culture : Theory and Practice, a Revised Edition. Elsevier.
- Cenkci S, Temel M, Kargioğlu M & Dayan S (2009). Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködüusing conventional and in vitro techniques. Turkish Journal of Biology 33: 327–33.
- Chand S. &Basu P (1998). Embryogenesis and plant regeneration from callus cultures derived from unpollinated ovaries of Hyoscyamus muticus L. Plant Cell Reports 17(4): 302-305.
- Chand S & Sahrawat A K (2007). Embryogenesis and plant regeneration from unpollinated ovary culture of Psoralea corylifolia. Biologia Plantarum 51(2): 223-228.
- Cunha A & Fernandes-Ferreira M (1999). Influence of medium parameters on somatic embryogenesis from hypocotyl explants of flax (Linum usitatissimum L.): effect of carbon source, total inorganic nitrogen and balance between ionic forms and interaction between calcium and zeatin. Journal of Plant Physiology 155: 591–597.
- Gamborg O L, Murashige T, Thorpe T A & Vasil I K (1976). Plant tissue culture media. In Vitro Cellular and Developmental Biology-Plant 12: 473–478.
- Hazarika R R & Chaturvedi R (2013). Establishment of dedifferentiated callus of haploid origin from unfertilized ovaries of tea (Camellia Sinensis (L.) O. Kuntze ) as a potential source of total phenolics and antioxidant activity. In Vitro Cellular and Developmental Biology - Plant 49(1): 60–69.
- Karadag B N, Yildirim E C & Tekdal D (2013). The comparison of plant regeneration between Jerusalem artichoke and purple potato cultured on MS media with different concentrations and combinations of plant growth regulators. International Journal of Bioscience, Biochemistry and Bioinformatics 3(2): 120-124.
- Keller J (1990). Culture of unpollinated ovules, ovaries, and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.). Euphytica 47(3): 241-247.
- Murashige T & Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497. Reiser L & Fischer R L (1993). The ovule and the embryo sac. Plant Cell 5: 1291-1301.
- Saad A I M & Elshahed A M (2012). Plant tissue culture media. In: Leva A & Rinaldi L (Eds.), Recent Advances in Plant in vitro Culture, Intech, UK, pp. 29-40.
- Sibi M L, Kobaissi A & Shekafandeh A (2001). Green haploid plants from unpollinated ovary culture in tetraploid wheat (Triticum durum Defs.). Euphytica 122(2): 351–59.
- Srivastava P, Singh M, Mathur P & Chaturverdi R (2009). In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica. Biologia Plantarum 53(2): 360-364.
- Tan K, Vural M & Küçüködük M (1983). An unusual new Thermopsis from Turkey. Notes Royal Botanical Garden Edinburgh 40(3): 515-518.
- Tekdal D & Cetiner S (2014a). In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica. Biologia 69: 863-869.
- Tekdal D & Cetiner S (2014b). In-ovule embryo culture of Thermopsis turcica. Journal of Animal and Plant Sciences 26(6): 1673-1679.
- Uysal T, Ertuğrul K, & Bozkurt M (2014). A new genus segregated from Thermopsis (Fabaceae: Papilionoideae): Vuralia. Plant Systematics and Evolution 300: 1627-1637.
- Van Tuyl J M, Van Diën M P, Van Creij M G M, Van Kleinwee T C M, Franken J & Bino R J (1991). Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Science 74(1): 115-126.
- Vural M (2009). Piyan (Thermopsis turcica). Bağbahçe 25: 14-16.
- Zhao L, Wu J, Diao Y & Hu Z (2012). Embryogenesis and plant regeneration from unpollinated ovaries of Amorphophallus konjac. African Journal of Biotechnology 11(70): 13472-13476.
Yıl 2022,
Cilt: 28 Sayı: 1, 115 - 120, 25.02.2022
Öz
Kaynakça
- Bhojwani S S & Razdan M K (1996). Plant Tissue Culture : Theory and Practice, a Revised Edition. Elsevier.
- Cenkci S, Temel M, Kargioğlu M & Dayan S (2009). Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködüusing conventional and in vitro techniques. Turkish Journal of Biology 33: 327–33.
- Chand S. &Basu P (1998). Embryogenesis and plant regeneration from callus cultures derived from unpollinated ovaries of Hyoscyamus muticus L. Plant Cell Reports 17(4): 302-305.
- Chand S & Sahrawat A K (2007). Embryogenesis and plant regeneration from unpollinated ovary culture of Psoralea corylifolia. Biologia Plantarum 51(2): 223-228.
- Cunha A & Fernandes-Ferreira M (1999). Influence of medium parameters on somatic embryogenesis from hypocotyl explants of flax (Linum usitatissimum L.): effect of carbon source, total inorganic nitrogen and balance between ionic forms and interaction between calcium and zeatin. Journal of Plant Physiology 155: 591–597.
- Gamborg O L, Murashige T, Thorpe T A & Vasil I K (1976). Plant tissue culture media. In Vitro Cellular and Developmental Biology-Plant 12: 473–478.
- Hazarika R R & Chaturvedi R (2013). Establishment of dedifferentiated callus of haploid origin from unfertilized ovaries of tea (Camellia Sinensis (L.) O. Kuntze ) as a potential source of total phenolics and antioxidant activity. In Vitro Cellular and Developmental Biology - Plant 49(1): 60–69.
- Karadag B N, Yildirim E C & Tekdal D (2013). The comparison of plant regeneration between Jerusalem artichoke and purple potato cultured on MS media with different concentrations and combinations of plant growth regulators. International Journal of Bioscience, Biochemistry and Bioinformatics 3(2): 120-124.
- Keller J (1990). Culture of unpollinated ovules, ovaries, and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.). Euphytica 47(3): 241-247.
- Murashige T & Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497. Reiser L & Fischer R L (1993). The ovule and the embryo sac. Plant Cell 5: 1291-1301.
- Saad A I M & Elshahed A M (2012). Plant tissue culture media. In: Leva A & Rinaldi L (Eds.), Recent Advances in Plant in vitro Culture, Intech, UK, pp. 29-40.
- Sibi M L, Kobaissi A & Shekafandeh A (2001). Green haploid plants from unpollinated ovary culture in tetraploid wheat (Triticum durum Defs.). Euphytica 122(2): 351–59.
- Srivastava P, Singh M, Mathur P & Chaturverdi R (2009). In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica. Biologia Plantarum 53(2): 360-364.
- Tan K, Vural M & Küçüködük M (1983). An unusual new Thermopsis from Turkey. Notes Royal Botanical Garden Edinburgh 40(3): 515-518.
- Tekdal D & Cetiner S (2014a). In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica. Biologia 69: 863-869.
- Tekdal D & Cetiner S (2014b). In-ovule embryo culture of Thermopsis turcica. Journal of Animal and Plant Sciences 26(6): 1673-1679.
- Uysal T, Ertuğrul K, & Bozkurt M (2014). A new genus segregated from Thermopsis (Fabaceae: Papilionoideae): Vuralia. Plant Systematics and Evolution 300: 1627-1637.
- Van Tuyl J M, Van Diën M P, Van Creij M G M, Van Kleinwee T C M, Franken J & Bino R J (1991). Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Science 74(1): 115-126.
- Vural M (2009). Piyan (Thermopsis turcica). Bağbahçe 25: 14-16.
- Zhao L, Wu J, Diao Y & Hu Z (2012). Embryogenesis and plant regeneration from unpollinated ovaries of Amorphophallus konjac. African Journal of Biotechnology 11(70): 13472-13476.
Toplam 20 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 25 Şubat 2022 |
| Gönderilme Tarihi | 10 Ağustos 2020 |
| Kabul Tarihi | 3 Ocak 2021 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 28 Sayı: 1 |
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