Research Article
İskenderun Körfezi’nden avlanan derin su pembe karidesi (Parapenaeus longirostris)’nin atıklarından elde edilen astaksantinin ekstraksiyonu ve karakterizasyonu
Abstract
Bu çalışmanın amacı, düşük değerli bir hammadde olan karides atıklarından yüksek değerli bir pigment olan astaksantinin izolasyonu için basit ve etkili bir yöntem önermektir. Bu çalışmada ekstraksiyon çözücüsü olarak aseton kullanılmıştır. TLC (Thin Layer Chromatography) için mobil faz olarak 3/7(v/v) oranında aseton/ heksan kullanılmıştır. Çalışmada kullanılan örnekler Parapenaeus longirostris türüne ait olup İskenderun Körfezi/ Türkiye’de faaliyette bulunan yerel balıkçılardan elde edilmiştir. Karides atıkları, buzla dolu steril bir kapta laboratuvara taşınmıştır. Karideslerin kullanılan atıkları sefalotoraks, karın kabuğu ve kuyruk kısmıdır. Sefalotoraks’a yapışan etler temizlenmiş, atıklar su ile yıkanmış ve örnekler 50 °C’ de etüvde kurutulmuştur. Polietilen torbalarla paketlenmiş ve kullanılıncaya kadar -18 °C’de saklanmıştır. Bu araştırmada astaksantin, organik çözücüler (petrol eteri ve aseton) kullanılarak karides kabuk atıklarından ekstrakte edilmiştir. Astaksantin pigmentinin karakterizasyonu, üç bant olarak serbest Astaksantin(Rf=0,43), Astaksantin monoester (Rf=0,56) ve Astaksantin diester (Rf=0,81) olarak tespit edilen Lorenz Todd standart kromatogramında belirtildiği Retardasyon faktörünü (Rf) karşılaştıran TLC ile gerçekleştirilmiştir.
Keywords
Thanks
Bu çalışma 23-27 Ağustos 2021 tarihinde Saraybosna/Bosna Hersek’te düzenlenen 5. ICABB(5th International Congress on Advances in Bioscience and Biotechnology) Kongresi’nde sözlü bildiri olarak sunulmuştur.
References
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- Referans 12 Paul, T., Halder, S. K., Das, A., Ghosh, K., Mandal, A., Payra, P., ... & Mondal, K. C. (2015). Production of chitin and bioactive materials from Black tiger shrimp (Penaeus monodon) shell waste by the treatment of bacterial protease cocktail. 3 Biotech, 5(4), 483-493.
- Referans 13 Senphan, T.,Benjakul,S.(2012). Compositions and yield of lipids extracted from hepatopancreas of Pacific White shrimp (Litopenaeus vannemei) as affected by prior autolysis, Food Chemistry, 134,829-835.
- Referans 14 Senphan,T., Benjakul,S.,& Kishimura, H.(2014).Characteristics and antioxidant activity of carotenoprotein from shells of Pacific white shrimp extracted using hepatopancrease proteases. Food Bioscience, 5,54-63.
- Referans 15 Sila, A., Ghlissi, Z., Kamoun, Z., Makni, M., Nasri, M., Bougatef, A., & Sahnoun, Z. (2015). Astaxanthin from shrimp by-products ameliorates nephropathy in diabetic rats. European journal of nutrition, 54(2), 301-307.
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- Referans 17 Ushakumari, U. N., & Ramanujan, R. (2012). Astaxanthin from shrimp shell waste. International journal of pharmaceutical chemistry research, 1(3), 1-6.
Extraction and characterization of astaxantin from the waste of deep water pink shrimp (Parapenaeus longirostris) obtained from İskenderun Bay
Abstract
The aim of this study is to purpose a simple and effective method for the isolation of high-value pigment astaxanthin from shrimp waste, a low-value raw material. In this study, acetone was used as an extraction solvent. Mobile phase for TLC was acetone:hexane in the ratio 3:7 (v/v). The samples used in the study belong to the Parapenaeus longirostris species and were obtained from local fishermen operating in the Iskenderun Bay / Turkey. Shrimp waste was transported to the laboratory in a sterile container filled with ice. The wastes used in shrimp are the cephalothorax, abdominal shell and tail portion. Adhering meat from the cephalothorax was removed and the waste was washed under water and samples were dried at 50 °C with drying oven. Packed in polyethylene bags and stored at -18 °C until use. In this study, astaxanthin was extracted from shrimp shell waste using organic solvents (petroleum ether and acetone). Characterization of Astaxanthin pigment was performed with TLC buy comparing the Retardation Factor (Rf) as indicated in the Lorenz Todd standard chromatogram, in which three bands of Astaxanthin (Rf=0.36), Astaxanthin monoester (Rf=0.60) and Astaxanthin diester (Rf=0.75) were detected.
Keywords
References
- Referans 1 Ambati, R. R., Phang, S. M., Ravi, S., & Aswathanarayana, R. G. (2014). Astaxanthin: sources, extraction, stability, biological activities and its commercial applications—a review. Marine drugs, 12(1), 128-152.
- Referans 2 Cahú, T.B., Santos,S.D.,Mendes,A.,Cordula,C.R., Chavante,S.F., Carvalho, L.B.,et. al (2012. Recovery of protein,chitin,carotenoids and glycosaminglycans from Pacific White shrimp (Litopenaeus vannamei) processing waste. Process Biochemistry, 47,570-577.
- Referans 3 Chesson, A., & Collins, A. (1997). Assessment of the role of diet in cancer prevention. Cancer letters, 114(1-2), 237-245.
- Referans 4 Choi, S., & Koo, S. (2005). Efficient syntheses of the keto-carotenoids canthaxanthin, astaxanthin, and astacene. The Journal of Organic Chemistry, 70(8), 3328-3331.
- Referans 5 Gómez-Estaca, J., Alemán, A., López-Caballero, M. E., Baccan, G. C., Montero, P., & Gómez-Guillén, M. C. (2019). Bioaccessibility and antimicrobial properties of a shrimp demineralization extract blended with chitosan as wrapping material in ready-to-eat raw salmon. Food chemistry, 276, 342-349.
- Referans 6 IMARC. (2020). Shrimp market:Global industry trends, share, size, growth, opportunity and forecast 2020-2025. USA: IMARC group.
- Referans 7 Kobayashi, M., & Sakamoto, Y. J. B. L. (1999). Singlet oxygen quenching ability of astaxanthin esters from the green alga Haematococcus pluvialis. Biotechnology Letters, 21(4), 265-269.
- Referans 8 Margalith, P. Z. (1999). Production of ketocarotenoids by microalgae. Applied microbiology and biotechnology, 51(4), 431-438.
- Referans 9 Marketstudyreport. (2019). Global shrimp market research report 2019. USA.
- Referans 10 Nair, S., Gagnon, J., Pelletier, C., Tchoukanova, N., Zhang, J., Ewart, H. S., ... & Wang, Y. (2017). Shrimp oil extracted from the shrimp processing waste reduces the development of insulin resistance and metabolic phenotypes in diet-induced obese rats. Applied Physiology, Nutrition, and Metabolism, 42(8), 841-849.
- Referans 11 Nirmal, N. P., Santivarangkna, C., Rajput, M. S., & Benjakul, S. (2020). Trends in shrimp processing waste utilization: An industrial prospective. Trends in Food Science & Technology.
- Referans 12 Paul, T., Halder, S. K., Das, A., Ghosh, K., Mandal, A., Payra, P., ... & Mondal, K. C. (2015). Production of chitin and bioactive materials from Black tiger shrimp (Penaeus monodon) shell waste by the treatment of bacterial protease cocktail. 3 Biotech, 5(4), 483-493.
- Referans 13 Senphan, T.,Benjakul,S.(2012). Compositions and yield of lipids extracted from hepatopancreas of Pacific White shrimp (Litopenaeus vannemei) as affected by prior autolysis, Food Chemistry, 134,829-835.
- Referans 14 Senphan,T., Benjakul,S.,& Kishimura, H.(2014).Characteristics and antioxidant activity of carotenoprotein from shells of Pacific white shrimp extracted using hepatopancrease proteases. Food Bioscience, 5,54-63.
- Referans 15 Sila, A., Ghlissi, Z., Kamoun, Z., Makni, M., Nasri, M., Bougatef, A., & Sahnoun, Z. (2015). Astaxanthin from shrimp by-products ameliorates nephropathy in diabetic rats. European journal of nutrition, 54(2), 301-307.
- Referans 16 Sindhu, S., & Sherief, P. M. (2011, August). Extraction, characterization, antioxidant and anti-inflammatory properties of carotenoids from the shell waste of arabian red shrimp Aristeus alcocki, ramadan 1938. In The open Conference proceedings journal (Vol. 2, No. 1).
- Referans 17 Ushakumari, U. N., & Ramanujan, R. (2012). Astaxanthin from shrimp shell waste. International journal of pharmaceutical chemistry research, 1(3), 1-6.
There are 17 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Food Engineering |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | August 31, 2022 |
| Submission Date | September 13, 2021 |
| Acceptance Date | July 30, 2022 |
| Published in Issue | Year 2022 Volume: 7 Issue: 2 |
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