Farelerde Sarı Kantaron (Hypericum perforatum) Yağının Yara İyileşme Üzerine Etkilerinin Araştırılması

Cansu Akdoğan; Yalçın Alper Özturan; Aykut Göktürk Üner; Yılmaz Kalkan; İbrahim Akın

Research Article

Farelerde Sarı Kantaron (Hypericum perforatum) Yağının Yara İyileşme Üzerine Etkilerinin Araştırılması

Year 2022, Volume: 1 Issue: 1, 1 - 7, 30.03.2022

Cansu Akdoğan Yalçın Alper Özturan Aykut Göktürk Üner Yılmaz Kalkan İbrahim Akın

Abstract

Amaç: Sarı kantaron otu (Hypericum perforatum) yağı geleneksel yara tedavisinde sıkça kullanılan bitkisel ürünlerden biri olsa da kontrollü hayvan deneyleri ile etkisinin daha ayrıntılı incelenmesi gerekmektedir. Çalışmada sarı kantaron yağının farelerde eksizyonel tam katmanlı deri yarası iyileşmesi üzerine etkilerinin araştırılması amaçlanmıştır. Materyal ve Metot: Bu amaçla 6 haftalık, erkek, 15 adet BALB/c ırkı fare kullanıldı. Fareler, her grupta 5’er adet fare olacak şekilde sarı kantaron yağı, zeytinyağı ve fizyolojik tuzlu su grubu olarak 3 gruba ayrıldı. Genel anestezi altında her farenin sırt derisine eksizyonel tam katmanlı 6 adet deri yarası açıldı. Yaraların iyileşme süreçlerinin takip ve kayıt edilebilmesi için yara bölgelerinden düzenli olarak fotoğraf alındı ve görüntü işleme teknikleri ile yara alanının günlük küçülmesi 3’er gün ara ile izlendi. İyileşen yara dokuları 22. günde ELISA yöntemi ile hidroksiprolin ve total protein düzeyleri ölçüldü. Bulgular: Elde edilen veriler ile yapılan istatistiksel çalışmada; gruplar arası yara iyileşme süresi, yara kapanma miktarı, hidroksiprolin ve total protein miktarları arasında istatistiksel olarak fark bulunmadı (p>0,05). Sonuç: Sonuçlar sarı kantaron otu yağının yara iyileşmesi üzerine olumsuz bir etkisinin olmadığını, ancak yara iyileşmesini hızlandırmadığına da işaret etmektedir.

Keywords

Fare, Sarı Kantaron Yağı, Hypericum perforatum, Tam Katmanlı Deri Yarası, Yara İyileşmesi

Supporting Institution

Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri tarafından desteklenmiştir .

Project Number

ADU BAP VTF-17062

Thanks

Yazarlar, sunulan çalışmaya yaptıkları değerli katkılar için, Murat Sarıerler, Nuh Kılıç, Nihal Yaşar Gül Satar ve Göksen Çeçen Ayalp’e teşekkür eder.

References

1. Karasu A., Bakır B.: Yara ve yara iyileşmesi. Veteriner Cerrahi Dergisi 2008, 14(1): 36-43.
2. Yanık K.: Veteriner Genel Cerrahi, Medipres Matbaacılık, Ankara, 2012, sayfa: 301-332.
3. Behm B., Babilas P., Landthaler M., Schreml S. Cytokines, chemokines and growth factors in wound healing. J. Eur. Acad. Dermatol. Venereol. 2012, (26): 812-820.
4. Nayak S. B., Sandiford S., Maxwell A.: Evaluation of the wound-healing activity of ethanolic extract of Morina citrifolia L. leaf. Evid. Based Complement. Alternat. Med. 2009, 6(3): 351-356.
5. Bekem A., Ünal A., Aydın H., Tüzün İ. S., Karşıdağ T.: Deri iyileşmesinin mekanik olarak incelenmesi. SDÜ-JESD. 2014, 2(3): 141-145.
6. Lindley L. E., Stojadinovic O., Partar I., Tomic-Canic M.: Biology and biomarkers for wound healing. Plast. Reconstr Surg. 2016, 138(3): 18-28.
7. Gurtner G. C., Werner S., Barrandon Y., Longaker M. T.: Wound repair and regeneration. Nature 2008, 453(7193): 314-21.
8. Balsa I. M., Culp W. T.: Wound care. Vet. Clin. North Am. Small Anim. Pract. 2015, 45(5): 1049-1065.
9. Honnegowda T. M., Kumar P., Udupa P., Rao P., Bhandary S., Mahato K. K., Sharan A., Mayya S. S.: Effect of limited access dressing on hydroxyproline and enzymatic antioxidant status in non healing chronic ulcers. Indian J. Plast. Surg. 2014, 47(2): 216-220.
10. Shukla A., Rasik A. M., Jain G. K., Shankar R., Kulshrestha D. K., Dhawan B. N.: In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J. Ethnopharmacol. 1999, 65(1): 1-11.
11. Nagelschmidt M., Struck H.: Hydroxyproline fractions in serum and urine of rats during wound healing. Res. Exp. Med. 1977, 170(3): 211-215.
12. Lin T. S., AbdLatiff A., Abd Hamid N. A., WaNgah W. Z., Mazlan M.: Evaluation of topical tocopherol cream on cutaneous wound healing in streptozotocin-induced diabetic rats. Evid. Based Complement. Alternat. Med. 2012, (2012): 491027.
13. Moreira C.F., Cassini-Vieira P., da Silva M. F., da Silva Barcelos L.: Skin wound healing model-excisional wounding and assessment of lesion area. J. Immunol. 2015, 22(5).
14. Bombardelli, E., Morazzoni P.: Hypericum perforatum. Fitoterapia 1995, (66): 43-68.
15. Bruneton, J.: Pharmacognosy, Phytochemistry and Medicinal Plants, Lavoisier Publishing, Paris, 1995, sayfa: 265.
16. Barnes J., Anderson L. A., Phillipson. J. D.: St. John's wort (Hypericum perforatum L.): A review of its chemistry, pharmacology and clinical properties. J. Pharm. Pharmacol. 2001, 53(5): 583-600.
17. Fu P., Zhang W. D., Liu R. H., Li T. Z., Shen Y. H., Li H. L., Chen H. S.: Two new xanthones from Hypericum japonicum. Nat. Prod. Res. 2006, 20(13): 1237-1240.
18. Mukherjee P. K., Verpoorte R., Suresh B.: Evaluation of in-vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J. Ethnopharmacol. 2000, 70(3): 315-321.
19. Hemmati A. A., Rashidi I., Jafari M.: Promotion of wound healing by Hypericum perforatum extract in rabbit. Jundishapur J. Nat. Pharm. Prod. 2007, 2(2): 78-86.
20. Greeson J. M., Sanford B., Monti D. A.: St. John’s wort (Hypericum perforatum): A review of the current pharmacological, toxicological, and clinical literature. Psychopharmacology. 2001, 153(4): 402-414.
21. Crockett S. L., Robson N. K.: Taxonomy and chemotaxonomy of the Genus Hypericum. Med. Aromat. Plant Sci. Biotechnol. 2011, 5(1): 1-13.
22. Buitrago S., Martin T. E., Tetens-Woodring J., Belicha-Villaneueva A., Wilding G. E.: Safety and efficacy of various combinations of injectable anesthetics in BALB/c mice. J AALAS 2008, (47): 11-17.
23. Liu X., Wang Z., Wang R., Zhao F., Shi P., Jiang Y., Pang X.: Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds. Int. J. Mol. Med. 2013, 31(2): 407-415.
24. Teoh S. L., Latiff A. A., Das S.: The effect of topical extract of Momordica charantia (bitter gourd) on wound healing in non diabetic rats and in rats with diabetes induced by streptozotocin. Clin. Exp. Dermatol. 2009, 34(7): 815-822.
25. Zomer H. D., Trentin A. G.: Skin wound healing in humans and mice: Challenges in translational research. J. Dermatol. Sci. 2018, 90(1): 3-12.
26. Gupta A., Kumar P.: Assessment of the histological state of the healing wound. Plast. Aesthet. Res. 2015, 2(5): 239-239.
27. Lucas Y., Niri R., Treuillet S., Douzi H., Castaneda B.: Wound Size Imaging: Ready for Smart Assessment and Monitoring. Adv. Wound Care. 2021, 10(11):641-661.
28. Kaplan B., Gönül B., Dinçer S., Dinçer Kaya F. N., Babül A.: Relationship between tensile strength, ascorbic acid, hydroxyproline, and zinc levels of rabbit full-thickness incision wound healing. Surg. Today. 2004, 34(9): 747-751.
29. Conte, M. S., Bradbury, A. W., Kolh, P., White, J. V., Dick, F., Fitridge, R., Mills J. L., Ricco B. J., Suresh K. R., Murad M. H., Diamant, M.: Global vascular guidelines on the management of chronic limb-threatening ischemia. J. Vasc. Surg. 2019, 69(6): 3-125.
30. Hepsağ F.: Geleneksel Yöntemlerle Osmaniye İlinde Üretilen Zeytinyağların Kalite Özelliklerinin Belirlenmesi. ADYÜTAYAM 2020, 8(1): 1-9.

Investigation of the Effects of St. John's Wort (Hypericum perforatum) Oil on Wound Healing in Mice

Year 2022, Volume: 1 Issue: 1, 1 - 7, 30.03.2022

Cansu Akdoğan Yalçın Alper Özturan Aykut Göktürk Üner Yılmaz Kalkan İbrahim Akın

Abstract

Objective: Although St.wort John's (Hypericum perforatum) oil is one of the most commonly used herbal products in traditional wound treatment, its effects should be examined further with controlled animal experiments. This study aimed to investigate the effects of St. John's wort oil on excisional full layer skin wound healing in mice. Material and Methods: For this aim, 6-week-old male, 15 BALB / c rats were used. The mice were divided into 3 groups as St. John's wort oil, olive oil, and physiological saline, each group of 5 mice. On the back skin of each mouse, six full-layered excisional skin wounds were created under anesthesia. The wound areas were photographed on a routine basis and the daily shrinkage of the wound area was observed with image processing techniques at three-day intervals in order to track and record the wound healing processes. On the 22nd day, wound tissues were collected and hydroxyproline and total protein levels were determined using an ELISA method. Results: The wound healing time, wound closure amount, hydroxyproline, and total protein levels between the groups were not statistically significant (p> 0.05). Conclusion: Based on the present study, St. John's wort oil does not have a negative effect on wound healing, also it does not speed up wound healing.

Keywords

Mice, St. John’s Worth, Hypericum perforatum, Full thickness skin wound, Wound

Project Number

ADU BAP VTF-17062

References

1. Karasu A., Bakır B.: Yara ve yara iyileşmesi. Veteriner Cerrahi Dergisi 2008, 14(1): 36-43.
2. Yanık K.: Veteriner Genel Cerrahi, Medipres Matbaacılık, Ankara, 2012, sayfa: 301-332.
3. Behm B., Babilas P., Landthaler M., Schreml S. Cytokines, chemokines and growth factors in wound healing. J. Eur. Acad. Dermatol. Venereol. 2012, (26): 812-820.
4. Nayak S. B., Sandiford S., Maxwell A.: Evaluation of the wound-healing activity of ethanolic extract of Morina citrifolia L. leaf. Evid. Based Complement. Alternat. Med. 2009, 6(3): 351-356.
5. Bekem A., Ünal A., Aydın H., Tüzün İ. S., Karşıdağ T.: Deri iyileşmesinin mekanik olarak incelenmesi. SDÜ-JESD. 2014, 2(3): 141-145.
6. Lindley L. E., Stojadinovic O., Partar I., Tomic-Canic M.: Biology and biomarkers for wound healing. Plast. Reconstr Surg. 2016, 138(3): 18-28.
7. Gurtner G. C., Werner S., Barrandon Y., Longaker M. T.: Wound repair and regeneration. Nature 2008, 453(7193): 314-21.
8. Balsa I. M., Culp W. T.: Wound care. Vet. Clin. North Am. Small Anim. Pract. 2015, 45(5): 1049-1065.
9. Honnegowda T. M., Kumar P., Udupa P., Rao P., Bhandary S., Mahato K. K., Sharan A., Mayya S. S.: Effect of limited access dressing on hydroxyproline and enzymatic antioxidant status in non healing chronic ulcers. Indian J. Plast. Surg. 2014, 47(2): 216-220.
10. Shukla A., Rasik A. M., Jain G. K., Shankar R., Kulshrestha D. K., Dhawan B. N.: In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J. Ethnopharmacol. 1999, 65(1): 1-11.
11. Nagelschmidt M., Struck H.: Hydroxyproline fractions in serum and urine of rats during wound healing. Res. Exp. Med. 1977, 170(3): 211-215.
12. Lin T. S., AbdLatiff A., Abd Hamid N. A., WaNgah W. Z., Mazlan M.: Evaluation of topical tocopherol cream on cutaneous wound healing in streptozotocin-induced diabetic rats. Evid. Based Complement. Alternat. Med. 2012, (2012): 491027.
13. Moreira C.F., Cassini-Vieira P., da Silva M. F., da Silva Barcelos L.: Skin wound healing model-excisional wounding and assessment of lesion area. J. Immunol. 2015, 22(5).
14. Bombardelli, E., Morazzoni P.: Hypericum perforatum. Fitoterapia 1995, (66): 43-68.
15. Bruneton, J.: Pharmacognosy, Phytochemistry and Medicinal Plants, Lavoisier Publishing, Paris, 1995, sayfa: 265.
16. Barnes J., Anderson L. A., Phillipson. J. D.: St. John's wort (Hypericum perforatum L.): A review of its chemistry, pharmacology and clinical properties. J. Pharm. Pharmacol. 2001, 53(5): 583-600.
17. Fu P., Zhang W. D., Liu R. H., Li T. Z., Shen Y. H., Li H. L., Chen H. S.: Two new xanthones from Hypericum japonicum. Nat. Prod. Res. 2006, 20(13): 1237-1240.
18. Mukherjee P. K., Verpoorte R., Suresh B.: Evaluation of in-vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J. Ethnopharmacol. 2000, 70(3): 315-321.
19. Hemmati A. A., Rashidi I., Jafari M.: Promotion of wound healing by Hypericum perforatum extract in rabbit. Jundishapur J. Nat. Pharm. Prod. 2007, 2(2): 78-86.
20. Greeson J. M., Sanford B., Monti D. A.: St. John’s wort (Hypericum perforatum): A review of the current pharmacological, toxicological, and clinical literature. Psychopharmacology. 2001, 153(4): 402-414.
21. Crockett S. L., Robson N. K.: Taxonomy and chemotaxonomy of the Genus Hypericum. Med. Aromat. Plant Sci. Biotechnol. 2011, 5(1): 1-13.
22. Buitrago S., Martin T. E., Tetens-Woodring J., Belicha-Villaneueva A., Wilding G. E.: Safety and efficacy of various combinations of injectable anesthetics in BALB/c mice. J AALAS 2008, (47): 11-17.
23. Liu X., Wang Z., Wang R., Zhao F., Shi P., Jiang Y., Pang X.: Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds. Int. J. Mol. Med. 2013, 31(2): 407-415.
24. Teoh S. L., Latiff A. A., Das S.: The effect of topical extract of Momordica charantia (bitter gourd) on wound healing in non diabetic rats and in rats with diabetes induced by streptozotocin. Clin. Exp. Dermatol. 2009, 34(7): 815-822.
25. Zomer H. D., Trentin A. G.: Skin wound healing in humans and mice: Challenges in translational research. J. Dermatol. Sci. 2018, 90(1): 3-12.
26. Gupta A., Kumar P.: Assessment of the histological state of the healing wound. Plast. Aesthet. Res. 2015, 2(5): 239-239.
27. Lucas Y., Niri R., Treuillet S., Douzi H., Castaneda B.: Wound Size Imaging: Ready for Smart Assessment and Monitoring. Adv. Wound Care. 2021, 10(11):641-661.
28. Kaplan B., Gönül B., Dinçer S., Dinçer Kaya F. N., Babül A.: Relationship between tensile strength, ascorbic acid, hydroxyproline, and zinc levels of rabbit full-thickness incision wound healing. Surg. Today. 2004, 34(9): 747-751.
29. Conte, M. S., Bradbury, A. W., Kolh, P., White, J. V., Dick, F., Fitridge, R., Mills J. L., Ricco B. J., Suresh K. R., Murad M. H., Diamant, M.: Global vascular guidelines on the management of chronic limb-threatening ischemia. J. Vasc. Surg. 2019, 69(6): 3-125.
30. Hepsağ F.: Geleneksel Yöntemlerle Osmaniye İlinde Üretilen Zeytinyağların Kalite Özelliklerinin Belirlenmesi. ADYÜTAYAM 2020, 8(1): 1-9.

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Details

Primary Language	Turkish
Subjects	Veterinary Surgery
Journal Section	Research articles
Authors	Cansu Akdoğan This is me 0000-0001-5442-5117 Yalçın Alper Özturan 0000-0003-0589-5686 Aykut Göktürk Üner This is me 0000-0002-9242-8279 Yılmaz Kalkan This is me 0000-0003-1261-5857 İbrahim Akın 0000-0002-4387-7115
Project Number	ADU BAP VTF-17062
Publication Date	March 30, 2022
Submission Date	May 18, 2021
Published in Issue	Year 2022 Volume: 1 Issue: 1

Cite

APA	Akdoğan, C., Özturan, Y. A., Üner, A. G., Kalkan, Y., et al. (2022). Farelerde Sarı Kantaron (Hypericum perforatum) Yağının Yara İyileşme Üzerine Etkilerinin Araştırılması. Türk Veteriner Cerrahi Dergisi, 1(1), 1-7.

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