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Karadeniz’de (Sinop Yarımadası Civarı) Avlanan İzmarit Balığı, Spicara smaris (Linnaeus, 1758), Etinin LC-MS/MS Kullanarak Amino Asit İçeriğinin Tespiti ve Ağırlık-Boy İlişkisi

Year 2019, Volume: 6 Issue: 2, 130 - 136, 22.04.2019
https://doi.org/10.30910/turkjans.556589

Abstract

Bu
çalışmada Karadeniz’de Sinop yarımadası civarında yakalanan izmarit balığı (Spicara smaris) etinin amino asit
içeriği ve bu balığın boy ağırlık ilişkisi belirlenmiştir. Amino asit
içeriğinin belirlenmesinde; bir karışımdaki istenen maddeyi ileri düzeyde
ayırıp tanımlayabilir ve ölçebilir özellikteki LC-MS / MS (Sıvı Kromatografi
Kütle Spektrometresi) cihazı kullanılmış ve bu yöntemle izmarit balıkları için
sonuçlar ilk kez rapor edilmiştir. Bu çalışmada esansiyel (EAA) ve esansiyel
olmayan (NEAA) toplam 19 adet amino asit tespit edilmiştir. Esansiyel amino
asitler toplam amino asit miktarının %52,44’ünü oluşturmuştur. İzmarit etinde
en yüksek oranda tespit edilen EAA miktarları sırasıyla lysin (2,10±0,215 g/100
g), leucine (1,68±0,115 g/100 g), arginine (1,18±0,030 g/100 g) ve valine (1,09±0,070
g/100 g) şeklindedir. İzmarit etinde en yüksek oranda tespit edilen NEAA
miktarları ise sırasıyla glutamic asit (2,88±0,010 g/100 g), aspartik asit
(2,16±0,280 g/100 g), alanine (1,32±0,000 g/100 g) ve glycine (1,05±0,060 g/100
g) şeklindedir. Araştırmada incelenen balıkların ağırlık - boy ilişkisi W =
0,0044TL3,3423, R2 = 0,990, N = 45, P < 0,05 şeklinde
hesaplanmıştır. Ağırlık boy - ilişkisi (WLR) denkleminin korelasyon katsayısı (r) istatistiksel olarak sıfırdan farklı
(P < 0,05), b değeri ise
istatistiksel olarak b = 3 den (izometrik büyüme) farklı bulunmuştur (Pauly t
test P < 0,05). Bu sonuçlar
Karadeniz’de uzatma ağlarıyla avlanan izmarit balıklarının AA bakımından zengin
ve pozitif allometrik büyüme özelliği gösterdiğini ortaya koymuştur.

References

  • Bilecenoğlu, M., Kaya, M., Cihangir, B., Çiçek, E. 2014. An updated checklist of the marine fishes of Turkey. Turkish Journal of Zoology, 38: 901-929.
  • Bilgin, Ö., Çarlı, U., Erdoğan, S., Mavış, M.E., Goksu-Gursu, G., Yılmaz, M. 2018. Karadeniz’de avlanan hamsi balığı, Engraulis encrasicolus, etinin amino asit içeriğinin LCMS/MS kullanılarak tespiti. Türk Tarım ve Doğa Bilimleri Dergisi, 5(4): 465-470.
  • Bilgin, S., Köse, Ö. 2018. Length-Weight Relationships (LWRs) of target fish turbot, Scophthalmus maximus(Pleuronectiformes: Scophthalmidae) and non-target fish thornback ray, Raja clavata (Rajiformes: Rajidae) caught by turbot gill net fishery in the Black Sea, Turkey. Cahiers de Biologie Marine, 59 : 615-622.
  • Bok, T.D., Gokturk, D., Kahraman, A.E., Alilci, T.Z. 2011. Length – weight relationships of 34 fish species from the Sea of Marmara, Turkey. Journal of Animal and Veterinary Advances, 10: 3037-3042.
  • Çiçek, E., Avşar, D. 2010. Growth and mortality of Spicara smaris (Linnaeus, 1758) off Karatas coast (Iskenderun Bay). Ecological Life Sciences, 5: 126-134.
  • Doğan, G., Ertan, Ö.O. 2017. Determination of amino acid and fatty acid composition of goldband goatfish [Upeneus moluccensis (Bleeker, 1855)] fishing from the Gulf of Antalya (Turkey). International Aquatic Research, 9: 313-327.
  • Erkan, N., Özden, Ö., Selçuk, A. 2010. Effect of frying, grilling, and streaming on amino acid composition of marine fishes. Journal of Medicinal Food, 13(6): 1524-1531.
  • Fidanbaş, Z.U.C., Bilgin, Ş., Ertan, Ö.O. 2015. Fatty acids - amino acid contents of some sea fish and importance in terms of nutrition. Eğirdir Su Ürünleri Fakültesi Dergisi, 11(2): 45-59.
  • Froese, R., Pauly, D. Editors. 2018. Fish Base. World Wide Web electronic publication. www.fishbase.org, version (10/2018).
  • Ismen, A. 1995. Growth, mortality and yield per recruit model of picarel (Spicara smaris L.) on the eastern Turkish Black Sea coast. Fisheries Research, 22: 299-308.
  • Kalaycı, F., Samsun, N., Bilgin, S., Samsun, O. 2007. Length-weight relationships of 10 fish species caught by bottom trawl and midwater trawl from the middle Black Sea, Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 7: 33-36.
  • Mazlum, R.E., Turan, D., Bilgin, S. 2015. Length-weight relationships of nine Cyprinid species from inland waters of Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 15: 381-384.
  • Özden, Ö., Erkan, N. 2011. A preliminary study of amino acid and mineral profiles of important and estimable 21 seafood species. British Food Journal, 113(4): 457-469.
  • Pauly, D. 1984. Fish population dynamics in tropical water: a manual for use with programmable calculators. ICLARM Studies and Reviews 1: 1-8.
  • Snedecor, G.W., Cochran, W.G. 1989. Statistical methods, eighth edition, Iowa State University Press, Ames, Iowa, 803 pp.
  • TÜİK, 2017. Türkiye İstatistik Kurumu, Su Ürünleri İstatistikleri. Ankara.
  • Yeldan, H., Avşar, D., Özütok, M., Çiçek, E. 2003. Growth and reproduction peculiarities of the picarel (Spicare smaris L., 1758) from the Babadıllimanı Bigth (Silifke-İçel). E.Ü. Journal of fisheries and Aquatic Sciences, 20: 35-42.

Determination of Amino Acid Content of Picarel (Spicara smaris) Caught in the Black Sea (Around Sinop Peninsula) Using LC-MS / MS and Its Weight-Length Relationship

Year 2019, Volume: 6 Issue: 2, 130 - 136, 22.04.2019
https://doi.org/10.30910/turkjans.556589

Abstract

In this study, the amino acid content and the weight - length
relationship (WLR) of the picarel (Spicara
smaris
) caught off Sinop peninsula in the Black Sea were determined.
Determination of amino acid content of picarel; the LC-MS / MS (Liquid
Chromatography Mass Spectrometry) devices was used and the results obtained
from this method were reported for the first time for picarel. A total of 19
amino acids were identified as essential (EAA) and non-essential amino acids (NEAA).
The EAAs composed for 52.44% of the total values of amino acids. The highest
amounts of EAA detected in the meat of picarel were lysine (2.10 ± 0.215 g / 100 g), leucine (1.68 ± 0.115
g / 100 g),
arginine (1.18 ± 0.030 g and valine (1.09 ± 0.070 g / 100 g), respectively. The
highest amounts of NEAA in the meat of picarel were determined as glutamic acid
(2.88 ± 0.010 g / 100 g),
aspartic acid (2.16 ± 0.280 g / 100
g), alanine (1.32 ± 0.000 g / 100 g ) and glycine (1.05 ±
0.060 g / 100 g),
respectively. The weight – length relationship (WLR) of the fish was calculated
as W = 0.0044TL3.3423, R2 = 0.990, N = 45, P < 0.05.
The correlation coefficient (r) of
the WLR equation was statistically different from zero (P < 0.05) and also b value was statistically different from
b = 3 (isometric growth) (Pauly t
test P < 0.05). These results show that the picarel caught in the Black Sea
have rich AA contents and show positive allometric growth.

References

  • Bilecenoğlu, M., Kaya, M., Cihangir, B., Çiçek, E. 2014. An updated checklist of the marine fishes of Turkey. Turkish Journal of Zoology, 38: 901-929.
  • Bilgin, Ö., Çarlı, U., Erdoğan, S., Mavış, M.E., Goksu-Gursu, G., Yılmaz, M. 2018. Karadeniz’de avlanan hamsi balığı, Engraulis encrasicolus, etinin amino asit içeriğinin LCMS/MS kullanılarak tespiti. Türk Tarım ve Doğa Bilimleri Dergisi, 5(4): 465-470.
  • Bilgin, S., Köse, Ö. 2018. Length-Weight Relationships (LWRs) of target fish turbot, Scophthalmus maximus(Pleuronectiformes: Scophthalmidae) and non-target fish thornback ray, Raja clavata (Rajiformes: Rajidae) caught by turbot gill net fishery in the Black Sea, Turkey. Cahiers de Biologie Marine, 59 : 615-622.
  • Bok, T.D., Gokturk, D., Kahraman, A.E., Alilci, T.Z. 2011. Length – weight relationships of 34 fish species from the Sea of Marmara, Turkey. Journal of Animal and Veterinary Advances, 10: 3037-3042.
  • Çiçek, E., Avşar, D. 2010. Growth and mortality of Spicara smaris (Linnaeus, 1758) off Karatas coast (Iskenderun Bay). Ecological Life Sciences, 5: 126-134.
  • Doğan, G., Ertan, Ö.O. 2017. Determination of amino acid and fatty acid composition of goldband goatfish [Upeneus moluccensis (Bleeker, 1855)] fishing from the Gulf of Antalya (Turkey). International Aquatic Research, 9: 313-327.
  • Erkan, N., Özden, Ö., Selçuk, A. 2010. Effect of frying, grilling, and streaming on amino acid composition of marine fishes. Journal of Medicinal Food, 13(6): 1524-1531.
  • Fidanbaş, Z.U.C., Bilgin, Ş., Ertan, Ö.O. 2015. Fatty acids - amino acid contents of some sea fish and importance in terms of nutrition. Eğirdir Su Ürünleri Fakültesi Dergisi, 11(2): 45-59.
  • Froese, R., Pauly, D. Editors. 2018. Fish Base. World Wide Web electronic publication. www.fishbase.org, version (10/2018).
  • Ismen, A. 1995. Growth, mortality and yield per recruit model of picarel (Spicara smaris L.) on the eastern Turkish Black Sea coast. Fisheries Research, 22: 299-308.
  • Kalaycı, F., Samsun, N., Bilgin, S., Samsun, O. 2007. Length-weight relationships of 10 fish species caught by bottom trawl and midwater trawl from the middle Black Sea, Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 7: 33-36.
  • Mazlum, R.E., Turan, D., Bilgin, S. 2015. Length-weight relationships of nine Cyprinid species from inland waters of Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 15: 381-384.
  • Özden, Ö., Erkan, N. 2011. A preliminary study of amino acid and mineral profiles of important and estimable 21 seafood species. British Food Journal, 113(4): 457-469.
  • Pauly, D. 1984. Fish population dynamics in tropical water: a manual for use with programmable calculators. ICLARM Studies and Reviews 1: 1-8.
  • Snedecor, G.W., Cochran, W.G. 1989. Statistical methods, eighth edition, Iowa State University Press, Ames, Iowa, 803 pp.
  • TÜİK, 2017. Türkiye İstatistik Kurumu, Su Ürünleri İstatistikleri. Ankara.
  • Yeldan, H., Avşar, D., Özütok, M., Çiçek, E. 2003. Growth and reproduction peculiarities of the picarel (Spicare smaris L., 1758) from the Babadıllimanı Bigth (Silifke-İçel). E.Ü. Journal of fisheries and Aquatic Sciences, 20: 35-42.
There are 17 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Özlem Bilgin

Uğur Çarlı This is me

Selahattin Erdoğan This is me

Murat Emrah Maviş This is me

Gökçe Göksu Gürsu This is me

Muhittin Yılmaz This is me

Publication Date April 22, 2019
Submission Date May 17, 2018
Published in Issue Year 2019 Volume: 6 Issue: 2

Cite

APA Bilgin, Ö., Çarlı, U., Erdoğan, S., Maviş, M. E., et al. (2019). Karadeniz’de (Sinop Yarımadası Civarı) Avlanan İzmarit Balığı, Spicara smaris (Linnaeus, 1758), Etinin LC-MS/MS Kullanarak Amino Asit İçeriğinin Tespiti ve Ağırlık-Boy İlişkisi. Türk Tarım Ve Doğa Bilimleri Dergisi, 6(2), 130-136. https://doi.org/10.30910/turkjans.556589