Technical Brief
BibTex RIS Cite
Year 2023, Volume: 41 Issue: 1, 202 - 208, 14.03.2023

Abstract

References

  • REFERENCES
  • [1] Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007;70:461–477. [Crossref]
  • [2] Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul-Enein HY. Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Anal Lett 2004;37:1859–1869. [Crossref]
  • [3] Demirtas I, Erenler R, Elmastas M, Goktasoglu A. Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble frac-tion. Food Chem 2013;136:34–40. [Crossref]
  • [4] Sahin Yaglioglu A, Akdulum B, Erenler R, Demirtas I, Telci I, Tekin S. Antiproliferative activity of pen-tadeca-(8E, 13Z) dien-11-yn-2-one and (E)-1,8-pentadecadiene from Echinacea pallida (Nutt.) Nutt. Roots. Med Chem Res 2013;22:2946–2953. [Crossref]
  • [5] Baser KHC. Aromatic biodiversity among the flowering plant taxa of Turkey. Pure Appl Chem 2002;74:527–545. [Crossref]
  • [6] Bouyahya A, Chamkhi I, Benali T, Guaouguaou FE, Balahbib A, El Omari N, et al. Traditional use, phytochemistry, toxicology, and pharmacol-ogy of Origanum majorana L. J Ethnopharmacol 2021;265:113318. [Crossref]
  • [7] Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, et al. Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. J Sci Food Agric 2016;96:822–836. [Crossref]
  • [8] Erenler R, Adak T, Karan T, Elmastas M, Yildiz I, Aksit H, et al. Chemical Constituents isolated from Origanum solymicum with Antioxidant activi-ties. ICONTES2017: International Conference on Technology, Engineering and Science; 2017 Oct 26- 29; Antalya, Turkey: ISRES Publishing; 2017. pp. 139–145.
  • [9] Erenler R, Meral B, Sen O, Elmastas M, Aydin A, Eminagaoglu O, et al. Bioassay-guided isola-tion, identification of compounds from Origanum rotundifolium and investigation of their antipro-liferative and antioxidant activities. Pharm Biol 2017;55:1646–1653. [Crossref]
  • [10] Elmastas M, Celik SM, Genc N, Aksit H, Erenler R, Gulcin I. Antioxidant activity of an anatolian herbal tea—Origanum minutiflorum: isolation and char-acterization of its secondary metabolites. Int J Food Prop 2018;21:374–384. [Crossref]
  • [11] Pezzani R, Vitalini S, Iriti M. Bioactivities of Origanum vulgare L.: An update. Phytochem Rev 2017;16: 1253–1268. [Crossref]
  • [12] Leyva-López N, Gutiérrez-Grijalva EP, Vazquez-Olivo G, Heredia JB. Essential oils of oregano: Biological activity beyond their antimicrobial prop-erties. Molecules 2017;22:989. [Crossref]
  • [13] Bayir B, Gunduz H, Usta T, Sahin E, Ozdemir Z, Kayır O, et al. Chemical composition of essential oil from Marrubium Vulgare L. leaves. J New Results Sci 2014;6:44–50.
  • [14] Kaya G, Karakaya R, Tilgel E, Sandikci M, Yucel E, Cicek G, et al. Essential oil constituents of Thuja ori-entalis berries. J New Results Sci 2014;7:1–6.
  • [15] Türkmen N, Oz A, Sonmez A, Erol T, Gulumser D, Yurdakul B, et al. Chemical composition of essen-tial oil from Rosmarinus Officinalis L. leaves. J New Results Sci 2014;6:27–31.
  • [16] Kosakowska O, Czupa W. Morphological and chem-ical variability of common oregano (Origanum vul-gare L. subsp. vulgare) occurring in eastern Poland. Herba Pol 2018;64:11–21. [Crossref]
  • [17] Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some essential oils—present status and future perspectives. Medicines 2017;4:58. [Crossref]
  • [18] Lombrea A, Antal D, Ardelean F, Avram S, Pavel IZ, Vlaia L, et al. A recent insight regarding the phytochemistry and bioactivity of Origanum vul- gare L. essential oil. Int J Mol Sci 2020;21:9653. [Crossref]
  • [19] Nanni V, Di Marco G, Sacchetti G, Canini A, Gismondi A. Oregano phytocomplex induces pro-grammed cell death in melanoma lines via mito-chondria and DNA damage. Foods 2020;9:1486.[Crossref]
  • [20] Andrew BM, Rex GC, Kim O, Juan AFS, Luis VE, Ballantines FA, et al. Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines. J Med Plants Res 2018;12:42–49. [Crossref]
  • [21] Koleva V, Dragoeva A, Stoyanova Z, Yordanova Z, Ali S, Uzunov NM, et al. In vitro cytotoxicity of alle-lopathic plants Adonis vernalis L. Origanum vulgare ssp. vulgare L. and Nepeta nuda subsp. nuda. Acta Sci Nat 2018;5:64–69. [Crossref]
  • [22] Grbović F, Stanković MS, Ćurčić M, Đorđević N, Šeklić D, Topuzović M, et al. In vitro cytotoxic activity of Origanum vulgare L. on HCT-116 and MDA-MB-231 cell lines. Plants 2013;2:371–378.[Crossref]
  • [23] Nile SH, Nile AS, Keum YS. Total phenolics, antioxi-dant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods. 3 Biotech 2017;7:76. [Crossref]
  • [24] Kokkini S, Karousou R, Dardioti A, Krigas N, Lanaras T. Autumn essential oils of Greek oregano. Phytochemistry 1997;44:883–886. [Crossref]
  • [25] Al-Kalaldeh JZ, Abu-Dahab R, Afifi FU. Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells. Nutr Res 2010;30:271–278. [Crossref]
  • [26] Begnini KR, Nedel F, Lund RG, Carvalho PHA, Rodrigues MRA, Beira FTA, et al. Composition and antiproliferative effect of essential oil of Origanum vulgare against tumor cell lines. J Med Food 2014;17:1129–1133. [Crossref]
  • [27] Balusamy SR, Perumalsamy H, Huq MA, Balasubramanian B. Anti-proliferative activity of Origanum vulgare inhibited lipogenesis and induced mitochondrial mediated apoptosis in human stomach cancer cell lines. Biomed Pharmacother 2018;108:1835–1844. [Crossref]
  • [28] Karan T, Simsek S, Yildiz I, Erenler R. Chemical composition and insecticidal activity of Origanum syriacum L. essential oil against Sitophilus ory-zae and Rhyzopertha dominica. Int J Sec Metabol 2018;5: 87–93. [Crossref]
  • [29] Karan T, Yildiz I, Aydin A, Erenler R. Inhibition of various cancer cells proliferation of bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Rec Nat Prod 2018;12:273–283. [Crossref]
  • [30] Erenler R, Demirtas I, Karan T, Gul F, Kayir O, Karakoc OC. Chemical constituents, quantitative analysis and insecticidal activities of plant extract and essential oil from Origanum onites L. Trends Phytochem Res 2018;2:91–96.
  • [31] Okten S, Cakmak O, Erenler R, Yuce O, Tekin S. Simple and convenient preparation of novel 6,8–disubstituted quinoline derivatives and their promising anticancer activities. Turk J Chem 2013;37:896–908. [Crossref]
  • [32] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55– 63. [Crossref]
  • [33] Decker T, Lohmann-Matthes ML. A quick and simple method for the quantitation of lactate dehy-drogenase release in measurements of cellular cyto-toxicity and tumor necrosis factor (TNF) activity. J Immunol Methods 1988;115:61–69. [Crossref]
  • [34] Adams RP. Identification of essential oil components by Gas chromatography/Mass spectrometry. 4th ed. Illinois, USA: Allured Publishing Corporation; 2007.
  • [35] Babushok V, Linstrom P, Zenkevich I. Retention indices for frequently reported compounds of plant essential oils. J Phys Chem Ref Data 2011;40:043101.[Crossref]
  • [36] Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food Chem Toxicol 2008;46:446–475. [Crossref]
  • [37] Xie Y, Huang Q, Rao Y, Hong L, Zhang D. Efficacy of Origanum vulgare essential oil and carvacrol against the housefly, Musca domestica L.(Diptera: Muscidae). Environ Sci Pollut Res Int 2019;26:23824–23831. [Crossref]
  • [38] Javed H, Meeran MFN, Jha NK, Ojha S. Carvacrol, a plant metabolite targeting viral protease (Mpro) and ACE2 in host cells can be a possible candidate for COVID-19. Front Plant Sci 2021;11:1–10. [Crossref]

Antiproliferative activity and cytotoxic effect of essential oil and water extract from origanum Vulgare L.

Year 2023, Volume: 41 Issue: 1, 202 - 208, 14.03.2023

Abstract

Origanum vulgare L., an aromatic and medicinal plant has been used extensively for the food and pharmaceutical industry. In this work, the essential oil was generated by steam distillation and the compounds were identified by GC-MS analysis. Carvacrol was found as a major product. O. vulgare was extracted with water (60 °C) to yield the water extract as well. The antiproliferative activity and cytotoxic effect of essential oi l an d ex tr act we re investigated using MTT [3- (4,5-dimethyl-thiazol-2-yl) -2,5-diphenyl tetrazolium bromide] and LDH (Lactate dehydrogenase) techniques, respectively. A549 (human lung carcinoma), Hep3B (hepatocellular carcinoma), HT29 (human colon carcinoma), MCF7 (human breast adenocarcinoma) cancerous cell lines, FL (human amnion cells) normal cell lines were used for essential oil and extract, while cisplatin and 5-FU were exploited as standards. The essential oil revealed the significant activity against A549 (IC50, 27.2 µg/mL), Hep3B (IC50, 7.4 µg/mL), and MCF-7 (IC50, 7.1 µg/mL) cancerous cell lines as compared to standards. Moreover, extract displayed outstanding activity against Hep3B (IC50, 27.2 µg/mL) and MCF-7 (IC50, 10.8 µg/mL) cell lines. The activity of essential oil may be due to carvacrol since carvacrol is the main constituent of essential oil with a high percentage (90.4%) or due to the synergistic effect of the compounds in the essential oil.

References

  • REFERENCES
  • [1] Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007;70:461–477. [Crossref]
  • [2] Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul-Enein HY. Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Anal Lett 2004;37:1859–1869. [Crossref]
  • [3] Demirtas I, Erenler R, Elmastas M, Goktasoglu A. Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble frac-tion. Food Chem 2013;136:34–40. [Crossref]
  • [4] Sahin Yaglioglu A, Akdulum B, Erenler R, Demirtas I, Telci I, Tekin S. Antiproliferative activity of pen-tadeca-(8E, 13Z) dien-11-yn-2-one and (E)-1,8-pentadecadiene from Echinacea pallida (Nutt.) Nutt. Roots. Med Chem Res 2013;22:2946–2953. [Crossref]
  • [5] Baser KHC. Aromatic biodiversity among the flowering plant taxa of Turkey. Pure Appl Chem 2002;74:527–545. [Crossref]
  • [6] Bouyahya A, Chamkhi I, Benali T, Guaouguaou FE, Balahbib A, El Omari N, et al. Traditional use, phytochemistry, toxicology, and pharmacol-ogy of Origanum majorana L. J Ethnopharmacol 2021;265:113318. [Crossref]
  • [7] Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, et al. Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. J Sci Food Agric 2016;96:822–836. [Crossref]
  • [8] Erenler R, Adak T, Karan T, Elmastas M, Yildiz I, Aksit H, et al. Chemical Constituents isolated from Origanum solymicum with Antioxidant activi-ties. ICONTES2017: International Conference on Technology, Engineering and Science; 2017 Oct 26- 29; Antalya, Turkey: ISRES Publishing; 2017. pp. 139–145.
  • [9] Erenler R, Meral B, Sen O, Elmastas M, Aydin A, Eminagaoglu O, et al. Bioassay-guided isola-tion, identification of compounds from Origanum rotundifolium and investigation of their antipro-liferative and antioxidant activities. Pharm Biol 2017;55:1646–1653. [Crossref]
  • [10] Elmastas M, Celik SM, Genc N, Aksit H, Erenler R, Gulcin I. Antioxidant activity of an anatolian herbal tea—Origanum minutiflorum: isolation and char-acterization of its secondary metabolites. Int J Food Prop 2018;21:374–384. [Crossref]
  • [11] Pezzani R, Vitalini S, Iriti M. Bioactivities of Origanum vulgare L.: An update. Phytochem Rev 2017;16: 1253–1268. [Crossref]
  • [12] Leyva-López N, Gutiérrez-Grijalva EP, Vazquez-Olivo G, Heredia JB. Essential oils of oregano: Biological activity beyond their antimicrobial prop-erties. Molecules 2017;22:989. [Crossref]
  • [13] Bayir B, Gunduz H, Usta T, Sahin E, Ozdemir Z, Kayır O, et al. Chemical composition of essential oil from Marrubium Vulgare L. leaves. J New Results Sci 2014;6:44–50.
  • [14] Kaya G, Karakaya R, Tilgel E, Sandikci M, Yucel E, Cicek G, et al. Essential oil constituents of Thuja ori-entalis berries. J New Results Sci 2014;7:1–6.
  • [15] Türkmen N, Oz A, Sonmez A, Erol T, Gulumser D, Yurdakul B, et al. Chemical composition of essen-tial oil from Rosmarinus Officinalis L. leaves. J New Results Sci 2014;6:27–31.
  • [16] Kosakowska O, Czupa W. Morphological and chem-ical variability of common oregano (Origanum vul-gare L. subsp. vulgare) occurring in eastern Poland. Herba Pol 2018;64:11–21. [Crossref]
  • [17] Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some essential oils—present status and future perspectives. Medicines 2017;4:58. [Crossref]
  • [18] Lombrea A, Antal D, Ardelean F, Avram S, Pavel IZ, Vlaia L, et al. A recent insight regarding the phytochemistry and bioactivity of Origanum vul- gare L. essential oil. Int J Mol Sci 2020;21:9653. [Crossref]
  • [19] Nanni V, Di Marco G, Sacchetti G, Canini A, Gismondi A. Oregano phytocomplex induces pro-grammed cell death in melanoma lines via mito-chondria and DNA damage. Foods 2020;9:1486.[Crossref]
  • [20] Andrew BM, Rex GC, Kim O, Juan AFS, Luis VE, Ballantines FA, et al. Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines. J Med Plants Res 2018;12:42–49. [Crossref]
  • [21] Koleva V, Dragoeva A, Stoyanova Z, Yordanova Z, Ali S, Uzunov NM, et al. In vitro cytotoxicity of alle-lopathic plants Adonis vernalis L. Origanum vulgare ssp. vulgare L. and Nepeta nuda subsp. nuda. Acta Sci Nat 2018;5:64–69. [Crossref]
  • [22] Grbović F, Stanković MS, Ćurčić M, Đorđević N, Šeklić D, Topuzović M, et al. In vitro cytotoxic activity of Origanum vulgare L. on HCT-116 and MDA-MB-231 cell lines. Plants 2013;2:371–378.[Crossref]
  • [23] Nile SH, Nile AS, Keum YS. Total phenolics, antioxi-dant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods. 3 Biotech 2017;7:76. [Crossref]
  • [24] Kokkini S, Karousou R, Dardioti A, Krigas N, Lanaras T. Autumn essential oils of Greek oregano. Phytochemistry 1997;44:883–886. [Crossref]
  • [25] Al-Kalaldeh JZ, Abu-Dahab R, Afifi FU. Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells. Nutr Res 2010;30:271–278. [Crossref]
  • [26] Begnini KR, Nedel F, Lund RG, Carvalho PHA, Rodrigues MRA, Beira FTA, et al. Composition and antiproliferative effect of essential oil of Origanum vulgare against tumor cell lines. J Med Food 2014;17:1129–1133. [Crossref]
  • [27] Balusamy SR, Perumalsamy H, Huq MA, Balasubramanian B. Anti-proliferative activity of Origanum vulgare inhibited lipogenesis and induced mitochondrial mediated apoptosis in human stomach cancer cell lines. Biomed Pharmacother 2018;108:1835–1844. [Crossref]
  • [28] Karan T, Simsek S, Yildiz I, Erenler R. Chemical composition and insecticidal activity of Origanum syriacum L. essential oil against Sitophilus ory-zae and Rhyzopertha dominica. Int J Sec Metabol 2018;5: 87–93. [Crossref]
  • [29] Karan T, Yildiz I, Aydin A, Erenler R. Inhibition of various cancer cells proliferation of bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Rec Nat Prod 2018;12:273–283. [Crossref]
  • [30] Erenler R, Demirtas I, Karan T, Gul F, Kayir O, Karakoc OC. Chemical constituents, quantitative analysis and insecticidal activities of plant extract and essential oil from Origanum onites L. Trends Phytochem Res 2018;2:91–96.
  • [31] Okten S, Cakmak O, Erenler R, Yuce O, Tekin S. Simple and convenient preparation of novel 6,8–disubstituted quinoline derivatives and their promising anticancer activities. Turk J Chem 2013;37:896–908. [Crossref]
  • [32] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55– 63. [Crossref]
  • [33] Decker T, Lohmann-Matthes ML. A quick and simple method for the quantitation of lactate dehy-drogenase release in measurements of cellular cyto-toxicity and tumor necrosis factor (TNF) activity. J Immunol Methods 1988;115:61–69. [Crossref]
  • [34] Adams RP. Identification of essential oil components by Gas chromatography/Mass spectrometry. 4th ed. Illinois, USA: Allured Publishing Corporation; 2007.
  • [35] Babushok V, Linstrom P, Zenkevich I. Retention indices for frequently reported compounds of plant essential oils. J Phys Chem Ref Data 2011;40:043101.[Crossref]
  • [36] Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food Chem Toxicol 2008;46:446–475. [Crossref]
  • [37] Xie Y, Huang Q, Rao Y, Hong L, Zhang D. Efficacy of Origanum vulgare essential oil and carvacrol against the housefly, Musca domestica L.(Diptera: Muscidae). Environ Sci Pollut Res Int 2019;26:23824–23831. [Crossref]
  • [38] Javed H, Meeran MFN, Jha NK, Ojha S. Carvacrol, a plant metabolite targeting viral protease (Mpro) and ACE2 in host cells can be a possible candidate for COVID-19. Front Plant Sci 2021;11:1–10. [Crossref]
There are 39 citations in total.

Details

Primary Language English
Subjects Empirical Software Engineering
Journal Section Technical Note
Authors

Ramazan Erenler 0000-0002-0505-3190

Ümran Ece Çarlık This is me 0000-0003-1902-6959

Ali Aydın This is me 0000-0002-9550-9111

Publication Date March 14, 2023
Submission Date June 19, 2021
Published in Issue Year 2023 Volume: 41 Issue: 1

Cite

Vancouver Erenler R, Çarlık ÜE, Aydın A. Antiproliferative activity and cytotoxic effect of essential oil and water extract from origanum Vulgare L. SIGMA. 2023;41(1):202-8.

IMPORTANT NOTE: JOURNAL SUBMISSION LINK https://eds.yildiz.edu.tr/sigma/