Research Article
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Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey

Year 2023, Volume: 27 Issue: 4, 920 - 929, 25.08.2023

Mehtap Usta

https://doi.org/10.16984/saufenbilder.1284027

Abstract

Honey is a functional food with high nutritional properties and rich in bioactive components. The fact that the biological activity of honey differs according to botanical origin, geography and climatic characteristics necessitates a comprehensive consideration of monofloral and multifloral honeys produced in Turkey. The adoption of the understanding of replacing natural preservatives with synthetic products enables the search for alternative uses of honey. For this purpose, the antimicrobial activities of linden, rhododendron, chestnut and multifloral honeys were determined in this study and their antimicrobial activities were compared. Obtained zone diameters were statistically compared with the IBM SPSS version 22.0 statistical program. According to the results, it was determined that in general, multifloral honey has higher antimicrobial activity than monofloral honey, rhododendron honey from monofloral honeys shows strong inhibition against the tested microorganisms, and Yalova linden honey has the weakest antibacterial effect. It was determined that the antimicrobial activity in all honey varieties was generally bacteria > yeast > mold respectively.

Keywords

Antimicrobial activity honey microbiology monofloral multifloral

References

  • S. Bogdanov, K. Ruoff, L. Persano Oddo. “Physico-chemical methods for the characterisation of unifloral honeys: A review,” Apidologie, vol. 35, no. 1, pp. 4-17, 2004.
  • E. Mahmoodi-Khaledi, J. Lozano-Sánchez, A. Bakhouche, M. Habibi-Rezaei, I. Sadeghian, A. Segura-Carretero. “Physicochemical properties and biological activities of honeys from different geographical and botanical origins in Iran,” European Food Research and Technology, vol. 243, no. 6, pp. 1019-1030, 2017.
  • S. Samarghandian, T. Farkhondeh, F. Samini. “Honey and Health: A Review of Recent Clinical Research,” Pharmacognosy Research, Vol. 9, no. 2, pp. 121-127, 2017.
  • F. J. Leyva-Jimenez, J. Lozano-Sanchez, I. BorrasLinares, M. L. Cadiz-Gurrea, E. MahmoodiKhaledi. “Potential antimicrobial activity of honey phenolic compounds against Gram positive and Gram negative bacteria,” LWT- Food Science and Technology, vol. 101, pp. 236-245, 2018.
  • E. I. Ramsay, S. Rao, L. Madathil, S. K. Hegde, M. P. Baliga-Rao, T. George, M. S. Baliga. “Honey in oral health and care: A mini review,” Journal of Oral Biosciences, vol. 61, pp. 32- 36, 2019.
  • N. Gheldof, X. H. Wang, N. J. Engeseth. “Buckwheat honey increases serum antioxidant capacity in humans,” Journal of Agricultural Food Chemistry, vol. 51, no. 5, pp. 1500-1505, 2003.
  • R. A. Pérez, M. T. Iglesias, E. Pueyo, M. Gonzalez, C. de Lorenzo. “Amino acid composition and antioxidant capacity of Spanish honeys,” Journal of Agricultural Food Chemistry, Vol. 55, no. 2, pp.360-365, 2007.
  • S. K. Jaganathan, M. Mandal. “Honey constituents and their apoptotic effect in colon cancer cells,” Journal of ApiProduct ApiMedical Science, vol. 1, no. 2, pp. 29–36, 2009.
  • A. N. Fauzi, M. N. Norazmi, N. S. Yaacob. “Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines,” Food and Chemical Toxicology, Vol. 49, no. 4, pp. 871- 878, 2011.
  • R. B. Pimentel, C. A. de Q., da Costa, P. M. Albuquerque, S. D. Junior. “Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey,” BMC Complementary and Alternative Medicine, vol. 13, no. 1, pp. 1-14, 2013.
  • D. M. Borsato, A. S. Prudente, P. M. Döll-Boscardin, A. V. Borsato, C. F. P. Luz, B. H. L. N. S. Maia, O. G. Miguel. “Topical anti-Inflammatory activity of a monofloral honey of Mimosa scabrella provided by Melipona marginata during winter in Southern Brazil,” Journal of Medicinal Food., vol. 17, no. 7, pp. 817–825, 2014.
  • O. O. Erejuwa, S. A. Sulaiman, M. S. Wahab. “Effects of honey and its mechanisms of action on the development and progression of cancer,” Molecules, vol. 19, no. 2, pp. 2497-522, 2014.
  • P. M. Kustiawan, S. Puthong, E. T. Arung, C. Chanchao. “In vitro cytotoxicity of Indonesian stingless bee products against human cancer cell lines,” Asian Pacific Journal of Tropical Biomedicine, vol. 4, no. 7, pp. 549–556, 2014.
  • P. V. Rao, K. T. Krishnan, N. Salleh, S. H. Gan. “Biological and therapeutic effects of honey produced by honey bees and stingless bees: A comparative review,” Brazilian Journal of Pharmacognosy, vol. 26, no. 5, pp. 657–664, 2016.
  • M. S. A. Aziz, N. Giribabu, P. V. Rao, N. Salleh. “Pancreatoprotective effects of Geniotrigona thoracica stingless bee honey in streptozotocin-nicotinamide-induced male diabetic rats,” Biomedicine & Pharmacotherapy, vol. 89, 135–145, 2017.
  • P. Saranraj, S. Sivasakthi. “Comprehensive Review on Honey: Biochemical and Medicinal Properties,” Journal of Academia and Industrial Research, vol. 6, pp. 165–181, 2018.
  • V. C. Nolan, J. Harrison, J. A. G. Cox. “Dissecting the Antimicrobial Composition of Honey,” Antibiotics, vol. 8, no. 4, pp. 251, 2019.
  • P. C. Molan. “The antibacterial activity of honey: 1. The nature of the antibacterial activity,” Bee World, vol. 73, no. 1, pp. 5-28, 1992.
  • S. Anand, M. Deighton, G. Livanos, P. D. Morrison, E. C. K. Pang, N. Mantri. “Antimicrobial activity of Agastache Honey and characterization of its bioactive compounds in comparison with important commercial honeys,” Frontiers in Microbiology, vol. 10, pp. 269- 285, 2019.
  • V. Bansal, B. Medhi, P. Pandhi. “Honey–a remedy rediscovered and its therapeutic utility,” Kathmandu University Medical Journal, vol. 3, no. 3, pp. 305–309, 2005.
  • R. Wang, M. Starkey, R. Hazan, L. G. Rahme. “Honey’s ability to counter bacterial infections arises from both bactericidal compounds and QS inhibition,” Frontiers in Microbiology, vol. 3, pp. 144-150, 2012.
  • A. N. Koc, S. Silici, B. D. Ercal, F. Kasap, H. T. Hörmet- Öz, H. Mavus-Buldu. “Antifungal activity of Turkish honey against Candida spp. and Trichosporon spp: an in vitro evaluation,” Sabouraudia, vol. 47, no. 7, pp. 707-712, 2009.
  • M. Candiracci, B. Citterio, E. Piatti. “Antifungal activity of the honey flavonoid extract against Candida albicans,” Food Chemistry, vol. 131, no. 2, pp. 493 – 499, 2012.
  • H. Zafar, M. S. Israili. “Antimicrobial Properties of Honey,” American Journal of Therapeutics, vol. 21, no. 4, pp. 304-323, 2014.
  • J. M. Alvarez-Suarez, S. Tulipani, S. Diaz, Y. Estevez, S. Romandini, F. Giampieri, P. Damiani, S. Astolfi, S. Bompadre, M. Batting. “Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with colour, polyphenol content and others chemical compounds,” Food and Chemical Toxicology, vol. 48, pp. 2490- 2499, 2010.
  • Ö. Seçmen, Y. Gemici, G. Görk, L. Bekat, E. Leblebici. “Systematic of seeded plants,” Bornova, İzmir: Ege Üniversitesi, Faculty of Science Publications, vol. 116. pp. 396, 2000.
  • M. Balouiri, M. Sadiki, S. K. Ibnsouda. “Methods for in vitro evaluating antimicrobial activity: A review,” Journal of Pharmaceutical Analysis, vol. 6, no. 2, pp. 71-79, 2016.
  • I. Wiegand, K. Hilpert, R. E. Hancock. “Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances,” Nature Protocols., vol. 3, no. 2, pp. 163-175, 2008.
  • M. M. Mundo, O. I. Padılla-Zakour, R. W. Worobo. “Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys,” International Journal of Food Microbiology, vol. 97, pp. 1–8, 2004.
  • Ö. Ertürk, H. Şahin, S. Kolaylı, M. Ç. Ayvaz. “Antioxidant and antimicrobial activity of East Black Sea Region honeys,” Turkish Journal of Biochemistry/Turk Biyokimya Dergisi, vol. 39, no. 1, pp. 99-106, 2014.
  • J. Irish, D. A. Carter, T. Shokohi, S. E. Blair. Honey has an antifungal effect against Candida species,” Medical Mycology, vol. 44, no. 3, pp. 289-291, 2006.
  • M. D. Mandal, S. Mandal. Honey: its medicinal property and antibacterial activity,” Asian Pacific Journal of Tropical Biomedicine, vol. 1, no. 2, pp. 154-160, 2011.
  • P. J. Taormina, B. A. Niemira, L. R. Beuchat. “Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power,” International Journal of Food Microbiology, vol. 69, no. 3, pp. 217-225, 2001.
  • J. J. Gallardo-Chacón, M. Caselles, M. IzquierdoPulido, N. Rius. “Inhibitory activity of monofloral and multifloral honeys against bacterial pathogens,” Journal of Apicultural Research, vol. 47, no. 2, pp. 131-136, 2008.
  • K. L. Allen, P. C. Molan, G. M. Reid. “A survey of the antibacterial activity of some New Zealand honeys,” Journal of Pharmacy and Pharmacology,vol. 43, no. 12, pp. 817-822, 1991.
  • Jr. J. W. White, M. H. Subers, A. I. Schepartz. “The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system,” Biochimica et Biophysica Acta (BBA)- Specialized Section on Enzymological Subjects, vol. 73, no. 1, pp. 57-70, 1963.

Year 2023, Volume: 27 Issue: 4, 920 - 929, 25.08.2023

Mehtap Usta

https://doi.org/10.16984/saufenbilder.1284027

Abstract

References

  • S. Bogdanov, K. Ruoff, L. Persano Oddo. “Physico-chemical methods for the characterisation of unifloral honeys: A review,” Apidologie, vol. 35, no. 1, pp. 4-17, 2004.
  • E. Mahmoodi-Khaledi, J. Lozano-Sánchez, A. Bakhouche, M. Habibi-Rezaei, I. Sadeghian, A. Segura-Carretero. “Physicochemical properties and biological activities of honeys from different geographical and botanical origins in Iran,” European Food Research and Technology, vol. 243, no. 6, pp. 1019-1030, 2017.
  • S. Samarghandian, T. Farkhondeh, F. Samini. “Honey and Health: A Review of Recent Clinical Research,” Pharmacognosy Research, Vol. 9, no. 2, pp. 121-127, 2017.
  • F. J. Leyva-Jimenez, J. Lozano-Sanchez, I. BorrasLinares, M. L. Cadiz-Gurrea, E. MahmoodiKhaledi. “Potential antimicrobial activity of honey phenolic compounds against Gram positive and Gram negative bacteria,” LWT- Food Science and Technology, vol. 101, pp. 236-245, 2018.
  • E. I. Ramsay, S. Rao, L. Madathil, S. K. Hegde, M. P. Baliga-Rao, T. George, M. S. Baliga. “Honey in oral health and care: A mini review,” Journal of Oral Biosciences, vol. 61, pp. 32- 36, 2019.
  • N. Gheldof, X. H. Wang, N. J. Engeseth. “Buckwheat honey increases serum antioxidant capacity in humans,” Journal of Agricultural Food Chemistry, vol. 51, no. 5, pp. 1500-1505, 2003.
  • R. A. Pérez, M. T. Iglesias, E. Pueyo, M. Gonzalez, C. de Lorenzo. “Amino acid composition and antioxidant capacity of Spanish honeys,” Journal of Agricultural Food Chemistry, Vol. 55, no. 2, pp.360-365, 2007.
  • S. K. Jaganathan, M. Mandal. “Honey constituents and their apoptotic effect in colon cancer cells,” Journal of ApiProduct ApiMedical Science, vol. 1, no. 2, pp. 29–36, 2009.
  • A. N. Fauzi, M. N. Norazmi, N. S. Yaacob. “Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines,” Food and Chemical Toxicology, Vol. 49, no. 4, pp. 871- 878, 2011.
  • R. B. Pimentel, C. A. de Q., da Costa, P. M. Albuquerque, S. D. Junior. “Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey,” BMC Complementary and Alternative Medicine, vol. 13, no. 1, pp. 1-14, 2013.
  • D. M. Borsato, A. S. Prudente, P. M. Döll-Boscardin, A. V. Borsato, C. F. P. Luz, B. H. L. N. S. Maia, O. G. Miguel. “Topical anti-Inflammatory activity of a monofloral honey of Mimosa scabrella provided by Melipona marginata during winter in Southern Brazil,” Journal of Medicinal Food., vol. 17, no. 7, pp. 817–825, 2014.
  • O. O. Erejuwa, S. A. Sulaiman, M. S. Wahab. “Effects of honey and its mechanisms of action on the development and progression of cancer,” Molecules, vol. 19, no. 2, pp. 2497-522, 2014.
  • P. M. Kustiawan, S. Puthong, E. T. Arung, C. Chanchao. “In vitro cytotoxicity of Indonesian stingless bee products against human cancer cell lines,” Asian Pacific Journal of Tropical Biomedicine, vol. 4, no. 7, pp. 549–556, 2014.
  • P. V. Rao, K. T. Krishnan, N. Salleh, S. H. Gan. “Biological and therapeutic effects of honey produced by honey bees and stingless bees: A comparative review,” Brazilian Journal of Pharmacognosy, vol. 26, no. 5, pp. 657–664, 2016.
  • M. S. A. Aziz, N. Giribabu, P. V. Rao, N. Salleh. “Pancreatoprotective effects of Geniotrigona thoracica stingless bee honey in streptozotocin-nicotinamide-induced male diabetic rats,” Biomedicine & Pharmacotherapy, vol. 89, 135–145, 2017.
  • P. Saranraj, S. Sivasakthi. “Comprehensive Review on Honey: Biochemical and Medicinal Properties,” Journal of Academia and Industrial Research, vol. 6, pp. 165–181, 2018.
  • V. C. Nolan, J. Harrison, J. A. G. Cox. “Dissecting the Antimicrobial Composition of Honey,” Antibiotics, vol. 8, no. 4, pp. 251, 2019.
  • P. C. Molan. “The antibacterial activity of honey: 1. The nature of the antibacterial activity,” Bee World, vol. 73, no. 1, pp. 5-28, 1992.
  • S. Anand, M. Deighton, G. Livanos, P. D. Morrison, E. C. K. Pang, N. Mantri. “Antimicrobial activity of Agastache Honey and characterization of its bioactive compounds in comparison with important commercial honeys,” Frontiers in Microbiology, vol. 10, pp. 269- 285, 2019.
  • V. Bansal, B. Medhi, P. Pandhi. “Honey–a remedy rediscovered and its therapeutic utility,” Kathmandu University Medical Journal, vol. 3, no. 3, pp. 305–309, 2005.
  • R. Wang, M. Starkey, R. Hazan, L. G. Rahme. “Honey’s ability to counter bacterial infections arises from both bactericidal compounds and QS inhibition,” Frontiers in Microbiology, vol. 3, pp. 144-150, 2012.
  • A. N. Koc, S. Silici, B. D. Ercal, F. Kasap, H. T. Hörmet- Öz, H. Mavus-Buldu. “Antifungal activity of Turkish honey against Candida spp. and Trichosporon spp: an in vitro evaluation,” Sabouraudia, vol. 47, no. 7, pp. 707-712, 2009.
  • M. Candiracci, B. Citterio, E. Piatti. “Antifungal activity of the honey flavonoid extract against Candida albicans,” Food Chemistry, vol. 131, no. 2, pp. 493 – 499, 2012.
  • H. Zafar, M. S. Israili. “Antimicrobial Properties of Honey,” American Journal of Therapeutics, vol. 21, no. 4, pp. 304-323, 2014.
  • J. M. Alvarez-Suarez, S. Tulipani, S. Diaz, Y. Estevez, S. Romandini, F. Giampieri, P. Damiani, S. Astolfi, S. Bompadre, M. Batting. “Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with colour, polyphenol content and others chemical compounds,” Food and Chemical Toxicology, vol. 48, pp. 2490- 2499, 2010.
  • Ö. Seçmen, Y. Gemici, G. Görk, L. Bekat, E. Leblebici. “Systematic of seeded plants,” Bornova, İzmir: Ege Üniversitesi, Faculty of Science Publications, vol. 116. pp. 396, 2000.
  • M. Balouiri, M. Sadiki, S. K. Ibnsouda. “Methods for in vitro evaluating antimicrobial activity: A review,” Journal of Pharmaceutical Analysis, vol. 6, no. 2, pp. 71-79, 2016.
  • I. Wiegand, K. Hilpert, R. E. Hancock. “Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances,” Nature Protocols., vol. 3, no. 2, pp. 163-175, 2008.
  • M. M. Mundo, O. I. Padılla-Zakour, R. W. Worobo. “Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys,” International Journal of Food Microbiology, vol. 97, pp. 1–8, 2004.
  • Ö. Ertürk, H. Şahin, S. Kolaylı, M. Ç. Ayvaz. “Antioxidant and antimicrobial activity of East Black Sea Region honeys,” Turkish Journal of Biochemistry/Turk Biyokimya Dergisi, vol. 39, no. 1, pp. 99-106, 2014.
  • J. Irish, D. A. Carter, T. Shokohi, S. E. Blair. Honey has an antifungal effect against Candida species,” Medical Mycology, vol. 44, no. 3, pp. 289-291, 2006.
  • M. D. Mandal, S. Mandal. Honey: its medicinal property and antibacterial activity,” Asian Pacific Journal of Tropical Biomedicine, vol. 1, no. 2, pp. 154-160, 2011.
  • P. J. Taormina, B. A. Niemira, L. R. Beuchat. “Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power,” International Journal of Food Microbiology, vol. 69, no. 3, pp. 217-225, 2001.
  • J. J. Gallardo-Chacón, M. Caselles, M. IzquierdoPulido, N. Rius. “Inhibitory activity of monofloral and multifloral honeys against bacterial pathogens,” Journal of Apicultural Research, vol. 47, no. 2, pp. 131-136, 2008.
  • K. L. Allen, P. C. Molan, G. M. Reid. “A survey of the antibacterial activity of some New Zealand honeys,” Journal of Pharmacy and Pharmacology,vol. 43, no. 12, pp. 817-822, 1991.
  • Jr. J. W. White, M. H. Subers, A. I. Schepartz. “The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system,” Biochimica et Biophysica Acta (BBA)- Specialized Section on Enzymological Subjects, vol. 73, no. 1, pp. 57-70, 1963.
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Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Mehtap Usta 0000-0001-7656-5655

Early Pub Date August 19, 2023
Publication Date August 25, 2023
Submission Date April 15, 2023
Acceptance Date June 5, 2023
Published in Issue Year 2023 Volume: 27 Issue: 4

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APA Usta, M. (2023). Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey. Sakarya University Journal of Science, 27(4), 920-929. https://doi.org/10.16984/saufenbilder.1284027
AMA Usta M. Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey. SAUJS. August 2023;27(4):920-929. doi:10.16984/saufenbilder.1284027
Chicago Usta, Mehtap. “Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey”. Sakarya University Journal of Science 27, no. 4 (August 2023): 920-29. https://doi.org/10.16984/saufenbilder.1284027.
EndNote Usta M (August 1, 2023) Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey. Sakarya University Journal of Science 27 4 920–929.
IEEE M. Usta, “Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey”, SAUJS, vol. 27, no. 4, pp. 920–929, 2023, doi: 10.16984/saufenbilder.1284027.
ISNAD Usta, Mehtap. “Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey”. Sakarya University Journal of Science 27/4 (August 2023), 920-929. https://doi.org/10.16984/saufenbilder.1284027.
JAMA Usta M. Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey. SAUJS. 2023;27:920–929.
MLA Usta, Mehtap. “Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey”. Sakarya University Journal of Science, vol. 27, no. 4, 2023, pp. 920-9, doi:10.16984/saufenbilder.1284027.
Vancouver Usta M. Survay Study of Antimicrobial Activities of Different Region Honeys in Turkey. SAUJS. 2023;27(4):920-9.
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