Research Article
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Year 2021, Volume: 25 Issue: 3, 788 - 799, 30.06.2021

Latife Ceyda İrkin Şamil Öztürk

https://doi.org/10.16984/saufenbilder.886245
Cited By: 1

Abstract

Supporting Institution

Trakya Üniversitesi Bilimsel Araştırmalar Proje Birimi

Project Number

TÜBAB/2011-125

References

  • [1] A. Aktümsek, “Anatomi ve fizyoloji insan biyolojisi,” Nobel Yayınları, Ankara, 3th edt, pp. 366-7, 2006.
  • [2] T. E. Andreoli, “Liver,” In M. B. Fallon, B. M. McGuire, G. A. Abrams, M. R. Arguedas (ed), Cecil essentials of medicine, 5th edt. WB Saunders Company, Philadelpia, pp. 365-98, 2001.
  • [3] N. Fausto, “Liver regeneration,” Journal of Hepatol, vol. 32, pp. 19-31, 2000.
  • [4] M. Basoglu, A. A. Balık, R. Kavak, C. Gundogdu, Z. Erman, R. Yıldırgan, D. Oren, “Effects of growth hormone on hepatic regeneration,” Turk J Med Sci., vol. 30, pp. 529-34, 2000.
  • [5] Z. G. Ren, J. D. Zhao, K. Gu, J. Wang, G. L. Jiang, “Hepatic proliferation after partial liver irradiation in Sprague-Dawley rats,” Mol. Biol. Rep., vol. 39, pp. 3829-36, 2012.
  • [6] E. A. Pomfret, J. J. Pomposelli, F. D. Gordon, N. Erbay, L. Lyn, W. D. Lewis, “Liver regeneration and surgical outcome in donors of right-lobe liver grafts,” Transplantation, vol. 76, pp. 5-10, 2003.
  • [7] T. Nakamura, S. Mizuno, “The discovery of hepatocyte growth factor (HGF) and its significance for cell biology, life sciences and clinical medicine,” Proc Jpn Acad Ser B Phys Biol Sci., vol. 86, no:6, pp. 588-610, 2010.
  • [8] S. Perek, S. Kapan, “Cerrahi Gastroenteroloji,” In U. Değerli, Y. Bozfakıoğlu (ed), İstanbul, Nobel Tıp Kitabevleri, vol. 5, pp. 194-208, 2000.
  • [9] G. Garcea, G. J. Maddern, “Liver failure after major hepatic resection,” J Hepatobiliary Pancreat Surg., vol. 16, pp. 145-155, 2009.
  • [10] G. K. Michalopoulos, M. C. DeFrances, “Liver regeneration”, Science, vol. 296, pp. 60-6, 1996.
  • [11] L. Chen, H. L. Sun, L. Yang, H. J. Du, W. An, “Transfection of human hepatic stimulator substance gene could protect BEL-7402 cells against hepatotoxins,” Zhonghua Gan Zang Bing Za Zhi., vol. 12, no:2, pp. 99-101, 2004.
  • [12] H. Y. Hsu, M. C. Yu, C. W. Lee, “RAM score is an effective predictor for early mortality and recurrence after hepatectomy for hepatocellular carcinoma,” BMC Cancer, vol. 17, no:1, pp. 742, 2017.
  • [13] S. Iwatsuki, B. W. Shaw, T. E. Jr Starzi, “Experience with 150 liver resections,” Ann Surg.,vol. 197, pp. 247-52, 1983.
  • [14] Canbek, M. Uyanoglu, S. Canbek, E. Ceyhan, A. Ozen, B. Durmus, O Turgak, “The effect of geraniol on liver regeneration after hepatectomy in rats,” In Vivo, vol. 31, no: 2, pp. 209-213, 2017.
  • [15] M. Bouattour, E. Raymond, S. Qin, A. L. Cheng, U. Stammberger, G. Locatelli, S. Faivre, “Recent developments of c-Met as a therapeutic target in hepatocellular carcinoma,” Hepatology, vol. 67, no: 3, pp. 1132-1149, 2018.
  • [16] M. J. Xu, D. Feng, H. Wu, H. Wang, Y. Chan, J. Kolls, N. Borregaard, B. Porse, T. Berger, T. W. Mak, J. B. Cowland, X. Kong, B Gao, “Liver is the major source of elevated serum lipocalin-2 levels after bacterial infection or partial hepatectomy: a critical role for IL-6/STAT3,” Hepatology, vol. 61, no: 2, pp. 692-702, 2015.
  • [17] R. Bönninghoff, K. Schwenke, M. Keese, R. Magdeburg, H. Bitter-Suermann, M. Otto, T. Hasenberg, S. Post, J. Sturm, “Effect of different liver resection methods on liver damage and regeneration factors VEGF and FGF-2 in mice,” Can J Surg., vol. 55, no: 6, pp. 389-93, 2012.
  • [18] R. Yang, T. Uchiyama, S. K. Watkins, X. Han, M. P. Fink, “Ethyl pyruvate reduces liver injury in a murine model of extrahepatic cholestasis,” Shock, vol. 22, pp. 369-75, 2004.
  • [19] N. Ekici Günay, S. Muhtaroğlu, A. Bedirli, “Administration of Ginkgo biloba extract (egb761) alone and in combination with fk506 promotes liver regeneration in a rat model of partial hepatectomy,” Balkan Med J., vol. 35, no: 2, pp. 174-180, 2018.
  • [20] K. Kagure, Y. Q. Zhang, H. Shibata, I. Kojima, “Immediate onset of DNA synthesis in remnant rat liver after %90 hepatectomy by an administration of follistatin,” J Hepatol., vol. 28, no: 3, pp. 977-984, 1998.
  • [21] M. Selzner, P.A. Clavien, “Failure of regeneration of the steatotic rat liver: Disruption at two different levels in the regeneration pathway,” Hepatology, vol. 31, pp. 35-42, 2000.
  • [22] R. G. Kilbourn, D. L. Traber, C. Szabo, “Nitrik oxide and shock,” Dis Mon., vol. 47, no: 5, pp. 277-348, 1997.
  • [23] H. Hayashi, M. Nagaki, M. Imose, Y. Osawa, K. Kimura, S. Takai, M. Imao, T. Naiki, T. Kato, H. Moriwaki, “Normal liver regeneration and liver cell apoptosis after partial hepatectomy in tumor necrosis factor-alpha-deficient mice,” Liver Int., vol. 25, no: 1, pp. 162-70, 2005.
  • [24] J. A. Ebrenfried, T. C. Ko, E. A. Thompson, B. M. Evers, “Cell cycle-mediated regulation of hepatic regeneration,” Surgery, vol. 122, no: 5, pp. 927-35, 1997.
  • [25] R. Veteläinen, A. K. van Vliet, T. M. van Gulik, “Severe steatosis increases hepatocellular injury and impairs liver regeneration in a rat model of partial hepatectomy,” Ann Surg., vol. 245, pp. 44-50, 2007.
  • [26] D. Palmes, H. U. Spiegel, “Animal models of liver regeneration,” Biomaterials, vol. 25, no: 9, pp.1601-11, 2004.
  • [27] W. C. Meyers, R. S. Jones, “Anatomy,” In W. C. Meyers, R. S. Jones (eds), Textbook of liver and biliary surgery, Press: JB Lippincott Company, Philadelphia, pp. 18–38, 1990.
  • [28] F. G. Court, S. A. Wemyss-Holden, R. A. Dennison, G. J. Maddern, “The mystery of liver regeneration,” Br J Surg., vol. 89, pp. 1089-95, 2002.
  • [29] J. Kountouras, P. Boura, N. J. Lygidakis, “Liver regeneration after hepatectomy,” Hepatogastroenterology, vol. 48, no: 38, pp. 556-62, 2001.
  • [30] N. Fausto, “Hepatic regeneration,” D. Zakim, T. D. Boyer Eds., Press: Hepatology WB Saunders, Philadelphia, pp. 32-58, 1998.
  • [31] S. T. Fan, C. M. Lo, C. L. Liu, C. M. Lam, “Hepatectomy for hepatocellular carcinoma toward zero hospital deaths,” Ann Surg., vol. 229, pp. 322-30, 1999.
  • [32] N. Assy, G. Y. Minuk, “Liver regeneration: methods for monitoring and their applications,” J Hepatol., vol. 26, pp. 945-52, 1997.
  • [33] C. Picard, L. Lambotte, P. Starkel, C. Sempoux, A. Saliez, V. V. D. Berge, “Steatosis is not sufficient to cause an impaired regenerative response after partial hepatectomy in rats,” J Hepatol., vol.32, pp. 645-52, 2002.
  • [34] J. O. Castro-e-Silva, S. Zucoloto, F. S. Ramalho, L. N. Z. Ramalho, J. M. C. Reis, A. A. C. Bastos, M. V. H. Brito, “Antiproliferative activity of Copaifera duckei oleoresin on liver regeneration in rats,” Phytother Res., vol. 18, pp. 92-94, 2004.
  • [35] E. Kamer, A. Çöker, A. R. Sevinç, E. Özkara, E. Özer, T. Özzeybek, “Effect of intraperitoneal dministration of gemcitabine and paclitaxel on hepatic regeneration in rats,” Turk J Gastroenterol., vol. 14, no: 1, pp. 1-6, 2003.
  • [36] A. Akcan, “The effect of amrinone on liver regeneration in experimental hepatic resection model,” J Surg Res., vol. 130, pp. 66–72, 2006.
  • [37] J. P. Sowa, J. Best, T. Benko, M. Bockhorn, Y. Gu, E. M. Niehues, “Extent of liver resection modulates the activation of transcription factors and the production of cytokines involved in liver regeneration,” World J Gastroenterol., vol. 14, no: 46, pp. 7093-100, 2008.

Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index

Year 2021, Volume: 25 Issue: 3, 788 - 799, 30.06.2021

Latife Ceyda İrkin Şamil Öztürk

https://doi.org/10.16984/saufenbilder.886245
Cited By: 1

Abstract

Liver regeneration is known to begin after experimentally induced liver resection. At first 24-48 hours, DNA synthesis reaches the maximum level after resection and significantly regeneration known to occur on the first 10 days. In this study, we was examined the regeneration of liver on the 1st and 14th days after the resection.
In experiment 21 Wistar albino male rats were used. The rats were randomly divided into three groups. All experimentally groups realized midline incision with laparotomy for resection of liver. Pedicles of the left lateral and median lobes of the liver were applied 70% hepatectomy by 4/0 silk binding. At the end of 1st and 14th days, liver tissue removed for light misroscobic analysis.
The tissue of all experimentally groups were showed some histopatological changes such as sinuzoidal dilatation, vacuolization in the hepatocytes. This histopatological findings were seem to increase at II. grup more than experimentally groups.
Mitotic index, apoptotic index and proliferation index of values at II. group was reached the maximum level. Other group (III) dramatically reduced the value of these index and were seem to reach near to values of the control group. Relative liver weight that determined each of experimentally groups were statistically significant differences compared to the control group.

Keywords

Partial hepatectomy TUNEL Apotosis Immunohistochemistry PCNA

Project Number

TÜBAB/2011-125

References

  • [1] A. Aktümsek, “Anatomi ve fizyoloji insan biyolojisi,” Nobel Yayınları, Ankara, 3th edt, pp. 366-7, 2006.
  • [2] T. E. Andreoli, “Liver,” In M. B. Fallon, B. M. McGuire, G. A. Abrams, M. R. Arguedas (ed), Cecil essentials of medicine, 5th edt. WB Saunders Company, Philadelpia, pp. 365-98, 2001.
  • [3] N. Fausto, “Liver regeneration,” Journal of Hepatol, vol. 32, pp. 19-31, 2000.
  • [4] M. Basoglu, A. A. Balık, R. Kavak, C. Gundogdu, Z. Erman, R. Yıldırgan, D. Oren, “Effects of growth hormone on hepatic regeneration,” Turk J Med Sci., vol. 30, pp. 529-34, 2000.
  • [5] Z. G. Ren, J. D. Zhao, K. Gu, J. Wang, G. L. Jiang, “Hepatic proliferation after partial liver irradiation in Sprague-Dawley rats,” Mol. Biol. Rep., vol. 39, pp. 3829-36, 2012.
  • [6] E. A. Pomfret, J. J. Pomposelli, F. D. Gordon, N. Erbay, L. Lyn, W. D. Lewis, “Liver regeneration and surgical outcome in donors of right-lobe liver grafts,” Transplantation, vol. 76, pp. 5-10, 2003.
  • [7] T. Nakamura, S. Mizuno, “The discovery of hepatocyte growth factor (HGF) and its significance for cell biology, life sciences and clinical medicine,” Proc Jpn Acad Ser B Phys Biol Sci., vol. 86, no:6, pp. 588-610, 2010.
  • [8] S. Perek, S. Kapan, “Cerrahi Gastroenteroloji,” In U. Değerli, Y. Bozfakıoğlu (ed), İstanbul, Nobel Tıp Kitabevleri, vol. 5, pp. 194-208, 2000.
  • [9] G. Garcea, G. J. Maddern, “Liver failure after major hepatic resection,” J Hepatobiliary Pancreat Surg., vol. 16, pp. 145-155, 2009.
  • [10] G. K. Michalopoulos, M. C. DeFrances, “Liver regeneration”, Science, vol. 296, pp. 60-6, 1996.
  • [11] L. Chen, H. L. Sun, L. Yang, H. J. Du, W. An, “Transfection of human hepatic stimulator substance gene could protect BEL-7402 cells against hepatotoxins,” Zhonghua Gan Zang Bing Za Zhi., vol. 12, no:2, pp. 99-101, 2004.
  • [12] H. Y. Hsu, M. C. Yu, C. W. Lee, “RAM score is an effective predictor for early mortality and recurrence after hepatectomy for hepatocellular carcinoma,” BMC Cancer, vol. 17, no:1, pp. 742, 2017.
  • [13] S. Iwatsuki, B. W. Shaw, T. E. Jr Starzi, “Experience with 150 liver resections,” Ann Surg.,vol. 197, pp. 247-52, 1983.
  • [14] Canbek, M. Uyanoglu, S. Canbek, E. Ceyhan, A. Ozen, B. Durmus, O Turgak, “The effect of geraniol on liver regeneration after hepatectomy in rats,” In Vivo, vol. 31, no: 2, pp. 209-213, 2017.
  • [15] M. Bouattour, E. Raymond, S. Qin, A. L. Cheng, U. Stammberger, G. Locatelli, S. Faivre, “Recent developments of c-Met as a therapeutic target in hepatocellular carcinoma,” Hepatology, vol. 67, no: 3, pp. 1132-1149, 2018.
  • [16] M. J. Xu, D. Feng, H. Wu, H. Wang, Y. Chan, J. Kolls, N. Borregaard, B. Porse, T. Berger, T. W. Mak, J. B. Cowland, X. Kong, B Gao, “Liver is the major source of elevated serum lipocalin-2 levels after bacterial infection or partial hepatectomy: a critical role for IL-6/STAT3,” Hepatology, vol. 61, no: 2, pp. 692-702, 2015.
  • [17] R. Bönninghoff, K. Schwenke, M. Keese, R. Magdeburg, H. Bitter-Suermann, M. Otto, T. Hasenberg, S. Post, J. Sturm, “Effect of different liver resection methods on liver damage and regeneration factors VEGF and FGF-2 in mice,” Can J Surg., vol. 55, no: 6, pp. 389-93, 2012.
  • [18] R. Yang, T. Uchiyama, S. K. Watkins, X. Han, M. P. Fink, “Ethyl pyruvate reduces liver injury in a murine model of extrahepatic cholestasis,” Shock, vol. 22, pp. 369-75, 2004.
  • [19] N. Ekici Günay, S. Muhtaroğlu, A. Bedirli, “Administration of Ginkgo biloba extract (egb761) alone and in combination with fk506 promotes liver regeneration in a rat model of partial hepatectomy,” Balkan Med J., vol. 35, no: 2, pp. 174-180, 2018.
  • [20] K. Kagure, Y. Q. Zhang, H. Shibata, I. Kojima, “Immediate onset of DNA synthesis in remnant rat liver after %90 hepatectomy by an administration of follistatin,” J Hepatol., vol. 28, no: 3, pp. 977-984, 1998.
  • [21] M. Selzner, P.A. Clavien, “Failure of regeneration of the steatotic rat liver: Disruption at two different levels in the regeneration pathway,” Hepatology, vol. 31, pp. 35-42, 2000.
  • [22] R. G. Kilbourn, D. L. Traber, C. Szabo, “Nitrik oxide and shock,” Dis Mon., vol. 47, no: 5, pp. 277-348, 1997.
  • [23] H. Hayashi, M. Nagaki, M. Imose, Y. Osawa, K. Kimura, S. Takai, M. Imao, T. Naiki, T. Kato, H. Moriwaki, “Normal liver regeneration and liver cell apoptosis after partial hepatectomy in tumor necrosis factor-alpha-deficient mice,” Liver Int., vol. 25, no: 1, pp. 162-70, 2005.
  • [24] J. A. Ebrenfried, T. C. Ko, E. A. Thompson, B. M. Evers, “Cell cycle-mediated regulation of hepatic regeneration,” Surgery, vol. 122, no: 5, pp. 927-35, 1997.
  • [25] R. Veteläinen, A. K. van Vliet, T. M. van Gulik, “Severe steatosis increases hepatocellular injury and impairs liver regeneration in a rat model of partial hepatectomy,” Ann Surg., vol. 245, pp. 44-50, 2007.
  • [26] D. Palmes, H. U. Spiegel, “Animal models of liver regeneration,” Biomaterials, vol. 25, no: 9, pp.1601-11, 2004.
  • [27] W. C. Meyers, R. S. Jones, “Anatomy,” In W. C. Meyers, R. S. Jones (eds), Textbook of liver and biliary surgery, Press: JB Lippincott Company, Philadelphia, pp. 18–38, 1990.
  • [28] F. G. Court, S. A. Wemyss-Holden, R. A. Dennison, G. J. Maddern, “The mystery of liver regeneration,” Br J Surg., vol. 89, pp. 1089-95, 2002.
  • [29] J. Kountouras, P. Boura, N. J. Lygidakis, “Liver regeneration after hepatectomy,” Hepatogastroenterology, vol. 48, no: 38, pp. 556-62, 2001.
  • [30] N. Fausto, “Hepatic regeneration,” D. Zakim, T. D. Boyer Eds., Press: Hepatology WB Saunders, Philadelphia, pp. 32-58, 1998.
  • [31] S. T. Fan, C. M. Lo, C. L. Liu, C. M. Lam, “Hepatectomy for hepatocellular carcinoma toward zero hospital deaths,” Ann Surg., vol. 229, pp. 322-30, 1999.
  • [32] N. Assy, G. Y. Minuk, “Liver regeneration: methods for monitoring and their applications,” J Hepatol., vol. 26, pp. 945-52, 1997.
  • [33] C. Picard, L. Lambotte, P. Starkel, C. Sempoux, A. Saliez, V. V. D. Berge, “Steatosis is not sufficient to cause an impaired regenerative response after partial hepatectomy in rats,” J Hepatol., vol.32, pp. 645-52, 2002.
  • [34] J. O. Castro-e-Silva, S. Zucoloto, F. S. Ramalho, L. N. Z. Ramalho, J. M. C. Reis, A. A. C. Bastos, M. V. H. Brito, “Antiproliferative activity of Copaifera duckei oleoresin on liver regeneration in rats,” Phytother Res., vol. 18, pp. 92-94, 2004.
  • [35] E. Kamer, A. Çöker, A. R. Sevinç, E. Özkara, E. Özer, T. Özzeybek, “Effect of intraperitoneal dministration of gemcitabine and paclitaxel on hepatic regeneration in rats,” Turk J Gastroenterol., vol. 14, no: 1, pp. 1-6, 2003.
  • [36] A. Akcan, “The effect of amrinone on liver regeneration in experimental hepatic resection model,” J Surg Res., vol. 130, pp. 66–72, 2006.
  • [37] J. P. Sowa, J. Best, T. Benko, M. Bockhorn, Y. Gu, E. M. Niehues, “Extent of liver resection modulates the activation of transcription factors and the production of cytokines involved in liver regeneration,” World J Gastroenterol., vol. 14, no: 46, pp. 7093-100, 2008.
There are 37 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Latife Ceyda İrkin 0000-0001-6603-8413

Şamil Öztürk 0000-0002-9435-8139

Project Number TÜBAB/2011-125
Publication Date June 30, 2021
Submission Date February 24, 2021
Acceptance Date May 3, 2021
Published in Issue Year 2021 Volume: 25 Issue: 3

Cite

APA İrkin, L. C., & Öztürk, Ş. (2021). Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index. Sakarya University Journal of Science, 25(3), 788-799. https://doi.org/10.16984/saufenbilder.886245
AMA İrkin LC, Öztürk Ş. Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index. SAUJS. June 2021;25(3):788-799. doi:10.16984/saufenbilder.886245
Chicago İrkin, Latife Ceyda, and Şamil Öztürk. “Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index”. Sakarya University Journal of Science 25, no. 3 (June 2021): 788-99. https://doi.org/10.16984/saufenbilder.886245.
EndNote İrkin LC, Öztürk Ş (June 1, 2021) Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index. Sakarya University Journal of Science 25 3 788–799.
IEEE L. C. İrkin and Ş. Öztürk, “Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index”, SAUJS, vol. 25, no. 3, pp. 788–799, 2021, doi: 10.16984/saufenbilder.886245.
ISNAD İrkin, Latife Ceyda - Öztürk, Şamil. “Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index”. Sakarya University Journal of Science 25/3 (June 2021), 788-799. https://doi.org/10.16984/saufenbilder.886245.
JAMA İrkin LC, Öztürk Ş. Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index. SAUJS. 2021;25:788–799.
MLA İrkin, Latife Ceyda and Şamil Öztürk. “Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index”. Sakarya University Journal of Science, vol. 25, no. 3, 2021, pp. 788-99, doi:10.16984/saufenbilder.886245.
Vancouver İrkin LC, Öztürk Ş. Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index. SAUJS. 2021;25(3):788-99.

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