Pinus sylvestris var. hamata Uçucu Yağlarının Fitokimyasal Bileşimi, Antioksidan ve Antimikrobiyal Aktiviteleri
Abstract
Çamgiller familyasına ait olan Pinus slyvestris L., değerli orman ağaçlarından biridir. Çam yağları, içecek ve yiyeceklerde tatlandırıcı olarak ve kozmetikte koku olarak kullanılır. Pinus sylvestris var. hamata Türkiye'nin Orta Karadeniz Bölgesi'nden toplanmış ve iğneleri gölgede kurutulmuştur. Uçucu yağlar (EO'lar) hidrodistilasyonla üretildi ve EO bileşiklerini tanımlamak için Gaz kromatografisi/kütle spektrofotometrisi (GC-MS/MS) kullanıldı. β-Pinene (%18,70), α-Pinene (%15,62), Germacrene (%12,53), Karyofilen (%11,35) ve Limonen (%3,62) ana bileşenlerdi. EO'ların antioksidan analizi için DPPH•, ABTS+• süpürme ve FRAP testleri yapıldı. Ayrıca, antibakteriyel etki, Bacillus cereus, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae ve Staphylococcus aureus ve kullanılarak disk difüzyon testi ile araştırılmıştır. EO'lar, Salmonella typhimurium dışında test edilen bakterilere karşı iyi antibakteriyel aktiviteler sergiledi. Ek olarak, EO'lar, test edilen deneylerde önemli aktiviteyi ortaya çıkardı. Sonuç olarak, P. sylveris var. hamata esansiyel yağının ilaç ve gıda endüstrisinde kullanılması mümkündür.
Supporting Institution
Tübitak 2209
Project Number
1919B012100875
References
- Abi-Ayad M., Abi-Ayad FZ., Lazzouni HA., Rebiahi SA. Antibacterial activity of Pinus halepensis essential oil from Algeria (Tlemcen). Journal of Natural Product and Plant Resources 2J Nat Prod Plant Resour 2011; 1(1): 33-36.
- Al-Breiki AM., Al-Brashdi HM., Al-Sabahi JN., Khan SA. Comparative GC-MS analysis, in-vitro antioxidant and antimicrobial activities of the essential oils isolated from the peel of Omani lime. Chiang Mai Journal of Science 2018; 45(4): 1782-1795.
- Allenspach M., Valder C., Flamm D., Grisoni F., Steuer C. Verification of chromatographic profile of primary essential oil of Pinus sylvestris L. combined with chemometric analysis. Molecules 2020; 25(13): 2973.
- Ayad R., Akkal S. Phytochemistry and biological activities of algerian Centaurea and related genera. Studies in Natural Products Chemistry 2019; 63: 357-414.
- Barnola L., Cedeño A. Inter-population differences in the essential oils of Pinus caribaea needles. Biochemical Systematics and Ecology 2000; 28(10): 923-931.
- Bassolé IHN., Juliani HR. Essential oils in combination and their antimicrobial properties. Molecules 2012; 17(4): 3989-4006.
- Botsoglou N., Florou-Paneri P., Christaki E., Fletouris D., Spais A. Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues. British Poultry Science 2002; 43(2): 223-230.
- Burt S. Essential oils: their antibacterial properties and potential applications in foods—A review. International Journal of Food Microbiology 2004; 94(3): 223-253.
- Chao SC., Young DG., Oberg CJ. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research 2000; 12(5): 639-649.
- Dahham SS., Tabana YM., Iqbal MA., Ahamed MB., Ezzat MO., Majid AS., Majid AM.et al. The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna. Molecules 2015; 20(7): 11808-11829.
- Doughari JH., Bazza MJ. Phytochemistry, GC-MS analysis, antioxidant and antibacterial potentials of limonene isolated from pericarp of Citrus sinensis. International Journal of Microbiology and Biotechnology 2010; 5: 22-27.
- Erenler R., Tugba A., Karan T., Elmastas M., Yildiz I., Aksit H., Topcu G., Sanda MA. et al. Chemical constituents isolated from Origanum solymicum with antioxidant activitites. The Eurasia Proceedings of Science Technology Engineering and Mathematics 2017; 1: 139-145.
Phytochemical Composition, Antioxidant And Antimicrobial Activities of Essential Oils of Pinus sylvestris var. hamata
Abstract
Pinus slyvestris L., which belongs to the Pinaceae family, is one of valuable forest trees. Pine oils are used as a flavoring in beverages and food and as a fragrance in cosmetics. Pinus sylvestris var hamata was collected from the Central Black Sea Region of Turkey and its needles were dried in the shade. Essential oils (EOs) was generated by hydrodistillation and Gas chromatography/mass spectrophotometry (GC-MS/MS) was used to identifiy the EOs compounds. β-Pinene (18.70%), α-Pinene (15.62%), Germacrene (12.53%), Caryophyllene (11.35%) and Limonene (3.62%) were major constituents. DPPH• ABTS+• scavenging and FRAP tests were performed for antioxidant analysis of EOs. Morever, antibacterial effect was investigated by disk diffusion assay using Bacillus cereus, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus and. EOs exhibited good antibacterial activities against the tested bacteria, except for Salmonella typhimurium. In addition EOs revealed the significant activity on tested assays. As results, P. sylveris var. hamata essential oil has possible to be utilized in the pharmaceutical and food industries
Project Number
1919B012100875
References
- Abi-Ayad M., Abi-Ayad FZ., Lazzouni HA., Rebiahi SA. Antibacterial activity of Pinus halepensis essential oil from Algeria (Tlemcen). Journal of Natural Product and Plant Resources 2J Nat Prod Plant Resour 2011; 1(1): 33-36.
- Al-Breiki AM., Al-Brashdi HM., Al-Sabahi JN., Khan SA. Comparative GC-MS analysis, in-vitro antioxidant and antimicrobial activities of the essential oils isolated from the peel of Omani lime. Chiang Mai Journal of Science 2018; 45(4): 1782-1795.
- Allenspach M., Valder C., Flamm D., Grisoni F., Steuer C. Verification of chromatographic profile of primary essential oil of Pinus sylvestris L. combined with chemometric analysis. Molecules 2020; 25(13): 2973.
- Ayad R., Akkal S. Phytochemistry and biological activities of algerian Centaurea and related genera. Studies in Natural Products Chemistry 2019; 63: 357-414.
- Barnola L., Cedeño A. Inter-population differences in the essential oils of Pinus caribaea needles. Biochemical Systematics and Ecology 2000; 28(10): 923-931.
- Bassolé IHN., Juliani HR. Essential oils in combination and their antimicrobial properties. Molecules 2012; 17(4): 3989-4006.
- Botsoglou N., Florou-Paneri P., Christaki E., Fletouris D., Spais A. Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues. British Poultry Science 2002; 43(2): 223-230.
- Burt S. Essential oils: their antibacterial properties and potential applications in foods—A review. International Journal of Food Microbiology 2004; 94(3): 223-253.
- Chao SC., Young DG., Oberg CJ. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research 2000; 12(5): 639-649.
- Dahham SS., Tabana YM., Iqbal MA., Ahamed MB., Ezzat MO., Majid AS., Majid AM.et al. The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna. Molecules 2015; 20(7): 11808-11829.
- Doughari JH., Bazza MJ. Phytochemistry, GC-MS analysis, antioxidant and antibacterial potentials of limonene isolated from pericarp of Citrus sinensis. International Journal of Microbiology and Biotechnology 2010; 5: 22-27.
- Erenler R., Tugba A., Karan T., Elmastas M., Yildiz I., Aksit H., Topcu G., Sanda MA. et al. Chemical constituents isolated from Origanum solymicum with antioxidant activitites. The Eurasia Proceedings of Science Technology Engineering and Mathematics 2017; 1: 139-145.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Structural Biology, Food Engineering, Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLES |
| Authors | |
| Project Number | 1919B012100875 |
| Publication Date | January 22, 2024 |
| Submission Date | May 25, 2023 |
| Acceptance Date | August 28, 2023 |
| Published in Issue | Year 2024 Volume: 7 Issue: 1 |
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