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Obez Sıçanlarda Selenyum ve N-Asetil Sistein’in Fertilite/ İnfertilite ve Karaciğer Üzerine Etkisi

Year 2022, Volume: 9 Issue: 1, 1 - 12, 29.04.2022

Gizem Başaran Alpaslan Gokcimen Nazlı Karagöz Can Uygar Kabaoğlu

https://doi.org/10.47572/muskutd.725703

Abstract

Modern yaşamın getirileri ile beraber değişen beslenme alışkanlıkları ve fiziksel aktivite azlığı, obezitenin dünya genelinde bir halk sağlığı sorunu haline gelmesine neden olmaktadır. Obez bireylerde çeşitli metabolik veya sistemik hastalıklar ortaya çıkmaktadır. Çalışmamızda obeziteye bağlı olarak gelişen karaciğer yağlanması ve fertilite kaybı üzerine selenyum ve N- asetil sistein tedavisinin etkilerini ortaya koymak amaçlanmaktadır. Bu doğrultuda histokimyasal ve immünohistokimyasal tekniklerin yanı sıra serum biyokimya analizleri, oksidatif stres parametreleri ve sperm parametreleri tayinleri yapılmıştır. Selenyum ve N- asetil sisteinin, obezitenin karaciğer ve testis üzerinde oluşturduğu hasarı hem yapısal hem de biyokimyasal düzeyde azalttığını tespit ettik. Ulusal ve uluslararası literatürde ilk kez bu ajanlar kullanılarak obez sıçanlar üzerindeki tedavi edici etkileri gösterilmiştir.  

Keywords

Fertilite Fertilite Karaciğer N-Asetil Sistein Obezite Selenyum

Supporting Institution

Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

TPF-17040

Thanks

Bilimsel Araştırma Koordinasyon Birimi’ ne desteğinden ötürü teşekkür ederiz.

References

  • 1. Li M, Ye T, Wang X-X ve ark. Effect of octreotide on hepatic steatosis in diet-induced obesity in rats. PLoS ONE.2016;11(3):E0152085.
  • 2. Huh JH, Kim KJ, Kim SU ve ark. Obesity is more closely related with hepatic steatosis and fibrosis measured by transient elastography than metabolic health status. Metabol Clin Exper. 2017;66:23-31.
  • 3. Li S, Meng F, Liao X ve ark. Therapeutic role of ursolic acid on ameliorating hepatic steatosis and improving metabolic disorders in high-fat diet-induced non-alcoholic fatty liver disease rats. PLoS ONE. 2014;9(1):e86724.
  • 4. Erdemir F. Obezite ve erkek infertilitesi. J Clin Analytical Med. 2013;4(1):76-82.
  • 5. Özgür B, Eroğlu M. Obezite ve infertilite. Androloji Bült. 2012:254-7.
  • 6. Özata M, Yılmaz İ, Mergen M, Öktenli Ç, Aydın A. Erkek obezitesinde bozulmuş antioksidan kapasite ve hipoçinkonemi. Turk J Endocrinol Metab. 2003;2:47-51.
  • 7. Ersoy O, Köseoğlu H. Karaciğer hastalıkların N-asetil sistein kullanımı. Güncel Gastroenterol. 2014;18(1):119-24.
  • 8. Akşit H, Akşit D, Bildik A ve ark. Deneysel karaciğer intoksikasyonunda N-asetil sistein’in glutatyon metabolizması ve lipid peroksidasyonuna etkileri. Ankara Univ Vet Fak Derg. 2015;62:1-5.
  • 9. Ciftci H, Verit A, Savas M, Yeni E, Erel O. Effects of N-acetylcysteine on semen parameters and oxidative/antioxidant status. Urology. 2009;74(1):73-6.
  • 10. Nazıroğlu M, Çay M. Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin. Biol Trace Elem Res. 2001;79:149-59.
  • 11. Dkhil MA, Zrieq R, Quraish SA ve ark. Moneim selenium nanoparticles attenuate oxidative stress and testicular damage in streptozotocin-induced diabetic rats. Molecules. 2016;21:1517.
  • 12. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968;125(1):180-98.
  • 13. Beutler E, Dubon O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882-8.
  • 14. Kruger TF, Menkveld R, Stander FS ve ark. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril. 1986;46(6):1118-23.
  • 15. Huang H, Yan Z, Chen Y ve ark. A social contagious model of the obesity epidemic. Scientific Reports. 2016;6:37961.
  • 16. Sayin FK, Buyukbas S, Basarali MK ve ark. Effects of silybum marianum extract on high-fat diet induced metabolic disorders in rats. Pol J Food Nutr Sci. 2016;66(1):43-50.
  • 17. Moreno-Fernández S, Garcés-Rimón M, Vera G ve ark. High fat/high glucose diet induces metabolic syndrome in an experimental rat model. Nutrients. 2018;10(10):1502.
  • 18. Liu F, Wang X, Shi H ve ark. Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model. Br J Nutr. 2017;117(9):1332-42.
  • 19. Amri Z, Ghorbel A, Turki M ve ark. Effect of pomegranate extracts on brain antioxidant markers and cholinesterase activity in high fat-high fructose diet induced obesity in rat model. BMC Complement Altern Med. 2017;17(1):339.
  • 20. Van Herck MA, Vonghia L, Francque SM. Animal models of nonalcoholic fatty liver disease-a starter's guide. Nutrients. 2017;9(10):1072.
  • 21. Yao X, Lin Z, Jiang C ve ark. Cyclocarya paliurus prevents high fat diet induced hyperlipidemia and obesity in Sprague–Dawley rats. Can J Physiol Pharmacol. 2015;93(8):677-86.
  • 22. Gurung S, Agbaga M-P, Myers DA. Cognitive differences between Sprague-Dawley rats selectively bred for sensitivity or resistance to diet induced obesity. Behav Brain Res. 2016;311:122-30.
  • 23. Ahmad A, Ali T, Kim MW ve ark. Adiponectin homolog novel osmotin protects obesity/diabetes-induced NAFLD by upregulating Adipors/Pparα signaling in Ob/Ob and Db/Db transgenic mouse models. Metabolism. 2019;90:31-43.
  • 24. Soltys K, Dikdan G, Koneru B. Oxidative sress in fatty livers of obese Zucker rats: Rapid amelioration and improved tolerance to warm ischemia with tocopherol. Hepatology. 2001;34(1):13-8.
  • 25. Niu S, Wang L, He M ve ark. Exendin-4 regulates redox homeostasis in rats fed with high-fat diet. Acta Biochim Biophys Sin (Shanghai). 2015;47(6):397-403.
  • 26. Punhagui APF, Teixeira GR, de Freitas MC ve ark. Intermittent resistance exercise and obesity, considered separately or combined, impair spermatic parameters in adult male Wistar rats. Int J Exp Pathol. 2018;99(2):95-102.
  • 27. Li B, Zhang C, Zhan YT. Nonalcoholic fatty liver disease cirrhosis: A review of its epidemiology, risk factors, clinical presentation, diagnosis, management, and prognosis. Can J Gastroenterol Hepatol. 2018;2018:2784537
  • 28. Miao XL, Gao GM, Jiang L ve ark. Asiatic acid attenuates high-fat diet induced impaired spermatogenesis. Exp Ther Med. 2018;15(3):2397-403.
  • 29. Vendramini V, Cedenho AP, Miraglia SM ve ark. Reproductive function of the male obese Zucker rats: Alteration in sperm production and sperm DNA damage. Reprod Sci. 2014;21(2):221-9.
  • 30. Lu ZH, Mu YM, Wang BA ve ark. Saturated free fatty acids, palmitic acid and stearic acid, induce apoptosis by stimulation of ceramide generation in rat testicular leydig cell. Biochem Biophys Res Commun. 2003;303(4):1002-7.
  • 31. Yan WJ, Mu Y, Yu N ve ark. Protective effects of Metformin on reproductive function in obese male rats induced by high-fat diet. J Assist Reprod Genet. 2015;32(7):1097-104.
  • 32. Hamza RZ, Al-Harbi MS. Monosodium glutamate induced testicular toxicity and the possible ameliorative role of vitamin E or selenium in male rats. Toxicol Rep. 2014;1:1037-45.
  • 33. Amraoui W, Adjabi N, Bououza F ve ark. Modulatory role of selenium and vitamin E, natural antioxidants, against Bisphenol A-induced oxidative stress in Wistar Albinos rats. Toxicol Res. 2018;34(3):231-9.
  • 34. Asadpour R, Shahbazfar AA, Kıanıfard D ve ark. Comparison of the protective effects of garlic (Allium sativum L) extract, vitamin E and N acetyl cystein on testis structure and sperm quality in rats treated with lead acetate. Rev Med Vet (Toulouse). 2013;164(1):27-33.
  • 35. Ozkol H, Bulut G, Balahoroglu R ve ark. Protective effects of selenium, N acetylcysteine and vitamin E against acute ethanol intoxication in rats. Biol Trace Elem Res. 2017;175(1):177-85.
  • 36. Uzun MA, Koksal N, Kadioglu H ve ark. Effects of N-acetylcysteine on regeneration following partial hepatectomy in rats with nonalcoholic fatty liver disease. Surg Today. 2009;39(7):592-7.
  • 37. Titchenell PM, Lazar MA, Birnbaum MJ. Unraveling the regulation of hepatic metabolism by insulin. Trends Endocrinol Metab. 2017;28(7):497-505.
  • 38. Fan Y, Liu Y, Xue K ve ark. Diet-induced obesity in male C57BL/6 mice decreases fertility as a consequence of disrupted blood-testis barrier. PLoS ONE. 2015;10(4):e0120775.
  • 39. Jallouli M, El Bini Dhouib I, Dhouib H ve ark. Disruption of steroidogenesis after dimethoate exposure and efficacy of N-acetylcysteine in rats: an old drug with new approaches. Environ Sci Pollut Res Int. 2016;23(8):7975-84.
  • 40. Dokras A, Playford M, Kris-Etherton PM ve ark. Impact of hormonal contraception and weight loss on high-density lipoprotein cholesterol efflux and lipoprotein particles in women with polycystic ovary syndrome. Clin Endocrinol (Oxf). 2017;86(5):739-46.

Effect of Selenium and N-Acetyl Cysteine on Fertility/ Infertility and Liver in Obese Rats

Year 2022, Volume: 9 Issue: 1, 1 - 12, 29.04.2022

Gizem Başaran Alpaslan Gokcimen Nazlı Karagöz Can Uygar Kabaoğlu

https://doi.org/10.47572/muskutd.725703

Abstract

Changing dietary habits and lack of physical activity, which are the returns of modern life, cause obesity to become a public health problem worldwide. Various metabolic and systemic diseases develop in obese individuals. The aim of this study was to evaluate the effects of selenium and N-acetylcysteine treatment on obesity-related liver adiposity and loss of fertility. For this purpose, histochemical and immunohistochemical techniques, as well as serum biochemistry analyzes, oxidative stress parameters and sperm parameters were determined. We found that selenium and N-acetylcysteine reduce the damage caused by obesity on both liver and testis both structurally and biochemically. In the national and international literature, the therapeutic effects on obese rats were first demonstrated using these agents. 

Keywords

Fertility Liver N-Acetylcysteine Obesity Selenium

Project Number

TPF-17040

References

  • 1. Li M, Ye T, Wang X-X ve ark. Effect of octreotide on hepatic steatosis in diet-induced obesity in rats. PLoS ONE.2016;11(3):E0152085.
  • 2. Huh JH, Kim KJ, Kim SU ve ark. Obesity is more closely related with hepatic steatosis and fibrosis measured by transient elastography than metabolic health status. Metabol Clin Exper. 2017;66:23-31.
  • 3. Li S, Meng F, Liao X ve ark. Therapeutic role of ursolic acid on ameliorating hepatic steatosis and improving metabolic disorders in high-fat diet-induced non-alcoholic fatty liver disease rats. PLoS ONE. 2014;9(1):e86724.
  • 4. Erdemir F. Obezite ve erkek infertilitesi. J Clin Analytical Med. 2013;4(1):76-82.
  • 5. Özgür B, Eroğlu M. Obezite ve infertilite. Androloji Bült. 2012:254-7.
  • 6. Özata M, Yılmaz İ, Mergen M, Öktenli Ç, Aydın A. Erkek obezitesinde bozulmuş antioksidan kapasite ve hipoçinkonemi. Turk J Endocrinol Metab. 2003;2:47-51.
  • 7. Ersoy O, Köseoğlu H. Karaciğer hastalıkların N-asetil sistein kullanımı. Güncel Gastroenterol. 2014;18(1):119-24.
  • 8. Akşit H, Akşit D, Bildik A ve ark. Deneysel karaciğer intoksikasyonunda N-asetil sistein’in glutatyon metabolizması ve lipid peroksidasyonuna etkileri. Ankara Univ Vet Fak Derg. 2015;62:1-5.
  • 9. Ciftci H, Verit A, Savas M, Yeni E, Erel O. Effects of N-acetylcysteine on semen parameters and oxidative/antioxidant status. Urology. 2009;74(1):73-6.
  • 10. Nazıroğlu M, Çay M. Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin. Biol Trace Elem Res. 2001;79:149-59.
  • 11. Dkhil MA, Zrieq R, Quraish SA ve ark. Moneim selenium nanoparticles attenuate oxidative stress and testicular damage in streptozotocin-induced diabetic rats. Molecules. 2016;21:1517.
  • 12. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968;125(1):180-98.
  • 13. Beutler E, Dubon O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882-8.
  • 14. Kruger TF, Menkveld R, Stander FS ve ark. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril. 1986;46(6):1118-23.
  • 15. Huang H, Yan Z, Chen Y ve ark. A social contagious model of the obesity epidemic. Scientific Reports. 2016;6:37961.
  • 16. Sayin FK, Buyukbas S, Basarali MK ve ark. Effects of silybum marianum extract on high-fat diet induced metabolic disorders in rats. Pol J Food Nutr Sci. 2016;66(1):43-50.
  • 17. Moreno-Fernández S, Garcés-Rimón M, Vera G ve ark. High fat/high glucose diet induces metabolic syndrome in an experimental rat model. Nutrients. 2018;10(10):1502.
  • 18. Liu F, Wang X, Shi H ve ark. Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model. Br J Nutr. 2017;117(9):1332-42.
  • 19. Amri Z, Ghorbel A, Turki M ve ark. Effect of pomegranate extracts on brain antioxidant markers and cholinesterase activity in high fat-high fructose diet induced obesity in rat model. BMC Complement Altern Med. 2017;17(1):339.
  • 20. Van Herck MA, Vonghia L, Francque SM. Animal models of nonalcoholic fatty liver disease-a starter's guide. Nutrients. 2017;9(10):1072.
  • 21. Yao X, Lin Z, Jiang C ve ark. Cyclocarya paliurus prevents high fat diet induced hyperlipidemia and obesity in Sprague–Dawley rats. Can J Physiol Pharmacol. 2015;93(8):677-86.
  • 22. Gurung S, Agbaga M-P, Myers DA. Cognitive differences between Sprague-Dawley rats selectively bred for sensitivity or resistance to diet induced obesity. Behav Brain Res. 2016;311:122-30.
  • 23. Ahmad A, Ali T, Kim MW ve ark. Adiponectin homolog novel osmotin protects obesity/diabetes-induced NAFLD by upregulating Adipors/Pparα signaling in Ob/Ob and Db/Db transgenic mouse models. Metabolism. 2019;90:31-43.
  • 24. Soltys K, Dikdan G, Koneru B. Oxidative sress in fatty livers of obese Zucker rats: Rapid amelioration and improved tolerance to warm ischemia with tocopherol. Hepatology. 2001;34(1):13-8.
  • 25. Niu S, Wang L, He M ve ark. Exendin-4 regulates redox homeostasis in rats fed with high-fat diet. Acta Biochim Biophys Sin (Shanghai). 2015;47(6):397-403.
  • 26. Punhagui APF, Teixeira GR, de Freitas MC ve ark. Intermittent resistance exercise and obesity, considered separately or combined, impair spermatic parameters in adult male Wistar rats. Int J Exp Pathol. 2018;99(2):95-102.
  • 27. Li B, Zhang C, Zhan YT. Nonalcoholic fatty liver disease cirrhosis: A review of its epidemiology, risk factors, clinical presentation, diagnosis, management, and prognosis. Can J Gastroenterol Hepatol. 2018;2018:2784537
  • 28. Miao XL, Gao GM, Jiang L ve ark. Asiatic acid attenuates high-fat diet induced impaired spermatogenesis. Exp Ther Med. 2018;15(3):2397-403.
  • 29. Vendramini V, Cedenho AP, Miraglia SM ve ark. Reproductive function of the male obese Zucker rats: Alteration in sperm production and sperm DNA damage. Reprod Sci. 2014;21(2):221-9.
  • 30. Lu ZH, Mu YM, Wang BA ve ark. Saturated free fatty acids, palmitic acid and stearic acid, induce apoptosis by stimulation of ceramide generation in rat testicular leydig cell. Biochem Biophys Res Commun. 2003;303(4):1002-7.
  • 31. Yan WJ, Mu Y, Yu N ve ark. Protective effects of Metformin on reproductive function in obese male rats induced by high-fat diet. J Assist Reprod Genet. 2015;32(7):1097-104.
  • 32. Hamza RZ, Al-Harbi MS. Monosodium glutamate induced testicular toxicity and the possible ameliorative role of vitamin E or selenium in male rats. Toxicol Rep. 2014;1:1037-45.
  • 33. Amraoui W, Adjabi N, Bououza F ve ark. Modulatory role of selenium and vitamin E, natural antioxidants, against Bisphenol A-induced oxidative stress in Wistar Albinos rats. Toxicol Res. 2018;34(3):231-9.
  • 34. Asadpour R, Shahbazfar AA, Kıanıfard D ve ark. Comparison of the protective effects of garlic (Allium sativum L) extract, vitamin E and N acetyl cystein on testis structure and sperm quality in rats treated with lead acetate. Rev Med Vet (Toulouse). 2013;164(1):27-33.
  • 35. Ozkol H, Bulut G, Balahoroglu R ve ark. Protective effects of selenium, N acetylcysteine and vitamin E against acute ethanol intoxication in rats. Biol Trace Elem Res. 2017;175(1):177-85.
  • 36. Uzun MA, Koksal N, Kadioglu H ve ark. Effects of N-acetylcysteine on regeneration following partial hepatectomy in rats with nonalcoholic fatty liver disease. Surg Today. 2009;39(7):592-7.
  • 37. Titchenell PM, Lazar MA, Birnbaum MJ. Unraveling the regulation of hepatic metabolism by insulin. Trends Endocrinol Metab. 2017;28(7):497-505.
  • 38. Fan Y, Liu Y, Xue K ve ark. Diet-induced obesity in male C57BL/6 mice decreases fertility as a consequence of disrupted blood-testis barrier. PLoS ONE. 2015;10(4):e0120775.
  • 39. Jallouli M, El Bini Dhouib I, Dhouib H ve ark. Disruption of steroidogenesis after dimethoate exposure and efficacy of N-acetylcysteine in rats: an old drug with new approaches. Environ Sci Pollut Res Int. 2016;23(8):7975-84.
  • 40. Dokras A, Playford M, Kris-Etherton PM ve ark. Impact of hormonal contraception and weight loss on high-density lipoprotein cholesterol efflux and lipoprotein particles in women with polycystic ovary syndrome. Clin Endocrinol (Oxf). 2017;86(5):739-46.
There are 40 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences, ​Internal Diseases
Journal Section Original Article
Authors

Gizem Başaran

Alpaslan Gokcimen

Nazlı Karagöz Can 0000-0003-2023-8246

Uygar Kabaoğlu

Project Number TPF-17040
Publication Date April 29, 2022
Submission Date April 23, 2020
Published in Issue Year 2022 Volume: 9 Issue: 1

Cite

APA Başaran, G., Gokcimen, A., Karagöz Can, N., Kabaoğlu, U. (2022). Obez Sıçanlarda Selenyum ve N-Asetil Sistein’in Fertilite/ İnfertilite ve Karaciğer Üzerine Etkisi. Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi, 9(1), 1-12. https://doi.org/10.47572/muskutd.725703
AMA Başaran G, Gokcimen A, Karagöz Can N, Kabaoğlu U. Obez Sıçanlarda Selenyum ve N-Asetil Sistein’in Fertilite/ İnfertilite ve Karaciğer Üzerine Etkisi. MMJ. April 2022;9(1):1-12. doi:10.47572/muskutd.725703
Chicago Başaran, Gizem, Alpaslan Gokcimen, Nazlı Karagöz Can, and Uygar Kabaoğlu. “Obez Sıçanlarda Selenyum Ve N-Asetil Sistein’in Fertilite/ İnfertilite Ve Karaciğer Üzerine Etkisi”. Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi 9, no. 1 (April 2022): 1-12. https://doi.org/10.47572/muskutd.725703.
EndNote Başaran G, Gokcimen A, Karagöz Can N, Kabaoğlu U (April 1, 2022) Obez Sıçanlarda Selenyum ve N-Asetil Sistein’in Fertilite/ İnfertilite ve Karaciğer Üzerine Etkisi. Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi 9 1 1–12.
IEEE G. Başaran, A. Gokcimen, N. Karagöz Can, and U. Kabaoğlu, “Obez Sıçanlarda Selenyum ve N-Asetil Sistein’in Fertilite/ İnfertilite ve Karaciğer Üzerine Etkisi”, MMJ, vol. 9, no. 1, pp. 1–12, 2022, doi: 10.47572/muskutd.725703.
ISNAD Başaran, Gizem et al. “Obez Sıçanlarda Selenyum Ve N-Asetil Sistein’in Fertilite/ İnfertilite Ve Karaciğer Üzerine Etkisi”. Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi 9/1 (April 2022), 1-12. https://doi.org/10.47572/muskutd.725703.
JAMA Başaran G, Gokcimen A, Karagöz Can N, Kabaoğlu U. Obez Sıçanlarda Selenyum ve N-Asetil Sistein’in Fertilite/ İnfertilite ve Karaciğer Üzerine Etkisi. MMJ. 2022;9:1–12.
MLA Başaran, Gizem et al. “Obez Sıçanlarda Selenyum Ve N-Asetil Sistein’in Fertilite/ İnfertilite Ve Karaciğer Üzerine Etkisi”. Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi, vol. 9, no. 1, 2022, pp. 1-12, doi:10.47572/muskutd.725703.
Vancouver Başaran G, Gokcimen A, Karagöz Can N, Kabaoğlu U. Obez Sıçanlarda Selenyum ve N-Asetil Sistein’in Fertilite/ İnfertilite ve Karaciğer Üzerine Etkisi. MMJ. 2022;9(1):1-12.
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