Research Article
Effect of different protein sources on protease activity of Neon tetra, Paracheirodon innesi (Myers, 1936) juveniles
Abstract
Neon tetra, Paracheirodon innesi, is a highly valuable fish species for the aquarium sector. In this study, to determine the inhibitory effects of different protein sources (soybean protein concentrate, soybean meal, fish meal, shrimp meal, krill meal, corn gluten and chicken meal) on early life stage proteases, invitro assays were applied. At the end of the study, the highest inhibition ratios were obtained from soybean protein concentrate (53.59±0.58 %) as plant protein sources. On the other hand, the minimum inhibition ratios were obtained from shrimp and chicken meal (21.88±0.74 % and 21.88±1.26 %, respectively) (p>0.05). In conclusion, it is concluded that the soy bean protein concentrate should not be used in the commercial diets as protein source on ongrowing size of this species.
References
- Alarcon, F. J., Moyano, F. J., Diaz, M., Fernandez-Diaz, C. & Yufera, M. (1999). Optimization of the protein fraction of microcapsules used in feeding of marine fish larvae using in vitro digestibility techniques. Aquaculture Nutrition, 5: 107-113.
- Alptekin, C. & Gökçek, K. (2016). Balık yemlerinde kullanılan farklı protein kaynaklarının Sibirya mersini, Acipencer baerii Brandt 1869, juvenillerinin proteaz aktivitesi üzerine etkileri. Yunus Araştırma Bülteni, 3: 201-207. https://doi.org/10.17693/yunus.32866
- Budiardi, T., Solehudin dan, M. A. & Wahjuningrum, D. (2008). Production of M-Size Neon tetra fish Paracheriodon innesi in recirculation system with density of 25, 50, 75 and 100 litre-1. Journal Akuakultur Indonesia, 7(1): 19-24.
- Clothier, J. & Lythgoe, J. N. (1987). Light-induced colour changes by the iridophores of the Neon tetra, Paracheirodon innesi. Journal of Cell Science, (88): 663-668.
- Garcia-Carreno, F. L. (1996). Proteinase inhibitors. Trends in Food Science & Technology, 7(6): 197-204. https://doi.org/10.1016/0924-2244(96)10023-6
- Gökçek, K., Szabo, T., Alptekin, C., Kurt, R., Töre, Y. & Urbanyi, B. (2016). A preliminary study on protease activity of Russian sturgeon, Acipencer gueldenstaedtii Brandt and Ratzenburg, 1833, at early life stages. Turkish Journal of Fisheries and Aquatic Sciences, 16(4): 1025-1029. http://doi.org/10.4194/1303-2712-v16_4_29
- Gökçek, K., Szabo, T., Urbanyi, B., Erbatur, İ., Sevgili, H. & Kanyılmaz, ,M. (2018). Effect of different protein sources on protease activity of European catfish, Silurus glanis Linneaus 1758, juvenile. FABA 2018. Ankara, Turkey. Halver, J. E. (2002). Fish Nutrition. Academic Press. p. 506.
- Kucharczyk, D., Targońska, K., Żarski, D., Krejszeff, S., Kupren. K., Łuczyński, M. J. & Szczerbowski, A. (2010). The reproduction of Neon tetra, Paracheirodon innesi (Myers, 1936), under controlled conditions. Polısh Journal of Natural Scıences, 25(1): 81–92. https://doi.org/10.2478/v10020-010-0006-x
- Kurt, R. & Gökçek, K. (2018). Farklı protein kaynaklarının Çuka Mersini, Acipenser ruthenus Linnaeus 1758, juvenillerinde proteaz aktivitesi üzerine etkileri. Journal of Limnology and Freshwater Fisheries Research, 4(1): 1-5. https://doi.org/10.17216/limnofish.325726
- Mayoza, L., Agus, A., Lili, W. & Rizal, A. (2018). Analytical business development on Neon tetra (Paracheirodon innesi) ornamental fish cultivation. Global Scientific Journals, 6(7): 938-945.
- Sanaye, S. V., Dhaker, H. S., Tibile, R. M. & Mhatre, V. D. (2014). Effect of green water and mixed zooplankton on growth and survival in Neon tetra, Paracheirodon innesi (Myers, 1936) during larval and early fry rearing. World Academy of Science, Engineering and Technology International Journal of Bioengineering and Life Sciences, 8(2): 159-163.
- Sealey, W. M., Barrows, F. T., Casten, M. & Hardy, R.W. (2009). Dietary Protein Source and Level Affects Growth in Neon Tetras. North American Journal of Aquaculture, 71:320–324.
- Töre, Y., Gökçek, K., Alptekin, C., Szabo, T. & Urbanyi, B. (2014). Turna, Esox lucius Linneaus 1758, balığı larvalarının proteaz aktivitesi üzerine ticari yem hammaddelerinin inhibisyon etkilerinin tespiti. Doğu Anadolu 5. Su Ürünleri Sempozyumu Bildiri Kitabı, pp. 228-229.
- Yoshioka, S., Matsuhana, B., Tanaka, S., Inouye, Y., Oshima, N. & Kinoshita, S. (2011). Mechanism of variable structural colour in the neon tetra: quantitative evaluation of the Venetian blind model. Journal of the Royal Society Interface, 8(54): 56–66. https://doi.org/10.1098/rsif.2010.0253
- Walter, H. E. (1984). Proteinases: methods with hemoglobin, casein and azocoll as sub-strates. In: Methods of Enzymatic Analysis (Bergmeyer, H.J. ed.), 5: 270-277.
Farklı protein kaynaklarının Neon tetra, Paracheirodon innesi (Myers, 1936) jüvenillerinin proteaz aktivitesi üzerine etkisi
Abstract
Neon tetra, Paracheirodon innesi, akvaryum sektörü açısından oldukça yüksek değere sahip bir balık türüdür. Bu çalışmada, farklı protein kaynaklarının (soya protein konsantresi, soya unu, balık unu, karides unu, krill unu, mısır glüteni, ve tavuk unu) erken dönem proteaz enzimleri üzerine inhibe edici etkilerini bulmak amacıyla invitro analizler yapılmıştır. Çalışma sonunda, en yüksek inhibisyon oranı bitkisel protein kaynaklarından soya protein konsantresinden (%53,59±0,58) elde edilmiştir. Diğer yandan, en düşük inhibisyon oranları karides ve tavuk unundan elde edilmiştir (sırasıyla, %21,88±0,74 ve %21,88±1,26) (p>0,05). Sonuç olarak, bu türün erken dönem beslenmesinde karma yemlerinde soya protein konsanteresinin protein kaynağı olarak kullanılmaması gerektiği kanaati doğmuştur.
References
- Alarcon, F. J., Moyano, F. J., Diaz, M., Fernandez-Diaz, C. & Yufera, M. (1999). Optimization of the protein fraction of microcapsules used in feeding of marine fish larvae using in vitro digestibility techniques. Aquaculture Nutrition, 5: 107-113.
- Alptekin, C. & Gökçek, K. (2016). Balık yemlerinde kullanılan farklı protein kaynaklarının Sibirya mersini, Acipencer baerii Brandt 1869, juvenillerinin proteaz aktivitesi üzerine etkileri. Yunus Araştırma Bülteni, 3: 201-207. https://doi.org/10.17693/yunus.32866
- Budiardi, T., Solehudin dan, M. A. & Wahjuningrum, D. (2008). Production of M-Size Neon tetra fish Paracheriodon innesi in recirculation system with density of 25, 50, 75 and 100 litre-1. Journal Akuakultur Indonesia, 7(1): 19-24.
- Clothier, J. & Lythgoe, J. N. (1987). Light-induced colour changes by the iridophores of the Neon tetra, Paracheirodon innesi. Journal of Cell Science, (88): 663-668.
- Garcia-Carreno, F. L. (1996). Proteinase inhibitors. Trends in Food Science & Technology, 7(6): 197-204. https://doi.org/10.1016/0924-2244(96)10023-6
- Gökçek, K., Szabo, T., Alptekin, C., Kurt, R., Töre, Y. & Urbanyi, B. (2016). A preliminary study on protease activity of Russian sturgeon, Acipencer gueldenstaedtii Brandt and Ratzenburg, 1833, at early life stages. Turkish Journal of Fisheries and Aquatic Sciences, 16(4): 1025-1029. http://doi.org/10.4194/1303-2712-v16_4_29
- Gökçek, K., Szabo, T., Urbanyi, B., Erbatur, İ., Sevgili, H. & Kanyılmaz, ,M. (2018). Effect of different protein sources on protease activity of European catfish, Silurus glanis Linneaus 1758, juvenile. FABA 2018. Ankara, Turkey. Halver, J. E. (2002). Fish Nutrition. Academic Press. p. 506.
- Kucharczyk, D., Targońska, K., Żarski, D., Krejszeff, S., Kupren. K., Łuczyński, M. J. & Szczerbowski, A. (2010). The reproduction of Neon tetra, Paracheirodon innesi (Myers, 1936), under controlled conditions. Polısh Journal of Natural Scıences, 25(1): 81–92. https://doi.org/10.2478/v10020-010-0006-x
- Kurt, R. & Gökçek, K. (2018). Farklı protein kaynaklarının Çuka Mersini, Acipenser ruthenus Linnaeus 1758, juvenillerinde proteaz aktivitesi üzerine etkileri. Journal of Limnology and Freshwater Fisheries Research, 4(1): 1-5. https://doi.org/10.17216/limnofish.325726
- Mayoza, L., Agus, A., Lili, W. & Rizal, A. (2018). Analytical business development on Neon tetra (Paracheirodon innesi) ornamental fish cultivation. Global Scientific Journals, 6(7): 938-945.
- Sanaye, S. V., Dhaker, H. S., Tibile, R. M. & Mhatre, V. D. (2014). Effect of green water and mixed zooplankton on growth and survival in Neon tetra, Paracheirodon innesi (Myers, 1936) during larval and early fry rearing. World Academy of Science, Engineering and Technology International Journal of Bioengineering and Life Sciences, 8(2): 159-163.
- Sealey, W. M., Barrows, F. T., Casten, M. & Hardy, R.W. (2009). Dietary Protein Source and Level Affects Growth in Neon Tetras. North American Journal of Aquaculture, 71:320–324.
- Töre, Y., Gökçek, K., Alptekin, C., Szabo, T. & Urbanyi, B. (2014). Turna, Esox lucius Linneaus 1758, balığı larvalarının proteaz aktivitesi üzerine ticari yem hammaddelerinin inhibisyon etkilerinin tespiti. Doğu Anadolu 5. Su Ürünleri Sempozyumu Bildiri Kitabı, pp. 228-229.
- Yoshioka, S., Matsuhana, B., Tanaka, S., Inouye, Y., Oshima, N. & Kinoshita, S. (2011). Mechanism of variable structural colour in the neon tetra: quantitative evaluation of the Venetian blind model. Journal of the Royal Society Interface, 8(54): 56–66. https://doi.org/10.1098/rsif.2010.0253
- Walter, H. E. (1984). Proteinases: methods with hemoglobin, casein and azocoll as sub-strates. In: Methods of Enzymatic Analysis (Bergmeyer, H.J. ed.), 5: 270-277.
There are 15 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Zoology |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | June 30, 2022 |
| Submission Date | February 10, 2022 |
| Acceptance Date | March 4, 2022 |
| Published in Issue | Year 2022 Volume: 4 Issue: 1 |
