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Erişkin ratlarda Zerdaçal (Curcuma longa L.) Takviyesinin Kalp Dokusundaki Oksidan/Antioksidan Etkilerinin İncelenmesi

Year 2023, Volume: 11 Issue: 1, 183 - 188, 30.04.2023
https://doi.org/10.24998/maeusabed.1265094

Abstract

Bu çalışmada, erişkin ratlarda zerdaçal (kurkumin) takviyesinin kalp dokusundaki oksidatif stres ve antioksidan savunma sistemi üzerinde meydana getirdiği etkileri incelendi. Çalışmada 8 haftalık 16 adet rat kullanılmış ve bu ratlar iki gruba ayrılmıştır. İlk grup olan deney grubundaki ratlara (n=8) 28 gün süresince günde 300 mg/kg dozunda kurkumin (C1386; Sigma Chemical, St. Louis, MO) mısır yağı içinde çözdürülerek ağızdan gavaj yöntemi ile verildi. İkinci grup olan kontrol grubundaki ratlara ise portör etkisini yok etmek için kurkumin ile eşit miktarda mısır yağı verildi. Ötenazi gerçekleştirildikten sonra kalp dokuları alınarak total antioksidan kapasiteleri (TAS), total oksidan kapasiteleri (TOS), malondialdehit (MDA) ve glutatyon (GSH) seviyeleri analiz edildi. Kurkumin takviyesi GSH seviyelerini önemli ölçüde artırmıştır (P<0,05). Bununla birlikte, kurkumin takviyesi istatistiksel olarak anlamlı olamamasına rağmen MDA seviyelerini azaltmıştır (P>0,05). Total antioksidan kapasitesi (TAS) ve total oksidan kapasitesi (TOS) oranları istatistiksel olarak anlamlı bulundu. Kurkumin takviyeli diğer grupta MDA seviyeleri kontrol grubuna oranla azalma eğilimi göstermiş olup iki grup arasında anlamlı istatistiksel bir fark bulunmamıştır. Kurkumin takviyesi erişkin ratlarda kalp dokusunu oksidatif hasara karşı korumakta ve antioksidan savunma sistemini güçlendirmektedir.

References

  • Choudhuri, T., Pal, S., Agwarwal, ML., Das, T., Sa, G., 2002. Curcumin induces apoptosis in human breast cancer cells through p53-dependent Bax induction. FEBS Lett; 512(1-3):334-40.
  • Dkhar, P., Sharma, R., 2010. Effect of dimethylsulphoxide and curcumin on protein carbonyls and reactive oxygen species of cerebral hemispheres of mice as a function of age. Int J Dev Neurosci. 28:351-7.
  • Duan, W., Yang, Y., Yan, J., Yu, S., Liu, J., Zhou, J., et al., 2012. The effects of curcumin post-treatment against myocardial ischemia and reperfusion by activation of the JAK2/STAT3 signaling pathway. Basic Res Cardiol. 107:263.
  • Jenkins, RR., 1993. Exercise, oxidative stress, and antioxidants: A review. Int J Sport Nutr. 3:356-75.
  • Kakarla, P., Vadluri, G., Reddy, KS., 2005. Leeuwenburgh C. Vulnerability of the mid aged rat myocardium to the age- induced oxidative stress: influence of exercise training on antioxidant defense system. Free Radic Res. 39:1211-7.
  • Lowry, OH., Rosebrough, NJ., Farr, AL., Randall, RJ., 1951. Protein measurement with the Folin phenol reagent. J Biol Chem. 193:265-75.
  • Miriyala, S., 2007. Panchatcharam M, Rengarajulu P. Cardioprotective effects of curcumin. Adv Exp Med Biol. 595:359-77.
  • Morimoto, T., Sunagawa, Y., Kawamura, T., Takaya, T., Wada, H., Nagasawa, A., et al., 2008. The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats. J Clin Invest. 118:868-78.
  • Naik, SR., Thakare, VN., Patil, SR., 2011. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats: evidence of its antioxidant property. Exp Toxicol Pathol. 63:419-31.
  • Nakao, C., Ookawara, T., Kizaki, T., Oh-Ishi, S., Miyazaki, H., Haga, S., et al., 2000. Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues. J Appl Physiol. 88:649-54.
  • Nazam Ansari, M., Bhandari, U., Pillai, KK.,2007. Protective role of curcumin in myocardial oxidative damage induced by isoproterenol in rats. Hum Exp Toxicol. 26:933-8.
  • Nirmala, C., Anand, S., Puvanakrishnan, R.,1999. Curcumin treatment modulates collagen metabolism in isoproterenol induced myocardial necrosis in rats. Mol Cell Biochem. 197:31- 7.
  • Parildar, H., Dogru-Abbasoglu, S., Mehmetçik, G., Ozdemirler, G., Koçak-Toker, N., Uysal, M.,2008. Lipid peroxidation potential and antioxidants in the heart tissue of beta-alanine- or taurine- treated old rats. J Nutr Sci Vitaminol (Tokyo). 54:61-5.
  • Reddy, AC., Lokesh, BR., 1994. Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron. Mol Cell Biochem. 137:1-8.
  • Sreejayan, Rao MN., 1997. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol. 49:105-7. Srivastava, G., Mehta, JL., 2009. Currying the heart: curcumin and cardioprotection. J Cardiovasc Pharmacol Ther. 14:22-7.
  • Thiyagarajan, M., Sharma, SS., 2004. Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats. Life Sci. 74:969-85.
  • Venkatesan, N., 1998. Curcumin attenuation of acute adriamycin myocardial toxicity in rats. Br J Pharmacol. 124:425-7.
  • Wongcharoen, W., Phrommintikul, A., 2009. The protective role of curcumin in cardiovascular diseases. Int J Cardiol. 133:145-51.
  • Yeh, CH., Chen, TP., Wu, YC., Lin, YM., 2005. Jing Lin P. Inhibition of NFkappaB activation with curcumin attenuates plasma inflammatory cytokines surge and cardiomyocytic apoptosis following cardiac ischemia/reperfusion. J Surg Res. 125:109-16.
  • Zhao, J., Zhao, Y., Zheng, W., Lu ,Y., Feng, G., Yu, S., 2008. Neuroprotective effect of curcumin on transient focal cerebral ischemia in rats. Brain Res. 1229:224-32.

Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats

Year 2023, Volume: 11 Issue: 1, 183 - 188, 30.04.2023
https://doi.org/10.24998/maeusabed.1265094

Abstract

n present study, the effects of curcumin supplementation on oxidative stress and antioxidant defense system in heart tissue in adult rats were investigated. Sixteen rats (8-weeks-old) were selected and divided into two groups. The first group is the experimental group and these rats (n=8) were gavaged with curcumin, dissolved in corn oil, at a dose of 300 mg/kg per day for 28 days. The second group is the control group, rats in this group were given curcumin and equal amount of corn oil to eliminate the porter effect. Euthanasia was performed and total antioxidant capacity (TAS), total oxidant capacity (TOS), malondialdehyde (MDA) and glutathione (GSH) levels were analyzed from heart tissues. Curcumin supplementation resulted in significantly increased GSH levels(P<0,05). However, curcumin supplementation decreased MDA levels although it was not statistically significant (P>0.05). The total antioxidant capacity (TAS) and total oxidant capacity (TOS) ratios were found to be statistically significant. In the other group supplemented with curcumin, MDA levels tended to decrease compared to the control group, and there was no statistically significant difference between the two groups. Curcumin supplementation protects the heart tissue against oxidative damage and strengthens the antioxidant defense system in adult rats.

References

  • Choudhuri, T., Pal, S., Agwarwal, ML., Das, T., Sa, G., 2002. Curcumin induces apoptosis in human breast cancer cells through p53-dependent Bax induction. FEBS Lett; 512(1-3):334-40.
  • Dkhar, P., Sharma, R., 2010. Effect of dimethylsulphoxide and curcumin on protein carbonyls and reactive oxygen species of cerebral hemispheres of mice as a function of age. Int J Dev Neurosci. 28:351-7.
  • Duan, W., Yang, Y., Yan, J., Yu, S., Liu, J., Zhou, J., et al., 2012. The effects of curcumin post-treatment against myocardial ischemia and reperfusion by activation of the JAK2/STAT3 signaling pathway. Basic Res Cardiol. 107:263.
  • Jenkins, RR., 1993. Exercise, oxidative stress, and antioxidants: A review. Int J Sport Nutr. 3:356-75.
  • Kakarla, P., Vadluri, G., Reddy, KS., 2005. Leeuwenburgh C. Vulnerability of the mid aged rat myocardium to the age- induced oxidative stress: influence of exercise training on antioxidant defense system. Free Radic Res. 39:1211-7.
  • Lowry, OH., Rosebrough, NJ., Farr, AL., Randall, RJ., 1951. Protein measurement with the Folin phenol reagent. J Biol Chem. 193:265-75.
  • Miriyala, S., 2007. Panchatcharam M, Rengarajulu P. Cardioprotective effects of curcumin. Adv Exp Med Biol. 595:359-77.
  • Morimoto, T., Sunagawa, Y., Kawamura, T., Takaya, T., Wada, H., Nagasawa, A., et al., 2008. The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats. J Clin Invest. 118:868-78.
  • Naik, SR., Thakare, VN., Patil, SR., 2011. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats: evidence of its antioxidant property. Exp Toxicol Pathol. 63:419-31.
  • Nakao, C., Ookawara, T., Kizaki, T., Oh-Ishi, S., Miyazaki, H., Haga, S., et al., 2000. Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues. J Appl Physiol. 88:649-54.
  • Nazam Ansari, M., Bhandari, U., Pillai, KK.,2007. Protective role of curcumin in myocardial oxidative damage induced by isoproterenol in rats. Hum Exp Toxicol. 26:933-8.
  • Nirmala, C., Anand, S., Puvanakrishnan, R.,1999. Curcumin treatment modulates collagen metabolism in isoproterenol induced myocardial necrosis in rats. Mol Cell Biochem. 197:31- 7.
  • Parildar, H., Dogru-Abbasoglu, S., Mehmetçik, G., Ozdemirler, G., Koçak-Toker, N., Uysal, M.,2008. Lipid peroxidation potential and antioxidants in the heart tissue of beta-alanine- or taurine- treated old rats. J Nutr Sci Vitaminol (Tokyo). 54:61-5.
  • Reddy, AC., Lokesh, BR., 1994. Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron. Mol Cell Biochem. 137:1-8.
  • Sreejayan, Rao MN., 1997. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol. 49:105-7. Srivastava, G., Mehta, JL., 2009. Currying the heart: curcumin and cardioprotection. J Cardiovasc Pharmacol Ther. 14:22-7.
  • Thiyagarajan, M., Sharma, SS., 2004. Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats. Life Sci. 74:969-85.
  • Venkatesan, N., 1998. Curcumin attenuation of acute adriamycin myocardial toxicity in rats. Br J Pharmacol. 124:425-7.
  • Wongcharoen, W., Phrommintikul, A., 2009. The protective role of curcumin in cardiovascular diseases. Int J Cardiol. 133:145-51.
  • Yeh, CH., Chen, TP., Wu, YC., Lin, YM., 2005. Jing Lin P. Inhibition of NFkappaB activation with curcumin attenuates plasma inflammatory cytokines surge and cardiomyocytic apoptosis following cardiac ischemia/reperfusion. J Surg Res. 125:109-16.
  • Zhao, J., Zhao, Y., Zheng, W., Lu ,Y., Feng, G., Yu, S., 2008. Neuroprotective effect of curcumin on transient focal cerebral ischemia in rats. Brain Res. 1229:224-32.
There are 20 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Şevkinaz Doğan 0000-0001-6180-7586

Aslıhan Cesur Turgut 0000-0002-5824-971X

Publication Date April 30, 2023
Submission Date March 14, 2023
Published in Issue Year 2023 Volume: 11 Issue: 1

Cite

APA Doğan, Ş., & Cesur Turgut, A. (2023). Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 11(1), 183-188. https://doi.org/10.24998/maeusabed.1265094
AMA Doğan Ş, Cesur Turgut A. Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats. Mehmet Akif Ersoy University Journal of Health Sciences Institute. April 2023;11(1):183-188. doi:10.24998/maeusabed.1265094
Chicago Doğan, Şevkinaz, and Aslıhan Cesur Turgut. “Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma Longa L.) Supplementation on Heart Tissue in Adult Rats”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 11, no. 1 (April 2023): 183-88. https://doi.org/10.24998/maeusabed.1265094.
EndNote Doğan Ş, Cesur Turgut A (April 1, 2023) Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats. Mehmet Akif Ersoy University Journal of Health Sciences Institute 11 1 183–188.
IEEE Ş. Doğan and A. Cesur Turgut, “Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 11, no. 1, pp. 183–188, 2023, doi: 10.24998/maeusabed.1265094.
ISNAD Doğan, Şevkinaz - Cesur Turgut, Aslıhan. “Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma Longa L.) Supplementation on Heart Tissue in Adult Rats”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 11/1 (April 2023), 183-188. https://doi.org/10.24998/maeusabed.1265094.
JAMA Doğan Ş, Cesur Turgut A. Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2023;11:183–188.
MLA Doğan, Şevkinaz and Aslıhan Cesur Turgut. “Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma Longa L.) Supplementation on Heart Tissue in Adult Rats”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 11, no. 1, 2023, pp. 183-8, doi:10.24998/maeusabed.1265094.
Vancouver Doğan Ş, Cesur Turgut A. Investigation of Oxidative/Antioxidant Effects of Turmeric (Curcuma longa L.) Supplementation on Heart Tissue in Adult Rats. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2023;11(1):183-8.