Research Article
Pülümür Akarsuyu’ndaki Capoeta umbla’nın Beyin Dokusunda Bir Biyobelirteç Olarak Asetilkolinesteraz Aktivitesinin Mevsimsel Değişimi
Abstract
Bu çalışma, Pülümür
akarsuyundan (Tunceli) alınan Capoeta
umbla (Heckel, 1843)’nın beyin dokusundaki asetilkolinesteraz (AChE) enzim
aktivitesinin mevsimsel değişimini belirlemek amacıyla Mart 2015 ile Şubat 2016
tarihleri arasında gerçekleştirildi. Pülümür Akarsuyu’nun 39̊ 06' 19''N - 39̊ 36' 56''E (A) ve 39̊' 11' 36'N - 39̊ 41' 36''E (B) koordinatları
arasında yakalanan C.umbla' nın AChE
aktivitesindeki mevsimsel dalgalanmalar bir yıl boyunca incelendi. Enzim
aktivitesi, ilkbahar ve yaz aylarında sonbahar ve kışa kıyasla daha yüksekti.
Ayrıca, AChE aktivitesine ait minimum değer kışın, maksimum değer ise
ilkbaharda tespit edildi.
References
- Almeida JR, Gravato C, Guilhermino L. 2015. Effects of temperature in juvenile seabass (Dicentrarchus labrax L.) biomarker responses and behaviour: implications for environmental monitoring. Estuaries and Coasts. 38(1):45-55. doi: 10.1007/s12237-014-9792-7
- Barda I, Purina I, Rimsa E, Balode M. 2014. Seasonal dynamics of biomarkers in infaunal clam Macoma balthica from the Gulf of Riga (Baltic Sea). J Marine Syst. 129(2014): 150-156. doi: 10.1016/j.jmarsys.2013.05.006
- Bocchetti R, Regoli F. 2006. Seasonal variability of oxidative biomarkers, lysosomal parameters, metallothioneins and peroxisomal enzymes in the Mediterranean mussel Mytilus galloprovincialis from Adriatic Sea. Chemosphere. 65(6): 913–921. doi: 10.1016/j.chemosphere.2006.03.049
- Bocquené G, Carbamates Galgani F. 1998. Biological effects of contaminants: cholinesterase inhibition by organophosphate and carbamate compounds (No. 22). Copenhagen, Denmark: International Council for the Exploration of the Sea 19p
- Bueno-Krawcayk ACD, Guiloski IC, Piancini LDS, Azevedo JC, Ramsdorf WA, Ide AH, Guimarães ATB, Cestari MM. Silva de Assis HC. 2015. Multibiomarker in fish to evaluate a river used to water public supply. Chemosphere. 135(2015): 257-264. doi: 10.1016/j.chemosphere.2015.04.064
- Chuiko GM, Kozlovskaya VI. 1989. Seasonal fluctuations of brain acetylcholinesterase activity in perch (Perca fluviatilis L.). Physiology and Toxicology of Hydrobionts, Yaroslavl State University, Yaroslavl. p. 27-37.
- Chuiko GM, Zhelnin Y, PoD'Gornaya VA. 1997. Seasonal fluctuations in brain acetylcholinesterase activity and soluble protein content in roach (Rutilus rutilus L.): a freshwater fish from northwest Russia. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology. 117(3): 251-257. doi: 10.1016/S0742-8413(97)00068-6
- Cikcikoglu Yildirim N, Yildirim N, Danabas D, Danabas S. 2014. Use of acetylcholinesterase, glutathione S-transferase and cytochrome P450 1A1 in Capoeta umbla as biomarkers for monitoring of pollution in Uzuncayir Dam Lake (Tunceli, Turkey). Environmental toxicology and pharmacology. 37(3): 1169-1176. doi: 10.1016/j.etap.2014.04.001
- Ellman GL, Courtney KD, Andres Jr V, Featherstone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology. 7(2): 88-95. doi: 10.1016/0006-2952(61)90145-9
- Forget J, Beliaeff B, Bocquene G. 2003. Acetylcholinesterase activity in copepods (Tigriopus brevicornis) from the Vilaine River estuary, France, as a biomarker of neurotoxic contaminants. Aquatic Toxicology. 62(3): 195-204. doi: 10.1016/S0166-445X(02)00084-X
- Fulton MH, Key PB. 2001. Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environmental Toxicology and Chemistry. 20(1): 37-45. doi: 10.1002/etc.5620200104
- Gadzala-Kopciuch R, Berecka B, Bartoszewic J, Buszewski B. 2004. Some considerations about bioindicators in environmental monitoring. Polish Journal of Environmental Studies. 13(5): 453-462.
- Hogan JW. 1970. Water temperature as a source of variation in specific activity of brain acetylcholinesterase of bluegills. Bulletin of Environmental Contamination and Toxicology. 5(4): 347-353.
- Kirby MF, Morris S, Hurst M, Kirby SJ, Neall P, Tylor T, Fagg A. 2000. The use of cholinesterase activity in flounder (Platichthys flesus) muscle tissue as a biomarker of neurotoxic contamination in UK estuaries. Mar Pollut Bull. 40(9): 780-791. doi: 10.1016/S0025-326X(00)00069-2
- Kopecka J, Lehtonen KK, Baršienė J, Broeg K, Vuorinen PJ, Gercken J, Pempkowiak J. 2006. Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic). Mar Pollut Bull. 53(8-9): 406-421. doi: 10.1016/j.marpolbul.2006.03.008
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem. 193(1): 265-275.
- Pfeifer S, Schiedek D, Dippner JW. 2005. Effect of temperature and salinity on acetylcholinesterase activity, a common pollution biomarker, in Mytilus sp. from the south-western Baltic Sea. J Exp Mar Biol Ecol. 320(1): 93-103. doi: 10.1016/j.jembe.2004.12.020
- Ricciardi F, Binelli A, Provini A. 2006. Use of two biomarkers (CYP450 and acetylcholinesterase) in zebra mussel for the biomonitoring of Lake Maggiore (Northern Italy). Ecotox Environ Safe. 63(3): 406-412. doi: 10.1016/j.ecoenv.2005.02.007
- Robillard S, Beauchamp G, Laulier M. 2003. The role of abiotic factors and pesticide levels on enzymatic activity in the freshwater mussel Anodonta cygnea at three different exposure sites. Comp Biochem Phys C. 135(1): 49-59. doi: 10.1016/S1532-0456(03)00049-8
- URL:1.2018.https://www.google.com.tr/maps/@39.1667594,39.6417243,11.5z?hl=tr, 15.03.2018
- Yeşil SÖ. 2017. Tunceli İli 2016 Yılı Çevre Durum Raporu, T.C. Tunceli Valiliği Çevre ve Şehircilik İl Müdürlüğü, 80 p. [in Turkish]
Seasonal Variation of Acetylcholinesterase Activity as a Biomarker in Brain Tissue of Capoeta umbla in Pülümür Stream
Abstract
This study was carried out
between March 2015 and February 2016 with the aim of determining the seasonal
variation of acetylcholinesterase (AChE) activity in brain tissue of Capoeta
umbla (Heckel, 1843) caught from Pülümür Stream (Tunceli). The seasonal
fluctuations in the AChE activity of C. umbla which caught between the
39̊ 06' 19''N- 39̊ 36' 56''E (A) and 39̊ 11' 36''N-39̊ 41' 36''E (B)
coordinates of Pülümür Stream, were studied over a period of one year. The
enzyme activity was higher in spring and summer than in autumn and winter. In
addition, the minimum value for the AChE activity was determined in the winter
season and the maximum value was determined in the spring.
References
- Almeida JR, Gravato C, Guilhermino L. 2015. Effects of temperature in juvenile seabass (Dicentrarchus labrax L.) biomarker responses and behaviour: implications for environmental monitoring. Estuaries and Coasts. 38(1):45-55. doi: 10.1007/s12237-014-9792-7
- Barda I, Purina I, Rimsa E, Balode M. 2014. Seasonal dynamics of biomarkers in infaunal clam Macoma balthica from the Gulf of Riga (Baltic Sea). J Marine Syst. 129(2014): 150-156. doi: 10.1016/j.jmarsys.2013.05.006
- Bocchetti R, Regoli F. 2006. Seasonal variability of oxidative biomarkers, lysosomal parameters, metallothioneins and peroxisomal enzymes in the Mediterranean mussel Mytilus galloprovincialis from Adriatic Sea. Chemosphere. 65(6): 913–921. doi: 10.1016/j.chemosphere.2006.03.049
- Bocquené G, Carbamates Galgani F. 1998. Biological effects of contaminants: cholinesterase inhibition by organophosphate and carbamate compounds (No. 22). Copenhagen, Denmark: International Council for the Exploration of the Sea 19p
- Bueno-Krawcayk ACD, Guiloski IC, Piancini LDS, Azevedo JC, Ramsdorf WA, Ide AH, Guimarães ATB, Cestari MM. Silva de Assis HC. 2015. Multibiomarker in fish to evaluate a river used to water public supply. Chemosphere. 135(2015): 257-264. doi: 10.1016/j.chemosphere.2015.04.064
- Chuiko GM, Kozlovskaya VI. 1989. Seasonal fluctuations of brain acetylcholinesterase activity in perch (Perca fluviatilis L.). Physiology and Toxicology of Hydrobionts, Yaroslavl State University, Yaroslavl. p. 27-37.
- Chuiko GM, Zhelnin Y, PoD'Gornaya VA. 1997. Seasonal fluctuations in brain acetylcholinesterase activity and soluble protein content in roach (Rutilus rutilus L.): a freshwater fish from northwest Russia. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology. 117(3): 251-257. doi: 10.1016/S0742-8413(97)00068-6
- Cikcikoglu Yildirim N, Yildirim N, Danabas D, Danabas S. 2014. Use of acetylcholinesterase, glutathione S-transferase and cytochrome P450 1A1 in Capoeta umbla as biomarkers for monitoring of pollution in Uzuncayir Dam Lake (Tunceli, Turkey). Environmental toxicology and pharmacology. 37(3): 1169-1176. doi: 10.1016/j.etap.2014.04.001
- Ellman GL, Courtney KD, Andres Jr V, Featherstone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology. 7(2): 88-95. doi: 10.1016/0006-2952(61)90145-9
- Forget J, Beliaeff B, Bocquene G. 2003. Acetylcholinesterase activity in copepods (Tigriopus brevicornis) from the Vilaine River estuary, France, as a biomarker of neurotoxic contaminants. Aquatic Toxicology. 62(3): 195-204. doi: 10.1016/S0166-445X(02)00084-X
- Fulton MH, Key PB. 2001. Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environmental Toxicology and Chemistry. 20(1): 37-45. doi: 10.1002/etc.5620200104
- Gadzala-Kopciuch R, Berecka B, Bartoszewic J, Buszewski B. 2004. Some considerations about bioindicators in environmental monitoring. Polish Journal of Environmental Studies. 13(5): 453-462.
- Hogan JW. 1970. Water temperature as a source of variation in specific activity of brain acetylcholinesterase of bluegills. Bulletin of Environmental Contamination and Toxicology. 5(4): 347-353.
- Kirby MF, Morris S, Hurst M, Kirby SJ, Neall P, Tylor T, Fagg A. 2000. The use of cholinesterase activity in flounder (Platichthys flesus) muscle tissue as a biomarker of neurotoxic contamination in UK estuaries. Mar Pollut Bull. 40(9): 780-791. doi: 10.1016/S0025-326X(00)00069-2
- Kopecka J, Lehtonen KK, Baršienė J, Broeg K, Vuorinen PJ, Gercken J, Pempkowiak J. 2006. Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic). Mar Pollut Bull. 53(8-9): 406-421. doi: 10.1016/j.marpolbul.2006.03.008
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem. 193(1): 265-275.
- Pfeifer S, Schiedek D, Dippner JW. 2005. Effect of temperature and salinity on acetylcholinesterase activity, a common pollution biomarker, in Mytilus sp. from the south-western Baltic Sea. J Exp Mar Biol Ecol. 320(1): 93-103. doi: 10.1016/j.jembe.2004.12.020
- Ricciardi F, Binelli A, Provini A. 2006. Use of two biomarkers (CYP450 and acetylcholinesterase) in zebra mussel for the biomonitoring of Lake Maggiore (Northern Italy). Ecotox Environ Safe. 63(3): 406-412. doi: 10.1016/j.ecoenv.2005.02.007
- Robillard S, Beauchamp G, Laulier M. 2003. The role of abiotic factors and pesticide levels on enzymatic activity in the freshwater mussel Anodonta cygnea at three different exposure sites. Comp Biochem Phys C. 135(1): 49-59. doi: 10.1016/S1532-0456(03)00049-8
- URL:1.2018.https://www.google.com.tr/maps/@39.1667594,39.6417243,11.5z?hl=tr, 15.03.2018
- Yeşil SÖ. 2017. Tunceli İli 2016 Yılı Çevre Durum Raporu, T.C. Tunceli Valiliği Çevre ve Şehircilik İl Müdürlüğü, 80 p. [in Turkish]
There are 21 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | August 17, 2018 |
| Published in Issue | Year 2018Volume: 4 Issue: 2 |