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OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS

Year 2016, Volume: 50 Issue: 2, 1 - 9, 01.04.2016

Burcu Çakılcı Özdemir Bülent Kurtiş Gülay Tüter Burcu Şengüven Benay Yıldırım Gönen Özcan

https://doi.org/10.17096/jiufd.40536

Abstract

Purpose: Platelet-rich plasma (PRP) is a novel method for transferring autogenous growth factors to the wound area. The aim of this study was to evaluate the efficacy of double-application of PRP (DA-PRP) on bone healing in rabbit cranial defects by examining osteonectin (ON) and osteocalcin (OC) expression.
Materials and Methods: Twenty-eight rabbits, each with two surgically prepared calvarial bone defects, were included in this study and divided into six groups: The defects (N=56) were treated with either a single-application of PRP (SA-PRP) (n=10), a combination of SA-PRP and betatricalciumphosphate (SA-PRP+β-TCP) (n=10), only DAPRP (n=8), both DA-PRP and beta-tricalciumphosphate (DA-PRP+β-TCP) (n=8), only beta-tricalciumphosphate (β-TCP) (n=10), or controls (n=10). The animals were sacrificed at 30th day postoperatively and samples were immunohistochemically examined for ON and OC expressions.
Results: It was determined that DA-PRP did not significantly improve the ON and OC percentages achieved by SA-PRP or the controls. The three groups treated with β-TCP showed a higher percentage of ON than those treated without β-TCP (p<0.05). The β-TCP treated groups and SA-PRP group demonstrated higher OC percentage than DA-PRP and control groups (p<0.05).
Conclusion: The present findings suggest that DAPRP did not have a significant effect on the healing of non-critical size rabbit cranial bone defects.

Keywords

Platelet-Rich Plasma osteonectin; osteocalcin; wound healing; osteogenesis

References

  • Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85(6):638-646.
  • Tsay RC, Vo J, Burke A, Eisig SB, Lu HH, Landesberg R. Differential growth factor retention by platelet-rich plasma composites. J Oral Maxillofac Surg 2005;63(4):521-528.
  • Kovacs K, Velich N, Huszar T, Szabo G, Semjen G, Reiczigel J, Suba Z. Comparative study of beta-tricalcium phosphate mixed with platelet-rich plasma versus beta-tricalcium phosphate, a bone substitute material in dentistry. Acta Vet Hung 2003;51(4):475-484.
  • Monov G, Fuerst G, Tepper G, Watzak G, Zechner W, Watzek G. The effect of platelet-rich plasma upon implant stability measured by resonance frequency analysis in the lower anterior mandibles. Clin Oral Implants Res 2005;16(4):461-465.
  • Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Kenney EB. Comparison of platelet-rich plasma, bovine porous bone mineral, and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: A reentry study. J Periodontol 2002;73(2):198-205.
  • Anitua E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14(4):529-535.
  • Yazawa M, Ogata H, Kimura A, Nakajima T, Mori T, Watanabe N. Basic studies on the bone formation ability by platelet rich plasma in rabbits. J Craniofac Surg 2004;15(3):439-446.
  • Kovacs K, Velich N, Huszar T, Fenyves B, Suba Z, Szabo G. Histomorphometric and densitometric evaluation of the effects of platelet-rich plasma on the remodeling of beta-tricalcium phosphate in beagle dogs. J Craniofac Surg 2005;16(1):150-154.
  • Raghoebar GM, Schortinghuis J, Liem RS, Ruben JL, van der Wal JE, Vissink A. Does platelet-rich plasma promote remodeling of autologous bone grafts used for augmentation of the maxillary sinus floor? Clin Oral Implants Res 2005;16(3):349-356.
  • Thorwarth M, Rupprecht S, Falk S, Felszeghy E, Wiltfang J, Schlegel KA. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials 2005;26(15):2575-2584.
  • Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B, Moroni M, Carabelli A. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 2004;30(2):145-151.
  • Driver VR, Hanft J, Fylling CP, Beriou JM, Autologel Diabetic Foot Ulcer Study G. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage 2006;52(6):68-70, 72, 74 passim.
  • Redaelli A, Romano D, Marciano A. Face and neck revitalization with platelet-rich plasma (prp): Clinical outcome in a series of 23 consecutively treated patients. J Drugs Dermatol 9(5):466-472.
  • Kon E, Buda R, Filardo G, Di Martino A, Timoncini A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M. Platelet-rich plasma: Intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc 18(4):472-479.
  • Ozdemir B, Kurtis B, Tuter G, Senguven B, Tokman B, Pinar-Ozdemir S, Demirel I, Ozcan G. Double-application of platelet-rich plasma on bone healing in rabbits. Med Oral Patol Oral Cir Bucal 17(1):e171-177.
  • Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-rich plasma in rabbit cranial defects: A pilot study. J Oral Maxillofac Surg 2002;60(10):1176-1181.
  • Merten HA, Wiltfang J, Grohmann U, Hoenig JF. Intraindividual comparative animal study of alpha- and beta-tricalcium phosphate degradation in conjunction with simultaneous insertion of dental implants. J Craniofac Surg 2001;12(1):59-68.
  • Christenson RH. Biochemical markers of bone metabolism: An overview. Clin Biochem 1997;30(8):573-593.
  • Heinegard D, Oldberg A. Structure and biology of cartilage and bone matrix noncollagenous macromolecules. FASEB J 1989;3(9):2042-2051.
  • Termine JD, Kleinman HK, Whitson SW, Conn KM, McGarvey ML, Martin GR. Osteonectin, a bone-specific protein linking mineral to collagen. Cell 1981;26(1 Pt 1):99-105.
  • Swaminathan R. Biochemical markers of bone turnover. Clin Chim Acta 2001;313(1-2):95-105.
  • Lieberman JR, Daluiski A, Einhorn TA. The role of growth factors in the repair of bone. Biology and clinical applications. J Bone Joint Surg Am 2002;84-A(6):1032-1044.
  • Fisher LW, Termine JD. Noncollagenous proteins influencing the local mechanisms of calcification. Clin Orthop Relat Res 1985, 200):362-385.
  • Otsuka K, Yao KL, Wasi S, Tung PS, Aubin JE, Sodek J, Termine JD. Biosynthesis of osteonectin by fetal porcine calvarial cells in vitro. J Biol Chem 1984;259(15):9805-9812.
  • Kroese-Deutman HC, Wolke JG, Spauwen PH, Jansen JA. Closing capacity of cranial bone defects using porous calcium phosphate cement implants in a rabbit animal model. J Biomed Mater Res A 2006;79(3):503-511.
  • Zhu JX, Sasano Y, Takahashi I, Mizoguchi I, Kagayama M. Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. Histochem J 2001;33(1):25-35.
  • Clarke B. Normal bone anatomy and physiology. Clin J Am Soc Nephrol 2008;3 Suppl 3(S131-139.
  • Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA. Mineralization and the expression of matrix proteins during in vivo bone development. Calcif Tissue Int 1998;62(1):74-82.
  • Wiesmann HP, Meyer U, Plate U, Hohling HJ. Aspects of collagen mineralization in hard tissue formation. Int Rev Cytol 2005;242:121-156.
  • Boskey AL, Gadaleta S, Gundberg C, Doty SB, Ducy P, Karsenty G. Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin. Bone 1998;23(3):187-196.
  • Hunter GK, Hauschka PV, Poole AR, Rosenberg LC, Goldberg HA. Nucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins. Biochem J 1996;317(1):59-64.
  • Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 1990;143(3):420-430.
  • Velich N, Nemeth Z, Hrabak K, Suba Z, Szabo G. Repair of bony defect with combination biomaterials. J Craniofac Surg 2004;15(1):11-15.
  • Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze-Mosgau S, Zimmermann R, Schlegel KA. Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment. Clin Oral Implants Res 2004;15(2):187-193.
  • Harnack L, Boedeker RH, Kurtulus I, Boehm S, Gonzales J, Meyle J. Use of platelet-rich plasma in periodontal surgery--a prospective randomised double blind clinical trial. Clin Oral Investig 2009;13(2):179-187.
  • Ozdemir B, Okte E. Treatment of intrabony defects with beta-tricalciumphosphate alone and in combination with platelet-rich plasma. J Biomed Mater Res B Appl Biomater 2012;100(4):976-983.
  • Kondo N, Ogose A, Tokunaga K, Ito T, Arai K, Kudo N, Inoue H, Irie H, Endo N. Bone formation and resorption of highly purified beta-tricalcium phosphate in the rat femoral condyle. Biomaterials 2005;26(28):5600-5608.

Year 2016, Volume: 50 Issue: 2, 1 - 9, 01.04.2016

Burcu Çakılcı Özdemir Bülent Kurtiş Gülay Tüter Burcu Şengüven Benay Yıldırım Gönen Özcan

https://doi.org/10.17096/jiufd.40536

Abstract

References

  • Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85(6):638-646.
  • Tsay RC, Vo J, Burke A, Eisig SB, Lu HH, Landesberg R. Differential growth factor retention by platelet-rich plasma composites. J Oral Maxillofac Surg 2005;63(4):521-528.
  • Kovacs K, Velich N, Huszar T, Szabo G, Semjen G, Reiczigel J, Suba Z. Comparative study of beta-tricalcium phosphate mixed with platelet-rich plasma versus beta-tricalcium phosphate, a bone substitute material in dentistry. Acta Vet Hung 2003;51(4):475-484.
  • Monov G, Fuerst G, Tepper G, Watzak G, Zechner W, Watzek G. The effect of platelet-rich plasma upon implant stability measured by resonance frequency analysis in the lower anterior mandibles. Clin Oral Implants Res 2005;16(4):461-465.
  • Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Kenney EB. Comparison of platelet-rich plasma, bovine porous bone mineral, and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: A reentry study. J Periodontol 2002;73(2):198-205.
  • Anitua E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14(4):529-535.
  • Yazawa M, Ogata H, Kimura A, Nakajima T, Mori T, Watanabe N. Basic studies on the bone formation ability by platelet rich plasma in rabbits. J Craniofac Surg 2004;15(3):439-446.
  • Kovacs K, Velich N, Huszar T, Fenyves B, Suba Z, Szabo G. Histomorphometric and densitometric evaluation of the effects of platelet-rich plasma on the remodeling of beta-tricalcium phosphate in beagle dogs. J Craniofac Surg 2005;16(1):150-154.
  • Raghoebar GM, Schortinghuis J, Liem RS, Ruben JL, van der Wal JE, Vissink A. Does platelet-rich plasma promote remodeling of autologous bone grafts used for augmentation of the maxillary sinus floor? Clin Oral Implants Res 2005;16(3):349-356.
  • Thorwarth M, Rupprecht S, Falk S, Felszeghy E, Wiltfang J, Schlegel KA. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials 2005;26(15):2575-2584.
  • Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B, Moroni M, Carabelli A. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 2004;30(2):145-151.
  • Driver VR, Hanft J, Fylling CP, Beriou JM, Autologel Diabetic Foot Ulcer Study G. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage 2006;52(6):68-70, 72, 74 passim.
  • Redaelli A, Romano D, Marciano A. Face and neck revitalization with platelet-rich plasma (prp): Clinical outcome in a series of 23 consecutively treated patients. J Drugs Dermatol 9(5):466-472.
  • Kon E, Buda R, Filardo G, Di Martino A, Timoncini A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M. Platelet-rich plasma: Intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc 18(4):472-479.
  • Ozdemir B, Kurtis B, Tuter G, Senguven B, Tokman B, Pinar-Ozdemir S, Demirel I, Ozcan G. Double-application of platelet-rich plasma on bone healing in rabbits. Med Oral Patol Oral Cir Bucal 17(1):e171-177.
  • Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-rich plasma in rabbit cranial defects: A pilot study. J Oral Maxillofac Surg 2002;60(10):1176-1181.
  • Merten HA, Wiltfang J, Grohmann U, Hoenig JF. Intraindividual comparative animal study of alpha- and beta-tricalcium phosphate degradation in conjunction with simultaneous insertion of dental implants. J Craniofac Surg 2001;12(1):59-68.
  • Christenson RH. Biochemical markers of bone metabolism: An overview. Clin Biochem 1997;30(8):573-593.
  • Heinegard D, Oldberg A. Structure and biology of cartilage and bone matrix noncollagenous macromolecules. FASEB J 1989;3(9):2042-2051.
  • Termine JD, Kleinman HK, Whitson SW, Conn KM, McGarvey ML, Martin GR. Osteonectin, a bone-specific protein linking mineral to collagen. Cell 1981;26(1 Pt 1):99-105.
  • Swaminathan R. Biochemical markers of bone turnover. Clin Chim Acta 2001;313(1-2):95-105.
  • Lieberman JR, Daluiski A, Einhorn TA. The role of growth factors in the repair of bone. Biology and clinical applications. J Bone Joint Surg Am 2002;84-A(6):1032-1044.
  • Fisher LW, Termine JD. Noncollagenous proteins influencing the local mechanisms of calcification. Clin Orthop Relat Res 1985, 200):362-385.
  • Otsuka K, Yao KL, Wasi S, Tung PS, Aubin JE, Sodek J, Termine JD. Biosynthesis of osteonectin by fetal porcine calvarial cells in vitro. J Biol Chem 1984;259(15):9805-9812.
  • Kroese-Deutman HC, Wolke JG, Spauwen PH, Jansen JA. Closing capacity of cranial bone defects using porous calcium phosphate cement implants in a rabbit animal model. J Biomed Mater Res A 2006;79(3):503-511.
  • Zhu JX, Sasano Y, Takahashi I, Mizoguchi I, Kagayama M. Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. Histochem J 2001;33(1):25-35.
  • Clarke B. Normal bone anatomy and physiology. Clin J Am Soc Nephrol 2008;3 Suppl 3(S131-139.
  • Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA. Mineralization and the expression of matrix proteins during in vivo bone development. Calcif Tissue Int 1998;62(1):74-82.
  • Wiesmann HP, Meyer U, Plate U, Hohling HJ. Aspects of collagen mineralization in hard tissue formation. Int Rev Cytol 2005;242:121-156.
  • Boskey AL, Gadaleta S, Gundberg C, Doty SB, Ducy P, Karsenty G. Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin. Bone 1998;23(3):187-196.
  • Hunter GK, Hauschka PV, Poole AR, Rosenberg LC, Goldberg HA. Nucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins. Biochem J 1996;317(1):59-64.
  • Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 1990;143(3):420-430.
  • Velich N, Nemeth Z, Hrabak K, Suba Z, Szabo G. Repair of bony defect with combination biomaterials. J Craniofac Surg 2004;15(1):11-15.
  • Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze-Mosgau S, Zimmermann R, Schlegel KA. Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment. Clin Oral Implants Res 2004;15(2):187-193.
  • Harnack L, Boedeker RH, Kurtulus I, Boehm S, Gonzales J, Meyle J. Use of platelet-rich plasma in periodontal surgery--a prospective randomised double blind clinical trial. Clin Oral Investig 2009;13(2):179-187.
  • Ozdemir B, Okte E. Treatment of intrabony defects with beta-tricalciumphosphate alone and in combination with platelet-rich plasma. J Biomed Mater Res B Appl Biomater 2012;100(4):976-983.
  • Kondo N, Ogose A, Tokunaga K, Ito T, Arai K, Kudo N, Inoue H, Irie H, Endo N. Bone formation and resorption of highly purified beta-tricalcium phosphate in the rat femoral condyle. Biomaterials 2005;26(28):5600-5608.
There are 37 citations in total.

Details

Journal Section Original Research Articles
Authors

Burcu Çakılcı Özdemir

Bülent Kurtiş This is me

Gülay Tüter

Burcu Şengüven This is me

Benay Yıldırım

Gönen Özcan This is me

Publication Date April 1, 2016
Published in Issue Year 2016 Volume: 50 Issue: 2

Cite

APA Çakılcı Özdemir, B., Kurtiş, B., Tüter, G., Şengüven, B., et al. (2016). OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS. Journal of Istanbul University Faculty of Dentistry, 50(2), 1-9. https://doi.org/10.17096/jiufd.40536
AMA Çakılcı Özdemir B, Kurtiş B, Tüter G, Şengüven B, Yıldırım B, Özcan G. OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS. J Istanbul Univ Fac Dent. April 2016;50(2):1-9. doi:10.17096/jiufd.40536
Chicago Çakılcı Özdemir, Burcu, Bülent Kurtiş, Gülay Tüter, Burcu Şengüven, Benay Yıldırım, and Gönen Özcan. “OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS”. Journal of Istanbul University Faculty of Dentistry 50, no. 2 (April 2016): 1-9. https://doi.org/10.17096/jiufd.40536.
EndNote Çakılcı Özdemir B, Kurtiş B, Tüter G, Şengüven B, Yıldırım B, Özcan G (April 1, 2016) OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS. Journal of Istanbul University Faculty of Dentistry 50 2 1–9.
IEEE B. Çakılcı Özdemir, B. Kurtiş, G. Tüter, B. Şengüven, B. Yıldırım, and G. Özcan, “OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS”, J Istanbul Univ Fac Dent, vol. 50, no. 2, pp. 1–9, 2016, doi: 10.17096/jiufd.40536.
ISNAD Çakılcı Özdemir, Burcu et al. “OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS”. Journal of Istanbul University Faculty of Dentistry 50/2 (April 2016), 1-9. https://doi.org/10.17096/jiufd.40536.
JAMA Çakılcı Özdemir B, Kurtiş B, Tüter G, Şengüven B, Yıldırım B, Özcan G. OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS. J Istanbul Univ Fac Dent. 2016;50:1–9.
MLA Çakılcı Özdemir, Burcu et al. “OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS”. Journal of Istanbul University Faculty of Dentistry, vol. 50, no. 2, 2016, pp. 1-9, doi:10.17096/jiufd.40536.
Vancouver Çakılcı Özdemir B, Kurtiş B, Tüter G, Şengüven B, Yıldırım B, Özcan G. OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS. J Istanbul Univ Fac Dent. 2016;50(2):1-9.
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