The effect of Lycium barbarum on reproductive system and the expression of CRISP-1 protein in experimentally diabetic male rats
Abstract
Aim: The main purpose of this study is to investigate the effects of Lycium barbarum polysaccharides (LBP) on the male reproductive system and Crisp-1 protein expression in experimentally diabetic Wistar Albino male rats.
Material and Method: In this study Wistar Albino male rats (3-4 months old) were randomly selected and divided into four groups; Control, LBP control (only LBP), Diabetic and Treatment (diabetic + LBP) group. For the experimental diabetes model, a single dose of 55 mg/kg STZ was injected intraperitoneally. In the treatment group, the diabetic rats were administered with 200 mg/kg of LBP by gastric gavage for 15 consecutive days.
Results: In histological examinations, increased intertubular connective tissue, congestion, vacuolization and edema were observed in testicular tissues of the diabetic group. The histopathological changes were improved after LBP treatment. Also, the number of total sperm count and sperm motility were significantly increased in the treatment group. Our biochemical analysis results showed that the serum testosterone level were significantly increased and serum MDA level were significantly decreased after treatment with LBP. Compared to the diabetic group, the apoptotic cells were decreased in the treatment group. Crisp-1 protein expression was increased in the treatment group, Crisp-1 possitive vesicle-like structures and apical blebs were also examined in the epididymal tissues.
Conclusion: It is concluded that Lycium barbarum polysaccharides have a therapeutic effect on the male reproductive damages of diabetes and also enhances Crisp-1 protein expression in the epididymis.
Keywords
antioxidant crisp-1 protein cysteine-rich secretory protein diabetes mellitus infertility lycium barbarum
Supporting Institution
Abant Izzet Baysal University Scientific Research Projects
Project Number
Project ID: 2016.08.03.1098
Thanks
We would like to thank and show our respects to our co-author Dr. Buket KIN TEKCE who passed away in September of 2020. She was a kind person and a great instructor at the Department of Medical Biochemistry at Abant Izzet Baysal University.
References
- Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998; 15: 539-53.
- Rytter E, Vessby B, Asgard R, et al. Glycemic status in relation to oxidative stress and inflammation in well-controlled type-2 diabetes subjects. British J Nutri 2009; 101:1423-26.
- Kuzuya T, Nakagawa S, Satoh J, et al. Report of the Committee on the classification and diagnostic criteria of diabetes mellitus. Diabetes Res Clin Pract 2002; 55: 65-85.
- Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79: 829-43.
- Agbaje IM, Rogers DA, McVicar CM, et al. Insulin dependent diabetes mellitus: implications for male reproductive function. Hum Reprod 2007; 22: 1871-77.
- Kilarkaje N, Al-Hussaini H, Al-Bader MM. Diabetes-induced DNA damage and apoptosis are associated with poly (ADP ribose) polymerase 1 inhibition in the rat testis. Eur J Pharmacol 2014; 737: 29-40.
- Cavallini G. Male idiopathic oligoasthenoteratozoospermia. Asian J Androl 2006; 8: 143-57.
- Cameo MS, Blaquier JA. Androgen-controlled specific proteins in rat epididymis. J Endocr 1976; 69: 47-55.
- Kohane AC, Cameo MS, Piñeiro L, Garberi JC, Blaquier JA. Distribution and site of production of specific proteins in the rat epididymis. Biol Reprod 1980; 23: 181-7.
- Kohane AC, González Echeverría FM, Piñeiro L, Blaquier JA. Interaction of proteins of epididymal origin with spermatozoa. Biol Reprod 1980; 23: 737-42.
- Cohen DJ, Rochwerger L, Ellerman DA, et al. Relationship between the association of rat epididymal protein “DE” with spermatozoa and the behavior and function of the protein. Mol Reprod Dev 2000; 56: 180-8.
- Da Ros VG, Munoz MW, Battistone MA, et al. From the epididymis to the egg: participation of CRISP proteins in mammal fertilization. Asian J Androl 2015; 17: 711-5.
- Cohen DJ, Da Ros VG, Busso D, et al. Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation. Asian J Androl 2007; 9:5 28-32.
- Maldera JA, Munoz MW, Chirinos M, et al. Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3. Mol Hum Reprod 2014; 20: 341-9.
- Cohen DJ, Ellerman DA, Cuasnicu PS. Mammalian sperm-egg fusion: evidence that epididymal protein DE plays a role in gamete fusion. Biol Reprod 2000; 63: 462-8.
- Cohen DJ, Maldera JA, Munoz MW et al. Cysteine rich secretory proteins and their role in mammalian fertilization. Biol Res 2011; 44: 135-8.
- Da Ros VG, Maldera JA, Willis WD, et al. Impaired sperm fertilizing ability in mice lacking Cysteine-Rich Secretory Protein 1 (CRISP1). Dev Biol 2008; .320: 12-8.
- Dursun R, Zengin Y, Gunduz E, et al. The protective effect of goji berry extract in ischemic reperfusion in testis torsion. Int J Clin Exp Med 2015; 8: 2727-33.
- Zhang C, Wang A, Sun X, et al. Protective effect of Lycium barbarum Polysaccharides on testis spermatogenic injury induced by Bisphenol-A in mice. Evidence-Based Complementary and Alternative Medicine 2013.
- Jing L, Cui G, Feng Q, Xiao Y. Evaluation of hypoglycemic activity of the polysaccharides extracted from Lycium barbarum. Afr J Med Med Sci 2009; 6: 579-84.
- Luo Q, Li Z, Huang X, et al. Lycium barbarum polysaccharides: Protective effects against heat-induced damage of rat testes and H2O2-induced DNA damage in mouse testicular cells and beneficial effect on sexual behavior and reproductive function of hemicastrated rats. Life Sci 2006; 79: 613-21.
- Luo Q, Li J, Cui X, et al. The effect of Lycium barbarum polysaccharides on the male rats .30 reproductive system and spermatogenic cell apoptosis exposed to low-dose ionizing irradiation. J Ethnopharmacol 2014; 154: 249-58.
- Huang XL, Yang ML, Wu XM. Study on protective effect of Lycium barbarum polysaccharides on testis tissue damage of male rats. China Public Health 2003.
- Şendoğdu N, Aslan M, Orhan DD, Ergun F, Yeşilada E. Antidiabetic and antioxidant effects of Vitis vinifera L. leaves in streptozotocin-diabetic rats. Turkish J Pharm Sci 2006; 3, 7-18.
- Jin M, Huang Q, Zhao K, Shang P. Biological activities and potential health benefit effects of polysaccharides isolated from Lycium barbarum L. Int J Biol Macromol 2013; 54: 16-23.
- Amidu N, Owiredu WK, Alidu H, et al. Association between metabolic syndrome and sexual dysfunction among men with clinically diagnosed diabetes. Diabetol Metab Syndrome 2013; 5: 1-8.
- Abbasi Z, Tabatabaei SR, Mazaheri Y, Barati F, Morovvati H. Effects of sesame oil on the reproductive parameters of diabetes mellitus-induced male rats. World J Mens Health 2013; 31: 141-9.
- Ahangarpour A, Oroojan AA, Heidari H, Ehsan G, Rashidi Nooshabadi MR. Effects of hydro-alcoholic extract of rhus coriaria (Sumac) seeds on reproductive complications of nicotinamide-streptozotocin induced type-2 diabetes in male mice. World J Mens Health 2014; 32: 151-8.
- Simon L, Brunborg G, Stevenson M, Lutton D, McManus J, Lewis SE. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod 2010; 25: 1594-1608.
- Kohane AC, González Echeverría FM, Piñeiro L, Blaquier JA. Interaction of proteins of epididymal origin with spermatozoa. Biol Reprod 1980; 23: 737-42.
- Shi GJ, Zheng J, Wu J, et al. Beneficial effects of Lycium barbarum polysaccharide on spermatogenesis by improving antioxidant activity and inhibiting apoptosis in streptozotocin-induced diabetic male mice. Food Function 2017; 8, 1215-26.
- Ismael ZK, AL-Anbari LA, Mossa HA. Relationship of FSH, LH, DHEA and testosterone levels in serum with sperm function parameters in infertile men. J Pharm Sci Res 2017; 9 2056-61.
- Qian L, Yu S. Protective effect of polysaccharides from Lycium barbarum on spermatogenesis of mice with impaired reproduction system induced by cyclophosphamide. Am J Reprod Immunol 2006; 76: 383-85.
- Lei X, Huo P, Wang Y, et al. Lycium barbarum polysaccharides improve testicular spermatogenic function in streptozotocin-induced diabetic rats. Front Endocrinol (Lausanne). 2020;11:164.
- Cuasnicú PS, Echeverría FG, Piazza AD, Cameo MS, Blaquier JA. Antibodies against epididymal glycoproteins block fertilizing ability in rat. J Reprod Fertil 1984; 72, 467-471.
- Busso D, Cohen DJ, Maldera JA, Dematteis A, Cuasnicu PS. A novel function for CRISP1 in rodent fertilization: involvement in sperm-zona pellucida interaction. Biol Reprod 2007; 77: 848-54.
- Sullivan R, Saez F, Girouard J, Frenette G. Role of exosomes in sperm maturation during the transit along the male reproductive tract. Blood Cells Mol Dis 2005; 35, 1-10.
- Sullivan R, Frenette G, Girouard J. Epididymosomes are involved in the acquisition of new sperm proteins during epididymal sperm transit. Asian J Androl 2007; 9: 483-91.
- Thimon V, Frenette G, Saez F, Thabet M, Sullivan R. Protein composition of human epididymosomes collected during surgical vasectomy reversal: a proteomic and genomic approach. Hum Reprod 2008; 23: 1698-707.
Abstract
Project Number
Project ID: 2016.08.03.1098
References
- Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998; 15: 539-53.
- Rytter E, Vessby B, Asgard R, et al. Glycemic status in relation to oxidative stress and inflammation in well-controlled type-2 diabetes subjects. British J Nutri 2009; 101:1423-26.
- Kuzuya T, Nakagawa S, Satoh J, et al. Report of the Committee on the classification and diagnostic criteria of diabetes mellitus. Diabetes Res Clin Pract 2002; 55: 65-85.
- Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79: 829-43.
- Agbaje IM, Rogers DA, McVicar CM, et al. Insulin dependent diabetes mellitus: implications for male reproductive function. Hum Reprod 2007; 22: 1871-77.
- Kilarkaje N, Al-Hussaini H, Al-Bader MM. Diabetes-induced DNA damage and apoptosis are associated with poly (ADP ribose) polymerase 1 inhibition in the rat testis. Eur J Pharmacol 2014; 737: 29-40.
- Cavallini G. Male idiopathic oligoasthenoteratozoospermia. Asian J Androl 2006; 8: 143-57.
- Cameo MS, Blaquier JA. Androgen-controlled specific proteins in rat epididymis. J Endocr 1976; 69: 47-55.
- Kohane AC, Cameo MS, Piñeiro L, Garberi JC, Blaquier JA. Distribution and site of production of specific proteins in the rat epididymis. Biol Reprod 1980; 23: 181-7.
- Kohane AC, González Echeverría FM, Piñeiro L, Blaquier JA. Interaction of proteins of epididymal origin with spermatozoa. Biol Reprod 1980; 23: 737-42.
- Cohen DJ, Rochwerger L, Ellerman DA, et al. Relationship between the association of rat epididymal protein “DE” with spermatozoa and the behavior and function of the protein. Mol Reprod Dev 2000; 56: 180-8.
- Da Ros VG, Munoz MW, Battistone MA, et al. From the epididymis to the egg: participation of CRISP proteins in mammal fertilization. Asian J Androl 2015; 17: 711-5.
- Cohen DJ, Da Ros VG, Busso D, et al. Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation. Asian J Androl 2007; 9:5 28-32.
- Maldera JA, Munoz MW, Chirinos M, et al. Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3. Mol Hum Reprod 2014; 20: 341-9.
- Cohen DJ, Ellerman DA, Cuasnicu PS. Mammalian sperm-egg fusion: evidence that epididymal protein DE plays a role in gamete fusion. Biol Reprod 2000; 63: 462-8.
- Cohen DJ, Maldera JA, Munoz MW et al. Cysteine rich secretory proteins and their role in mammalian fertilization. Biol Res 2011; 44: 135-8.
- Da Ros VG, Maldera JA, Willis WD, et al. Impaired sperm fertilizing ability in mice lacking Cysteine-Rich Secretory Protein 1 (CRISP1). Dev Biol 2008; .320: 12-8.
- Dursun R, Zengin Y, Gunduz E, et al. The protective effect of goji berry extract in ischemic reperfusion in testis torsion. Int J Clin Exp Med 2015; 8: 2727-33.
- Zhang C, Wang A, Sun X, et al. Protective effect of Lycium barbarum Polysaccharides on testis spermatogenic injury induced by Bisphenol-A in mice. Evidence-Based Complementary and Alternative Medicine 2013.
- Jing L, Cui G, Feng Q, Xiao Y. Evaluation of hypoglycemic activity of the polysaccharides extracted from Lycium barbarum. Afr J Med Med Sci 2009; 6: 579-84.
- Luo Q, Li Z, Huang X, et al. Lycium barbarum polysaccharides: Protective effects against heat-induced damage of rat testes and H2O2-induced DNA damage in mouse testicular cells and beneficial effect on sexual behavior and reproductive function of hemicastrated rats. Life Sci 2006; 79: 613-21.
- Luo Q, Li J, Cui X, et al. The effect of Lycium barbarum polysaccharides on the male rats .30 reproductive system and spermatogenic cell apoptosis exposed to low-dose ionizing irradiation. J Ethnopharmacol 2014; 154: 249-58.
- Huang XL, Yang ML, Wu XM. Study on protective effect of Lycium barbarum polysaccharides on testis tissue damage of male rats. China Public Health 2003.
- Şendoğdu N, Aslan M, Orhan DD, Ergun F, Yeşilada E. Antidiabetic and antioxidant effects of Vitis vinifera L. leaves in streptozotocin-diabetic rats. Turkish J Pharm Sci 2006; 3, 7-18.
- Jin M, Huang Q, Zhao K, Shang P. Biological activities and potential health benefit effects of polysaccharides isolated from Lycium barbarum L. Int J Biol Macromol 2013; 54: 16-23.
- Amidu N, Owiredu WK, Alidu H, et al. Association between metabolic syndrome and sexual dysfunction among men with clinically diagnosed diabetes. Diabetol Metab Syndrome 2013; 5: 1-8.
- Abbasi Z, Tabatabaei SR, Mazaheri Y, Barati F, Morovvati H. Effects of sesame oil on the reproductive parameters of diabetes mellitus-induced male rats. World J Mens Health 2013; 31: 141-9.
- Ahangarpour A, Oroojan AA, Heidari H, Ehsan G, Rashidi Nooshabadi MR. Effects of hydro-alcoholic extract of rhus coriaria (Sumac) seeds on reproductive complications of nicotinamide-streptozotocin induced type-2 diabetes in male mice. World J Mens Health 2014; 32: 151-8.
- Simon L, Brunborg G, Stevenson M, Lutton D, McManus J, Lewis SE. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod 2010; 25: 1594-1608.
- Kohane AC, González Echeverría FM, Piñeiro L, Blaquier JA. Interaction of proteins of epididymal origin with spermatozoa. Biol Reprod 1980; 23: 737-42.
- Shi GJ, Zheng J, Wu J, et al. Beneficial effects of Lycium barbarum polysaccharide on spermatogenesis by improving antioxidant activity and inhibiting apoptosis in streptozotocin-induced diabetic male mice. Food Function 2017; 8, 1215-26.
- Ismael ZK, AL-Anbari LA, Mossa HA. Relationship of FSH, LH, DHEA and testosterone levels in serum with sperm function parameters in infertile men. J Pharm Sci Res 2017; 9 2056-61.
- Qian L, Yu S. Protective effect of polysaccharides from Lycium barbarum on spermatogenesis of mice with impaired reproduction system induced by cyclophosphamide. Am J Reprod Immunol 2006; 76: 383-85.
- Lei X, Huo P, Wang Y, et al. Lycium barbarum polysaccharides improve testicular spermatogenic function in streptozotocin-induced diabetic rats. Front Endocrinol (Lausanne). 2020;11:164.
- Cuasnicú PS, Echeverría FG, Piazza AD, Cameo MS, Blaquier JA. Antibodies against epididymal glycoproteins block fertilizing ability in rat. J Reprod Fertil 1984; 72, 467-471.
- Busso D, Cohen DJ, Maldera JA, Dematteis A, Cuasnicu PS. A novel function for CRISP1 in rodent fertilization: involvement in sperm-zona pellucida interaction. Biol Reprod 2007; 77: 848-54.
- Sullivan R, Saez F, Girouard J, Frenette G. Role of exosomes in sperm maturation during the transit along the male reproductive tract. Blood Cells Mol Dis 2005; 35, 1-10.
- Sullivan R, Frenette G, Girouard J. Epididymosomes are involved in the acquisition of new sperm proteins during epididymal sperm transit. Asian J Androl 2007; 9: 483-91.
- Thimon V, Frenette G, Saez F, Thabet M, Sullivan R. Protein composition of human epididymosomes collected during surgical vasectomy reversal: a proteomic and genomic approach. Hum Reprod 2008; 23: 1698-707.
Details
| Primary Language | English |
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| Subjects | Health Care Administration |
| Journal Section | Original Article |
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| Project Number | Project ID: 2016.08.03.1098 |
| Publication Date | May 30, 2022 |
| Published in Issue | Year 2022 Volume: 5 Issue: 3 |
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