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Genotoxicological Potentials of Different Habitat Types in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Thrace Region

Year 2024, Volume: 9 Issue: 1, 110 - 118

Begüm Boran Bengi Baycan Çiğdem Gül Murat Tosunoğlu

https://doi.org/10.35229/jaes.1398305

Abstract

Testudo hermanni species are considered near threatened globally in the wild. In this study, differential leukocyte percentages, nuclear abnormalities, and erythrocyte morphology abnormalities of Testudo hermanni populations distributed in two different habitat types in the Thrace Region were determined to whether habitats have a potential genotoxicological effect. A total of 37 tortoise specimens, 18 from an open habitat in Kırklareli/Karakoç, and 19 from an isolated habitat in Edirne/Keşan were studied. According to the results obtained from the Micronucleus Test, which detects genotoxic damage, there was no statistically significant difference between the two habitats. There were statistically significant differences in erythrocyte morphology abnormalities between two populations, such as frequencies of anucleated erythrocyte, nuclear shift and elliptical shape distortion. There were correlations between body sizes and other parameters. This is the first genotoxicological study that conducted on Testudo hermanni species in Türkiye.

Keywords

Genotoxicology Micronucleus Test Testudo hermanni Thrace Region

Ethical Statement

The necessary permissions have been obtained from the Ethics Committee of Animal Experiments of Çanakkale Onsekiz Mart University (decision no: 2023/01-06) for the studies that carried out.

Supporting Institution

Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit

Project Number

FDK-2021-3555.

Thanks

This study was supported by Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit, Project Number: FDK-2021-3555.

References

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  • Çördük, N., Hacıoğlu Doğru, N., Gül, Ç. & Tosunoğlu, M. (2022). Determination of the effects of environmental pollution on the Balkan terrapin, Mauremys rivulata (Valenciennes, 1833). Acta Veterinaria Hungarica. 70(3), 245-253.
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Trakya Bölgesi’nde Dağılış Gösteren Trakya Tosbağası (Testudo hermanni) Popülasyonlarında Habitat Tiplerinin Genotoksikoloji Potansiyelleri

Year 2024, Volume: 9 Issue: 1, 110 - 118

Begüm Boran Bengi Baycan Çiğdem Gül Murat Tosunoğlu

https://doi.org/10.35229/jaes.1398305

Abstract

Testudo hermanni türü, doğada küresel olarak nesli tehlike altında olarak kabul edilmektedir. Bu çalışmada, Trakya Bölgesi’nde iki farklı habitat tipinde bulunan Testudo hermanni popülasyonlarının diferansiyel lökosit yüzdeleri, nüklear anormallikleri ve eritrosit morfolojisi anormallikleri tespit edilerek habitatların potansiyel bir genotoksik etkiye sahip olup olmadığı belirlenmiştir. Kırklareli/Karakoç’taki açık bir habitattan 18 ve Edirne/Keşan’daki izole bir habitattan 19 olmak üzere toplam 37 kaplumbağa örneği üzerinde çalışılmıştır. Genotoksik hasarı tespit eden Mikronukleus Testi’nden elde edilen sonuçlara göre, iki habitat arasında istatistiksel olarak anlamlı bir fark bulunmamıştır. Ancak, iki popülasyon arasında çekirdeksiz eritrosit, nüklear kayma ve eliptik şekil bozukluğu gibi eritrosit morfolojisi anormalliklerinde anlamlı farklar bulunmuştur. Vücut büyüklükleri ile diğer parametreler arasında korelasyonlar tespit edilmiştir. Bu çalışma, ülkemizde Testudo hermanni türünde gerçekleştirilen ilk genotoksikolojik çalışmadır.

Keywords

Genotoksikoloji Mikronükleus Testi Testudo hermanni Trakya Bölgesi

Ethical Statement

Yapılan çalışmalar için Çanakkale Onsekiz Mart Üniversitesi Hayvan Deneyleri Etik Kurulu'ndan (karar no: 2023/01-06) gerekli izinler alınmıştır.

Supporting Institution

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FDK-2021-3555.

Thanks

Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Koordinasyon Birimi (Proje Numarası: FDK-2021-3555) tarafından desteklenmiştir.

References

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  • Aroutiounian, R., Sargsyan, A., Simonyan, A., Hovhannisyan, G. & Arakelyan, M. (2019). Application of the comet assay, micronucleus test and global DNA methylation analysis in Darevskia lizards as a sentinel organism for genotoxic monitoring of soil pollution. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 842, 117-124.
  • Babini, M.S., Bionda, C., Salas, N.E. & Martino, A.L. (2015). Health status of tadpoles and metamorphs of Rhinella arenarum (Anura, Bufonidae) that inhabit agroecosystems and its implications for land use. Ecotoxicology and Environmental Safety, 118, 118-125. DOI: 10.1016/j.ecoenv.2015.04.017.
  • Ballard, B.M. & Cheek, R. (eds.) (2003). Exotic animal medicine for the veterinary technician. Wiley and Sons / Blackwell Publishing, New York.
  • Baran, İ., Avcı, A., Kumlutaş, Y., Olgun, K. & Ilgaz, Ç. (2021). Türkiye amfibi ve sürüngenleri. Palme Yayıncılık, Ankara.
  • Barni, S., Boncompagni, E., Grosso, A., Bertone, V., Freitas, I., Fasola, M. & Fenoglio, C. (2007). Evaluation of Rana snk esculenta blood cell response to chemical stressors in the environment during the larval and adult phases. Aquatic Toxicology. 81(1), 45e54.
  • Baycan, B., Boran, B., Gül, Ç. & Tosunoğlu, M. (2022). Clinical Hematology of the Nose-Horned Viper, Vipera ammodytes (Linnaeus 1758). Reptiles & Amphibians, 29(1), 461-469.
  • Boran, B. (2022). Sarıçay (Çanakkale)’daki Çevresel Parametrelerin Pelophylax bedriagae ve Mauremys rivulata Türleri Üzerinde Hematolojik ve Genotoksikolojik Etkilerinin Belirlenmesi. Yüksek Lisans Tezi, Çanakkale Onsekiz Mart Üniversitesi, Lisansüstü Eğitim Enstitüsü, Çanakkale, Türkiye.
  • Campbell, T.W. (2004). Hematology of lower vertebrates. In: 55 th Annual Meeting of the American College of Veterinary Pathologists (ACVP) and 39 th Annual Meeting of the American Society of Clinical Pathology (ASVCP). American College of Veterinary Pathologists & American Society for Veterinary Clinical Pathology, Middleton, WI. pp. 1214-1218.
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  • Cheylan, M. (2001). Testudo hermanni Gmelin. 1798- Griechische Landschildkröten. In: Fritz. U. (Ed.). Handbuch der Reptilien und Amphibien Europas. Band 3/IIIA: Schildkröten (Testudines). I. (Bataguridae. Testudinidae. Emydidae). Wiebelsheim: Aula-Verlag. pp. 179-289.
  • Çiçek, K., Arıkan, H., Ayaz, D. & Mermer, A. (2015). Blood cells morphology of Testudo graeca and Testudo hermanni (Testudines: Testudinidae) from Turkey. Biharean Biologist, 9(2), 113-116.
  • Çördük, N., Hacıoğlu Doğru, N., Gül, Ç. & Tosunoğlu, M. (2022). Determination of the effects of environmental pollution on the Balkan terrapin, Mauremys rivulata (Valenciennes, 1833). Acta Veterinaria Hungarica. 70(3), 245-253.
  • Dusinska, M., Rundén-Pran, E., Carreira, S. C., & Saunders, M. (2012). Adverse Effects of Engineered Nanomaterials, Chapter 4-Critical Evaluation of Toxicity Tests, 63-83pp. DOI: 10.1016/b978-0-12-386940-1.00004-0.
  • Fenech, M. (2000). The in vitro micronucleus technique. Mutation Research, 455, 81-95.
  • Fenech, M., Kirsch-Volders, M., Natarajan, A.T., Surralles, J., Crott, J.W., Parry, J., Norppa, H., Eastmond, D.A., Tucker, J.D. & Thomas, P. (2011). Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells, Mutagenesis, 26(2011) 125-132, DOI: 10.1093/mutage/geq052
  • Fernández‐Chacón, A., Bertolero, A., Amengual, A., Tavecchia, G., Homar, V. & Oro, D. (2011). Spatial heterogeneity in the effects of climate change on the population dynamics of a Mediterranean tortoise. Global Change Biology, 17(10), 3075-3088.
  • Freire, E.M.X., Silva, J.M., Navoni, J.A. & Amaral, V.S. (2021). Cytogenetic analysis of nuclear abnormalities in the erythrocytes of gecko lizards (Phyllopezus periosus) collected in a semi-arid region of northeast Brazil: Possible effects of natural background radioactivity. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 865, 503346.
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There are 62 citations in total.

Details

Primary Language English
Subjects Zoology (Other)
Journal Section Articles
Authors

Begüm Boran 0000-0002-3069-7780

Bengi Baycan 0000-0002-2758-6166

Çiğdem Gül 0000-0003-4736-2677

Murat Tosunoğlu 0000-0002-9764-2477

Project Number FDK-2021-3555.
Early Pub Date March 22, 2024
Publication Date
Submission Date November 30, 2023
Acceptance Date March 19, 2024
Published in Issue Year 2024 Volume: 9 Issue: 1

Cite

APA Boran, B., Baycan, B., Gül, Ç., Tosunoğlu, M. (2024). Genotoxicological Potentials of Different Habitat Types in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Thrace Region. Journal of Anatolian Environmental and Animal Sciences, 9(1), 110-118. https://doi.org/10.35229/jaes.1398305
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