Determination Of Pigment Content And Antioxidant Activities Of Some Chlorophyta Species Isolated From Altınapa Dam Lake (Konya/ Turkey)
Abstract
Due to global warming, the decrease in water resources and the difficulties in reaching clean water are important problems for people today. New studies on water resources and the living creatures living in these water resources provide information about the status of these resources. Algae are necessary organisms for the survival of other living things in the environment because they provide oxygen to the environment they live in and form the first step of the food chain. Algae are very important living things because they are the primary producers in the aquatic ecosystems they are in. Due to these importance, many studies are carried out on microalgae. In this study, it was aimed to determine the chlorophyll-a, chlorophyll-b, total carotenoid content and antioxidant activities of some green algae species isolated from the benthic algae flora of Altınapa Dam Lake, which is one of the important drinking water sources of the city of Konya. Three Chlorophyta (Chlorella vulgaris, Acutodesmus obliquus, Monoraphidium minutum) species showing high growth potential under culture conditions were selected and studies were carried out with these species. Since Monoraphidium minutum species belonging to Chlorophyta, gives IC50 values close to the reference antioxidant, it has been concluded that it can provide an economic contribution in the food and pharmaceutical industries when produced under appropriate conditions.
Supporting Institution
Selçuk University Scientific Research Project (S.U. BAP) and TUBITAK-BIDEB (2211).
Project Number
14101009
Thanks
This work was supported by Selçuk University Scientific Research Project (S.U. BAP) [Project number: 14101009] and TUBITAK-BIDEB (2211). In addition, this work is part of my doctoral thesis.
References
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- Bors, W., Saran, M., Elstner, E.F. (1992). Screening for Plant Antioxidants. In: Linskens, H.F., Jackson, J.F. (eds) Plant Toxin Analysis. Modern Methods of Plant Analysis, vol 13. Springer, Berlin, Heidelberg. Doi: 10.1007/978-3-662-02783-7_11
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- Gümüş, N. E., Aşıkkutlu, B., Keskinkaya, H. B., & Akköz, C. (2021). Comparison of heavy metal absorption of some algae isolated from Altınapa Dam Lake (Konya). Journal of Anatolian Environmental and Animal Sciences, 6(1), 50-56. Doi: 10.35229/jaes.809876
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- Katırcıoğlu, H., Aslım, B., Türker, A. R., Atıcı, T., & Beyatlı, Y. (2008). Removal of cadmium (II) ion from aqueous system by dry biomass, immobilized live and heat-inactivated Oscillatoria sp. H1 isolated from freshwater (Mogan Lake). Bioresource Technology, 99(10), 4185-4191. Doi: 10.1016/j.biortech.2007.08.068
- Lee, R.E. (2008). Phycology. Cambridge University Press, 614 pp.
- Lichtenthaler, H.K. & Wellburn, A.R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11(5), 591-592.
- Moss, B. (2010). Ecology of fresh waters: a view for the twenty-first century. John Wiley & Sons, 480 pp.
- McCormick, P.V. & Cairns, J. (1994). Algae as indicators of environmental change. Journal of Applied Phycology, 6(5), 509-526.
- Prescott, G.W. (1973). Algae of the western Great Lakes Area. WC Brown Company Dubuque, 680-974.
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- Rippka, R. (1988). [1] Isolation and purification of cyanobacteria. Methods in Enzymology, 167, 3-27.
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- Yim, J.H., Seo, Y.B., Kim, S.M. & Jeon, Y.J. (2021). Recent Research Trends of Cryopreservation Technology Based on Microalgae Chlorophyta. Journal of Life Science, 31(10), 960-968. DOI: 10.5352/JLS.2021.31.10.960
Altınapa Baraj Gölü'nden (Konya/ Türkiye) İzole Edilen Bazı Chlorophyta Türlerinin Pigment İçeriği Ve Antioksidan Aktivitelerinin Belirlenmesi
Abstract
Küresel ısınmaya bağlı olarak su kaynaklarının azalması ve temiz suya ulaşmada yaşanan zorluklar günümüzde önemli sorunlar olarak insanların karşısına çıkmaktadır. Önemi bu gibi nedenlerle daha da artan su kaynakları ve bu su kaynaklarında yaşayan canlılarla ilgili yapılan yeni çalışmalar, bu kaynakların durumu hakkında bilgiler sağlamaktadır. Algler yaşadıkları ortama oksijen sağlamaları ve besin zincirinin ilk basamağını oluşturmaları nedeniyle ortamda bulunan diğer canlıların yaşamlarını sürdürebilmesi için gerekli organizmalardır. Algler, içinde bulundukları sucul ekosistemlerde birincil üretici olmaları nedeniyle oldukça önemli canlılardır. Bu önemlerinden dolayı mikroalgler üzerinde birçok çalışma yapılmaktadır. Bu çalışmada, Konya şehrinin önemli içme suyu kaynaklarından biri olan Altınapa Baraj Gölü'nün bentik alg florasından izole edilen bazı yeşil alg türlerinin klorofil-a, klorofil-b, toplam karotenoid içeriği ve antioksidan aktivitelerinin belirlenmesi amaçlanmıştır. Kültür koşullarında yüksek çoğalma potansiyeli gösteren üç Chlorophyta (Chlorella vulgaris, Acutodesmus obliquus, Monoraphidium minutum) türü seçilmiş ve çalışmalar bu türlerle gerçekleştirilmiştir. Chlorophyta bölümüne ait Monoraphidium minutum türü referans alınan antioksidana yakın IC50 değerleri verdiğinden, uygun koşullarda üretildiğinde gıda ve eczacılık endüstrilerinde ekonomik katkı sağlayabileceği sonucuna varılmıştır.
Project Number
14101009
References
- Apt, K.E., & Behrens, P.W. (1999). Commercial developments in microalgal biotechnology. Journal of Phycology, 35(2), 215-226. Doi: 10.1046/j.1529-8817.1999.3520215.x
- Bors, W., Saran, M., Elstner, E.F. (1992). Screening for Plant Antioxidants. In: Linskens, H.F., Jackson, J.F. (eds) Plant Toxin Analysis. Modern Methods of Plant Analysis, vol 13. Springer, Berlin, Heidelberg. Doi: 10.1007/978-3-662-02783-7_11
- Brand-Williams, W., Cuvelier, M.E. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25-30.
- Chapman, R.L. (2013). Algae: the world’s most important “plants”-an introduction. Mitigation and Adaptation Strategies for Global Change, 18(1), 5-12. Doi: 10.1007/s11027-010-9255-9
- Dere, Ş., Güneş, T. & Sıvacı, R. (1998). Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22(1), 13-18.
- El-Agbar, Z.A., Shakya, A.K., Khalaf, N.A., & Al-Haroon, M. (2008). Comparative antioxidant activity of some edible plants. Turkish Journal of Biology, 32(3), 193-196.
- Google. (2016). Google Earth. https://www.google.com/earth/. https://earth.google.com/web/; searched on 10 December 2016.
- Gökpınar, Ş., Oya, I., Göksan, T., Durmaz, Y., Leyla, U., Burcu, A., Önalan, S.K. & Akdoğan, P. (2013). Studies in Algal Biotechnology. Yunus Research Bulletin, 13(4), 21-26.
- Gümüş, N. E., Aşıkkutlu, B., Keskinkaya, H. B., & Akköz, C. (2021). Comparison of heavy metal absorption of some algae isolated from Altınapa Dam Lake (Konya). Journal of Anatolian Environmental and Animal Sciences, 6(1), 50-56. Doi: 10.35229/jaes.809876
- Guiry M.D. & Guiry G.M. (2021). AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org; searched on 20 May 2021.
- John, D.M., Whitton, B.A., Brook, A.J. (2002). The freshwater algal flora of the British Isles: an identification guide to freshwater and terrestrial algae. Cambridge University Press, 26-613.
- Katırcıoğlu, H., Aslım, B., Türker, A. R., Atıcı, T., & Beyatlı, Y. (2008). Removal of cadmium (II) ion from aqueous system by dry biomass, immobilized live and heat-inactivated Oscillatoria sp. H1 isolated from freshwater (Mogan Lake). Bioresource Technology, 99(10), 4185-4191. Doi: 10.1016/j.biortech.2007.08.068
- Lee, R.E. (2008). Phycology. Cambridge University Press, 614 pp.
- Lichtenthaler, H.K. & Wellburn, A.R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11(5), 591-592.
- Moss, B. (2010). Ecology of fresh waters: a view for the twenty-first century. John Wiley & Sons, 480 pp.
- McCormick, P.V. & Cairns, J. (1994). Algae as indicators of environmental change. Journal of Applied Phycology, 6(5), 509-526.
- Prescott, G.W. (1973). Algae of the western Great Lakes Area. WC Brown Company Dubuque, 680-974.
- Pulz, O. & Gross, W. (2004). Valuable products from biotechnology of microalgae. Applied Microbiology and Biotechnology, 65, 635–648. Doi: 10.1007/s00253-004-1647-x
- Rippka, R. (1988). [1] Isolation and purification of cyanobacteria. Methods in Enzymology, 167, 3-27.
- Wijffels, R.H., Kruse, O. & Hellingwerf, K. J. (2013). Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae. Current Opinion in Biotechnology, 24(3), 405-413. Doi: 10.1016/j.copbio.2013.04.004
- Yim, J.H., Seo, Y.B., Kim, S.M. & Jeon, Y.J. (2021). Recent Research Trends of Cryopreservation Technology Based on Microalgae Chlorophyta. Journal of Life Science, 31(10), 960-968. DOI: 10.5352/JLS.2021.31.10.960
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Project Number | 14101009 |
| Early Pub Date | June 16, 2022 |
| Publication Date | June 30, 2022 |
| Submission Date | March 31, 2022 |
| Acceptance Date | June 13, 2022 |
| Published in Issue | Year 2022 Volume: 7 Issue: 2 |
